FastQCFastQC Report
Thu 26 May 2016
SRR2132786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594961
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16305310.223008587670796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1399898.7769544208291No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126840.795254554813566No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG117740.7381998682099437No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110750.6943743452034251No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53960.33831548232213826No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC49330.3092865593578777No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45050.2824520474168334No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39880.2500374617310392No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37990.23818764220567148No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33450.20972299636166652No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31350.19655653022236907No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA29980.18796697850292263No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27900.17492590727923754No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27750.17398544541214486No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20430.12809090629802233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT27150.088.252371
AACGTGT13700.084.870121
CGTGTGG15750.079.6885453
ACGTGTG15550.078.6023252
TCGAGTG10550.074.859912
AACGAGT441700.073.1097261
ATCGAGT10500.073.076331
GTGGAAT523450.073.063586
ACGAGTG479750.072.4138952
TGGAATC528550.072.336087
CGAGTGG497550.071.944633
AGCGAGT4650.071.8759841
GAATCAC534750.071.471049
AGTGGAA516350.071.3279955
GGAATCA536250.071.236068
GAGTGGA517650.071.2033544
AATGAGT15600.065.1789861
ATGAGTG17600.061.968042
TGAGTGG18700.061.8384673
GTGTGGA23500.058.2090264
Produced by FastQC (version 0.11.5)