FastQCFastQC Report
Thu 26 May 2016
SRR2132782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778582
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3217838.515972393876856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA309510.8191168009586666No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT252840.6691399048637823No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107550.2846305836422235No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89400.23659669156313134No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG86590.22916003940102397No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74260.19652875073241763No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60870.161092176906575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT55550.089.317351
AACGAGT731450.085.137651
ACGAGTG801700.084.011772
CGAGTGG836700.083.122753
GTGGAAT893950.082.5131456
TGGAATC900550.081.817137
GAGTGGA874250.081.6002044
GGAATCA906650.081.2863168
AGTGGAA878350.081.122985
GAATCAC910600.081.011139
ATCGAGT20900.079.7326661
AACGTGT31250.073.811721
CGTGTGG34950.071.1561663
ACGTGTG34350.069.528692
TCGAGTG24900.068.916082
GCGAGTG12200.050.4822272
ATGAGTG38500.050.1889572
TGAGTGG42000.050.145593
GTGTGGA53700.048.9335944
TGTGGAA56900.045.768545