##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132784_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227864 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.84548114247548 31.0 25.0 33.0 16.0 34.0 2 28.115107116053764 31.0 25.0 34.0 16.0 34.0 3 29.18678833178327 31.0 28.0 34.0 16.0 34.0 4 34.72201040997116 35.0 35.0 37.0 32.0 37.0 5 33.777096806627334 35.0 35.0 37.0 28.0 37.0 6 35.23197376500541 37.0 35.0 37.0 32.0 37.0 7 35.617641382059226 37.0 35.0 37.0 33.0 37.0 8 35.90513828492224 37.0 35.0 37.0 35.0 37.0 9 37.873639504027174 39.0 38.0 39.0 35.0 39.0 10-11 37.87262418172743 39.0 38.0 39.0 35.0 39.0 12-13 37.862835657831894 39.0 38.0 39.0 35.0 39.0 14-15 39.285074178675174 41.0 39.0 41.0 36.0 41.0 16-17 39.288631173177535 40.5 39.0 41.0 36.0 41.0 18-19 39.224811074643696 40.5 39.0 41.0 36.0 41.0 20-21 39.17955763906593 40.5 39.0 41.0 36.0 41.0 22-23 39.06250089772087 40.0 39.0 41.0 35.5 41.0 24-25 38.98607971581748 40.0 39.0 41.0 35.0 41.0 26-27 38.818098636182455 40.0 39.0 41.0 35.0 41.0 28-29 38.645331357748944 40.0 39.0 41.0 35.0 41.0 30-31 38.509189968507954 40.0 38.5 41.0 34.5 41.0 32-33 38.34407216957588 40.0 38.0 41.0 34.0 41.0 34-35 38.26376497847265 40.0 38.0 41.0 34.0 41.0 36-37 38.093788265351925 40.0 38.0 41.0 34.0 41.0 38-39 37.56040539278879 40.0 36.0 41.0 33.5 41.0 40-41 37.28635791951394 40.0 35.0 41.0 33.0 41.0 42-43 37.12345098264527 40.0 35.0 41.0 33.0 41.0 44-45 36.9727247713505 40.0 35.0 41.0 33.0 41.0 46-47 36.84138618874401 40.0 35.0 41.0 33.0 41.0 48-49 36.67330030019786 39.5 35.0 41.0 32.0 41.0 50-51 36.37995048171702 39.0 35.0 41.0 31.0 41.0 52-53 35.878852569097575 39.0 35.0 41.0 30.0 41.0 54-55 34.90230058926397 39.0 35.0 41.0 24.0 41.0 56-57 34.055875268867396 39.0 35.0 41.0 11.5 41.0 58-59 33.672671671161254 39.0 34.0 41.0 6.0 41.0 60-61 33.42360148554849 38.0 34.0 40.0 2.0 41.0 62-63 33.14326435545438 37.5 34.0 40.0 2.0 41.0 64-65 32.87126548119634 37.0 34.0 40.0 2.0 41.0 66-67 32.569826973280236 36.5 34.0 40.0 2.0 41.0 68-69 32.25118207395065 36.0 33.0 39.5 2.0 41.0 70-71 31.907286979815645 35.5 33.0 39.0 2.0 41.0 72-73 31.56638623363006 35.0 33.0 39.0 2.0 40.5 74-75 31.215249674127325 35.0 33.0 37.5 2.0 40.0 76-77 30.86390776097643 35.0 33.0 37.0 2.0 39.0 78-79 30.510083874060534 35.0 32.5 37.0 2.0 39.0 80-81 30.180127916246235 35.0 32.0 36.0 2.0 39.0 82-83 29.871787281449855 35.0 32.0 36.0 2.0 37.0 84-85 29.584642509596634 35.0 32.0 35.5 2.0 37.0 86-87 29.35498239569381 35.0 32.0 35.0 2.0 37.0 88-89 29.124342419465464 35.0 31.0 35.0 2.0 36.0 90-91 28.91753468793427 35.0 31.0 35.0 2.0 36.0 92-93 28.729900029804334 35.0 31.0 35.0 2.0 36.0 94-95 28.53469713591135 35.0 31.0 35.0 2.0 35.5 96-97 28.347048338677766 35.0 31.0 35.0 2.0 35.0 98-99 28.1727928634782 34.0 31.0 35.0 2.0 35.0 100 28.060825077293767 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36539550069777604 1101 2 2.4258750827738993 1101 3 1.297142290703622 1101 4 0.6543833879371803 1101 5 2.3166410342233377 1101 6 1.0035936019568084 1101 7 0.5432315249732511 1101 8 0.42910825968775157 1101 9 0.31066828537667135 1101 10-11 0.47874179130621997 1101 12-13 0.39677422179191524 1101 14-15 0.5282382184407126 1101 16-17 0.45693996046533414 1101 18-19 0.43335200244889904 1101 20-21 0.44790384067241007 1101 22-23 0.3796157138349727 1101 24-25 0.33697763036411743 1101 26-27 0.28013587933328665 1101 28-29 0.30764890666517886 1101 30-31 0.3555634472713294 1101 32-33 0.36941413852595417 1101 34-35 0.37777821981524085 1101 36-37 0.35141418616288433 1101 38-39 0.36774861524270364 1101 40-41 0.39091777201240063 1101 42-43 0.42633074843674024 1101 44-45 0.5947634276644678 1101 46-47 0.5330101514345884 1101 48-49 0.47772070740263217 1101 50-51 0.5219944388365292 1101 52-53 0.6234403315863517 1101 54-55 0.5714495397989552 1101 56-57 0.5389828312494274 1101 58-59 0.5650268073494544 1101 60-61 0.55207944919988 1101 62-63 0.5379923489363705 1101 64-65 0.5539411373862109 1101 66-67 0.5780095599501536 1101 68-69 0.6440785409872873 1101 70-71 0.6078270621155113 1101 72-73 0.6551710724054054 1101 74-75 0.6996276661094001 1101 76-77 0.6124882480155343 1101 78-79 0.6945881557598881 1101 80-81 0.6348635070236313 1101 82-83 0.6044773008982709 1101 84-85 0.6822216769298386 1101 86-87 0.7107069824696417 1101 88-89 0.711401865387078 1101 90-91 0.6724729168260559 1101 92-93 0.7324213071214096 1101 94-95 0.7357764028319913 1101 96-97 0.7176625943819808 1101 98-99 0.7560767410059555 1101 100 0.7648146368390734 1102 1 1.4224898433025217 1102 2 3.0062604477946344 1102 3 3.230945491382677 1102 4 1.4009870341484145 1102 5 2.708772694480551 1102 6 1.2457358761697392 1102 7 0.7376071890924152 1102 8 0.5492188748833797 1102 9 0.4299638443810778 1102 10-11 0.5499005215574257 1102 12-13 0.4944715760113638 1102 14-15 0.6442674651546128 1102 16-17 0.5537400208774912 1102 18-19 0.48577194700536097 1102 20-21 0.4531624544464776 1102 22-23 0.44089868488210726 1102 24-25 0.47345031933399184 1102 26-27 0.45254532346825727 1102 28-29 0.4897412512170405 1102 30-31 0.5268442342785917 1102 32-33 0.48344200848804064 1102 34-35 0.5114063910612998 1102 36-37 0.5757229233531191 1102 38-39 0.5792233545509546 1102 40-41 0.6626810122312321 1102 42-43 0.6314100005509786 1102 44-45 0.5998935685051592 1102 46-47 0.6985844993933696 1102 48-49 0.7324476897213188 1102 50-51 0.7913832826354081 1102 52-53 0.9183222761646164 1102 54-55 1.0033396141743225 1102 56-57 0.980292249794374 1102 58-59 0.9589489097283419 1102 60-61 0.934878633904674 1102 62-63 0.8182748011619907 1102 64-65 0.86268579698298 1102 66-67 0.9819318610139263 1102 68-69 1.028359197878352 1102 70-71 0.9828071376068053 1102 72-73 0.984632478015822 1102 74-75 1.0409362182089872 1102 76-77 1.0427890673890161 1102 78-79 0.993913135066034 1102 80-81 0.9249449708143551 1102 82-83 0.9218546693752252 1102 84-85 0.8354300568906297 1102 86-87 0.825992468000976 1102 88-89 0.8031457689355754 1102 90-91 0.8674976306862092 1102 92-93 0.8690395365044559 1102 94-95 0.8044156444114456 1102 96-97 0.7916679337768464 1102 98-99 0.8638536931708529 1102 100 0.7762809208356636 1103 1 1.559065401461524 1103 2 3.1233503124896522 1103 3 3.2265599563548975 1103 4 1.4202428279092132 1103 5 2.67659884360431 1103 6 1.2855833323115036 1103 7 0.7346801839057662 1103 8 0.49730305363263483 1103 9 0.4024222923519645 1103 10-11 0.4769911818927923 1103 12-13 0.45814540497867284 1103 14-15 0.5529823647309584 1103 16-17 0.4294409627311708 1103 18-19 0.36891320718437015 1103 20-21 0.32141565488096546 1103 22-23 0.3239772451420677 1103 24-25 0.33634700082185987 1103 26-27 0.3930076424024165 1103 28-29 0.43015845503703787 1103 30-31 0.45039054746570173 1103 32-33 0.37835391726383705 1103 34-35 0.41458978846866046 1103 36-37 0.36976161684248154 1103 38-39 0.3438178777778873 1103 40-41 0.4474260642707293 1103 42-43 0.5260548941542638 1103 44-45 0.5283554112082172 1103 46-47 0.4825242538518424 1103 48-49 0.630349015327333 1103 50-51 0.6953005573711692 1103 52-53 0.6193805210742482 1103 54-55 0.5191422999794142 1103 56-57 0.38427366582664746 1103 58-59 0.4090543320749944 1103 60-61 0.4909807197508087 1103 62-63 0.37487041464682846 1103 64-65 0.4010040957886005 1103 66-67 0.4403405543649086 1103 68-69 0.4882702389449278 1103 70-71 0.47593443372108624 1103 72-73 0.47898105324304296 1103 74-75 0.5010319717957081 1103 76-77 0.41907823537982836 1103 78-79 0.45682843915055216 1103 80-81 0.38111779946570934 1103 82-83 0.4032229920500541 1103 84-85 0.3882187763247771 1103 86-87 0.39003236359912563 1103 88-89 0.4109161915243824 1103 90-91 0.45645476239120697 1103 92-93 0.42410390265583686 1103 94-95 0.46237245151824524 1103 96-97 0.4409580265554318 1103 98-99 0.4833956291829189 1103 100 0.4807754816537191 1104 1 6.133536620330165 1104 2 5.215624506787169 1104 3 4.159892727542363 1104 4 1.847603467340008 1104 5 2.873287233061916 1104 6 1.313014898884873 1104 7 0.7374359197289095 1104 8 0.5171735739290497 1104 9 0.4479012295755709 1104 10-11 0.4979527029157609 1104 12-13 0.3972066729807011 1104 14-15 0.589838802167435 1104 16-17 0.4462928288158565 1104 18-19 0.47895447099518407 1104 20-21 0.45811483200999703 1104 22-23 0.4811195365186691 1104 24-25 0.43313569875931535 1104 26-27 0.4943910879990483 1104 28-29 0.4771018283324935 1104 30-31 0.5648466492181541 1104 32-33 0.5886049282970163 1104 34-35 0.614612831841626 1104 36-37 0.6385084444045077 1104 38-39 0.6243978298498831 1104 40-41 0.5834574051932151 1104 42-43 0.634761798761609 1104 44-45 0.6503579756992508 1104 46-47 0.5958342346319867 1104 48-49 0.6624187691516781 1104 50-51 0.7013408723534837 1104 52-53 0.5628587696230838 1104 54-55 0.5957588195481662 1104 56-57 0.45443088742896975 1104 58-59 0.47893470835516894 1104 60-61 0.4293226599060489 1104 62-63 0.5071291808048315 1104 64-65 0.47813062450283184 1104 66-67 0.5631252734154586 1104 68-69 0.5377721247139391 1104 70-71 0.4951188711089074 1104 72-73 0.5128943805789241 1104 74-75 0.5708330716847918 1104 76-77 0.5538324799497758 1104 78-79 0.5485585154107078 1104 80-81 0.5458483689397511 1104 82-83 0.4676777391231184 1104 84-85 0.4883811443264783 1104 86-87 0.4946034873084457 1104 88-89 0.48643759794699903 1104 90-91 0.5757565730722725 1104 92-93 0.5174984029892542 1104 94-95 0.5181798514959048 1104 96-97 0.5078923207342889 1104 98-99 0.5345666389332919 1104 100 0.5580193007216323 1105 1 1.5214155490503067 1105 2 3.0589930027705208 1105 3 3.2539566894817895 1105 4 1.3863506039364495 1105 5 2.6044263458226666 1105 6 1.206556558077068 1105 7 0.7446469537940317 1105 8 0.4783700390186283 1105 9 0.3222263524015858 1105 10-11 0.38022659052357 1105 12-13 0.32926950178966763 1105 14-15 0.4464932120535394 1105 16-17 0.42042944537364235 1105 18-19 0.4178897061612048 1105 20-21 0.3753387547886078 1105 22-23 0.31625826142209945 1105 24-25 0.3973479108017557 1105 26-27 0.3458754372630679 1105 28-29 0.4557782373662178 1105 30-31 0.4541317645751306 1105 32-33 0.4397628468377377 1105 34-35 0.4016463430402908 1105 36-37 0.45649549073103657 1105 38-39 0.41930716174594096 1105 40-41 0.4011246119247005 1105 42-43 0.5220363821751235 1105 44-45 0.6264583747546908 1105 46-47 0.6563428645253211 1105 48-49 0.6367782949492167 1105 50-51 0.6492090568550708 1105 52-53 0.8861562947835253 1105 54-55 0.8967058627544446 1105 56-57 0.795824525101196 1105 58-59 0.6905114543654278 1105 60-61 0.8138079729696912 1105 62-63 0.7244453046343295 1105 64-65 0.7164807713287047 1105 66-67 0.6605681398849086 1105 68-69 0.7398740219260418 1105 70-71 0.7746715536573348 1105 72-73 0.741541944932802 1105 74-75 0.7924564298823071 1105 76-77 0.7923680617140114 1105 78-79 0.8208900054025214 1105 80-81 0.7039473163161816 1105 82-83 0.661432617917022 1105 84-85 0.7131438501191703 1105 86-87 0.8112619456421868 1105 88-89 0.7307463965188568 1105 90-91 0.7603093874432751 1105 92-93 0.8575503043870647 1105 94-95 0.9250383757137506 1105 96-97 0.89670233125835 1105 98-99 0.9517432686678973 1105 100 1.03704483570381 1106 1 -0.5382831530045245 1106 2 1.8614535022736298 1106 3 2.7618157993570804 1106 4 1.2354623864946603 1106 5 2.4952476667219585 1106 6 1.104870174622775 1106 7 0.6534312971421912 1106 8 0.44458088567773757 1106 9 0.3572627577727161 1106 10-11 0.43558793390853623 1106 12-13 0.40378685038782436 1106 14-15 0.44601378125553026 1106 16-17 0.371428794560714 1106 18-19 0.3153555092041316 1106 20-21 0.3984097803961717 1106 22-23 0.36547923081548817 1106 24-25 0.32392650859267746 1106 26-27 0.3480495539810988 1106 28-29 0.4349300557944389 1106 30-31 0.440220742748636 1106 32-33 0.46573680762834613 1106 34-35 0.5604252490053483 1106 36-37 0.5563269945940874 1106 38-39 0.6409429482592301 1106 40-41 0.5733032165750842 1106 42-43 0.595763950878549 1106 44-45 0.6432520461426776 1106 46-47 0.6550419706195356 1106 48-49 0.6315773334525829 1106 50-51 0.693688314493329 1106 52-53 0.7531647009777913 1106 54-55 0.9118186508102681 1106 56-57 0.927803301740191 1106 58-59 0.9030000985999607 1106 60-61 0.9334715997209457 1106 62-63 0.9368224825153888 1106 64-65 0.9856872814656583 1106 66-67 0.9468981196076527 1106 68-69 0.8724622037320557 1106 70-71 0.8800928964734638 1106 72-73 0.99660801750262 1106 74-75 0.9283692577565112 1106 76-77 0.8830466722200434 1106 78-79 0.8561980707983459 1106 80-81 0.875528749911517 1106 82-83 0.8985049259104798 1106 84-85 0.8468221226709218 1106 86-87 0.8302961325610134 1106 88-89 0.8600607508756255 1106 90-91 0.8443926613836545 1106 92-93 0.9090244416834956 1106 94-95 0.9776530041146252 1106 96-97 1.0626064049457646 1106 98-99 1.088820323915055 1106 100 1.133347582502445 1107 1 -1.292442711955303 1107 2 1.4256853840164503 1107 3 -0.8353835228663478 1107 4 -0.13889696821031094 1107 5 1.8230488674742844 1107 6 0.74271327048708 1107 7 0.2908484054856473 1107 8 0.20963925928020188 1107 9 0.24848855588125218 1107 10-11 0.2321109058561106 1107 12-13 0.2266606687003474 1107 14-15 0.30574930766390196 1107 16-17 0.20923642316044777 1107 18-19 0.1835275853417997 1107 20-21 0.08784348323564473 1107 22-23 0.15849588806859316 1107 24-25 0.13675443816019595 1107 26-27 0.17867054489653356 1107 28-29 0.15057245068106084 1107 30-31 0.1625244224060225 1107 32-33 0.13807158057129243 1107 34-35 0.11593912497832548 1107 36-37 0.21457945685900626 1107 38-39 0.2591207295868969 1107 40-41 0.1496671848770461 1107 42-43 0.21587293902506843 1107 44-45 0.17169669724178505 1107 46-47 0.19747955108648796 1107 48-49 0.17896939601188677 1107 50-51 0.25279313214595334 1107 52-53 0.30644832308882997 1107 54-55 0.2344067341897187 1107 56-57 0.27227649400319365 1107 58-59 0.2515957660786654 1107 60-61 0.34434892482359913 1107 62-63 0.3505680918962426 1107 64-65 0.3415703783188704 1107 66-67 0.3636261170669428 1107 68-69 0.3587768791923196 1107 70-71 0.4626593571827726 1107 72-73 0.422300770199751 1107 74-75 0.43142723960259133 1107 76-77 0.428924006831334 1107 78-79 0.49599709597154984 1107 80-81 0.405984955922122 1107 82-83 0.4339631572635412 1107 84-85 0.36383785187978646 1107 86-87 0.5017442238896948 1107 88-89 0.4407145466336253 1107 90-91 0.4008735670635488 1107 92-93 0.4018178358341551 1107 94-95 0.39101398860221437 1107 96-97 0.41439555253332827 1107 98-99 0.39851277613884406 1107 100 0.4128600549986743 1108 1 -1.325498166663337 1108 2 0.3000288794537518 1108 3 1.98997762851981 1108 4 0.8803714493398687 1108 5 0.7803150698756411 1108 6 0.5025318063550657 1108 7 0.31660346344717283 1108 8 0.2547868476002435 1108 9 0.19020258676899005 1108 10-11 0.2848429227391449 1108 12-13 0.2445457311827397 1108 14-15 0.28769649716504375 1108 16-17 0.22691459258479796 1108 18-19 0.18075473480754312 1108 20-21 0.18775755603160604 1108 22-23 0.11363682173156775 1108 24-25 0.10649132975223097 1108 26-27 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64-65 -0.4118091209211272 2215 66-67 -0.4530665898234929 2215 68-69 -0.4406614396895492 2215 70-71 -0.44359491934404005 2215 72-73 -0.4170153059362782 2215 74-75 -0.44851942939606815 2215 76-77 -0.44606514539332665 2215 78-79 -0.4965267007097687 2215 80-81 -0.3910278838385466 2215 82-83 -0.3268731861883758 2215 84-85 -0.30325694032023165 2215 86-87 -0.25305541603091797 2215 88-89 -0.31144324277000024 2215 90-91 -0.3156883323452 2215 92-93 -0.34465480140355353 2215 94-95 -0.3868473541320796 2215 96-97 -0.4151428395793708 2215 98-99 -0.4231813392935031 2215 100 -0.38588350559499673 2216 1 2.8595147241747974 2216 2 -1.8792910090894388 2216 3 -2.264454126397382 2216 4 -0.8890006967313084 2216 5 -2.5461775808888554 2216 6 -1.0660316947327075 2216 7 -0.5847924539681628 2216 8 -0.40926284703197524 2216 9 -0.45504300556397936 2216 10-11 -0.1802817025796557 2216 12-13 -0.27276093054139494 2216 14-15 -0.3204545109859609 2216 16-17 -0.42365435361521975 2216 18-19 -0.4323933300395524 2216 20-21 -0.41189705697252066 2216 22-23 -0.3782111288542538 2216 24-25 -0.413236407693951 2216 26-27 -0.41232263422614324 2216 28-29 -0.4110411136303469 2216 30-31 -0.4036821411027063 2216 32-33 -0.31792363711457483 2216 34-35 -0.34730434815723754 2216 36-37 -0.3115688552605249 2216 38-39 -0.2613715391068112 2216 40-41 -0.2377987259701584 2216 42-43 -0.2814316428118886 2216 44-45 -0.4083323495138771 2216 46-47 -0.38743865433531965 2216 48-49 -0.38641855696598526 2216 50-51 -0.4476813462062239 2216 52-53 -0.5202259577221469 2216 54-55 -0.27589791190629853 2216 56-57 0.013062728494460885 2216 58-59 0.054131007462828507 2216 60-61 0.015947612609330974 2216 62-63 0.12603399775783686 2216 64-65 0.1280790784966328 2216 66-67 0.17740420062919782 2216 68-69 0.13564360480005888 2216 70-71 0.26149104720151684 2216 72-73 0.3021678398150236 2216 74-75 0.2044301124256087 2216 76-77 0.15485317340798233 2216 78-79 0.1504038546948543 2216 80-81 0.0964402839673788 2216 82-83 0.09825036921262154 2216 84-85 0.14858482907280646 2216 86-87 0.1360091612121863 2216 88-89 0.10046263174142567 2216 90-91 0.2133526205497951 2216 92-93 0.1275358754011151 2216 94-95 -0.027230348706869023 2216 96-97 -0.04639305843513952 2216 98-99 -0.0705915661669394 2216 100 -0.1008683939815711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1073.0 3 241.0 4 272.0 5 614.0 6 1367.0 7 2381.0 8 2972.0 9 4009.0 10 3524.0 11 4551.0 12 4345.0 13 6206.0 14 6407.0 15 7175.0 16 8603.0 17 10421.0 18 14846.0 19 27002.0 20 54461.0 21 65031.0 22 28625.0 23 17759.0 24 16767.0 25 17214.0 26 17997.0 27 19599.0 28 23096.0 29 26561.0 30 30830.0 31 37245.0 32 47773.0 33 65250.0 34 95495.0 35 171974.0 36 266680.0 37 447909.0 38 524978.0 39 146075.0 40 536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.375320114373753 42.863011272879916 24.459884241968265 19.301784370778062 2 14.661819344013423 42.7882010576548 24.447482874045175 18.102496724286603 3 17.819339588552438 24.6445697602964 18.72063517570977 38.81545547544139 4 35.099763719868 25.978650402358493 20.813478740174446 18.108107137599063 5 17.030498492917946 41.72761203241742 21.29279136732628 19.949098107338354 6 38.77192257897695 22.92459369046315 22.639849309484774 15.663634421075123 7 18.38081588077212 22.129310472524253 41.872321630163555 17.617552016540078 8 37.83238115073452 22.64842019081955 22.362855183260738 17.156343475185203 9 37.156846198870305 24.087646283615157 22.520674511550077 16.234833005964457 10-11 16.258465507768875 44.48871205782759 22.518452652406072 16.734369781997465 12-13 16.171521241871137 23.857762412786418 32.55180747119214 27.418908874150304 14-15 16.487720973991234 33.792008847936856 22.464275198126995 27.255994979944916 16-17 26.99289543706438 23.40189975689719 32.704868878890274 16.900335927148156 18-19 16.685443994786038 23.632142716072437 32.82103395898493 26.8613793301566 20-21 27.053738468775475 34.10850033933849 22.454243167446485 16.383518024439553 22-23 26.641998793463156 23.907024845322695 32.82433308316845 16.626643278045698 24-25 16.46675919176395 34.046714700717814 22.44605146454182 27.040474642976413 26-27 16.910917976311374 27.866217057830095 38.65819816079314 16.564666805065393 28-29 25.651633133799912 23.640671064301948 34.15726902539832 16.550426776499823 30-31 23.277991834331 24.896627442249617 34.55738321549251 17.267997507926875 32-33 19.074436321068074 26.66854888808293 35.797090845760785 18.45992394508821 34-35 18.770894453162313 26.37326605214681 36.196509302183614 18.659330192507262 36-37 18.87072101349095 26.50002423846339 36.21073817791392 18.41851657013175 38-39 19.35901383567399 26.271599164042332 35.85032569313028 18.519061307153397 40-41 18.678653634153612 27.180272224875484 35.675808756728415 18.465265384242485 42-43 18.68665400270396 26.698368467451044 36.00406940521868 18.61090812462632 44-45 18.714831785064078 27.04074395923629 35.494042724331464 18.750381531368163 46-47 19.156308464071415 26.631360801197918 35.63045140996039 18.581879324770274 48-49 18.682334289705295 27.021398074568285 35.478736583561655 18.817531052164764 50-51 19.04346495118194 27.292442447115263 34.8335445969772 18.830548004725603 52-53 19.226770054256043 27.242053762294578 34.495216827788965 19.035959355660413 54-55 19.691956061949924 28.11257778751306 32.54271804742121 19.65274810311581 56-57 20.156010420743815 28.986711038016683 30.833928821507957 20.023349719731545 58-59 20.673726044318684 29.65659034842342 29.57781534240869 20.0918682648492 60-61 20.508926936294138 29.93993349683823 29.34431814509324 20.2068214217744 62-63 19.940943419558693 30.245434695617572 29.073639385895948 20.739982498927784 64-65 20.13648499190256 30.032510961171777 29.74674396641806 20.084260080507608 66-67 20.132804336350873 29.674365221575467 30.013860810175125 20.178969631898536 68-69 20.225538901836018 29.74387125964601 29.85523353310615 20.17535630541182 70-71 19.364445046914895 30.331362237551307 30.05185684583978 20.252335869694022 72-73 19.402371741626986 29.88403876102433 30.167674623567542 20.545914873781143 74-75 19.667451949933085 30.14192777503598 29.607984528640397 20.582635746390537 76-77 20.12498131609222 29.758987689044115 29.600898263933935 20.515132730929732 78-79 20.093744774968776 30.060899316944646 29.13584015135618 20.7095157567304 80-81 20.17701867814802 30.45004497621916 28.88774977063026 20.485186575002558 82-83 20.17490035191037 30.67565444556162 28.645908144211436 20.503537058316574 84-85 20.105106550778345 30.81030031629369 28.258405191229418 20.826187941698542 86-87 20.109441150806333 30.99621431110696 28.171490719361685 20.72285381872502 88-89 20.784751672453975 30.601306902037106 28.045675139954685 20.568266285554234 90-91 20.370691638285578 31.07162134108366 27.94640602819968 20.611280992431087 92-93 20.044154400807233 31.12483526822104 27.88814757094688 20.94286276002485 94-95 20.083609232879564 31.389326278444287 27.61660945192346 20.910455036752694 96-97 20.020953091871544 30.84067997002469 27.7914395308267 21.346927407277068 98-99 20.533671357211805 30.994561798468972 27.092818840402444 21.378948003916783 100 20.284451833684642 31.41637011954051 26.883867237856528 21.415310808918317 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 33153.0 1 18297.0 2 2602.0 3 1545.5 4 1281.0 5 1183.0 6 1149.0 7 1269.0 8 1239.0 9 1149.0 10 1202.5 11 1949.0 12 14158.0 13 16390.0 14 6687.0 15 6653.5 16 7571.0 17 8756.5 18 10013.5 19 11479.5 20 12938.5 21 14462.5 22 16377.0 23 18727.0 24 21137.0 25 23749.0 26 27388.5 27 31061.0 28 34225.5 29 37036.5 30 39682.0 31 42791.5 32 46421.5 33 50197.0 34 54136.0 35 57698.5 36 60556.5 37 63200.5 38 65381.5 39 66592.0 40 67141.5 41 67975.5 42 69199.5 43 69824.5 44 70062.5 45 70381.5 46 70799.5 47 71743.5 48 71227.5 49 70154.0 50 74464.0 51 72540.5 52 62232.0 53 56540.0 54 53455.0 55 51374.0 56 46179.5 57 39475.0 58 35032.5 59 28810.5 60 22287.5 61 17557.5 62 13839.5 63 10469.0 64 7819.5 65 5850.0 66 4341.0 67 3285.0 68 2426.5 69 1862.5 70 1370.5 71 975.5 72 697.0 73 510.0 74 360.0 75 246.5 76 175.0 77 105.5 78 68.0 79 47.5 80 22.0 81 11.0 82 8.0 83 5.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12918203265549424 2 0.004713034547889817 3 0.0031869090752397813 4 0.0 5 0.0021994161223485814 6 0.011311282914935562 7 0.0028278207287338905 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.732906496985453E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.977208662647271E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.732906496985453E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.2443021656618178E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.366453248492727E-4 74-75 2.4687323822279993E-4 76-77 4.937464764455999E-4 78-79 2.917592815360363E-4 80-81 8.977208662647271E-4 82-83 0.0010772650395176725 84-85 0.0013241382777404726 86-87 0.0 88-89 0.0 90-91 6.732906496985453E-5 92-93 0.0 94-95 0.0 96-97 0.001369024321053709 98-99 6.059615847286907E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2227864.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.48850227059287 #Duplication Level Percentage of deduplicated Percentage of total 1 92.5843800540734 66.1871866371706 2 5.219997082173708 7.463395465229267 3 0.8674340865302412 1.8603469099352041 4 0.3468628370122041 0.9918681884532498 5 0.1725130517187512 0.6166349844751425 6 0.10463925098062989 0.44883019987931394 7 0.08301094800761243 0.41540298415883875 8 0.056001809069176345 0.3202788363839298 9 0.04934163451003252 0.3174623595634701 >10 0.48445313439005677 6.085169125266412 >50 0.017007794521420806 0.8365697396546367 >100 0.012352910872439083 1.8875061376903437 >500 0.0010653720120538948 0.46324324932842875 >1k 5.640204769696526E-4 1.0245112790755917 >5k 1.8800682565655086E-4 0.8593296678305461 >10k+ 1.8800682565655086E-4 10.222264235905099 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 181648 8.153459995762757 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31501 1.4139552504102584 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 15021 0.6742332566081233 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 7698 0.3455327614252935 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6374 0.28610364007856853 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5109 0.22932279528732452 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4446 0.1995633485706488 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3897 0.17492091079168207 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 2932 0.13160587899440898 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2907 0.13048372791157808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0011670371261441452 0.0 0.0 0.0 0.0 2 0.0011670371261441452 4.4886043313236356E-5 0.0 4.4886043313236356E-5 0.0 3 0.0011670371261441452 4.4886043313236356E-5 0.0 4.4886043313236356E-5 0.0 4 0.0011670371261441452 8.977208662647271E-5 0.0 4.4886043313236356E-5 0.0 5 0.0011670371261441452 1.3465812993970907E-4 0.0 4.4886043313236356E-5 0.0 6 0.0011670371261441452 1.3465812993970907E-4 0.0 4.4886043313236356E-5 0.0 7 0.0011670371261441452 1.3465812993970907E-4 0.0 4.4886043313236356E-5 0.0 8 0.0011670371261441452 1.3465812993970907E-4 0.0 4.4886043313236356E-5 0.0 9 0.0011670371261441452 1.3465812993970907E-4 0.0 4.4886043313236356E-5 0.0 10-11 0.0011670371261441452 2.468732382228E-4 0.0 4.4886043313236356E-5 0.0 12-13 0.0011670371261441452 3.142023031926545E-4 0.0 8.977208662647271E-5 0.0 14-15 0.0011670371261441452 3.366453248492727E-4 0.0 1.1221510828309089E-4 0.0 16-17 0.0011670371261441452 3.5908834650589085E-4 0.0 1.5710115159632725E-4 0.0 18-19 0.0011894801478007635 3.5908834650589085E-4 0.0 1.7954417325294542E-4 0.0 20-21 0.0012119231694573817 4.039743898191272E-4 0.0 1.7954417325294542E-4 0.0 22-23 0.0012119231694573817 4.039743898191272E-4 0.0 1.7954417325294542E-4 0.0 24-25 0.0012119231694573817 4.039743898191272E-4 0.0 2.2443021656618178E-4 0.0 26-27 0.001256809212770618 4.264174114757454E-4 0.0 3.142023031926545E-4 0.0 28-29 0.0012792522344272361 4.4886043313236356E-4 0.0 3.142023031926545E-4 0.0 30-31 0.0013465812993970906 4.4886043313236356E-4 0.0 3.142023031926545E-4 0.0 32-33 0.0013690243210537087 5.386325197588363E-4 0.0 3.366453248492727E-4 0.0 34-35 0.001638340580933127 5.835185630720726E-4 0.0 3.5908834650589085E-4 0.0 36-37 0.0022891882089750544 5.835185630720726E-4 0.0 3.5908834650589085E-4 0.0 38-39 0.0033664532484927265 6.284046063853089E-4 0.0 3.5908834650589085E-4 0.0 40-41 0.0051170089377089445 6.732906496985453E-4 0.0 4.039743898191272E-4 0.0 42-43 0.007675513406563417 6.732906496985453E-4 0.0 4.264174114757454E-4 0.0 44-45 0.010458448091984072 6.732906496985453E-4 0.0 4.4886043313236356E-4 0.0 46-47 0.013959559470416506 7.181766930117817E-4 0.0 4.937464764455999E-4 0.0 48-49 0.017842202217011452 7.181766930117817E-4 0.0 4.937464764455999E-4 0.0 50-51 0.02266745187318436 7.181766930117817E-4 0.0 4.937464764455999E-4 0.0 52-53 0.028569966568874942 7.63062736325018E-4 0.0 5.386325197588363E-4 0.0 54-55 0.03638013810537807 7.63062736325018E-4 0.0 5.610755414154544E-4 0.0 56-57 0.04524513165974225 7.855057579816362E-4 0.0 6.059615847286907E-4 0.0 58-59 0.057050161051123406 8.079487796382544E-4 0.0 6.28404606385309E-4 0.0 60-61 0.06982024037373916 8.079487796382544E-4 0.0 6.28404606385309E-4 0.0 62-63 0.08579967179325129 8.303918012948726E-4 0.0 6.28404606385309E-4 0.0 64-65 0.10622282150077383 8.977208662647271E-4 0.0 6.28404606385309E-4 0.0 66-67 0.1322118405791377 8.977208662647271E-4 0.0 6.732906496985453E-4 0.0 68-69 0.16313832442195753 9.426069095779634E-4 0.0 6.732906496985453E-4 0.0 70-71 0.2051741039848034 9.426069095779634E-4 0.0 6.732906496985453E-4 0.0 72-73 0.2539876760879479 9.426069095779634E-4 0.0 6.732906496985453E-4 0.0 74-75 0.31085829296581835 9.426069095779634E-4 0.0 8.079487796382544E-4 0.0 76-77 0.3761674859865773 0.0010323789962044362 0.0 8.528348229514907E-4 0.0 78-79 0.4558626558892284 0.0010323789962044362 0.0 8.528348229514907E-4 0.0 80-81 0.551514814189735 0.0010772650395176725 0.0 8.75277844608109E-4 0.0 82-83 0.6648296305340002 0.0011221510828309088 0.0 8.977208662647271E-4 0.0 84-85 0.8009689999030462 0.0011221510828309088 0.0 9.201638879213453E-4 0.0 86-87 0.958855657257355 0.0011221510828309088 0.0 9.426069095779634E-4 0.0 88 1.0962518358391715 0.0011221510828309088 0.0 9.874929528911997E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3570 0.0 88.47502 1 AACGAGT 44525 0.0 81.53226 1 ACGAGTG 49145 0.0 80.56857 2 CGAGTGG 51155 0.0 79.84955 3 GTGGAAT 54405 0.0 79.690155 6 TGGAATC 54670 0.0 79.29142 7 GGAATCA 55045 0.0 78.687935 8 GAATCAC 55080 0.0 78.64647 9 GAGTGGA 53495 0.0 78.37792 4 AGTGGAA 53870 0.0 77.80614 5 AACGTGT 1750 0.0 75.85382 1 TCGAGTG 1265 0.0 73.21591 2 ATCGAGT 1215 0.0 72.83631 1 CGTGTGG 2000 0.0 72.395485 3 ACGTGTG 1940 0.0 70.763756 2 AGCGAGT 710 0.0 53.03933 1 GTGTGGA 2895 0.0 51.637993 4 TGAGTGG 2510 0.0 49.819424 3 ATGAGTG 2420 0.0 49.539837 2 GCGAGTG 785 0.0 49.11046 2 >>END_MODULE