##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132783_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2625193 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.84056105589189 31.0 25.0 33.0 16.0 34.0 2 28.09337065884299 31.0 25.0 33.0 16.0 34.0 3 29.143502972924278 31.0 28.0 34.0 16.0 34.0 4 34.67414167263131 35.0 35.0 37.0 32.0 37.0 5 33.73800783409067 35.0 35.0 37.0 28.0 37.0 6 35.17544538630112 37.0 35.0 37.0 32.0 37.0 7 35.56723600893344 37.0 35.0 37.0 33.0 37.0 8 35.85089705785441 37.0 35.0 37.0 35.0 37.0 9 37.80921059899215 39.0 38.0 39.0 35.0 39.0 10-11 37.810179099212895 39.0 38.0 39.0 35.0 39.0 12-13 37.791801593254284 39.0 38.0 39.0 35.0 39.0 14-15 39.19379679894011 41.0 39.0 41.0 36.0 41.0 16-17 39.18641524642188 40.5 39.0 41.0 36.0 41.0 18-19 39.07984841495463 40.0 39.0 41.0 35.5 41.0 20-21 39.017015891783956 40.0 39.0 41.0 35.0 41.0 22-23 38.88918205251957 40.0 39.0 41.0 35.0 41.0 24-25 38.78053746905466 40.0 39.0 41.0 35.0 41.0 26-27 38.57732840975883 40.0 38.5 41.0 34.5 41.0 28-29 38.363097303703 40.0 38.0 41.0 34.0 41.0 30-31 38.215195606570646 40.0 38.0 41.0 34.0 41.0 32-33 38.042288319373085 40.0 38.0 41.0 34.0 41.0 34-35 37.94171361877012 40.0 38.0 41.0 33.5 41.0 36-37 37.75399523006499 40.0 38.0 41.0 33.0 41.0 38-39 37.21011407542227 40.0 36.0 41.0 33.0 41.0 40-41 36.933682399732135 40.0 35.0 41.0 33.0 41.0 42-43 36.75968014542169 40.0 35.0 41.0 32.0 41.0 44-45 36.59652090341548 39.0 35.0 41.0 32.0 41.0 46-47 36.4478272645097 39.0 35.0 41.0 31.0 41.0 48-49 36.269521707546836 39.0 35.0 41.0 31.0 41.0 50-51 35.95944507699053 39.0 35.0 41.0 30.5 41.0 52-53 35.464941625244315 39.0 35.0 41.0 28.0 41.0 54-55 34.52332495172736 39.0 35.0 41.0 22.0 41.0 56-57 33.722176426647486 38.0 34.0 40.5 9.5 41.0 58-59 33.3325035530721 38.0 34.0 40.0 3.5 41.0 60-61 33.06249064354506 37.5 34.0 40.0 2.0 41.0 62-63 32.77094522193225 37.0 34.0 40.0 2.0 41.0 64-65 32.48382099906559 36.5 33.0 40.0 2.0 41.0 66-67 32.16589770733047 36.0 33.0 39.5 2.0 41.0 68-69 31.832009113234722 35.0 33.0 39.0 2.0 41.0 70-71 31.494714102925006 35.0 33.0 39.0 2.0 40.5 72-73 31.15844435056775 35.0 33.0 38.0 2.0 40.0 74-75 30.811426626537553 35.0 32.5 37.0 2.0 40.0 76-77 30.47182949977392 35.0 32.0 37.0 2.0 39.0 78-79 30.122524705802583 35.0 32.0 36.0 2.0 39.0 80-81 29.79926180665574 35.0 32.0 36.0 2.0 38.5 82-83 29.497343433416134 35.0 31.0 35.5 2.0 37.0 84-85 29.21327365264192 35.0 31.0 35.0 2.0 37.0 86-87 28.988743875212222 35.0 31.0 35.0 2.0 36.5 88-89 28.752545622359953 35.0 31.0 35.0 2.0 36.0 90-91 28.53724849944366 35.0 31.0 35.0 2.0 36.0 92-93 28.34064105001042 35.0 30.5 35.0 2.0 36.0 94-95 28.13654767478048 34.5 30.0 35.0 2.0 35.0 96-97 27.935086296512296 34.0 30.0 35.0 2.0 35.0 98-99 27.74813508949628 34.0 30.0 35.0 2.0 35.0 100 27.631017986106166 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2821943738045576 1101 2 2.3566788957234373 1101 3 1.1719718685728715 1101 4 0.6917663698628473 1101 5 2.3122269958924875 1101 6 1.0308658520967526 1101 7 0.5779821271901184 1101 8 0.4173286451424474 1101 9 0.36239434818839555 1101 10-11 0.5205068294955595 1101 12-13 0.4550559978835196 1101 14-15 0.6043695573549428 1101 16-17 0.5441517372000035 1101 18-19 0.5045692442663281 1101 20-21 0.4662183987492483 1101 22-23 0.4999284776538033 1101 24-25 0.4068602365899281 1101 26-27 0.458294153777679 1101 28-29 0.4848104529942461 1101 30-31 0.5742725944240732 1101 32-33 0.5776321915726754 1101 34-35 0.5527479613682829 1101 36-37 0.5787648046157017 1101 38-39 0.627674142967038 1101 40-41 0.5679666818525959 1101 42-43 0.6223466720746202 1101 44-45 0.6974750192626615 1101 46-47 0.6686032239682262 1101 48-49 0.6620976966249685 1101 50-51 0.6993956549371134 1101 52-53 0.683688043448889 1101 54-55 0.6449389130505807 1101 56-57 0.509185932969423 1101 58-59 0.41313425377938273 1101 60-61 0.5300361972379264 1101 62-63 0.5130204890059744 1101 64-65 0.4569607336510728 1101 66-67 0.4657242427155879 1101 68-69 0.5361334768706385 1101 70-71 0.5098233187777232 1101 72-73 0.5518448807671525 1101 74-75 0.5356563593470547 1101 76-77 0.4337015635933561 1101 78-79 0.43537075739881814 1101 80-81 0.444965353624454 1101 82-83 0.375734462064127 1101 84-85 0.49762012461520655 1101 86-87 0.46194285923829526 1101 88-89 0.4114822618601792 1101 90-91 0.3468651522757966 1101 92-93 0.4405443015326789 1101 94-95 0.4536333325676587 1101 96-97 0.4177146244665195 1101 98-99 0.4864648964943541 1101 100 0.5027845658259729 1102 1 1.4447213381262998 1102 2 3.0398374037639826 1102 3 3.210354038507191 1102 4 1.4588040763925108 1102 5 2.6519129558673242 1102 6 1.2496363824856047 1102 7 0.713396326836417 1102 8 0.5163128681325233 1102 9 0.4475573531502164 1102 10-11 0.4805012055325477 1102 12-13 0.4357798955991399 1102 14-15 0.5838829704111319 1102 16-17 0.5242474232908734 1102 18-19 0.4775365206960842 1102 20-21 0.47305917210994863 1102 22-23 0.46591966711579147 1102 24-25 0.5349822332913519 1102 26-27 0.5694563764902085 1102 28-29 0.586918275194698 1102 30-31 0.583162892396274 1102 32-33 0.5714072595921493 1102 34-35 0.5709239271976188 1102 36-37 0.578308736189804 1102 38-39 0.5911906650030545 1102 40-41 0.7145461517898681 1102 42-43 0.7441204841769036 1102 44-45 0.7082660896554316 1102 46-47 0.6964132768386477 1102 48-49 0.6660104612104334 1102 50-51 0.8319543523098716 1102 52-53 0.851939197873854 1102 54-55 0.8830913675432583 1102 56-57 0.8039114799760156 1102 58-59 0.8406010717228938 1102 60-61 0.854102642994512 1102 62-63 0.7882742027220857 1102 64-65 0.8113911536118792 1102 66-67 0.8551171909564559 1102 68-69 0.8402647674492627 1102 70-71 0.8842899363232739 1102 72-73 0.888593582397526 1102 74-75 0.8678218853647159 1102 76-77 0.8853956179814979 1102 78-79 0.873129843737054 1102 80-81 0.9085932640899728 1102 82-83 0.8633139774339327 1102 84-85 0.8391927718003132 1102 86-87 0.8179329983540669 1102 88-89 0.8501139283817771 1102 90-91 0.8617429016469949 1102 92-93 0.8833396799193416 1102 94-95 0.8510384319873125 1102 96-97 0.8313987127108966 1102 98-99 0.8365555816327195 1102 100 0.756803320916049 1103 1 1.5051342855347691 1103 2 3.0210088910982513 1103 3 3.26850913374777 1103 4 1.4572790479851179 1103 5 2.6454155204853933 1103 6 1.2314010424600497 1103 7 0.7388818610162673 1103 8 0.473767099253962 1103 9 0.3625149106616661 1103 10-11 0.44984258879333083 1103 12-13 0.4672024580455627 1103 14-15 0.6251365687868073 1103 16-17 0.4652975254896248 1103 18-19 0.4350367189858275 1103 20-21 0.44933497045415294 1103 22-23 0.3773674431107281 1103 24-25 0.33588957323546964 1103 26-27 0.38196790804627057 1103 28-29 0.3911700314904536 1103 30-31 0.3749640143557116 1103 32-33 0.4292935290770288 1103 34-35 0.5323766657380631 1103 36-37 0.5177851918804208 1103 38-39 0.4520651185398421 1103 40-41 0.5282522995354455 1103 42-43 0.5387226343588054 1103 44-45 0.6280858813896941 1103 46-47 0.5960251167087591 1103 48-49 0.597594348800726 1103 50-51 0.6709648164944966 1103 52-53 0.7474751230980488 1103 54-55 0.8502156699769046 1103 56-57 0.8142064820988821 1103 58-59 0.9054377361118782 1103 60-61 0.9113610868505191 1103 62-63 0.8682192732814187 1103 64-65 0.8574533843566741 1103 66-67 0.8422820653807293 1103 68-69 0.8138039022103598 1103 70-71 0.9162558868988633 1103 72-73 0.8501452007175487 1103 74-75 0.8700095408419024 1103 76-77 0.8641050783322441 1103 78-79 0.8801498839379143 1103 80-81 0.8123595922431726 1103 82-83 0.8404547220108647 1103 84-85 0.8979936341804446 1103 86-87 0.8467450816009787 1103 88-89 0.8885447078704303 1103 90-91 0.89528419532747 1103 92-93 0.8816352364051987 1103 94-95 0.9159044687076054 1103 96-97 0.9026944799988996 1103 98-99 0.8905457439164373 1103 100 0.8818911728167258 1104 1 6.1193351084215095 1104 2 5.160266996859729 1104 3 4.171318432501209 1104 4 1.916866986813126 1104 5 2.917304301763842 1104 6 1.3234603610497402 1104 7 0.7564019713482608 1104 8 0.5739535297994962 1104 9 0.45562115971906536 1104 10-11 0.5404228190392928 1104 12-13 0.4420211606997384 1104 14-15 0.5599004143438222 1104 16-17 0.4510220819296862 1104 18-19 0.4219573563868977 1104 20-21 0.37735751655755223 1104 22-23 0.3688853984073788 1104 24-25 0.3448364829281019 1104 26-27 0.4287301461092383 1104 28-29 0.4138775505529466 1104 30-31 0.5325670251060544 1104 32-33 0.5575064729776713 1104 34-35 0.5649608244717399 1104 36-37 0.5194150963574842 1104 38-39 0.6280761771782437 1104 40-41 0.5879568634496906 1104 42-43 0.6675132525050742 1104 44-45 0.6424555093220548 1104 46-47 0.5862559865302117 1104 48-49 0.690634577944337 1104 50-51 0.7189011793544822 1104 52-53 0.6112177435556205 1104 54-55 0.691736835416144 1104 56-57 0.6244964427716084 1104 58-59 0.6476314869939088 1104 60-61 0.6208525699788012 1104 62-63 0.5907380299023473 1104 64-65 0.5558482222050998 1104 66-67 0.5604398735680505 1104 68-69 0.4689290462882312 1104 70-71 0.5491253546269341 1104 72-73 0.5356469248064002 1104 74-75 0.5854994479718698 1104 76-77 0.5772800486687082 1104 78-79 0.6148299326594007 1104 80-81 0.6214189477797909 1104 82-83 0.6155316897222853 1104 84-85 0.6352522450851161 1104 86-87 0.5665648956913216 1104 88-89 0.5934129799718093 1104 90-91 0.623649045598544 1104 92-93 0.5322493730252305 1104 94-95 0.6029744685925245 1104 96-97 0.6339798029241024 1104 98-99 0.7258236362700607 1104 100 0.671227987640556 1105 1 1.5670037365789113 1105 2 3.0795510527422003 1105 3 3.2283870046160175 1105 4 1.3750985427956763 1105 5 2.5794848589873283 1105 6 1.1687079492167456 1105 7 0.6825635541511943 1105 8 0.47377439365602925 1105 9 0.3100777467251916 1105 10-11 0.42334989361597763 1105 12-13 0.37478681259518254 1105 14-15 0.4320412413723602 1105 16-17 0.3262294895594806 1105 18-19 0.27872555021420453 1105 20-21 0.2503387322815058 1105 22-23 0.2343220302475686 1105 24-25 0.2370539682169195 1105 26-27 0.2307860586027246 1105 28-29 0.3409619847229166 1105 30-31 0.3376340260267483 1105 32-33 0.3544072175435389 1105 34-35 0.4131315842491716 1105 36-37 0.3329661451545718 1105 38-39 0.304206286060996 1105 40-41 0.38017625018360945 1105 42-43 0.30459983831028126 1105 44-45 0.4658815130849163 1105 46-47 0.4089618429811068 1105 48-49 0.3849054237871741 1105 50-51 0.4176423726613976 1105 52-53 0.45958815506836714 1105 54-55 0.43188509809578335 1105 56-57 0.4730736614578106 1105 58-59 0.49290449447959617 1105 60-61 0.4096759757671933 1105 62-63 0.371101656258368 1105 64-65 0.32135635838860566 1105 66-67 0.2772822737922169 1105 68-69 0.36973190646195775 1105 70-71 0.4614196983281502 1105 72-73 0.4194063819938627 1105 74-75 0.39193213681539163 1105 76-77 0.3538641025496574 1105 78-79 0.3304577598080307 1105 80-81 0.27256086563736304 1105 82-83 0.22344485372692802 1105 84-85 0.2721424816649183 1105 86-87 0.32699131977362583 1105 88-89 0.3317662284406424 1105 90-91 0.30415523029891744 1105 92-93 0.3132556878244799 1105 94-95 0.32965009206636253 1105 96-97 0.34003150221555956 1105 98-99 0.27246698521526014 1105 100 0.32572750727548083 1106 1 -0.7797898752146999 1106 2 1.8262463844375318 1106 3 2.7292019104309198 1106 4 1.163749238946373 1106 5 2.4065262185286898 1106 6 1.1220822325910333 1106 7 0.6440357081233472 1106 8 0.38288577776741306 1106 9 0.26527430692174647 1106 10-11 0.4120978461139231 1106 12-13 0.3335961901545659 1106 14-15 0.36549742482853986 1106 16-17 0.31168710626709384 1106 18-19 0.22151731925597318 1106 20-21 0.2462795594723346 1106 22-23 0.22583718176272072 1106 24-25 0.2307189968819543 1106 26-27 0.2448554689221396 1106 28-29 0.3768065793175097 1106 30-31 0.3485850907278234 1106 32-33 0.33957101770734255 1106 34-35 0.3711751960427776 1106 36-37 0.43380050223892397 1106 38-39 0.44194686755157875 1106 40-41 0.3632884532958087 1106 42-43 0.3586968899073355 1106 44-45 0.3691329463363431 1106 46-47 0.39396184298110626 1106 48-49 0.46036201674377253 1106 50-51 0.5424959762650019 1106 52-53 0.6411166153468244 1106 54-55 0.6120396845003668 1106 56-57 0.593931564815712 1106 58-59 0.48499908907419353 1106 60-61 0.46986434595556403 1106 62-63 0.514753376160094 1106 64-65 0.5225879308701735 1106 66-67 0.45602715503709845 1106 68-69 0.4706584011384507 1106 70-71 0.4591981586066076 1106 72-73 0.42571678330426366 1106 74-75 0.45314630552956103 1106 76-77 0.5352246676602235 1106 78-79 0.4880959661962372 1106 80-81 0.472856729683226 1106 82-83 0.4858803574124302 1106 84-85 0.4880474775699106 1106 86-87 0.5786293214116256 1106 88-89 0.651184737859154 1106 90-91 0.6023227159664017 1106 92-93 0.6178676861864787 1106 94-95 0.7150954237616993 1106 96-97 0.7937866209706819 1106 98-99 0.7645759123241866 1106 100 0.5818474908954627 1107 1 -1.0110578996130393 1107 2 1.6085307934924806 1107 3 -0.7613992431305725 1107 4 -0.10592064340056595 1107 5 1.86216641963631 1107 6 0.7603449996073408 1107 7 0.35443817858247684 1107 8 0.305298558820418 1107 9 0.2888553241996519 1107 10-11 0.2927153970926639 1107 12-13 0.26243650355577586 1107 14-15 0.36302887979626064 1107 16-17 0.2384531782881254 1107 18-19 0.22003070516313272 1107 20-21 0.1311444881403787 1107 22-23 0.1458206567391116 1107 24-25 0.051388675058710476 1107 26-27 0.09770779008182728 1107 28-29 0.07115202249439534 1107 30-31 0.06841703315183167 1107 32-33 0.06461217075840864 1107 34-35 0.10031857454876558 1107 36-37 0.21691594771272804 1107 38-39 0.19754208741733947 1107 40-41 0.19381540375902517 1107 42-43 0.28804348154146453 1107 44-45 0.33564329521212954 1107 46-47 0.26098261729649863 1107 48-49 0.2867794043863654 1107 50-51 0.3940151299796213 1107 52-53 0.42053147638349486 1107 54-55 0.5058147272484987 1107 56-57 0.5575407830991495 1107 58-59 0.6034238523643936 1107 60-61 0.5435005529841277 1107 62-63 0.5621460592114147 1107 64-65 0.457754010547589 1107 66-67 0.5256718351279517 1107 68-69 0.5695038825972532 1107 70-71 0.47787175429862216 1107 72-73 0.5043037980810787 1107 74-75 0.5089074136631915 1107 76-77 0.5137347996051993 1107 78-79 0.48083644038147 1107 80-81 0.4785132006017392 1107 82-83 0.490660172638421 1107 84-85 0.5222287551647895 1107 86-87 0.46474670650210115 1107 88-89 0.37965467380076134 1107 90-91 0.3974765454332605 1107 92-93 0.4302512453830687 1107 94-95 0.42154177804799176 1107 96-97 0.3817670589157096 1107 98-99 0.3852146459586727 1107 100 0.43502680335239674 1108 1 -1.3215184521328531 1108 2 0.32529211263018 1108 3 1.954268090354919 1108 4 0.8470793700253196 1108 5 0.7733743462772935 1108 6 0.4449121715347104 1108 7 0.21746553605554197 1108 8 0.131109594000705 1108 9 0.1656873977376847 1108 10-11 0.22022570145742293 1108 12-13 0.27970118140173383 1108 14-15 0.3204406381840812 1108 16-17 0.2716813422091988 1108 18-19 0.2186000655093352 1108 20-21 0.2160027779514735 1108 22-23 0.21163891951943725 1108 24-25 0.14583897467965556 1108 26-27 0.16192188367812577 1108 28-29 0.17587312216367224 1108 30-31 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-0.8515496518966827 2215 74-75 -0.8313366976247281 2215 76-77 -0.7718512054421005 2215 78-79 -0.7678285351312866 2215 80-81 -0.7687166131810486 2215 82-83 -0.7790228014139018 2215 84-85 -0.7322392449208373 2215 86-87 -0.7402144278792235 2215 88-89 -0.7759089490267215 2215 90-91 -0.7544490747159678 2215 92-93 -0.7606752143967235 2215 94-95 -0.7437896262757455 2215 96-97 -0.7632069910292287 2215 98-99 -0.7910050779860214 2215 100 -0.8108233285385786 2216 1 2.923617353534116 2216 2 -1.8994789138752957 2216 3 -2.143391584002881 2216 4 -0.8466297779868697 2216 5 -2.4940096290182616 2216 6 -1.028608252941467 2216 7 -0.5758164241113661 2216 8 -0.41477657288383796 2216 9 -0.4687189305514181 2216 10-11 -0.23933361309934753 2216 12-13 -0.29968867455643533 2216 14-15 -0.36290480318939444 2216 16-17 -0.42513346750907033 2216 18-19 -0.39940900843807015 2216 20-21 -0.3719203120473509 2216 22-23 -0.4585956391880259 2216 24-25 -0.46819872955499164 2216 26-27 -0.49132664615621735 2216 28-29 -0.6660053508876729 2216 30-31 -0.5702926683292731 2216 32-33 -0.5545480266145262 2216 34-35 -0.7026419387692258 2216 36-37 -0.6311238905569496 2216 38-39 -0.6006754195519193 2216 40-41 -0.5701911514156492 2216 42-43 -0.6313084762615659 2216 44-45 -0.47658258131769315 2216 46-47 -0.49303644907696054 2216 48-49 -0.5606019125997506 2216 50-51 -0.6715687716597003 2216 52-53 -0.6530499359183537 2216 54-55 -0.5016133686381679 2216 56-57 -0.2755333954477166 2216 58-59 -0.199657418415363 2216 60-61 -0.2653180076191859 2216 62-63 -0.16419364301963668 2216 64-65 -0.14979562903472754 2216 66-67 -0.11475367062203645 2216 68-69 -0.082422914592307 2216 70-71 -0.10589068130076029 2216 72-73 -0.1501006438581669 2216 74-75 -0.17257139551905354 2216 76-77 -0.10557004464388342 2216 78-79 -0.0737401867799683 2216 80-81 -0.17630189734687818 2216 82-83 -0.08105625255364046 2216 84-85 -0.08652765264142559 2216 86-87 -0.11605172812638287 2216 88-89 -0.14575317999037907 2216 90-91 -0.14155744728822484 2216 92-93 -0.19072837785365238 2216 94-95 -0.16813414440595764 2216 96-97 -0.1766793509790645 2216 98-99 -0.24775252413960303 2216 100 -0.27258027162367426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1584.0 3 350.0 4 392.0 5 870.0 6 2250.0 7 3860.0 8 4696.0 9 6424.0 10 5548.0 11 7568.0 12 6429.0 13 9009.0 14 8850.0 15 9584.0 16 11544.0 17 13108.0 18 18624.0 19 31606.0 20 61209.0 21 72775.0 22 34067.0 23 21818.0 24 21400.0 25 22072.0 26 23736.0 27 26115.0 28 30181.0 29 34966.0 30 40636.0 31 48507.0 32 61275.0 33 83513.0 34 119889.0 35 221264.0 36 328276.0 37 523826.0 38 558134.0 39 148866.0 40 372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.576431510198725 42.25969464472487 23.878529235177577 20.285344609898832 2 14.933266083871862 42.05371931679829 24.664425695186228 18.348588904143615 3 17.956164784328546 25.021903894093338 18.55553151418001 38.466399807398105 4 34.30734426002203 26.29498097854139 20.74807452252082 18.649600238915767 5 17.394110005980657 41.339400334459626 21.048824248703873 20.217665410855844 6 38.147631011998755 23.26859959883498 22.488251732000432 16.095517657165825 7 18.61029167517424 22.80405559838971 40.71315814334779 17.872494583088265 8 36.943417112570394 23.084626539839164 22.33321511980262 17.638741227787825 9 36.40155219063894 24.362361167350365 22.624317526368536 16.611769115642165 10-11 16.61992089724451 43.7820381206258 22.365860338649387 17.23218064348031 12-13 16.55337340911697 24.257702195609998 31.89744525450129 27.291479140771745 14-15 16.875425921065613 33.72386335023749 22.273124299813386 27.127586428883514 16-17 26.80147707235239 23.896471611801495 32.033949503903145 17.26810181194297 18-19 17.02838610342173 24.079905744072914 32.137941857989105 26.75376629451625 20-21 26.896536749869437 34.0357451814019 22.309083560713443 16.75863450801522 22-23 26.42706650520552 24.36977014642352 32.16555887509985 17.03760447327111 24-25 16.8385524416681 34.02031774425728 22.240383849873133 26.90074596420149 26-27 17.243173595841064 27.980900448252843 37.76749705021632 17.008428905689772 28-29 25.08735548205408 24.20275385466897 33.768431501988616 16.941459161288332 30-31 23.010365333139315 25.30686315253774 34.104063967868264 17.57870754645468 32-33 19.139411845148146 26.937924183098154 35.08627746607583 18.83638650567787 34-35 18.911314330032116 26.7463192230057 35.347591586599535 18.994774860362647 36-37 19.034371949033844 26.820009043144637 35.48485768474927 18.660761323072247 38-39 19.61486260248294 26.55760928815519 35.040433217671996 18.78709489168987 40-41 18.71348125642572 27.67436527523881 34.82688701364052 18.78526645469495 42-43 18.73323439355835 27.11735181698986 35.27363602911207 18.875777760339727 44-45 18.840310026729462 27.43215451206826 34.65223318818845 19.07530227301383 46-47 19.2279196234334 26.936000515009756 34.94013583001326 18.895944031543586 48-49 18.784199866447914 27.352255624634076 34.73668412189122 19.12686038702678 50-51 19.18005647584768 27.819478415491737 34.09888339638267 18.901581712277917 52-53 19.391552495543653 27.698533762991207 33.719356259805245 19.190557481659894 54-55 19.751214482135218 28.537235167090575 31.86342870790833 19.848121642865877 56-57 20.088713477447182 29.45137747967483 30.207150483793 20.252758559084988 58-59 20.907758020077 30.041657889534218 28.917683385564413 20.13290070482437 60-61 20.69392612276507 30.295315430141706 28.65941666003223 20.35134178706099 62-63 19.893661151770555 30.462865016019773 28.493047939713385 21.150425892496287 64-65 20.150442272244366 30.3193708043561 29.172940808542457 20.35724611485708 66-67 20.323248614482818 29.84492949661225 29.49556470705201 20.336257181852915 68-69 20.455886481489173 29.938351199321346 29.351213415546972 20.254548903642515 70-71 19.363585839212583 30.604073681439804 29.626983616061754 20.40535686328586 72-73 19.37479739492248 30.159253780977384 29.761509777801987 20.70443904629814 74-75 19.654988256070236 30.42018736958067 29.115060799501446 20.809763574847647 76-77 20.199445485326045 30.00195986672906 29.113086976752246 20.685507671192642 78-79 20.172105998645048 30.39068301675558 28.466342079956664 20.970868904642703 80-81 20.311667909762658 30.844982755376233 28.238804707968946 20.604544626892167 82-83 20.12433120883236 31.003054855017716 28.093847797405537 20.778766138744395 84-85 20.054491916683173 31.18912751984641 27.624868579438967 21.13151198403145 86-87 20.10499799443317 31.356094580474654 27.656328506132695 20.88257891895948 88-89 20.928765999299863 30.756538662109794 27.557059614283595 20.75763572430675 90-91 20.372323314318212 31.3129156482489 27.48009050006447 20.834670537368417 92-93 19.959770576868063 31.39782484563992 27.440572940732356 21.20183163675966 94-95 20.0812130919923 31.652516910664648 27.121439665853075 21.144830331489977 96-97 20.006094878207193 31.014302012643064 27.44479849761252 21.534804611537222 98-99 20.5411214020535 31.104843176348425 26.61219543969971 21.74183998189837 100 20.07421930501872 31.796252694563794 26.432761324595944 21.69676667582155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 53984.0 1 29897.5 2 4368.5 3 2534.0 4 2175.5 5 2092.5 6 2074.0 7 2325.5 8 2262.0 9 2045.5 10 2091.5 11 3151.0 12 21764.5 13 24820.0 14 9266.5 15 8598.0 16 9483.0 17 10667.5 18 11967.5 19 13341.0 20 14833.5 21 16460.0 22 18674.0 23 21400.0 24 23992.5 25 26725.0 26 30259.0 27 33975.0 28 36927.0 29 39921.0 30 43345.5 31 46727.0 32 50120.5 33 54001.5 34 58177.5 35 61620.0 36 64767.0 37 67430.5 38 69039.0 39 70160.5 40 70757.5 41 71151.0 42 71888.0 43 73001.5 44 74916.0 45 76821.5 46 78199.5 47 80398.5 48 81745.5 49 83932.5 50 94735.5 51 94308.0 52 79227.5 53 72299.0 54 70553.5 55 68923.5 56 62600.0 57 53385.5 58 46615.5 59 39103.0 60 31365.5 61 25295.0 62 20322.5 63 15740.0 64 11765.5 65 8788.0 66 6694.0 67 5144.5 68 3879.0 69 2954.0 70 2248.5 71 1744.0 72 1321.0 73 902.5 74 668.0 75 496.5 76 331.5 77 212.5 78 128.5 79 74.0 80 40.0 81 23.0 82 9.5 83 2.5 84 1.5 85 1.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13678994268230946 2 0.0053710336725718835 3 0.003390226928077288 4 0.0 5 0.0023998235558299903 6 0.01287524383921487 7 0.0030854874289242733 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.9046218697063416E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0010284958096414244 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.3332353087944392E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.5710924872952193E-4 74-75 2.6664706175888784E-4 76-77 5.523403422148391E-4 78-79 3.2378571785007804E-4 80-81 0.0010284958096414244 82-83 0.0010475420283384879 84-85 0.0012570504340061854 86-87 0.0 88-89 0.0 90-91 5.713865609119024E-5 92-93 0.0 94-95 1.9046218697063416E-5 96-97 0.0014475126209768196 98-99 5.713865609119024E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2625193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.6717369312377 #Duplication Level Percentage of deduplicated Percentage of total 1 90.70452540038836 61.38132781367849 2 6.418885723247477 8.687542921105615 3 1.276910380905193 2.5923223014414827 4 0.489525165377585 1.3250807285061024 5 0.26189554102655804 0.8861463077906704 6 0.13683767550105874 0.555602590727383 7 0.08977870378697042 0.42528365772895627 8 0.06499989124192557 0.35189244325461183 9 0.047982715429751674 0.2922366326227736 >10 0.4644651659503997 6.100687550592821 >50 0.02509280505438774 1.1719819064969141 >100 0.01588331927974656 2.2354760454194746 >500 0.002201456133627944 1.0421851382484633 >1k 6.209235248684511E-4 1.0036422798925575 >5k 1.6934277950957755E-4 0.6957578018559427 >10k+ 2.2579037267943675E-4 11.252833880637478 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 213477 8.131859257586013 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52682 2.0067857867973897 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 16825 0.6409052591561839 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 11600 0.4418722737718712 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 7161 0.27277994417934226 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5938 0.22619289324632513 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5115 0.19484281727095873 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4624 0.17613943051044248 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4208 0.1602929765544857 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 4062 0.1547314806949432 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.475714357001562E-4 0.0 0.0 0.0 0.0 2 6.475714357001562E-4 0.0 0.0 0.0 0.0 3 6.475714357001562E-4 0.0 0.0 0.0 0.0 4 6.475714357001562E-4 0.0 0.0 0.0 0.0 5 6.475714357001562E-4 0.0 0.0 0.0 0.0 6 6.475714357001562E-4 0.0 0.0 0.0 0.0 7 6.475714357001562E-4 0.0 0.0 0.0 0.0 8 6.475714357001562E-4 0.0 0.0 0.0 0.0 9 6.475714357001562E-4 0.0 0.0 0.0 0.0 10-11 6.475714357001562E-4 0.0 0.0 0.0 0.0 12-13 6.475714357001562E-4 0.0 0.0 0.0 0.0 14-15 6.475714357001562E-4 0.0 0.0 0.0 0.0 16-17 6.666176543972196E-4 0.0 0.0 0.0 0.0 18-19 7.618487478825367E-4 0.0 0.0 0.0 0.0 20-21 7.618487478825367E-4 0.0 0.0 0.0 0.0 22-23 7.999411852766636E-4 0.0 0.0 0.0 0.0 24-25 8.380336226707903E-4 0.0 0.0 0.0 0.0 26-27 8.761260600649171E-4 0.0 0.0 0.0 0.0 28-29 9.904033722472976E-4 0.0 0.0 0.0 0.0 30-31 0.0010856344657326146 0.0 0.0 0.0 0.0 32-33 0.0012951428714003121 0.0 0.0 0.0 0.0 34-35 0.0017332059014327707 0.0 0.0 0.0 0.0 36-37 0.002380777337132927 0.0 0.0 0.0 0.0 38-39 0.003980659707686254 0.0 0.0 0.0 0.0 40-41 0.006723315200063386 0.0 0.0 0.0 0.0 42-43 0.010475420283384879 0.0 0.0 0.0 0.0 44-45 0.014817958146315337 0.0 0.0 0.0 0.0 46-47 0.01824627751178675 0.0 0.0 0.0 0.0 48-49 0.022988785967355544 0.0 0.0 0.0 0.0 50-51 0.0288931137634452 0.0 0.0 0.0 0.0 52-53 0.036206861743117554 0.0 0.0 0.0 0.0 54-55 0.04563473999816395 0.0 0.0 0.0 0.0 56-57 0.05531021909627216 0.0 0.0 0.0 0.0 58-59 0.06749979906239276 0.0 0.0 0.0 0.0 60-61 0.08187969417867563 0.0 0.0 0.0 0.0 62-63 0.1009449590944361 0.0 0.0 0.0 0.0 64-65 0.12406706859267108 0.0 0.0 0.0 0.0 66-67 0.1501413419889509 0.0 0.0 0.0 0.0 68-69 0.184100749925815 0.0 0.0 0.0 0.0 70-71 0.22988785967355543 0.0 0.0 0.0 3.809243739412683E-5 72-73 0.2824935157148446 0.0 0.0 0.0 3.809243739412683E-5 74-75 0.33951789449385245 0.0 0.0 0.0 3.809243739412683E-5 76-77 0.4071891095245188 0.0 0.0 0.0 3.809243739412683E-5 78-79 0.4845929423093845 0.0 0.0 0.0 3.809243739412683E-5 80-81 0.5789479097346366 0.0 0.0 0.0 3.809243739412683E-5 82-83 0.695872646315909 0.0 0.0 3.809243739412683E-5 3.809243739412683E-5 84-85 0.8376717445155462 0.0 0.0 3.809243739412683E-5 3.809243739412683E-5 86-87 1.0003264521884676 0.0 0.0 7.618487478825366E-5 3.809243739412683E-5 88 1.136525962091168 0.0 0.0 7.618487478825366E-5 3.809243739412683E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3650 0.0 87.44641 1 AACGAGT 51515 0.0 78.023895 1 ACGAGTG 56325 0.0 77.296074 2 GTGGAAT 62625 0.0 76.54527 6 CGAGTGG 58710 0.0 76.5233 3 TGGAATC 62795 0.0 76.24554 7 GAATCAC 63615 0.0 75.40905 9 GGAATCA 63605 0.0 75.3618 8 AACGTGT 2160 0.0 75.1918 1 GAGTGGA 61430 0.0 75.12036 4 AGTGGAA 61685 0.0 74.77171 5 ATCGAGT 1370 0.0 74.566605 1 CGTGTGG 2455 0.0 72.763954 3 ACGTGTG 2370 0.0 71.21097 2 TCGAGTG 1545 0.0 70.59277 2 GTGTGGA 3590 0.0 51.196663 4 TGAGTGG 2705 0.0 51.093334 3 AGCGAGT 875 0.0 50.03571 1 ATGAGTG 2585 0.0 50.011845 2 GCGAGTG 880 0.0 49.682224 2 >>END_MODULE