FastQCFastQC Report
Thu 26 May 2016
SRR2132785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737160
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29147410.648774642330006No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG406081.4835815224539304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235100.8589194639699543No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228220.8337839220213652No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG178250.6512224349325578No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTACTT101050.36917827236990164No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97550.3563912960879159No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82430.3011515585497377No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68610.2506612693448684No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68220.24923643484487573No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAAAAAAAAAAAAAAAAAAAA66510.242989083575677No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTAACGAGTGGAATCACTGCTG53930.1970290373964255No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.14610033757617383No Hit
CACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACT39570.14456590042233555No Hit
ACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTC35950.13134051352496748No Hit
CTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCA34350.12549503865320258No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAAAAAAAAAAAAAAAAAA31440.11486358123018019No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC31000.11325607564044483No Hit
TACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG30800.11252539128147424No Hit
CAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGC29630.10825088778149616No Hit
TACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACT29580.10806821669175351No Hit
CCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCAC29430.10752020342252554No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28990.10591269783279018No Hit
AGCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27380.10003068874307676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT57850.084.6338041
ATCGAGT19350.079.070821
TCGAGTG22150.073.005472
AACGTGT29150.069.122451
CGTGTGG32600.069.0660863
ACGTGTG31050.068.127372
AATGAGT31300.060.4638251
ATGAGTG32600.060.129722
GCGAGTG10800.057.454082
TGAGTGG36650.056.432023
AGCGAGT10900.054.4199261
GTGTGGA43800.054.1946264
GTGGAAT1526650.048.945716
TGGAATC1528250.048.8724447
TGTGGAA49200.048.3419725
GGAATCA1569300.047.6123858
AGTGGAA1525850.047.5420045
GAGTGGA1530800.047.4527554
GAATCAC1576550.047.3695839
CGAGTGG1497600.047.303493
Produced by FastQC (version 0.11.5)