FastQCFastQC Report
Thu 26 May 2016
SRR2132783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625193
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2134778.131859257586013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA526822.0067857867973897No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT168250.6409052591561839No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG116000.4418722737718712No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71610.27277994417934226No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59380.22619289324632513No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51150.19484281727095873No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46240.17613943051044248No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42080.1602929765544857No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC40620.1547314806949432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT36500.087.446411
AACGAGT515150.078.0238951
ACGAGTG563250.077.2960742
GTGGAAT626250.076.545276
CGAGTGG587100.076.52333
TGGAATC627950.076.245547
GAATCAC636150.075.409059
GGAATCA636050.075.36188
AACGTGT21600.075.19181
GAGTGGA614300.075.120364
AGTGGAA616850.074.771715
ATCGAGT13700.074.5666051
CGTGTGG24550.072.7639543
ACGTGTG23700.071.210972
TCGAGTG15450.070.592772
GTGTGGA35900.051.1966634
TGAGTGG27050.051.0933343
AGCGAGT8750.050.035711
ATGAGTG25850.050.0118452
GCGAGTG8800.049.6822242
Produced by FastQC (version 0.11.5)