FastQCFastQC Report
Thu 26 May 2016
SRR2132781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119125
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1667337.868011561375568No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA842023.9734324308381996No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130960.6179909160620539No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG95490.45061051141390907No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67860.32022650858255175No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56230.2653453666017814No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45400.21423936766354038No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38630.1822922196661358No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38600.18215065180204093No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC23990.11320710198784877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT28400.087.017531
AACGAGT421950.075.2465741
ACGAGTG460700.074.3978352
GTGGAAT502600.074.275846
CGAGTGG477400.074.007423
TGGAATC505000.073.869337
GGAATCA510300.073.107888
GAATCAC510850.073.047579
GAGTGGA496650.072.9544754
AGTGGAA498700.072.616885
AACGTGT13750.072.576981
TCGAGTG11200.071.354662
CGTGTGG15250.071.205423
ATCGAGT10850.070.7169651
ACGTGTG15900.065.636782
AGCGAGT5800.057.6230351
GCGAGTG6100.056.2580222
TGAGTGG20850.049.149753
ATGAGTG19350.047.8604352
GTGTGGA26450.044.4298634