##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132787_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1376513 Sequences flagged as poor quality 0 Sequence length 100 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.112172569383652 31.0 25.0 33.0 16.0 34.0 2 28.39715789098977 31.0 26.0 34.0 16.0 34.0 3 29.448790530855867 31.0 28.0 34.0 16.0 34.0 4 34.86996054523277 35.0 35.0 37.0 32.0 37.0 5 33.980972210215235 37.0 35.0 37.0 28.0 37.0 6 35.33655984360482 37.0 35.0 37.0 32.0 37.0 7 35.684804284449186 37.0 35.0 37.0 33.0 37.0 8 35.952581631993304 37.0 35.0 37.0 35.0 37.0 9 37.918661865162186 39.0 38.0 39.0 35.0 39.0 10-11 37.913938335489746 39.0 38.0 39.0 35.0 39.0 12-13 37.87714609306269 39.0 38.0 39.0 35.0 39.0 14-15 39.290655446043736 41.0 39.0 41.0 36.0 41.0 16-17 39.147784292629275 40.5 39.0 41.0 36.0 41.0 18-19 38.706684571812985 40.0 38.0 41.0 35.5 41.0 20-21 38.475781921420285 40.0 38.0 41.0 35.0 41.0 22-23 38.247688906679414 40.0 38.0 41.0 34.5 41.0 24-25 37.98262965914597 40.0 38.0 41.0 34.0 41.0 26-27 37.70697806704332 40.0 37.0 41.0 33.5 41.0 28-29 37.53106690601541 40.0 37.0 41.0 33.0 41.0 30-31 37.374046231310565 40.0 36.5 41.0 33.0 41.0 32-33 37.21944398636264 40.0 36.0 41.0 33.0 41.0 34-35 37.120064612539075 40.0 36.0 41.0 33.0 41.0 36-37 36.91207238870973 39.5 35.5 41.0 33.0 41.0 38-39 36.35971690786792 39.0 35.0 41.0 32.5 41.0 40-41 36.08800752335793 39.0 35.0 41.0 31.0 41.0 42-43 35.92094262822073 39.0 35.0 41.0 31.0 41.0 44-45 35.75670988940897 39.0 35.0 41.0 30.5 41.0 46-47 35.61140468706071 38.0 35.0 41.0 30.0 41.0 48-49 35.428566239476126 38.0 35.0 41.0 30.0 41.0 50-51 35.11048061296914 38.0 35.0 41.0 28.0 41.0 52-53 34.58168248320212 38.0 35.0 40.0 25.0 41.0 54-55 33.61790044845199 37.0 34.0 40.0 13.5 41.0 56-57 32.84753685580884 37.0 34.0 40.0 2.0 41.0 58-59 32.48840657516493 37.0 33.0 40.0 2.0 41.0 60-61 32.23746561056815 36.0 33.0 40.0 2.0 41.0 62-63 31.975021667067438 36.0 33.0 40.0 2.0 41.0 64-65 31.70017173829815 35.0 33.0 39.5 2.0 41.0 66-67 31.39110818423073 35.0 33.0 39.0 2.0 41.0 68-69 31.094501831802532 35.0 33.0 39.0 2.0 41.0 70-71 30.792367017238487 35.0 33.0 38.5 2.0 40.0 72-73 30.495583405314733 35.0 32.0 37.0 2.0 40.0 74-75 30.185278308305115 35.0 32.0 37.0 2.0 39.5 76-77 29.874843172567203 35.0 32.0 36.0 2.0 39.0 78-79 29.554290079352683 35.0 31.0 36.0 2.0 39.0 80-81 29.25284505122727 35.0 31.0 35.5 2.0 37.5 82-83 28.961492190774806 35.0 31.0 35.0 2.0 37.0 84-85 28.683318646463928 35.0 31.0 35.0 2.0 37.0 86-87 28.47844517269361 35.0 30.0 35.0 2.0 36.0 88-89 28.2506049706759 35.0 30.0 35.0 2.0 36.0 90-91 28.044072958264834 35.0 30.0 35.0 2.0 36.0 92-93 27.87360816788508 35.0 30.0 35.0 2.0 35.5 94-95 27.66015831307078 34.5 29.0 35.0 2.0 35.0 96-97 27.44027081473259 34.0 29.0 35.0 2.0 35.0 98-99 27.265301526393138 34.0 29.0 35.0 2.0 35.0 100 27.15847943317644 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18774114579145973 1101 2 2.177160637573376 1101 3 1.3315205935757142 1101 4 0.7149129643605434 1101 5 2.2470282358971474 1101 6 0.9898251863666374 1101 7 0.5520815910174548 1101 8 0.40373975051610955 1101 9 0.35801391559120077 1101 10-11 0.4505223704195984 1101 12-13 0.44684314032449635 1101 14-15 0.5576843478317102 1101 16-17 0.44403968756738976 1101 18-19 0.423210814581104 1101 20-21 0.40339208476988375 1101 22-23 0.45302889374289634 1101 24-25 0.32655996942830257 1101 26-27 0.20240570272208203 1101 28-29 0.2552046360752911 1101 30-31 0.2861523124855836 1101 32-33 0.3417615643377019 1101 34-35 0.307173760090798 1101 36-37 0.3150075964285719 1101 38-39 0.25070565242503307 1101 40-41 0.25797512743172746 1101 42-43 0.3542507620004045 1101 44-45 0.42739801229004826 1101 46-47 0.36218986518071006 1101 48-49 0.25176114167653907 1101 50-51 0.31769288175040344 1101 52-53 0.3514209250142173 1101 54-55 0.2648225350824589 1101 56-57 0.21742142610735726 1101 58-59 -0.034576667672027384 1101 60-61 -0.0237555585736402 1101 62-63 -0.050549139546212984 1101 64-65 -0.019936291070408174 1101 66-67 -0.0026337203895998584 1101 68-69 0.011856260502781879 1101 70-71 -0.03150962741403163 1101 72-73 0.029175411845844934 1101 74-75 0.02270092887897235 1101 76-77 -0.09507764066193403 1101 78-79 0.03398760502054543 1101 80-81 0.057647302871380646 1101 82-83 0.075307128024523 1101 84-85 0.1227305713533049 1101 86-87 0.15254474716193656 1101 88-89 0.09715731593272636 1101 90-91 0.015574093595848382 1101 92-93 0.1421830577301364 1101 94-95 0.2008994580692054 1101 96-97 0.205019299699277 1101 98-99 0.23165815477672425 1101 100 0.3175899507020681 1102 1 1.2498015933357784 1102 2 2.8275820267044445 1102 3 3.000047184682952 1102 4 1.2660754171359017 1102 5 2.5116170182278665 1102 6 1.1144837158638765 1102 7 0.6713783070883679 1102 8 0.5158728513588855 1102 9 0.33504094261822814 1102 10-11 0.4603814230205927 1102 12-13 0.3678326782495205 1102 14-15 0.4408723745681229 1102 16-17 0.32414721444910555 1102 18-19 0.23678971606322818 1102 20-21 0.2114493355690712 1102 22-23 0.04250869612592112 1102 24-25 0.029923817146986664 1102 26-27 0.007055513823502224 1102 28-29 0.013043926979101172 1102 30-31 0.06362252463321738 1102 32-33 -0.025784207240327817 1102 34-35 0.06911069702773176 1102 36-37 0.020986962891811345 1102 38-39 0.030994812552904705 1102 40-41 0.10923929482492412 1102 42-43 0.15500635630438353 1102 44-45 0.19545962228714586 1102 46-47 0.22733081257972287 1102 48-49 0.27752400131036126 1102 50-51 0.2598245295272079 1102 52-53 0.30517710054459 1102 54-55 0.30194837059539026 1102 56-57 0.3863621410158089 1102 58-59 0.3211048202675286 1102 60-61 0.2979023896972137 1102 62-63 0.2516013980881908 1102 64-65 0.27733194490750535 1102 66-67 0.1993569799881989 1102 68-69 0.11495129101717083 1102 70-71 0.22120760594836497 1102 72-73 0.15970432785863053 1102 74-75 0.22688575887024953 1102 76-77 0.32976252208145596 1102 78-79 0.38722067680200567 1102 80-81 0.3671358236502229 1102 82-83 0.410129853976283 1102 84-85 0.34752714211762026 1102 86-87 0.31786006247724785 1102 88-89 0.3462854763512091 1102 90-91 0.30211870272110275 1102 92-93 0.29832952677983116 1102 94-95 0.22483871991169124 1102 96-97 0.2828600436690536 1102 98-99 0.337928134433799 1102 100 0.3629256089409516 1103 1 1.3912727157409392 1103 2 3.013461757456586 1103 3 3.164385227932886 1103 4 1.3724006225944692 1103 5 2.5530262381638096 1103 6 1.1877899955254776 1103 7 0.7019653767221357 1103 8 0.5323294276132486 1103 9 0.43566907608217775 1103 10-11 0.48179986326425706 1103 12-13 0.4385722361580733 1103 14-15 0.5917543453345431 1103 16-17 0.443972456156871 1103 18-19 0.3806662977828523 1103 20-21 0.3205356142268485 1103 22-23 0.33623256649880773 1103 24-25 0.28399144141201305 1103 26-27 0.31586639155590035 1103 28-29 0.3586012629793842 1103 30-31 0.41455422185764235 1103 32-33 0.47580610994940997 1103 34-35 0.5119851279618644 1103 36-37 0.5047072641133212 1103 38-39 0.5461584079524258 1103 40-41 0.6446085625726283 1103 42-43 0.6001659735872593 1103 44-45 0.6583129357422948 1103 46-47 0.7129284502476523 1103 48-49 0.8283444413941652 1103 50-51 0.8719039691704467 1103 52-53 0.9131196706681877 1103 54-55 0.7629400555880324 1103 56-57 0.8450578578482606 1103 58-59 0.9645426008502298 1103 60-61 0.9811105548745616 1103 62-63 0.8874683022025707 1103 64-65 0.8750396016666748 1103 66-67 0.9433890614540807 1103 68-69 0.9740213040111101 1103 70-71 1.0452878523685243 1103 72-73 1.0303029786450537 1103 74-75 1.05885606055649 1103 76-77 0.9633267658768041 1103 78-79 0.8577361403362467 1103 80-81 0.8038804229850953 1103 82-83 0.8428540459445735 1103 84-85 0.7598043722330772 1103 86-87 0.7276710461688296 1103 88-89 0.7463707084297013 1103 90-91 0.8449734289653321 1103 92-93 0.844928980625312 1103 94-95 0.8945350446152389 1103 96-97 1.0060239290621134 1103 98-99 0.9469259278803186 1103 100 1.0048832141147344 1104 1 5.899684119693195 1104 2 4.897922223901972 1104 3 3.973710618652337 1104 4 1.7975051441692216 1104 5 2.767591995533074 1104 6 1.2462429443307883 1104 7 0.7446849159919111 1104 8 0.5270099279100009 1104 9 0.4792370975360285 1104 10-11 0.5161334970101024 1104 12-13 0.3815366482654312 1104 14-15 0.5070230679951706 1104 16-17 0.37370626135766827 1104 18-19 0.3112200093428683 1104 20-21 0.2968809860422894 1104 22-23 0.26135247505616377 1104 24-25 0.17886098178086485 1104 26-27 0.3283302871002718 1104 28-29 0.30824494442487804 1104 30-31 0.32829171993427053 1104 32-33 0.35016919877626407 1104 34-35 0.4278680110427757 1104 36-37 0.39773212508093536 1104 38-39 0.41537872756202887 1104 40-41 0.48647610263373764 1104 42-43 0.58362013136977 1104 44-45 0.6148671308931952 1104 46-47 0.4757776797994495 1104 48-49 0.534773122729753 1104 50-51 0.6064042219565948 1104 52-53 0.6102502182249552 1104 54-55 0.7806412403856982 1104 56-57 0.6863138752575324 1104 58-59 0.7630039025889559 1104 60-61 0.8131409771373299 1104 62-63 0.8625343563207863 1104 64-65 0.7891516628629098 1104 66-67 0.6944443886472733 1104 68-69 0.5991034045540502 1104 70-71 0.5902420211417407 1104 72-73 0.524802791803122 1104 74-75 0.4515279001801673 1104 76-77 0.5910220156954544 1104 78-79 0.6535719832131726 1104 80-81 0.549846615586155 1104 82-83 0.5053326690294462 1104 84-85 0.47027631297121175 1104 86-87 0.5124295804488277 1104 88-89 0.5010191158769999 1104 90-91 0.5728788210036697 1104 92-93 0.6667274990787035 1104 94-95 0.5719772884769334 1104 96-97 0.6058867651028237 1104 98-99 0.6688444647980845 1104 100 0.6031290980968862 1105 1 1.4472583553086693 1105 2 2.927622638035377 1105 3 3.0549671170222332 1105 4 1.2979113473589265 1105 5 2.437072125941036 1105 6 1.1040894506309016 1105 7 0.6761749151107779 1105 8 0.4648855116618691 1105 9 0.28420934178662804 1105 10-11 0.32504881994604773 1105 12-13 0.2825442260255855 1105 14-15 0.2916067317540936 1105 16-17 0.19623996476766337 1105 18-19 0.23807567944596286 1105 20-21 0.29656025293805754 1105 22-23 0.1861947519087508 1105 24-25 0.2356325035008382 1105 26-27 0.2650991654155419 1105 28-29 0.2940877499584076 1105 30-31 0.2568268331601047 1105 32-33 0.292864590440729 1105 34-35 0.32548630340333773 1105 36-37 0.27799558441655847 1105 38-39 0.43692071364009877 1105 40-41 0.45356879802540107 1105 42-43 0.3697739775236215 1105 44-45 0.48879787368991146 1105 46-47 0.3370628150536561 1105 48-49 0.253181793097184 1105 50-51 0.251667240724764 1105 52-53 0.3498039233972179 1105 54-55 0.36040465566458124 1105 56-57 0.47666721035313486 1105 58-59 0.18884098074561706 1105 60-61 0.2085498237317438 1105 62-63 0.20708541808834724 1105 64-65 0.17866615753203874 1105 66-67 0.15035195759607944 1105 68-69 0.2697391433856673 1105 70-71 0.307373486469082 1105 72-73 0.36715762482806014 1105 74-75 0.42587718205522407 1105 76-77 0.3286403080560163 1105 78-79 0.35480072583366606 1105 80-81 0.3860661062901798 1105 82-83 0.3253591030764973 1105 84-85 0.3304402040629384 1105 86-87 0.32127309089027634 1105 88-89 0.2521526959281033 1105 90-91 0.16932784734959938 1105 92-93 0.34073930628638394 1105 94-95 0.3765116086813549 1105 96-97 0.3713105909905643 1105 98-99 0.349739697858265 1105 100 0.35117738428949963 1106 1 -0.5370793599935126 1106 2 1.9570664316923683 1106 3 2.6553098650807208 1106 4 1.1568162890049862 1106 5 2.4479771543587603 1106 6 1.1373945831746184 1106 7 0.6587034412941293 1106 8 0.40995688135252806 1106 9 0.3337147433224814 1106 10-11 0.48646580115998006 1106 12-13 0.4230058512587789 1106 14-15 0.4325803097835106 1106 16-17 0.3840474618391241 1106 18-19 0.21916705000433723 1106 20-21 0.11883887673951676 1106 22-23 0.11708546300251044 1106 24-25 0.14647353781902694 1106 26-27 0.08859150781650982 1106 28-29 0.2045209574728304 1106 30-31 0.19611595633247703 1106 32-33 0.17568597097784533 1106 34-35 0.06433180646203596 1106 36-37 0.1290390136325783 1106 38-39 0.16009308554343704 1106 40-41 0.10709823531117735 1106 42-43 0.21477918382323935 1106 44-45 0.31378160400359434 1106 46-47 0.2697800695983261 1106 48-49 0.3809020846499678 1106 50-51 0.33736293251182303 1106 52-53 0.4072413393371761 1106 54-55 0.7083904684981093 1106 56-57 0.6797573661082694 1106 58-59 0.7329177302792189 1106 60-61 0.649388905687573 1106 62-63 0.5597037529401803 1106 64-65 0.5377099338347016 1106 66-67 0.5066656805341658 1106 68-69 0.5307374513382896 1106 70-71 0.5696679460985692 1106 72-73 0.591846331496459 1106 74-75 0.551177628929274 1106 76-77 0.6047954557847675 1106 78-79 0.5769839008087168 1106 80-81 0.4712682842588549 1106 82-83 0.48941285812010094 1106 84-85 0.46039920445340954 1106 86-87 0.44699871890017207 1106 88-89 0.486762657314717 1106 90-91 0.4538315134522506 1106 92-93 0.4499859113451734 1106 94-95 0.5162204897607943 1106 96-97 0.5662062480689656 1106 98-99 0.5876957913981151 1106 100 0.5758443980428076 1107 1 -1.0525693259706266 1107 2 1.6822269942526376 1107 3 -0.6423653891812364 1107 4 0.03132050092936822 1107 5 1.7773464573444002 1107 6 0.7220105975843083 1107 7 0.34009137499498365 1107 8 0.2533401573745806 1107 9 0.22500752210093822 1107 10-11 0.2804010394111742 1107 12-13 0.25757524339530846 1107 14-15 0.35245936478414563 1107 16-17 0.2277605051591749 1107 18-19 0.18068320632768575 1107 20-21 0.01658713465848649 1107 22-23 0.14909108496637913 1107 24-25 0.04733639101117859 1107 26-27 -0.06803772457618606 1107 28-29 -0.11314274356241327 1107 30-31 -0.06414435514979289 1107 32-33 -0.08368283167121149 1107 34-35 -0.11194132273396207 1107 36-37 -0.09763165572680066 1107 38-39 -0.06379957861245344 1107 40-41 -0.0727511633752087 1107 42-43 -0.026469474364986922 1107 44-45 -0.03749107034096966 1107 46-47 0.029604860821507373 1107 48-49 0.025162769110423255 1107 50-51 0.06366062368589098 1107 52-53 0.11514028954003663 1107 54-55 0.36527782908613915 1107 56-57 0.3275977314449463 1107 58-59 0.3325961503072463 1107 60-61 0.3218355970981577 1107 62-63 0.3205917594172689 1107 64-65 0.1916555426988431 1107 66-67 0.17534506484725298 1107 68-69 0.10620867743584483 1107 70-71 0.2372397671418156 1107 72-73 0.32336799152229645 1107 74-75 0.30202137836070975 1107 76-77 0.2720818575459525 1107 78-79 0.26269292324199256 1107 80-81 0.36271002688571485 1107 82-83 0.43290666304341485 1107 84-85 0.402683104531647 1107 86-87 0.45815963624456657 1107 88-89 0.42171776347704437 1107 90-91 0.33832426312021013 1107 92-93 0.25654782572393486 1107 94-95 0.30002277493445817 1107 96-97 0.41463496375042297 1107 98-99 0.5072769187019368 1107 100 0.45175637519107426 1108 1 -1.8943843601204549 1108 2 -0.09202982385009406 1108 3 1.831196173833817 1108 4 0.7766294469508139 1108 5 0.6321721176429733 1108 6 0.45646760106730966 1108 7 0.17684404102262619 1108 8 0.17507591457071925 1108 9 0.23797741837023523 1108 10-11 0.2829474477475884 1108 12-13 0.3178892747201516 1108 14-15 0.4327262918056931 1108 16-17 0.40660896469977104 1108 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-0.29789104408329337 2215 42-43 -0.22040022486432065 2215 44-45 -0.29362530109967366 2215 46-47 -0.19111458430694483 2215 48-49 -0.09680842025180425 2215 50-51 -0.11277785616307767 2215 52-53 -0.18310042840025886 2215 54-55 -0.215378276416061 2215 56-57 -0.16669417804641995 2215 58-59 -0.13941471182304355 2215 60-61 -0.16555632880952587 2215 62-63 -0.22158620714816735 2215 64-65 -0.1715524110071378 2215 66-67 -0.2015058749869425 2215 68-69 -0.2621310856219665 2215 70-71 -0.21257007717677112 2215 72-73 -0.23268415764730932 2215 74-75 -0.11517129448943919 2215 76-77 -0.10035753888076115 2215 78-79 -0.08019470706252818 2215 80-81 -0.17199646303193106 2215 82-83 -0.2315992057902072 2215 84-85 -0.288350165872469 2215 86-87 -0.22948537456284512 2215 88-89 -0.2730854459130896 2215 90-91 -0.1905534079514979 2215 92-93 -0.28692429389629837 2215 94-95 -0.363372256393351 2215 96-97 -0.45322291197804887 2215 98-99 -0.46813725131639217 2215 100 -0.5656230332742744 2216 1 2.8581106271733745 2216 2 -1.9185372805934584 2216 3 -2.1568909085125227 2216 4 -0.8043627945244509 2216 5 -2.4807156714204694 2216 6 -1.1679748872416056 2216 7 -0.49177532095668397 2216 8 -0.3746106247259178 2216 9 -0.360304936120194 2216 10-11 -0.17820564711836084 2216 12-13 -0.10319646411119976 2216 14-15 -0.10417138000980941 2216 16-17 -0.1667331189869472 2216 18-19 -0.08901135430181029 2216 20-21 -0.04269867739547806 2216 22-23 0.06587473247682851 2216 24-25 0.09146283854964565 2216 26-27 0.10485717728722932 2216 28-29 0.047506173847523314 2216 30-31 0.07968438807805 2216 32-33 0.12525251532421322 2216 34-35 0.009554026680653749 2216 36-37 -0.02588738380921285 2216 38-39 -0.04707489899671913 2216 40-41 0.04513488932506249 2216 42-43 0.0536822983324754 2216 44-45 -0.004153121436921481 2216 46-47 -0.05575534468279386 2216 48-49 -0.058502407841004356 2216 50-51 -0.03821297970607418 2216 52-53 -0.07020829745869861 2216 54-55 0.11513777038881301 2216 56-57 0.2314545978225837 2216 58-59 0.1537356233045628 2216 60-61 0.1556961400166017 2216 62-63 0.16616073195939407 2216 64-65 0.19042246911073235 2216 66-67 0.14834158884503523 2216 68-69 0.025363773344224683 2216 70-71 0.01412228335997412 2216 72-73 -0.09817765169727366 2216 74-75 -0.06995064809764884 2216 76-77 -0.10003619602013458 2216 78-79 -0.06462663977486827 2216 80-81 -0.03912593573134515 2216 82-83 0.015256402343247544 2216 84-85 -0.003848405492099971 2216 86-87 0.04553756235723583 2216 88-89 -0.12718381027340797 2216 90-91 -0.13473274076959996 2216 92-93 -0.11912580886292545 2216 94-95 -0.06605644099149188 2216 96-97 -0.09524753018567012 2216 98-99 -0.2691635372527834 2216 100 -0.3090122287420307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 707.0 3 203.0 4 292.0 5 1091.0 6 3397.0 7 6138.0 8 7312.0 9 9871.0 10 6466.0 11 3599.0 12 3244.0 13 4653.0 14 4752.0 15 5483.0 16 6273.0 17 7214.0 18 10104.0 19 16899.0 20 32777.0 21 38186.0 22 17468.0 23 10932.0 24 10771.0 25 11071.0 26 11838.0 27 12848.0 28 15710.0 29 17736.0 30 20018.0 31 24530.0 32 31700.0 33 45967.0 34 66876.0 35 168534.0 36 164853.0 37 247245.0 38 257189.0 39 72088.0 40 478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.173117202374327 47.56467086735388 23.301882743061846 16.960329187209947 2 13.084889587957296 47.56432377845666 23.053758441190467 16.297028192395572 3 16.04960514940391 27.48959294427049 18.323247146686814 38.13755475963879 4 35.08575654570643 29.12460688711258 19.77467702811379 16.014959539067195 5 14.957115312804673 46.72694593899521 20.61864957187962 17.697289176320506 6 38.299586728822035 26.19614687111287 21.808192185629093 13.69607421443601 7 16.31719734857621 25.781783521302348 42.44254134494953 15.458477785171903 8 37.470187350210274 26.322526558049216 21.140810148542005 15.066475943198501 9 36.71342006940726 27.764503495426485 21.478692900103376 14.043383535062873 10-11 14.180105818107059 49.5787907560626 21.488572937560342 14.752530488269999 12-13 14.006987220607433 27.386846328367405 32.321598125117596 26.284568325907564 14-15 14.404804022918782 38.070145360051086 21.43808304026188 26.086967576768256 16-17 25.732049025327044 26.827534502035217 32.49155656357768 14.948859909060067 18-19 14.690525988494116 27.091026383332377 32.65813690099549 25.56031072717802 20-21 25.816247285713974 38.4128954830067 21.477457895421256 14.29339933585807 22-23 25.27368066992466 27.371772006512106 32.69827455316441 14.656272770398825 24-25 14.412395669347111 38.41877991708033 21.179640148694563 25.98918426487799 26-27 15.24653789921995 31.92473733438672 37.89366242587699 14.935062340516343 28-29 25.013966450008102 27.260912174458213 32.85690000748268 14.86822136805101 30-31 21.826092452450503 29.00085215323066 33.35431630504034 15.818739089278488 32-33 17.23794108737077 30.74417023304538 34.37199648677491 17.645892192808933 34-35 17.37785985312162 30.604324114628778 34.70831732065008 17.309498711599527 36-37 17.571356027876234 30.392230222308108 35.032832962710856 17.00358078710481 38-39 18.365609333148324 30.123471409278373 34.437161145590345 17.073758111982958 40-41 16.643141038261174 31.809906626381295 34.56534736686105 16.981604968496484 42-43 16.709547238552922 30.474737513144774 35.3571292356143 17.458586012688 44-45 16.86071980431714 31.313180478498932 34.15151182734925 17.674587889834676 46-47 17.709531257605267 30.424812551715817 34.73879287736476 17.12686331331415 48-49 16.93383934623211 30.681802496598287 34.69665742350417 17.68770073366543 50-51 17.599361575226677 31.562433482284586 33.6491555110631 17.189049431425637 52-53 17.87090046105681 31.03998767899869 33.55489583632247 17.534216023622033 54-55 17.98944143644121 32.31832899507669 31.12015651141689 18.572073057065207 56-57 18.514935928683567 33.038881579759874 29.88591462630574 18.560267865250818 58-59 19.78375794489409 33.25700520082266 28.374959045065324 18.58427780921793 60-61 19.198765285907214 33.90011572720345 28.123453973918156 18.77766501297118 62-63 18.26539897022366 33.91487545518112 27.904323426049526 19.91540214854569 64-65 18.798551121565872 33.86470015176028 28.801544191736657 18.535204534937193 66-67 18.920271730089 32.97658649064702 29.341132266822033 18.762009512441946 68-69 19.37210182540957 33.296852263654614 28.855012629739058 18.476033281196763 70-71 17.455483529759615 34.55644806841635 29.088028954321537 18.900039447502493 72-73 17.58037466586609 33.64593628871349 29.423871227633143 19.349817817787276 74-75 17.863958753629653 34.232903671534295 28.489897269106123 19.41324030572993 76-77 18.862654131520237 33.31187799808645 28.681666512895358 19.14380135749795 78-79 18.454741999320017 33.6979153045242 28.123083919599452 19.72425877655633 80-81 19.037989746480754 34.46857725700562 27.58951487937186 18.90391811714177 82-83 18.8534552463093 34.41217986764993 27.613892034949483 19.12047285109128 84-85 18.82528621566193 34.63015175478043 26.887381519901606 19.657180509656033 86-87 18.57084479799493 34.936043079884854 27.214827326304704 19.278284795815512 88-89 20.00220847896097 34.01751381933916 26.94620392252017 19.03407377917971 90-91 19.089517563232285 34.8116471196764 26.7845104147294 19.31432490236191 92-93 18.312722073819863 34.968140511567995 26.803560881735226 19.915576532876912 94-95 18.748569755607104 35.11990079280036 26.551583602915485 19.579945848677056 96-97 18.75944877628131 34.1977230674006 26.966743939888165 20.07608421642992 98-99 19.626605468263318 34.358137793028945 25.989815522120672 20.025441216587065 100 18.576577191788235 35.29730558302028 25.88497166390728 20.241145561284203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107797.0 1 57882.0 2 5627.0 3 2708.0 4 1957.5 5 1684.0 6 1667.0 7 1901.0 8 1826.5 9 1581.0 10 1537.0 11 2409.0 12 18201.0 13 19792.0 14 5391.0 15 4455.5 16 4827.5 17 5313.5 18 5866.5 19 6389.0 20 7048.5 21 7862.5 22 8839.0 23 9985.5 24 11123.5 25 12520.5 26 14222.0 27 15650.5 28 17092.5 29 18799.0 30 20656.0 31 22444.0 32 24120.0 33 25810.5 34 27842.5 35 29988.0 36 31746.5 37 33415.5 38 34238.5 39 33841.5 40 33675.0 41 34068.5 42 34031.5 43 33963.5 44 34475.5 45 35215.5 46 36542.5 47 38459.0 48 38955.5 49 41494.5 50 54104.0 51 55036.5 52 40738.5 53 34463.0 54 33210.5 55 33434.0 56 30380.5 57 24906.0 58 21235.5 59 16929.0 60 13156.5 61 10779.0 62 8876.5 63 6669.5 64 4742.0 65 3478.5 66 2673.5 67 2202.5 68 1735.5 69 1244.5 70 922.0 71 724.5 72 544.0 73 389.5 74 276.5 75 212.0 76 168.5 77 111.0 78 59.0 79 31.5 80 20.5 81 9.5 82 2.5 83 1.5 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14355113246296983 2 0.0056664920709067045 3 0.0031238353724229264 4 0.0 5 0.002252067361514203 6 0.012858578160903677 7 0.003269130040907714 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.6323667121196824E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0011260336807571015 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.0897100136359046E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.6323667121196824E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.7220767257555866E-4 74-75 2.905893369695746E-4 76-77 7.264733424239364E-4 78-79 3.632366712119682E-4 80-81 7.264733424239364E-4 82-83 8.717680109087237E-4 84-85 0.0011623573478782984 86-87 3.6323667121196824E-5 88-89 0.0 90-91 7.264733424239365E-5 92-93 0.0 94-95 0.0 96-97 8.717680109087237E-4 98-99 4.7220767257555866E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1376513.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.783367821323615 #Duplication Level Percentage of deduplicated Percentage of total 1 91.05021668783615 54.43288594460126 2 6.040526777585547 7.222460683579028 3 1.2303532631387664 2.206639850411719 4 0.4676521699083692 1.1183128674428868 5 0.22007569373428473 0.6578433073524855 6 0.1464225697339212 0.525218060624785 7 0.09880846205086939 0.4134971841452494 8 0.068861669457429 0.3293426011171107 9 0.06473769471002211 0.3483213673278427 >10 0.5452494298564357 6.74046451062416 >50 0.03873965627288197 1.5896113281898963 >100 0.022879465789107458 2.8343508446582786 >500 0.0031641768462571116 1.2883601712452477 >1k 0.001703787532599983 2.2913719919357254 >5k 2.433982189428547E-4 1.2645690160613086 >10k+ 3.650973284142821E-4 16.736750270683125 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 118784 8.629340950648487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 99651 7.239379504588769 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 11605 0.8430723138829782 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9392 0.6823037632045611 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7989 0.5803795532624828 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3860 0.28041871017563946 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 3801 0.27613251745533823 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3242 0.2355226576138402 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2762 0.20065193717749125 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2664 0.19353249842173667 No Hit ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2377 0.1726827134941697 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2305 0.16745210542871736 No Hit AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2020 0.14674761516963516 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1919 0.13941023441115338 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA 1734 0.12597047757631058 No Hit AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1536 0.11158630539631663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013802993506054793 0.0 0.0 0.0 0.0 2 0.0013802993506054793 0.0 0.0 0.0 0.0 3 0.0013802993506054793 0.0 0.0 0.0 0.0 4 0.0013802993506054793 0.0 0.0 0.0 0.0 5 0.0013802993506054793 0.0 0.0 0.0 0.0 6 0.0013802993506054793 0.0 0.0 0.0 0.0 7 0.0013802993506054793 0.0 0.0 0.0 0.0 8 0.0013802993506054793 0.0 0.0 0.0 0.0 9 0.0013802993506054793 0.0 0.0 0.0 0.0 10-11 0.001452946684847873 0.0 0.0 0.0 0.0 12-13 0.0015619176862114634 0.0 0.0 0.0 0.0 14-15 0.0017435360218174473 0.0 0.0 0.0 0.0 16-17 0.0017798596889386442 0.0 0.0 0.0 0.0 18-19 0.0018888306903022346 0.0 0.0 0.0 0.0 20-21 0.0019614780245446285 0.0 0.0 0.0 0.0 22-23 0.0019614780245446285 0.0 0.0 0.0 0.0 24-25 0.002034125358787022 0.0 0.0 0.0 0.0 26-27 0.002034125358787022 0.0 0.0 0.0 0.0 28-29 0.002070449025908219 0.0 0.0 0.0 0.0 30-31 0.0021430963601506126 0.0 0.0 0.0 0.0 32-33 0.0022157436943930063 0.0 0.0 0.0 0.0 34-35 0.002470009364241384 0.0 0.0 0.0 0.0 36-37 0.0031964827066653205 0.0 0.0 0.0 0.0 38-39 0.004467811055907209 0.0 0.0 0.0 0.0 40-41 0.007264733424239364 0.0 0.0 0.0 0.0 42-43 0.010461216130904685 0.0 0.0 0.0 0.0 44-45 0.014166230177266759 0.0 0.0 0.0 0.0 46-47 0.017762273222265243 0.0 0.0 0.0 0.0 48-49 0.02331979429180836 0.0 0.0 0.0 0.0 50-51 0.029131581031199852 0.0 0.0 0.0 0.0 52-53 0.035597193778772886 0.0 0.0 0.0 0.0 54-55 0.0445691395577085 0.0 0.0 0.0 0.0 56-57 0.05677389171043063 0.0 0.0 0.0 0.0 58-59 0.07003203020966747 0.0 0.0 0.0 3.6323667121196824E-5 60-61 0.0856512070717821 0.0 0.0 0.0 7.264733424239365E-5 62-63 0.10562922398844035 0.0 0.0 0.0 7.264733424239365E-5 64-65 0.12996608095964224 0.0 0.0 0.0 7.264733424239365E-5 66-67 0.15768103897311542 0.0 0.0 0.0 7.264733424239365E-5 68-69 0.19059028138491974 0.0 0.0 0.0 7.264733424239365E-5 70-71 0.23948193733005063 0.0 0.0 0.0 7.264733424239365E-5 72-73 0.29284140433108874 0.0 0.0 0.0 7.264733424239365E-5 74-75 0.35346560475636624 0.0 0.0 0.0 7.264733424239365E-5 76-77 0.418739234573157 0.0 0.0 0.0 7.264733424239365E-5 78-79 0.4944740805208523 0.0 0.0 0.0 7.264733424239365E-5 80-81 0.5904048853879331 0.0 0.0 0.0 7.264733424239365E-5 82-83 0.710708870893337 0.0 0.0 0.0 7.264733424239365E-5 84-85 0.8518626413263078 0.0 0.0 0.0 7.264733424239365E-5 86-87 1.010233829974726 0.0 0.0 0.0 7.264733424239365E-5 88 1.1351872448716431 0.0 0.0 0.0 1.452946684847873E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2310 0.0 87.852516 1 AACGTGT 965 0.0 74.653984 1 ACGTGTG 1020 0.0 73.74718 2 CGTGTGG 1045 0.0 73.32725 3 GTGGAAT 40150 0.0 70.57489 6 TGGAATC 40545 0.0 69.80761 7 AACGAGT 34875 0.0 69.356026 1 ACGAGTG 38075 0.0 69.02358 2 GGAATCA 41035 0.0 68.994446 8 GAATCAC 41040 0.0 68.98604 9 AGTGGAA 40090 0.0 68.58416 5 CGAGTGG 39360 0.0 68.461296 3 GAGTGGA 40625 0.0 67.77353 4 ATCGAGT 800 0.0 62.38853 1 TCGAGTG 845 0.0 59.5323 2 AGCGAGT 410 0.0 58.569992 1 TGAGTGG 1270 0.0 57.374912 3 GCGAGTG 415 0.0 56.643166 2 GTGTGGA 1540 0.0 53.418983 4 ATGAGTG 1335 0.0 50.35938 2 >>END_MODULE