##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132785_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2737160
Sequences flagged as poor quality	0
Sequence length	100
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.067132356164784	31.0	25.0	33.0	16.0	34.0
2	28.290969472007482	31.0	26.0	34.0	16.0	34.0
3	29.320121951219512	31.0	28.0	34.0	16.0	34.0
4	34.80458614037908	35.0	35.0	37.0	32.0	37.0
5	33.809053179207645	35.0	35.0	37.0	28.0	37.0
6	35.263878253372106	37.0	35.0	37.0	32.0	37.0
7	35.59725481886335	37.0	35.0	37.0	33.0	37.0
8	35.90853622002368	37.0	35.0	37.0	35.0	37.0
9	37.87558125940756	39.0	38.0	39.0	35.0	39.0
10-11	37.87751757295884	39.0	38.0	39.0	35.0	39.0
12-13	37.888267401248015	39.0	38.0	39.0	35.0	39.0
14-15	39.35287414692601	41.0	39.0	41.0	36.0	41.0
16-17	39.33995254205088	41.0	39.0	41.0	36.0	41.0
18-19	39.28980823188999	41.0	39.0	41.0	36.0	41.0
20-21	39.20724418740592	40.5	39.0	41.0	36.0	41.0
22-23	39.04097568282454	40.0	39.0	41.0	35.5	41.0
24-25	38.90139578979672	40.0	39.0	41.0	35.0	41.0
26-27	38.59151437986819	40.0	38.5	41.0	35.0	41.0
28-29	38.24725755892969	40.0	38.0	41.0	34.0	41.0
30-31	37.896893860790016	40.0	38.0	41.0	33.5	41.0
32-33	37.75079790731999	40.0	38.0	41.0	33.0	41.0
34-35	37.593217787780034	40.0	37.0	41.0	33.0	41.0
36-37	37.229676745239594	39.5	37.0	41.0	33.0	41.0
38-39	36.42403768869924	39.0	35.5	41.0	31.5	41.0
40-41	35.95254533896447	39.0	35.0	41.0	30.0	41.0
42-43	35.628137193295245	38.5	35.0	41.0	29.5	41.0
44-45	35.25400067953645	38.0	35.0	41.0	27.5	41.0
46-47	34.92178444080726	38.0	35.0	41.0	25.5	41.0
48-49	34.68175243683234	38.0	35.0	41.0	24.0	41.0
50-51	34.23640744421225	38.0	35.0	40.5	21.5	41.0
52-53	33.51428067778281	37.5	33.5	40.0	11.0	41.0
54-55	32.410267211270074	37.0	33.0	40.0	2.0	41.0
56-57	31.452759794823834	37.0	32.5	40.0	2.0	41.0
58-59	30.91724798696459	36.5	31.0	40.0	2.0	41.0
60-61	30.51817175466542	36.0	30.5	40.0	2.0	41.0
62-63	30.107478006400797	35.5	29.5	40.0	2.0	41.0
64-65	29.722276374051937	35.0	29.0	40.0	2.0	41.0
66-67	29.280318834120038	35.0	28.0	39.0	2.0	41.0
68-69	28.85300457408409	35.0	26.5	39.0	2.0	41.0
70-71	28.45830441041079	35.0	26.0	38.5	2.0	40.0
72-73	28.04035989858101	35.0	25.0	37.0	2.0	40.0
74-75	27.565949743529792	35.0	23.5	37.0	2.0	39.0
76-77	27.103801202706457	35.0	20.0	36.0	2.0	39.0
78-79	26.71281090619474	35.0	18.0	36.0	2.0	38.5
80-81	26.34371026903798	34.5	11.5	35.0	2.0	37.0
82-83	25.98722270528577	34.0	6.0	35.0	2.0	37.0
84-85	25.64920592877289	34.0	2.0	35.0	2.0	36.5
86-87	25.350069049671923	34.0	2.0	35.0	2.0	36.0
88-89	25.053685937248826	34.0	2.0	35.0	2.0	36.0
90-91	24.73914659720294	34.0	2.0	35.0	2.0	35.5
92-93	24.44314344064651	34.0	2.0	35.0	2.0	35.0
94-95	24.15495385728273	34.0	2.0	35.0	2.0	35.0
96-97	23.818590984816378	33.5	2.0	35.0	2.0	35.0
98-99	23.481129345745224	33.0	2.0	35.0	2.0	35.0
100	23.307205278463808	33.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.15578141672636292
1101	2	2.191701551799291
1101	3	1.1416542538175385
1101	4	0.6092907110487431
1101	5	2.2276517888875844
1101	6	0.9920783465401897
1101	7	0.5408433740849858
1101	8	0.4049932373438594
1101	9	0.3275323367370433
1101	10-11	0.42068005588342317
1101	12-13	0.4234957692930976
1101	14-15	0.4929396537554638
1101	16-17	0.4658322744183039
1101	18-19	0.39198637687903215
1101	20-21	0.3989311226457417
1101	22-23	0.34811350507366257
1101	24-25	0.3334575128928776
1101	26-27	0.3099507770463106
1101	28-29	0.3872793800655714
1101	30-31	0.4800322409516653
1101	32-33	0.40595432181657287
1101	34-35	0.37892151311783095
1101	36-37	0.336833960835925
1101	38-39	0.21078194107928283
1101	40-41	0.21797751522944253
1101	42-43	0.25346557961375993
1101	44-45	0.31124820803228204
1101	46-47	0.30759653782917695
1101	48-49	0.2349064049329712
1101	50-51	0.2230828181832223
1101	52-53	0.3810636519260626
1101	54-55	0.3424617218001629
1101	56-57	0.12301268200028304
1101	58-59	0.09347399130054868
1101	60-61	0.20538223394077804
1101	62-63	0.24478231180423649
1101	64-65	0.29188561010030156
1101	66-67	0.22794084630732314
1101	68-69	0.32401011787057143
1101	70-71	0.2612863646146941
1101	72-73	0.34359076935247757
1101	74-75	0.3992577237567936
1101	76-77	0.39086933170341354
1101	78-79	0.467722103878625
1101	80-81	0.42427668087126946
1101	82-83	0.5280171630708175
1101	84-85	0.5120499244162531
1101	86-87	0.540115561848495
1101	88-89	0.4583471299712585
1101	90-91	0.4410435556078909
1101	92-93	0.5162352778614476
1101	94-95	0.473539968796036
1101	96-97	0.3910541480819738
1101	98-99	0.46819458839453887
1101	100	0.39882894557250026
1102	1	1.3383455545916334
1102	2	2.919078754669446
1102	3	3.0943988072617863
1102	4	1.3233071874101086
1102	5	2.5716719455145736
1102	6	1.0866634951512921
1102	7	0.6613768206403279
1102	8	0.5042163875562835
1102	9	0.3611692524226342
1102	10-11	0.42677873385687093
1102	12-13	0.42270223136892326
1102	14-15	0.5540618117410361
1102	16-17	0.4343883728743876
1102	18-19	0.4176773348812759
1102	20-21	0.4058643364547194
1102	22-23	0.34301549607052806
1102	24-25	0.3988269409696841
1102	26-27	0.49209384133494893
1102	28-29	0.49801853138352925
1102	30-31	0.5165832076767387
1102	32-33	0.47022686870693065
1102	34-35	0.49087832046367197
1102	36-37	0.5491046847253926
1102	38-39	0.6057239547067894
1102	40-41	0.6198592703384165
1102	42-43	0.7072994803299721
1102	44-45	0.7689908171073725
1102	46-47	0.729835652303187
1102	48-49	0.6314566512919768
1102	50-51	0.6781929691731889
1102	52-53	0.5954194898531924
1102	54-55	0.7179198715244368
1102	56-57	0.6697802301899856
1102	58-59	0.6841147744643763
1102	60-61	0.7372982693926922
1102	62-63	0.8576417956299522
1102	64-65	0.792831167970153
1102	66-67	0.7491052891619638
1102	68-69	0.7676986480946795
1102	70-71	0.804777872654622
1102	72-73	0.7899993265170338
1102	74-75	0.7600881334840857
1102	76-77	0.8316106823051648
1102	78-79	0.8430842735964568
1102	80-81	0.8909418699962615
1102	82-83	0.9125813643035308
1102	84-85	0.9359967347735925
1102	86-87	0.8869661740254884
1102	88-89	0.8755441205749186
1102	90-91	0.9333720697816617
1102	92-93	1.0036175965691498
1102	94-95	1.0109763510804761
1102	96-97	1.0153568481749993
1102	98-99	1.0143395823973336
1102	100	1.0097953011745027
1103	1	1.291582993882976
1103	2	2.929820228086644
1103	3	3.1203964735913097
1103	4	1.363917110206856
1103	5	2.5927375924714084
1103	6	1.1898594188817313
1103	7	0.7280029261557956
1103	8	0.5050947808422706
1103	9	0.40528407038256375
1103	10-11	0.4683237595348686
1103	12-13	0.47124852075263846
1103	14-15	0.5286842426361815
1103	16-17	0.4385375072029234
1103	18-19	0.4252712744015028
1103	20-21	0.41246613555149736
1103	22-23	0.3968929919423019
1103	24-25	0.4514884347861994
1103	26-27	0.5277583746142298
1103	28-29	0.5618355190253297
1103	30-31	0.5858119506222863
1103	32-33	0.5828611560446646
1103	34-35	0.6177211111226697
1103	36-37	0.5450425067913685
1103	38-39	0.6110041276044598
1103	40-41	0.6937936231293094
1103	42-43	0.6773613548373945
1103	44-45	0.7302457015618202
1103	46-47	0.727713911231838
1103	48-49	0.7865575961374063
1103	50-51	0.810233971219624
1103	52-53	0.7165336275502483
1103	54-55	0.7033457325831947
1103	56-57	0.6629671575788478
1103	58-59	0.69491772532092
1103	60-61	0.6916480542771559
1103	62-63	0.6416470308741182
1103	64-65	0.6436364467454929
1103	66-67	0.557207586813778
1103	68-69	0.5561952176939258
1103	70-71	0.5796740282536419
1103	72-73	0.6484693308999177
1103	74-75	0.6614794307508909
1103	76-77	0.6406244202046203
1103	78-79	0.6826271730682549
1103	80-81	0.6683629900521382
1103	82-83	0.6776681221640004
1103	84-85	0.6943263223641587
1103	86-87	0.7091562400684559
1103	88-89	0.6844840199577682
1103	90-91	0.6580891890512746
1103	92-93	0.6668791228069963
1103	94-95	0.6793091227836392
1103	96-97	0.643815408218483
1103	98-99	0.702654590475678
1103	100	0.7405594342121873
1104	1	5.926482388687489
1104	2	4.904084656475103
1104	3	3.994540938350159
1104	4	1.800596258826836
1104	5	2.8216048307659136
1104	6	1.2500014296168658
1104	7	0.6970955239989891
1104	8	0.5333775019732769
1104	9	0.43222274912054814
1104	10-11	0.4527927713748028
1104	12-13	0.3936238641693066
1104	14-15	0.5326189665829517
1104	16-17	0.43329007610623194
1104	18-19	0.38858721284560005
1104	20-21	0.3673268868151709
1104	22-23	0.3655409079775396
1104	24-25	0.3516736842460233
1104	26-27	0.42490197899823556
1104	28-29	0.41806064881482996
1104	30-31	0.44251774153164547
1104	32-33	0.44154189831350976
1104	34-35	0.4918240970144794
1104	36-37	0.5192508641597939
1104	38-39	0.6360006323316298
1104	40-41	0.5409264972701635
1104	42-43	0.6149143216640738
1104	44-45	0.6889437002125973
1104	46-47	0.6046782545605041
1104	48-49	0.7498056089648131
1104	50-51	0.8109724025998446
1104	52-53	0.6935711516260739
1104	54-55	0.734474241299381
1104	56-57	0.8330318812323085
1104	58-59	0.9160207894286252
1104	60-61	0.8693619747511434
1104	62-63	0.9224702042885369
1104	64-65	0.9439628270116245
1104	66-67	0.9406874364437172
1104	68-69	0.9047958864398318
1104	70-71	0.8841096516832145
1104	72-73	0.8867579426655468
1104	74-75	0.8874651954579242
1104	76-77	0.8911362210278391
1104	78-79	0.9080307915311145
1104	80-81	0.9336176072342148
1104	82-83	0.9557827524472415
1104	84-85	0.9498726115087699
1104	86-87	0.8742611960231272
1104	88-89	0.785731734587074
1104	90-91	0.787538695802283
1104	92-93	0.8033907916409007
1104	94-95	0.8156753833794497
1104	96-97	0.8174438924921965
1104	98-99	0.8368511421323888
1104	100	0.8018114262732752
1105	1	1.355626831750758
1105	2	2.9507686621740383
1105	3	3.1996320160793807
1105	4	1.3513848341894743
1105	5	2.5863826584783993
1105	6	1.1842640107740507
1105	7	0.7311862288115307
1105	8	0.5099001494905124
1105	9	0.36499433286903127
1105	10-11	0.4611406070237223
1105	12-13	0.4035757435737821
1105	14-15	0.46734231499232237
1105	16-17	0.40622457185594385
1105	18-19	0.3599618650290708
1105	20-21	0.3307106515525504
1105	22-23	0.3125912565112188
1105	24-25	0.3556839968266985
1105	26-27	0.3809432771307826
1105	28-29	0.4421251299347304
1105	30-31	0.4454941425987613
1105	32-33	0.4503319749048629
1105	34-35	0.5375840581928983
1105	36-37	0.5524212775235995
1105	38-39	0.49623894043730843
1105	40-41	0.5327524742640719
1105	42-43	0.5402032842863491
1105	44-45	0.6496144721501409
1105	46-47	0.72948782800119
1105	48-49	0.6164786184024749
1105	50-51	0.6381276459040848
1105	52-53	0.6569213299567451
1105	54-55	0.6450808046330678
1105	56-57	0.6964306821617399
1105	58-59	0.6051909780862204
1105	60-61	0.575776070045702
1105	62-63	0.6344745358592796
1105	64-65	0.5772429400340613
1105	66-67	0.6410827998462487
1105	68-69	0.5925286007952124
1105	70-71	0.6109866178625367
1105	72-73	0.553679778371837
1105	74-75	0.5395286677903748
1105	76-77	0.5298387817224217
1105	78-79	0.5127509844915998
1105	80-81	0.45862924892821155
1105	82-83	0.43697826119166194
1105	84-85	0.3709813215038942
1105	86-87	0.4586589355742916
1105	88-89	0.4400478388507558
1105	90-91	0.4518780595490419
1105	92-93	0.5030202654458904
1105	94-95	0.513685275233609
1105	96-97	0.4754850758811493
1105	98-99	0.5127051930594213
1105	100	0.5205995904713596
1106	1	-0.823701701711375
1106	2	1.8302973332692396
1106	3	2.645844163673811
1106	4	1.1379987881989848
1106	5	2.390267556201813
1106	6	1.0688871588692805
1106	7	0.5917408132304516
1106	8	0.43437802580773877
1106	9	0.2997210653669029
1106	10-11	0.3970801694141599
1106	12-13	0.3182250016384387
1106	14-15	0.36685491659418545
1106	16-17	0.31383804437683693
1106	18-19	0.27206378996151415
1106	20-21	0.2904616619405189
1106	22-23	0.3253454054706211
1106	24-25	0.3428251846575989
1106	26-27	0.24618948928783624
1106	28-29	0.3425068809483278
1106	30-31	0.36270407693537265
1106	32-33	0.39255963385193837
1106	34-35	0.39817502869149024
1106	36-37	0.36794763883614223
1106	38-39	0.35687805506604775
1106	40-41	0.36179905582736893
1106	42-43	0.30976839634790565
1106	44-45	0.4458886227599308
1106	46-47	0.39639456077917146
1106	48-49	0.3771069260343296
1106	50-51	0.3327951594658174
1106	52-53	0.37804507215290073
1106	54-55	0.2683380817543721
1106	56-57	0.24004415392557021
1106	58-59	0.3949951963731735
1106	60-61	0.4227380878993863
1106	62-63	0.43727788220517283
1106	64-65	0.4736522680054094
1106	66-67	0.5300970001644387
1106	68-69	0.49790839860027347
1106	70-71	0.6579411266514086
1106	72-73	0.6851224566219898
1106	74-75	0.5538696330842043
1106	76-77	0.5798107327392827
1106	78-79	0.6478405273688779
1106	80-81	0.659269781147259
1106	82-83	0.6369123640614021
1106	84-85	0.6788816289449748
1106	86-87	0.6410575740819482
1106	88-89	0.6454140663987893
1106	90-91	0.6759161185366978
1106	92-93	0.6576927822118783
1106	94-95	0.678457318573642
1106	96-97	0.7233765049295222
1106	98-99	0.7044764882373045
1106	100	0.7510335320878347
1107	1	-0.6983256802510169
1107	2	1.8779311116259088
1107	3	-0.6113580040705244
1107	4	0.06137181699369876
1107	5	1.910085050115697
1107	6	0.7900308068201554
1107	7	0.34603598620032017
1107	8	0.3266579563406822
1107	9	0.29736375969634565
1107	10-11	0.34202113016837643
1107	12-13	0.32234301810584753
1107	14-15	0.42696679266990856
1107	16-17	0.33735392256138397
1107	18-19	0.32657585366032293
1107	20-21	0.2871613952366303
1107	22-23	0.29315254135004665
1107	24-25	0.25481019031730767
1107	26-27	0.3092155316813461
1107	28-29	0.30621673968830976
1107	30-31	0.3281963549276483
1107	32-33	0.35484487520344743
1107	34-35	0.4657586837751495
1107	36-37	0.44440656333723183
1107	38-39	0.42609953558270774
1107	40-41	0.4030769085510002
1107	42-43	0.43272082381839994
1107	44-45	0.533853610122506
1107	46-47	0.5190946274551393
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2216	94-95	-0.07931525298335984
2216	96-97	-0.10851944579254891
2216	98-99	-0.1428217684779085
2216	100	-0.23313790528605693
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1579.0
3	368.0
4	394.0
5	746.0
6	1647.0
7	2658.0
8	3810.0
9	5663.0
10	8495.0
11	12258.0
12	13158.0
13	17838.0
14	19521.0
15	22349.0
16	24608.0
17	26576.0
18	33359.0
19	50206.0
20	86122.0
21	100641.0
22	57327.0
23	42726.0
24	41780.0
25	42146.0
26	43798.0
27	47577.0
28	53162.0
29	58490.0
30	56078.0
31	59349.0
32	68032.0
33	84466.0
34	113706.0
35	185159.0
36	291751.0
37	442954.0
38	495121.0
39	121039.0
40	503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.084638013279292	48.37305084565943	21.653827481891792	17.88848365916949
2	12.42120539082455	49.35851789226316	21.96702180947826	16.25325490743403
3	15.975899855283679	21.132126621116893	16.502373515707806	46.38960000789162
4	42.30684358970612	23.206498706688684	18.24248491136799	16.24417279223721
5	15.580862191484037	47.703715070174766	19.09009369422691	17.625329044114288
6	46.20529841976954	20.011319723357364	20.18023864227628	13.603143214596816
7	16.54473606140275	20.13542919524339	47.83966539474972	15.48016934860413
8	45.473775738356544	20.49405222931798	19.362843238977625	14.66932879334785
9	43.982960440748805	21.75232722968332	20.73886802379108	13.52584430577679
10-11	13.761964956378145	51.2189093805258	20.242093995235937	14.777031667860118
12-13	13.359211737713542	21.410111210159435	34.920501541743995	30.310175510383026
14-15	13.926387204255505	35.87112189276476	20.011380408890965	30.191110494088765
16-17	28.899059609230004	20.9280239372196	35.01830364319221	15.15461281035818
18-19	14.08200470560727	21.31599906472402	35.64605284309284	28.955943386575868
20-21	28.718343100147596	36.57303555510091	21.049153136827954	13.659468207923542
22-23	27.526469040903713	22.097630390623856	36.145000657615924	14.23089991085651
24-25	12.870548305542972	38.991436379312866	21.043837408116442	27.09417790702772
26-27	15.715488995069524	26.66578253843081	41.00201321844781	16.616715248051857
28-29	24.91052770024405	24.423234301246545	36.93021233687472	13.736025661634688
30-31	21.68976238144646	24.713151587777112	39.40745151909278	14.189634511683643
32-33	17.103055721989215	28.83307881161496	38.09048429759312	15.9733811688027
34-35	15.422627833228603	28.66719519501966	40.00401876397434	15.906158207777404
36-37	15.55880913063175	28.461726753277116	38.41260649724532	17.56685761884581
38-39	16.56072352365225	26.3880628096275	39.26779947098453	17.783414195735723
40-41	17.032654284002398	28.24431162226541	37.11659164973914	17.606442443993046
42-43	16.94993091323857	27.19607122476105	39.422729142630004	16.43126871937038
44-45	16.945465372868227	29.416183197182484	36.89150433295825	16.74684709699104
46-47	16.187489953090065	28.941201829633634	38.440957050373385	16.43035116690292
48-49	15.551118677753584	28.62468763243654	37.075344517675255	18.748849172134623
50-51	17.790739306434407	29.544381767963873	36.15959607768636	16.505282847915357
52-53	18.064486665899445	28.071537782843865	37.29326618331808	16.57070936793861
54-55	19.11574405588274	29.835139341507254	33.447131333206684	17.601985269403322
56-57	17.527090122608836	32.77808019991524	31.6447339578249	18.050095719651026
58-59	18.773582837685776	30.83719987139955	31.225485539756537	19.163731751158135
60-61	18.592793260167472	32.196126642213095	29.780045740840873	19.43103435677856
62-63	19.11490994395096	31.592457354922892	30.188518632383644	19.104114068742504
64-65	18.163205658419677	31.285584328281868	31.187106343801606	19.364103669496853
66-67	19.245221324292334	32.018753014072985	30.160805360300458	18.57522030133423
68-69	19.417918572535037	30.63079980709933	31.311962033640704	18.639319586724927
70-71	17.224769469084745	33.555564892077925	29.999981732891023	19.219683905946308
72-73	18.174702281352705	30.89142421235534	30.83554488102214	20.098328625269808
74-75	18.615208744573277	32.01541092574792	30.030955567270905	19.338424762407893
76-77	19.052523360115508	30.8158796175868	31.123115919367052	19.008481102930645
78-79	18.639861388544094	31.911268939653414	29.695257562845995	19.753612108956496
80-81	18.239164205453008	32.35857824905777	30.176805502986337	19.225452042502884
82-83	18.210363332993563	32.272094393728324	29.318874398046713	20.1986678752314
84-85	18.11703555427035	31.839570381409253	29.487467783263867	20.555926281056532
86-87	18.831788726924522	31.988989313372002	28.793230499214694	20.38599146048878
88-89	19.670132546142717	31.42216750208245	29.349818790278974	19.557881161495857
90-91	19.02305674494732	32.632290403191625	28.579293866635492	19.76535898522556
92-93	17.756945154832017	32.91530272252992	29.001903432755118	20.325848689882946
94-95	17.86515103162641	32.81393825019633	28.235211808157985	21.085698910019275
96-97	18.634888298812914	32.179579734056624	28.476093885406726	20.709438081723736
98-99	19.261923298799733	31.52614351982598	28.400327202727226	20.811605978647062
100	19.188538485145187	32.460798784141225	27.503616887576904	20.847045843136684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20315.0
1	13019.0
2	4589.5
3	3190.0
4	3086.5
5	3361.0
6	3744.0
7	4285.0
8	4385.5
9	4337.0
10	4687.5
11	7193.5
12	29749.5
13	33165.0
14	15117.0
15	16445.5
16	18490.0
17	17748.5
18	18355.0
19	20145.5
20	22159.0
21	24612.5
22	27603.5
23	31585.0
24	35362.5
25	38384.5
26	42109.5
27	46129.5
28	48986.5
29	51568.0
30	55122.0
31	59417.0
32	64604.5
33	70355.0
34	77892.0
35	82847.5
36	83146.5
37	83087.0
38	82035.0
39	79432.5
40	76474.0
41	74278.0
42	72000.0
43	69765.5
44	69176.5
45	70241.0
46	72585.0
47	88000.5
48	119135.5
49	121525.5
50	101318.0
51	80266.0
52	56250.0
53	45788.0
54	42731.5
55	41647.5
56	37717.5
57	31642.5
58	27297.0
59	22007.5
60	17185.5
61	14117.0
62	11447.5
63	8558.5
64	6162.0
65	4734.5
66	3493.5
67	2623.5
68	2001.5
69	1457.5
70	1051.0
71	843.5
72	671.0
73	452.0
74	304.0
75	200.5
76	128.5
77	84.5
78	49.0
79	29.0
80	14.0
81	6.5
82	3.5
83	1.5
84	0.0
85	0.0
86	0.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13630916716596767
2	0.005078256294845753
3	0.002959271653830978
4	0.0
5	0.0023747241666544888
6	0.012567770974294526
7	0.0030688743076765696
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.8267108974265297E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.037527948676731E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.8267108974265295E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.2786976281985708E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.3841061538236716E-4
74-75	4.0187639743383656E-4
76-77	5.845474871764895E-4
78-79	4.566777243566324E-4
80-81	0.0010229581025588566
82-83	0.0010594923205073871
84-85	0.0012604305192243054
86-87	3.6534217948530594E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.4801326922795895E-5
96-97	0.0013152318461471014
98-99	6.941501410220813E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2737160.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.079083820404264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.70331192366523	49.05152008308165
2	5.427927492678716	5.87074691695298
3	1.3404425486105687	2.1746971482824176
4	0.620737975604791	1.342757640529582
5	0.35411196010413903	0.9575025186139692
6	0.23901719968458207	0.7755498705756491
7	0.1635483396203984	0.6191181056913628
8	0.1278397986270609	0.5530767348429143
9	0.10127644381459795	0.4929243564673895
>10	0.8134243431182924	8.079108730375914
>50	0.055567344552088005	2.088374585017988
>100	0.04180613392133891	4.84489340718677
>500	0.006104719999079718	2.288404244636054
>1k	0.004069813332719674	4.351911375801393
>5k	4.069813332719675E-4	1.6039188201764674
>10k+	4.069813332719675E-4	14.905495461767556
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	291474	10.648774642330006	No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG	40608	1.4835815224539304	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23510	0.8589194639699543	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22822	0.8337839220213652	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	17825	0.6512224349325578	No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTACTT	10105	0.36917827236990164	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9755	0.3563912960879159	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8243	0.3011515585497377	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6861	0.2506612693448684	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6822	0.24923643484487573	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	6651	0.242989083575677	No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTAACGAGTGGAATCACTGCTG	5393	0.1970290373964255	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3999	0.14610033757617383	No Hit
CACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACT	3957	0.14456590042233555	No Hit
ACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTC	3595	0.13134051352496748	No Hit
CTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCA	3435	0.12549503865320258	No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAAAAAAAAAAAAAAAAAA	3144	0.11486358123018019	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3100	0.11325607564044483	No Hit
TACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG	3080	0.11252539128147424	No Hit
CAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGC	2963	0.10825088778149616	No Hit
TACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACT	2958	0.10806821669175351	No Hit
CCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCAC	2943	0.10752020342252554	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2899	0.10591269783279018	No Hit
AGCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2738	0.10003068874307676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010594923205073873	3.6534217948530594E-5	0.0	0.0	0.0
2	0.0010960265384559178	3.6534217948530594E-5	0.0	0.0	0.0
3	0.0010960265384559178	3.6534217948530594E-5	0.0	0.0	0.0
4	0.0010960265384559178	3.6534217948530594E-5	0.0	3.6534217948530594E-5	0.0
5	0.0010960265384559178	3.6534217948530594E-5	0.0	3.6534217948530594E-5	0.0
6	0.0010960265384559178	3.6534217948530594E-5	0.0	3.6534217948530594E-5	0.0
7	0.0010960265384559178	3.6534217948530594E-5	0.0	3.6534217948530594E-5	0.0
8	0.0011325607564044483	3.6534217948530594E-5	0.0	3.6534217948530594E-5	0.0
9	0.0012421634102500402	3.6534217948530594E-5	0.0	3.6534217948530594E-5	0.0
10-11	0.0014979029358897543	3.6534217948530594E-5	0.0	3.6534217948530594E-5	0.0
12-13	0.0018084437884522643	5.480132692279589E-5	0.0	3.6534217948530594E-5	0.0
14-15	0.002082450423066244	7.306843589706119E-5	0.0	5.480132692279589E-5	0.0
16-17	0.0022103201858861008	7.306843589706119E-5	0.0	7.306843589706119E-5	0.0
18-19	0.0023747241666544888	7.306843589706119E-5	0.0	9.133554487132648E-5	0.0
20-21	0.002740066346139794	1.0960265384559178E-4	0.0	1.6440398076838768E-4	0.0
22-23	0.0031236756345993655	1.6440398076838768E-4	0.0	1.8267108974265298E-4	0.0
24-25	0.0036351546858787937	1.8267108974265298E-4	0.0	1.8267108974265298E-4	0.0
26-27	0.0042562363910038144	2.5573952563971415E-4	0.0	2.1920530769118355E-4	0.0
28-29	0.004712914115360446	2.5573952563971415E-4	0.0	2.1920530769118355E-4	0.0
30-31	0.005425331365356793	2.5573952563971415E-4	0.0	2.1920530769118355E-4	0.0
32-33	0.00675883032047816	2.5573952563971415E-4	0.0	2.1920530769118355E-4	0.0
34-35	0.008530739890981895	2.5573952563971415E-4	0.0	2.3747241666544885E-4	0.0
36-37	0.011855353724298176	2.5573952563971415E-4	0.0	2.5573952563971415E-4	0.0
38-39	0.017098013999912315	2.5573952563971415E-4	0.0	2.5573952563971415E-4	0.0
40-41	0.02416738517295299	2.5573952563971415E-4	0.0	2.5573952563971415E-4	0.0
42-43	0.03384895292931359	2.5573952563971415E-4	0.0	2.9227374358824476E-4	0.0
44-45	0.045430300018997796	2.7400663461397945E-4	0.0	2.9227374358824476E-4	0.0
46-47	0.05944117260225928	3.2880796153677536E-4	0.0	3.2880796153677536E-4	0.0
48-49	0.07558929693550981	3.6534217948530596E-4	0.0	4.018763974338365E-4	0.0
50-51	0.09402080989054348	3.6534217948530596E-4	0.0	4.384106153823671E-4	0.0
52-53	0.11585000511479052	4.384106153823671E-4	0.0	4.384106153823671E-4	0.0
54-55	0.14480337283900102	4.932119423051631E-4	0.0	4.566777243566324E-4	0.0
56-57	0.17973008519779626	5.297461602536936E-4	0.0	4.749448333308977E-4	0.0
58-59	0.2144558593578746	5.480132692279589E-4	0.0	4.749448333308977E-4	0.0
60-61	0.2533648014730597	5.480132692279589E-4	0.0	4.749448333308977E-4	0.0
62-63	0.2944109953382338	5.480132692279589E-4	0.0	4.749448333308977E-4	0.0
64-65	0.33768577649826825	5.480132692279589E-4	0.0	4.749448333308977E-4	0.0
66-67	0.3916102821902995	5.480132692279589E-4	0.0	4.749448333308977E-4	0.0
68-69	0.45360885004895585	5.480132692279589E-4	0.0	4.749448333308977E-4	0.0
70-71	0.53294290432419	5.845474871764895E-4	0.0	4.749448333308977E-4	0.0
72-73	0.614469011676336	5.845474871764895E-4	0.0	4.749448333308977E-4	0.0
74-75	0.6979496996887284	6.210817051250201E-4	0.0	4.749448333308977E-4	0.0
76-77	0.793559748060033	6.210817051250201E-4	0.0	4.749448333308977E-4	0.0
78-79	0.90738210407868	6.210817051250201E-4	0.0	4.749448333308977E-4	0.0
80-81	1.029790001315232	6.941501410220813E-4	0.0	5.114790512794283E-4	0.0
82-83	1.1692045770068247	6.941501410220813E-4	0.0	5.114790512794283E-4	0.0
84-85	1.3192871443393883	6.941501410220813E-4	0.0	5.114790512794283E-4	0.0
86-87	1.480530915255228	6.941501410220813E-4	0.0	5.114790512794283E-4	0.0
88	1.6118896958891697	6.941501410220813E-4	0.0	5.114790512794283E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5785	0.0	84.633804	1
ATCGAGT	1935	0.0	79.07082	1
TCGAGTG	2215	0.0	73.00547	2
AACGTGT	2915	0.0	69.12245	1
CGTGTGG	3260	0.0	69.066086	3
ACGTGTG	3105	0.0	68.12737	2
AATGAGT	3130	0.0	60.463825	1
ATGAGTG	3260	0.0	60.12972	2
GCGAGTG	1080	0.0	57.45408	2
TGAGTGG	3665	0.0	56.43202	3
AGCGAGT	1090	0.0	54.419926	1
GTGTGGA	4380	0.0	54.194626	4
GTGGAAT	152665	0.0	48.94571	6
TGGAATC	152825	0.0	48.872444	7
TGTGGAA	4920	0.0	48.341972	5
GGAATCA	156930	0.0	47.612385	8
AGTGGAA	152585	0.0	47.542004	5
GAGTGGA	153080	0.0	47.452755	4
GAATCAC	157655	0.0	47.369583	9
CGAGTGG	149760	0.0	47.30349	3
>>END_MODULE