FastQCFastQC Report
Thu 26 May 2016
SRR2132784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227864
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1816488.153459995762757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315011.4139552504102584No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150210.6742332566081233No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG76980.3455327614252935No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63740.28610364007856853No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51090.22932279528732452No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44460.1995633485706488No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38970.17492091079168207No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC29320.13160587899440898No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29070.13048372791157808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT35700.088.475021
AACGAGT445250.081.532261
ACGAGTG491450.080.568572
CGAGTGG511550.079.849553
GTGGAAT544050.079.6901556
TGGAATC546700.079.291427
GGAATCA550450.078.6879358
GAATCAC550800.078.646479
GAGTGGA534950.078.377924
AGTGGAA538700.077.806145
AACGTGT17500.075.853821
TCGAGTG12650.073.215912
ATCGAGT12150.072.836311
CGTGTGG20000.072.3954853
ACGTGTG19400.070.7637562
AGCGAGT7100.053.039331
GTGTGGA28950.051.6379934
TGAGTGG25100.049.8194243
ATGAGTG24200.049.5398372
GCGAGTG7850.049.110462