##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132786_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1594961
Sequences flagged as poor quality	0
Sequence length	100
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.196755908138194	31.0	25.0	33.0	16.0	34.0
2	28.419981429013	31.0	26.0	34.0	16.0	34.0
3	29.454523966416733	31.0	28.0	34.0	16.0	34.0
4	34.895788047482036	35.0	35.0	37.0	32.0	37.0
5	33.943603636703344	35.0	35.0	37.0	28.0	37.0
6	35.331184273471266	37.0	35.0	37.0	32.0	37.0
7	35.662057567551805	37.0	35.0	37.0	33.0	37.0
8	35.94261677871747	37.0	35.0	37.0	35.0	37.0
9	37.91094327698295	39.0	38.0	39.0	35.0	39.0
10-11	37.89883514393142	39.0	38.0	39.0	35.0	39.0
12-13	37.86391861619187	39.0	38.0	39.0	35.0	39.0
14-15	39.27497318116242	41.0	39.0	41.0	36.0	41.0
16-17	39.094576607202306	40.5	39.0	41.0	36.0	41.0
18-19	38.55639981165684	40.0	38.0	41.0	35.0	41.0
20-21	38.267992446210286	40.0	37.5	41.0	34.5	41.0
22-23	38.00176148507707	40.0	37.0	41.0	34.0	41.0
24-25	37.69162945050067	40.0	37.0	41.0	33.5	41.0
26-27	37.37078273387249	40.0	36.5	41.0	33.0	41.0
28-29	37.17301676968904	40.0	36.0	41.0	33.0	41.0
30-31	37.00404273207934	40.0	35.5	41.0	33.0	41.0
32-33	36.83814651267335	40.0	35.0	41.0	32.0	41.0
34-35	36.75185568800742	40.0	35.0	41.0	32.0	41.0
36-37	36.53117505694496	39.5	35.0	41.0	31.5	41.0
38-39	35.893038763957236	38.0	35.0	41.0	31.0	41.0
40-41	35.59212450962751	38.0	35.0	41.0	30.0	41.0
42-43	35.40973070814898	38.0	35.0	41.0	30.0	41.0
44-45	35.256404702058546	38.0	35.0	41.0	30.0	41.0
46-47	35.11794489018854	38.0	35.0	41.0	29.5	41.0
48-49	34.924384671474726	37.5	35.0	40.0	27.5	41.0
50-51	34.59331701527498	37.0	35.0	40.0	26.5	41.0
52-53	33.963932660422415	37.0	34.5	40.0	21.5	41.0
54-55	32.8490737391071	36.5	33.5	40.0	4.5	41.0
56-57	31.945666696552458	36.0	33.0	40.0	2.0	41.0
58-59	31.556971925959317	35.5	33.0	40.0	2.0	41.0
60-61	31.30779780822227	35.0	33.0	40.0	2.0	41.0
62-63	31.05222071260677	35.0	33.0	40.0	2.0	41.0
64-65	30.7833360815719	35.0	32.0	39.0	2.0	41.0
66-67	30.474814744686547	35.0	32.0	39.0	2.0	41.0
68-69	30.175562599963257	35.0	31.0	38.5	2.0	40.5
70-71	29.86562304658233	35.0	31.0	37.5	2.0	40.0
72-73	29.5655890645602	35.0	31.0	37.0	2.0	40.0
74-75	29.26028159936199	35.0	31.0	36.5	2.0	39.0
76-77	28.944971695232674	35.0	30.0	36.0	2.0	39.0
78-79	28.619035512467075	35.0	30.0	36.0	2.0	39.0
80-81	28.309020095162204	35.0	29.5	35.0	2.0	37.0
82-83	28.0125291464807	35.0	29.0	35.0	2.0	37.0
84-85	27.722744317886143	35.0	29.0	35.0	2.0	36.5
86-87	27.512570213315563	35.0	29.0	35.0	2.0	36.0
88-89	27.27705379629972	35.0	27.5	35.0	2.0	36.0
90-91	27.059218375872515	34.5	27.0	35.0	2.0	36.0
92-93	26.87625622193897	34.0	27.0	35.0	2.0	35.0
94-95	26.661699565067735	34.0	25.5	35.0	2.0	35.0
96-97	26.43280305913436	34.0	24.5	35.0	2.0	35.0
98-99	26.236623027146116	34.0	23.5	35.0	2.0	35.0
100	26.124438779380814	34.0	23.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.06040911989823883
1101	2	2.0982212757447094
1101	3	1.1191431903702167
1101	4	0.6081954139322789
1101	5	2.0919353859550895
1101	6	0.8929058497339994
1101	7	0.5208779578813818
1101	8	0.379158685054648
1101	9	0.2443934344723928
1101	10-11	0.40357196918909466
1101	12-13	0.3510567762994725
1101	14-15	0.45742460650808425
1101	16-17	0.35019237106178025
1101	18-19	0.27275248994479284
1101	20-21	0.26003811410576816
1101	22-23	0.21595292852648384
1101	24-25	-0.009192514678346697
1101	26-27	-0.18466578269590173
1101	28-29	-0.09487954012291766
1101	30-31	-0.015599431668732677
1101	32-33	-0.08292039203519153
1101	34-35	-0.13112995143773531
1101	36-37	-0.049277057574911964
1101	38-39	-0.055778793788533676
1101	40-41	-0.08720549666290367
1101	42-43	-0.022970835582334814
1101	44-45	0.020254866831116658
1101	46-47	0.0029274743355856003
1101	48-49	0.04076196432237822
1101	50-51	0.04406337656002535
1101	52-53	0.0635315031235919
1101	54-55	-0.02186967981474197
1101	56-57	-0.12191049555871558
1101	58-59	-0.1699134388979573
1101	60-61	-0.09250326902368755
1101	62-63	-0.10999477920063327
1101	64-65	-0.019939943789690773
1101	66-67	-0.11627418408844292
1101	68-69	-9.593860805061638E-4
1101	70-71	0.07180010020649163
1101	72-73	0.15851835274393267
1101	74-75	0.16934944627514525
1101	76-77	0.10856133336254103
1101	78-79	0.13310309933319786
1101	80-81	0.1629636907383727
1101	82-83	0.15152911161049332
1101	84-85	0.222222193804555
1101	86-87	0.3657379160803238
1101	88-89	0.3002693117157058
1101	90-91	0.21623507620858007
1101	92-93	0.24612083142138985
1101	94-95	0.24929628680818539
1101	96-97	0.13752592092198057
1101	98-99	0.16883581644355772
1101	100	0.20029776985685288
1102	1	1.4917723042023319
1102	2	2.919819732788717
1102	3	3.037453993806132
1102	4	1.3100291071301342
1102	5	2.492672838919141
1102	6	1.1438915390976376
1102	7	0.6667814780088932
1102	8	0.4390259752290362
1102	9	0.36922292820062097
1102	10-11	0.3737478536113059
1102	12-13	0.3892529251291137
1102	14-15	0.3755897517029041
1102	16-17	0.30900470227047094
1102	18-19	0.07748934777564642
1102	20-21	-0.02003333846437272
1102	22-23	-0.13871345541126345
1102	24-25	-0.12142718191901025
1102	26-27	-0.07102346689602967
1102	28-29	0.03153367256585682
1102	30-31	0.0836928415388698
1102	32-33	0.10365676277687896
1102	34-35	0.13615193011002447
1102	36-37	0.19949974857203756
1102	38-39	0.24157324457316776
1102	40-41	0.2931353055108019
1102	42-43	0.3446095119730188
1102	44-45	0.37860798204243906
1102	46-47	0.33978513749873684
1102	48-49	0.30668978064085906
1102	50-51	0.3544293042086579
1102	52-53	0.34040899288101656
1102	54-55	0.4677404896457702
1102	56-57	0.27691266817182125
1102	58-59	0.1737601305414671
1102	60-61	0.24964077455274492
1102	62-63	0.1876494140737499
1102	64-65	0.18233072413147156
1102	66-67	0.16381098825323193
1102	68-69	0.21566848920250337
1102	70-71	0.1282968236201647
1102	72-73	0.16518501839432176
1102	74-75	0.23777208109324022
1102	76-77	0.30858485514862366
1102	78-79	0.3095891335204328
1102	80-81	0.29941665112969673
1102	82-83	0.3559305362715719
1102	84-85	0.36988496974726814
1102	86-87	0.3713931296849715
1102	88-89	0.3412347202081847
1102	90-91	0.37292248920236304
1102	92-93	0.30918109353610035
1102	94-95	0.36815722259416717
1102	96-97	0.3616958595000881
1102	98-99	0.36559418504687
1102	100	0.3934950193170579
1103	1	1.1934613741532552
1103	2	2.7877385105964123
1103	3	2.9736286445582465
1103	4	1.2751876938642255
1103	5	2.4676243310409163
1103	6	1.1364405050976387
1103	7	0.7135113723551143
1103	8	0.4747603576956081
1103	9	0.3951489381455531
1103	10-11	0.44224663421192645
1103	12-13	0.44041061801016923
1103	14-15	0.4269314298250748
1103	16-17	0.22621016532111327
1103	18-19	0.16448284333024077
1103	20-21	0.0707951224762553
1103	22-23	0.05659122355879731
1103	24-25	0.11185947504117877
1103	26-27	0.29472350434453176
1103	28-29	0.3047616987769004
1103	30-31	0.28937419972410794
1103	32-33	0.3868163830101281
1103	34-35	0.39795924556354834
1103	36-37	0.3658364742652225
1103	38-39	0.3881236023206043
1103	40-41	0.4460461906609936
1103	42-43	0.4017588042853504
1103	44-45	0.42051345634725834
1103	46-47	0.4227686680944984
1103	48-49	0.49592471432662677
1103	50-51	0.3815590990938418
1103	52-53	0.494191045938301
1103	54-55	0.640627620224322
1103	56-57	0.6814950349320377
1103	58-59	0.7731328572410376
1103	60-61	0.6859931500393728
1103	62-63	0.6380491142765017
1103	64-65	0.6867508627523478
1103	66-67	0.7315223444292123
1103	68-69	0.6875112347042176
1103	70-71	0.7057127015612075
1103	72-73	0.646699804679777
1103	74-75	0.632958826849503
1103	76-77	0.6251196780013295
1103	78-79	0.6838763196039004
1103	80-81	0.6889178994010621
1103	82-83	0.7888979663174069
1103	84-85	0.7255567972318673
1103	86-87	0.6991269677084269
1103	88-89	0.6164805445239345
1103	90-91	0.5742559623782277
1103	92-93	0.6093808552538533
1103	94-95	0.5911999230791594
1103	96-97	0.6383368192990915
1103	98-99	0.6334223401801573
1103	100	0.6417808189561001
1104	1	5.768508073242835
1104	2	4.806562591059084
1104	3	3.827754151516835
1104	4	1.6788159186256806
1104	5	2.711131614976992
1104	6	1.2471731619252395
1104	7	0.7020494804950559
1104	8	0.5232836567548986
1104	9	0.40033464065131596
1104	10-11	0.46365669428967493
1104	12-13	0.28413120319323326
1104	14-15	0.3827096357946971
1104	16-17	0.14748110979267182
1104	18-19	0.007589047998472154
1104	20-21	0.0020279784411201263
1104	22-23	0.025580503803823262
1104	24-25	0.08366332203645044
1104	26-27	0.18045223452195813
1104	28-29	0.19290251406167158
1104	30-31	0.22099635500629233
1104	32-33	0.25373547718803025
1104	34-35	0.19990602262517854
1104	36-37	0.21983702960574192
1104	38-39	0.34534695579205277
1104	40-41	0.37928135773995564
1104	42-43	0.35762409774635273
1104	44-45	0.4575423284450437
1104	46-47	0.37334784899527307
1104	48-49	0.47743916720401813
1104	50-51	0.5667864801864582
1104	52-53	0.4834609133858052
1104	54-55	0.608051429187185
1104	56-57	0.49615069575920856
1104	58-59	0.5636605148820166
1104	60-61	0.5712543283709941
1104	62-63	0.6208888115878715
1104	64-65	0.5907682465936226
1104	66-67	0.6539329991139873
1104	68-69	0.5759918454666035
1104	70-71	0.7223105116123065
1104	72-73	0.5981485983567438
1104	74-75	0.6259895837393685
1104	76-77	0.6295302878100983
1104	78-79	0.5681998699688009
1104	80-81	0.6469348569203177
1104	82-83	0.6120824515616192
1104	84-85	0.5933687836120001
1104	86-87	0.5316732881567425
1104	88-89	0.5208462783307084
1104	90-91	0.6150238468673983
1104	92-93	0.6833837665844804
1104	94-95	0.6739748306626403
1104	96-97	0.7690582290786949
1104	98-99	0.7471325197402585
1104	100	0.7457759050338808
1105	1	1.5489517036864626
1105	2	2.9837909182874114
1105	3	3.0713031950658802
1105	4	1.3064797362895035
1105	5	2.46956466341004
1105	6	1.1122484570005327
1105	7	0.703587942033522
1105	8	0.4693154027866413
1105	9	0.3220033423200164
1105	10-11	0.3863632469962184
1105	12-13	0.3350510241130493
1105	14-15	0.31805558614065177
1105	16-17	0.2230875403991064
1105	18-19	0.13919669960612424
1105	20-21	0.08203611844925263
1105	22-23	0.021136059359378123
1105	24-25	-0.05757192419880397
1105	26-27	-0.06639554732582553
1105	28-29	0.01557447173362192
1105	30-31	0.026875475885411504
1105	32-33	0.07945179790435475
1105	34-35	0.06922633194549377
1105	36-37	0.04815001291872534
1105	38-39	0.09383087927596989
1105	40-41	0.05601924947785619
1105	42-43	0.10787643799869784
1105	44-45	0.15986630076901065
1105	46-47	0.14431650996392875
1105	48-49	0.16641759618244834
1105	50-51	0.11353658193656457
1105	52-53	0.24511130003619286
1105	54-55	0.3482732444090004
1105	56-57	0.3982711678796811
1105	58-59	0.3467435429650507
1105	60-61	0.3150394321560981
1105	62-63	0.2812205169195785
1105	64-65	0.30478330560868017
1105	66-67	0.28627732145831075
1105	68-69	0.1964741409489008
1105	70-71	0.34700729630909066
1105	72-73	0.36305905826720775
1105	74-75	0.3431995959493861
1105	76-77	0.3255986638784769
1105	78-79	0.33411765588658326
1105	80-81	0.3492913892768499
1105	82-83	0.25212722160638634
1105	84-85	0.3108962561394719
1105	86-87	0.38911732560077894
1105	88-89	0.38152393400836715
1105	90-91	0.33856882041237313
1105	92-93	0.3185913367920499
1105	94-95	0.29414984083765106
1105	96-97	0.33631708133754756
1105	98-99	0.3456876682954082
1105	100	0.3604116396696142
1106	1	-0.7509765405040767
1106	2	1.8734001251097325
1106	3	2.6645747080095212
1106	4	1.1650534515845266
1106	5	2.3227980905778907
1106	6	1.037068239525233
1106	7	0.5483867497831483
1106	8	0.41574693118538875
1106	9	0.29155735236582814
1106	10-11	0.4146769854738963
1106	12-13	0.3462926756006013
1106	14-15	0.44819606193686923
1106	16-17	0.334668376078298
1106	18-19	0.19882936505845805
1106	20-21	0.1977310458654955
1106	22-23	0.07420196431053228
1106	24-25	-0.034722390611563014
1106	26-27	0.02007599873589072
1106	28-29	0.1599896618865131
1106	30-31	0.1954770655525735
1106	32-33	0.1451160911096281
1106	34-35	0.2706658638768289
1106	36-37	0.2672969467377868
1106	38-39	0.3051296594272088
1106	40-41	0.2886435802169416
1106	42-43	0.3113292723531629
1106	44-45	0.36991984164222913
1106	46-47	0.40002827239700167
1106	48-49	0.5076940216391961
1106	50-51	0.520939021634085
1106	52-53	0.5914462340760451
1106	54-55	0.571744293699723
1106	56-57	0.7284453137095674
1106	58-59	0.7816425328640406
1106	60-61	0.6996709936729033
1106	62-63	0.727622515042885
1106	64-65	0.7185957469621052
1106	66-67	0.6334680815179219
1106	68-69	0.6053001055125691
1106	70-71	0.7151612401187712
1106	72-73	0.6993020896741697
1106	74-75	0.6834719920414543
1106	76-77	0.6533217968475142
1106	78-79	0.623192281928425
1106	80-81	0.5467818678329017
1106	82-83	0.509903619464751
1106	84-85	0.45607260984041886
1106	86-87	0.4536746474531803
1106	88-89	0.44186173828059694
1106	90-91	0.4940264016896272
1106	92-93	0.5423104955931777
1106	94-95	0.5399427425321903
1106	96-97	0.5622096132136889
1106	98-99	0.5842605556387888
1106	100	0.5505915811610329
1107	1	-0.976453320796633
1107	2	1.7474084589351584
1107	3	-0.6223646194728651
1107	4	-0.04433946659325727
1107	5	1.8339932701258164
1107	6	0.8335769373050823
1107	7	0.4177268200248889
1107	8	0.2816819081700217
1107	9	0.3557353795750018
1107	10-11	0.40997802693897967
1107	12-13	0.32513442646401103
1107	14-15	0.44988063009398616
1107	16-17	0.3771903112751147
1107	18-19	0.3985549083444724
1107	20-21	0.34701265245678314
1107	22-23	0.4045526497434082
1107	24-25	0.12161722768356498
1107	26-27	0.057305758923540395
1107	28-29	0.010135229038596094
1107	30-31	0.04305894652749487
1107	32-33	0.002722870074990169
1107	34-35	0.0676624652148945
1107	36-37	0.16349539164386329
1107	38-39	0.13296423585734374
1107	40-41	0.21611681860444776
1107	42-43	0.26378754329779497
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2216	96-97	0.17065185300241836
2216	98-99	0.18110658235510257
2216	100	0.19763565980625586
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	806.0
3	257.0
4	407.0
5	1398.0
6	4633.0
7	8592.0
8	10198.0
9	14180.0
10	9195.0
11	5057.0
12	4578.0
13	5899.0
14	6094.0
15	6726.0
16	7780.0
17	9325.0
18	13242.0
19	22353.0
20	43901.0
21	51395.0
22	23655.0
23	14813.0
24	14431.0
25	14947.0
26	15322.0
27	16588.0
28	19633.0
29	21779.0
30	24715.0
31	29343.0
32	37404.0
33	55408.0
34	79704.0
35	210664.0
36	183864.0
37	259796.0
38	269554.0
39	76554.0
40	771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.08254963689813	50.75313970411875	22.632680047166197	15.531630611816915
2	11.977056379658228	51.01619822605786	21.997145841719618	15.00959955256429
3	14.788952285409742	27.8890839551069	17.54047275691266	39.7814910025707
4	37.20630159608918	29.485172364716128	18.902656553984706	14.405869485209982
5	13.555106355498095	50.393245112336935	19.857686901150768	16.193961631014197
6	40.26724940665239	26.545854718406787	20.89129119337332	12.295604681567504
7	15.122160421490605	26.113557433612282	44.75127452252822	14.013007622368898
8	39.66686332769265	26.714446309345497	20.05986353271334	13.558826830248513
9	38.76809527004109	28.465210121125217	20.275166602819755	12.49152800601394
10-11	12.719339218952689	53.43945714033133	20.553950848954926	13.287252791761054
12-13	12.548833482448787	27.80359519762552	32.9737843119675	26.67378700795819
14-15	12.985301834966497	40.14352075066412	20.49996833778381	26.371209076585572
16-17	26.143836745851466	27.169755247933963	33.15720572477947	13.529202281435095
18-19	13.281108440896046	27.582586658858744	33.35059603338264	25.785708866862578
20-21	26.14935412213841	40.61575800286026	20.491598227166683	12.743289647834649
22-23	25.545076023802466	27.902124252567933	33.45122545316155	13.101574270468056
24-25	13.004675349428608	40.64842337837728	20.092497559501453	26.25440371269266
26-27	13.903447765634322	33.142450863203194	39.42561586948391	13.528485501678569
28-29	25.03387230157979	27.929523041629228	33.60100967986051	13.43559497693047
30-31	21.379739065720102	29.732827323050532	34.250618040190325	14.63681557103904
32-33	16.03622282927294	31.841311480343403	35.65651448530717	16.465951205076486
34-35	16.10957885490617	31.62748180049543	35.89818183642108	16.364757508177316
36-37	16.421686799865327	31.653846081502934	35.95282267089917	15.971644447732578
38-39	17.18587476433593	31.22844383028801	35.56591665877723	16.01976474659882
40-41	15.585710246206647	32.90908053551153	35.64585591748011	15.859353300801713
42-43	15.433688664067624	31.86048305083683	36.50089093925437	16.204937345841174
44-45	15.45169443014594	32.626346349534565	35.407542880358825	16.514416339960665
46-47	16.44789433722831	31.600615939825488	35.93445231576196	16.01703740718425
48-49	15.688471379550972	31.9061720004439	35.835609773530514	16.56974684647462
50-51	16.145629893144722	32.85180640780558	34.74721325474416	16.255350444305535
52-53	16.6290585708213	32.38564902649474	34.639376083884336	16.345916318799617
54-55	17.0089738871358	33.549723159375056	31.968054391298594	17.47324856219055
56-57	17.60049305280819	34.60667690307161	30.14126991192888	17.65156013219132
58-59	18.733780951383764	35.09998050109062	28.658506383541667	17.507732163983945
60-61	18.295368977674062	35.597108644035806	28.360066484384255	17.74745589390587
62-63	17.111688415912756	35.543974627568915	28.204227129278202	19.140109827240128
64-65	17.553846144200392	35.45779489279048	29.279650098027478	17.708708864981652
66-67	17.822698430462697	34.66317817902073	29.752680395533304	17.761442994983263
68-69	18.163484875178764	34.96370757654889	29.222313272863726	17.650494275408615
70-71	16.35488265857284	36.10991115143254	29.64896947323477	17.886236716759846
72-73	16.534583442531886	35.45067757440027	29.72675074014674	18.287988242921113
74-75	16.84393933741642	35.94252120829855	28.644628197624574	18.56891125666046
76-77	17.929149009406547	35.01034199753848	28.79319954882683	18.267309444228136
78-79	17.587702455088788	35.4757586824686	28.116918842410666	18.819620020031945
80-81	17.99058022763196	36.242172917778255	27.66722697927136	18.10001987531843
82-83	17.73921973891816	36.399633716743665	27.584728082332855	18.276418462005324
84-85	17.75518962667166	36.56647145513317	26.850598754245077	18.827740163950104
86-87	17.671038772132217	36.784935045392984	27.12177734864824	18.422248833826554
88-89	19.048772979402003	35.800123012412215	26.918338442131184	18.232765566054592
90-91	18.05450232435369	36.59249654928542	26.898457296251095	18.45454383010979
92-93	17.31017247443668	36.768485248228636	26.995581710148397	18.92576056718628
94-95	17.727555008415568	36.988157386969775	26.544983402410278	18.73930420220438
96-97	17.733012610815262	36.06426070439618	26.891692824904755	19.3110338598838
98-99	18.53018497770148	36.188837386023	25.882457683599913	19.398519952675603
100	17.5103341084829	37.19000025705957	25.732165237896098	19.56750039656142
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152500.0
1	81975.0
2	8055.0
3	3741.5
4	2676.0
5	2413.0
6	2472.0
7	2770.0
8	2628.0
9	2270.5
10	2197.0
11	3374.0
12	25039.0
13	27370.0
14	7732.0
15	6243.5
16	6637.5
17	7402.0
18	7995.5
19	8661.5
20	9637.5
21	10674.0
22	11865.5
23	13239.0
24	14313.5
25	15695.0
26	17470.5
27	19140.0
28	20926.0
29	22918.0
30	24940.0
31	26931.0
32	29011.5
33	30975.5
34	33125.0
35	34961.5
36	36514.5
37	38018.5
38	38710.5
39	38975.5
40	39429.0
41	39331.5
42	37991.0
43	37504.5
44	38547.0
45	39207.5
46	39619.5
47	40998.0
48	41725.0
49	44563.0
50	57674.5
51	58907.5
52	44181.0
53	37307.5
54	36029.5
55	37130.5
56	33731.0
57	25923.0
58	20506.5
59	15757.0
60	12014.5
61	9676.0
62	7937.5
63	6023.5
64	4254.0
65	3048.5
66	2376.5
67	1904.5
68	1454.5
69	1091.0
70	809.0
71	605.5
72	459.5
73	360.5
74	273.0
75	213.0
76	161.5
77	112.5
78	73.0
79	46.0
80	23.5
81	12.0
82	9.0
83	6.0
84	3.0
85	1.0
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14389066566517927
2	0.005956258491586942
3	0.0038245449261768783
4	0.0
5	0.001692831360766815
6	0.012288701730010952
7	0.0031348728903089164
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.1348728903089164E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.40461867092675E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.88092373418535E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	9.40461867092675E-5
64-65	0.0
66-67	3.1348728903089164E-5
68-69	0.0
70-71	0.0
72-73	4.388822046432483E-4
74-75	4.702309335463375E-4
76-77	5.015796624494266E-4
78-79	5.329283913525158E-4
80-81	0.00112855424051121
82-83	0.0012852978850266558
84-85	0.001536087716251369
86-87	6.269745780617833E-5
88-89	0.0
90-91	9.40461867092675E-5
92-93	0.0
94-95	3.1348728903089164E-5
96-97	0.0012852978850266558
98-99	7.5236949367414E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1594961.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.22294179548722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.98458234758363	48.79228771124253
2	6.665373985971835	7.228323713730108
3	1.4462201691035859	2.352549361582903
4	0.5285708081244629	1.1464265665490558
5	0.25299571910097285	0.6859086075659743
6	0.1522819109014612	0.4954303914789301
7	0.09934002994398976	0.377055606313044
8	0.07590418784118294	0.3292598687476953
9	0.06210515740389506	0.3030771901599877
>10	0.6432262803741927	7.539094562349794
>50	0.05335369649160899	1.9507332006468943
>100	0.030269168969953186	3.4150537965775345
>500	0.003350392149783105	1.2521944901003812
>1k	0.001732961456784365	2.5848241057842776
>5k	1.1553076378562432E-4	0.33802798882293544
>10k+	5.776538189281216E-4	21.209752838347917
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	163053	10.223008587670796	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	139989	8.7769544208291	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12684	0.795254554813566	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	11774	0.7381998682099437	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11075	0.6943743452034251	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5396	0.33831548232213826	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	4933	0.3092865593578777	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4505	0.2824520474168334	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3988	0.2500374617310392	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3799	0.23818764220567148	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3345	0.20972299636166652	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3135	0.19655653022236907	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	2998	0.18796697850292263	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2790	0.17492590727923754	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2775	0.17398544541214486	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2043	0.12809090629802233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0012539491561235666	0.0	0.0	0.0	0.0
2	0.0012539491561235666	0.0	0.0	0.0	0.0
3	0.0012539491561235666	0.0	0.0	0.0	0.0
4	0.0012539491561235666	0.0	0.0	0.0	0.0
5	0.0012539491561235666	0.0	0.0	0.0	0.0
6	0.0012539491561235666	0.0	0.0	0.0	0.0
7	0.0012539491561235666	0.0	0.0	0.0	0.0
8	0.0012539491561235666	0.0	0.0	0.0	0.0
9	0.0012539491561235666	0.0	0.0	0.0	0.0
10-11	0.0012539491561235666	0.0	0.0	0.0	0.0
12-13	0.001316646613929745	0.0	0.0	0.0	0.0
14-15	0.0014420415295421018	0.0	0.0	0.0	0.0
16-17	0.0015674364451544584	0.0	0.0	0.0	0.0
18-19	0.0015987851740575476	0.0	0.0	0.0	0.0
20-21	0.0017555288185729934	0.0	0.0	0.0	0.0
22-23	0.0019122724630884391	0.0	0.0	0.0	0.0
24-25	0.0019436211919915283	0.0	0.0	0.0	0.0
26-27	0.0019749699208946175	0.0	0.0	0.0	0.0
28-29	0.002069016107603885	0.0	0.0	0.0	0.0
30-31	0.00225710848102242	0.0	0.0	0.0	0.0
32-33	0.0023511546677316875	0.0	0.0	0.0	0.0
34-35	0.0029154317879872926	0.0	0.0	0.0	0.0
36-37	0.0040439860284985025	0.0	0.0	0.0	0.0
38-39	0.0063324432384240115	0.0	0.0	0.0	6.269745780617833E-5
40-41	0.009561362315442197	0.0	0.0	0.0	6.269745780617833E-5
42-43	0.014044230548583947	0.0	0.0	0.0	6.269745780617833E-5
44-45	0.018150914034888627	0.0	0.0	0.0	6.269745780617833E-5
46-47	0.022821874641448914	0.0	0.0	0.0	6.269745780617833E-5
48-49	0.027649578892524646	0.0	0.0	0.0	6.269745780617833E-5
50-51	0.03438955560668881	0.0	0.0	0.0	6.269745780617833E-5
52-53	0.04332394334406923	0.0	0.0	0.0	6.269745780617833E-5
54-55	0.05586343490530489	0.0	0.0	0.0	6.269745780617833E-5
56-57	0.07044059384524136	0.0	0.0	0.0	6.269745780617833E-5
58-59	0.08495505532737163	0.0	0.0	0.0	6.269745780617833E-5
60-61	0.10370159521141896	0.0	0.0	0.0	6.269745780617833E-5
62-63	0.12900001943621192	0.0	0.0	0.0	6.269745780617833E-5
64-65	0.15611666993738404	0.0	0.0	0.0	6.269745780617833E-5
66-67	0.18969115859259256	0.0	0.0	0.0	6.269745780617833E-5
68-69	0.23000562396196522	0.0	0.0	0.0	6.269745780617833E-5
70-71	0.2814175393630315	0.0	0.0	0.0	6.269745780617833E-5
72-73	0.33934999037594027	0.0	0.0	0.0	6.269745780617833E-5
74-75	0.4023609354711495	0.0	0.0	0.0	6.269745780617833E-5
76-77	0.4681305687098305	0.0	0.0	0.0	6.269745780617833E-5
78-79	0.5489789405508976	0.0	0.0	0.0	9.404618670926749E-5
80-81	0.6453449331989936	0.0	0.0	0.0	1.2539491561235666E-4
82-83	0.7634355949769305	0.0	0.0	0.0	1.2539491561235666E-4
84-85	0.906354450046114	0.0	0.0	0.0	1.2539491561235666E-4
86-87	1.067048034403349	0.0	0.0	0.0	1.2539491561235666E-4
88	1.1997158551212224	0.0	0.0	0.0	1.2539491561235666E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2715	0.0	88.25237	1
AACGTGT	1370	0.0	84.87012	1
CGTGTGG	1575	0.0	79.688545	3
ACGTGTG	1555	0.0	78.602325	2
TCGAGTG	1055	0.0	74.85991	2
AACGAGT	44170	0.0	73.109726	1
ATCGAGT	1050	0.0	73.07633	1
GTGGAAT	52345	0.0	73.06358	6
ACGAGTG	47975	0.0	72.413895	2
TGGAATC	52855	0.0	72.33608	7
CGAGTGG	49755	0.0	71.94463	3
AGCGAGT	465	0.0	71.875984	1
GAATCAC	53475	0.0	71.47104	9
AGTGGAA	51635	0.0	71.327995	5
GGAATCA	53625	0.0	71.23606	8
GAGTGGA	51765	0.0	71.203354	4
AATGAGT	1560	0.0	65.178986	1
ATGAGTG	1760	0.0	61.96804	2
TGAGTGG	1870	0.0	61.838467	3
GTGTGGA	2350	0.0	58.209026	4
>>END_MODULE