##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132781_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2119125 Sequences flagged as poor quality 0 Sequence length 100 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.924319235533535 31.0 25.0 33.0 16.0 34.0 2 28.19157907155076 31.0 25.0 34.0 16.0 34.0 3 29.267861027546747 31.0 28.0 34.0 16.0 34.0 4 34.76955677461216 35.0 35.0 37.0 32.0 37.0 5 33.85197616940955 35.0 35.0 37.0 28.0 37.0 6 35.27303202972925 37.0 35.0 37.0 32.0 37.0 7 35.658021117206395 37.0 35.0 37.0 33.0 37.0 8 35.927961776676696 37.0 35.0 37.0 35.0 37.0 9 37.900123871881085 39.0 38.0 39.0 35.0 39.0 10-11 37.89226685542382 39.0 38.0 39.0 35.0 39.0 12-13 37.86572429658467 39.0 38.0 39.0 35.0 39.0 14-15 39.281944434613344 41.0 39.0 41.0 36.0 41.0 16-17 39.22244228160207 40.5 39.0 41.0 36.0 41.0 18-19 38.990239131717104 40.0 39.0 41.0 35.5 41.0 20-21 38.87239167109066 40.0 39.0 41.0 35.0 41.0 22-23 38.70852592461512 40.0 39.0 41.0 35.0 41.0 24-25 38.53802158909927 40.0 38.0 41.0 35.0 41.0 26-27 38.32792827228219 40.0 38.0 41.0 34.5 41.0 28-29 38.1626862502212 40.0 38.0 41.0 34.0 41.0 30-31 38.03414003421223 40.0 38.0 41.0 34.0 41.0 32-33 37.89097504866395 40.0 38.0 41.0 34.0 41.0 34-35 37.80323576948033 40.0 38.0 41.0 33.5 41.0 36-37 37.62407432312865 40.0 37.5 41.0 33.0 41.0 38-39 37.112648852710436 40.0 36.0 41.0 33.0 41.0 40-41 36.85523458974812 40.0 35.0 41.0 33.0 41.0 42-43 36.69793310918422 40.0 35.0 41.0 33.0 41.0 44-45 36.54617353860674 39.0 35.0 41.0 32.0 41.0 46-47 36.42093623547454 39.0 35.0 41.0 31.5 41.0 48-49 36.26456910281367 39.0 35.0 41.0 31.0 41.0 50-51 35.98832537014098 39.0 35.0 41.0 30.5 41.0 52-53 35.52517949625435 39.0 35.0 41.0 29.0 41.0 54-55 34.62775084055919 39.0 35.0 41.0 22.0 41.0 56-57 33.87152928685188 39.0 35.0 41.0 10.0 41.0 58-59 33.52271173243673 38.0 34.0 40.5 3.5 41.0 60-61 33.27875585442105 38.0 34.0 40.0 2.0 41.0 62-63 33.015151064708306 37.0 34.0 40.0 2.0 41.0 64-65 32.752637291334864 37.0 34.0 40.0 2.0 41.0 66-67 32.459592284551405 36.0 33.5 40.0 2.0 41.0 68-69 32.15293835899251 36.0 33.0 39.0 2.0 41.0 70-71 31.82988167286026 35.0 33.0 39.0 2.0 41.0 72-73 31.505497316109242 35.0 33.0 39.0 2.0 40.5 74-75 31.173337108476375 35.0 33.0 37.5 2.0 40.0 76-77 30.837050905444464 35.0 33.0 37.0 2.0 39.5 78-79 30.49167203444818 35.0 32.5 36.5 2.0 39.0 80-81 30.16876375862679 35.0 32.0 36.0 2.0 39.0 82-83 29.856961481743646 35.0 32.0 36.0 2.0 37.5 84-85 29.55782221435734 35.0 32.0 35.5 2.0 37.0 86-87 29.327122987081932 35.0 32.0 35.0 2.0 37.0 88-89 29.09726915590161 35.0 31.0 35.0 2.0 36.0 90-91 28.88365245089365 35.0 31.0 35.0 2.0 36.0 92-93 28.689485990680115 35.0 31.0 35.0 2.0 36.0 94-95 28.4848343066124 35.0 31.0 35.0 2.0 35.5 96-97 28.28371426886097 35.0 31.0 35.0 2.0 35.0 98-99 28.104234766707958 35.0 30.5 35.0 2.0 35.0 100 27.993171238128944 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06615705191189392 1101 2 2.229236702292315 1101 3 1.036336818294707 1101 4 0.5908711557184958 1101 5 2.182191729409574 1101 6 0.9774678399112489 1101 7 0.5857515580099459 1101 8 0.3850279458667458 1101 9 0.35906037218812514 1101 10-11 0.4773218601446132 1101 12-13 0.40216222728698625 1101 14-15 0.49979938343079056 1101 16-17 0.4990106611242169 1101 18-19 0.4761616798176007 1101 20-21 0.528445274967325 1101 22-23 0.4825435703361478 1101 24-25 0.37842503074539735 1101 26-27 0.3417256542469218 1101 28-29 0.38397132983786264 1101 30-31 0.4028834102335992 1101 32-33 0.34796325000441897 1101 34-35 0.41565803663174705 1101 36-37 0.38529599911734635 1101 38-39 0.4655238403041224 1101 40-41 0.4202865000747167 1101 42-43 0.4673731422326952 1101 44-45 0.49651221997006445 1101 46-47 0.4209566894500867 1101 48-49 0.35601925514180977 1101 50-51 0.39263093885696065 1101 52-53 0.5469650859746054 1101 54-55 0.5465559705216734 1101 56-57 0.48573710102717627 1101 58-59 0.4132651414530102 1101 60-61 0.2943277166093523 1101 62-63 0.31817811612410907 1101 64-65 0.3327449030608136 1101 66-67 0.25087609994971416 1101 68-69 0.3491209912625237 1101 70-71 0.24729949676928697 1101 72-73 0.3052941471518196 1101 74-75 0.3375675804727969 1101 76-77 0.2568665329241391 1101 78-79 0.3187659537674854 1101 80-81 0.3585399489941601 1101 82-83 0.36089572501129297 1101 84-85 0.34268842101967323 1101 86-87 0.38951489724236055 1101 88-89 0.3325743923440214 1101 90-91 0.35589513669637896 1101 92-93 0.3457833488753792 1101 94-95 0.3317519230080386 1101 96-97 0.3979269595305155 1101 98-99 0.48924217543307336 1101 100 0.4734406543474883 1102 1 1.7519580844553744 1102 2 3.2127689575329654 1102 3 3.2280610975853357 1102 4 1.4204920867157256 1102 5 2.6659789178423523 1102 6 1.1971399710587889 1102 7 0.7013626930818333 1102 8 0.5202198289988829 1102 9 0.4624475247509068 1102 10-11 0.4762233414900834 1102 12-13 0.45420233335969584 1102 14-15 0.6004561459927658 1102 16-17 0.47038384146916457 1102 18-19 0.3675086836053012 1102 20-21 0.26255038259866836 1102 22-23 0.17727025377061523 1102 24-25 0.25994963372004776 1102 26-27 0.29292922074946404 1102 28-29 0.31977065428625906 1102 30-31 0.3418066270890847 1102 32-33 0.345707283414761 1102 34-35 0.38777978964974125 1102 36-37 0.32766615186373826 1102 38-39 0.3737820530388234 1102 40-41 0.39995226856805033 1102 42-43 0.48771762230990134 1102 44-45 0.553505161267104 1102 46-47 0.5581385033616684 1102 48-49 0.4988166451725178 1102 50-51 0.5804974853876033 1102 52-53 0.6300265475061622 1102 54-55 0.6169128342882786 1102 56-57 0.42950255394993775 1102 58-59 0.46353288535831183 1102 60-61 0.4431139015362575 1102 62-63 0.49382217472885515 1102 64-65 0.4479828193556088 1102 66-67 0.45206642871531955 1102 68-69 0.37563660538675947 1102 70-71 0.36702906261422186 1102 72-73 0.3704891475574925 1102 74-75 0.4218121113658313 1102 76-77 0.4214696783812464 1102 78-79 0.4200600493097859 1102 80-81 0.45249927070960183 1102 82-83 0.44197344760810964 1102 84-85 0.4389459846782451 1102 86-87 0.41472616725376454 1102 88-89 0.34045020529053005 1102 90-91 0.3595363684037487 1102 92-93 0.3258449684057396 1102 94-95 0.3023605172884807 1102 96-97 0.2868130253178016 1102 98-99 0.32999476212957646 1102 100 0.31293779115309306 1103 1 1.3725909094413744 1103 2 3.004106751678865 1103 3 3.2056878199508425 1103 4 1.4186020319842498 1103 5 2.581119384388302 1103 6 1.2279530951672086 1103 7 0.7582298783145518 1103 8 0.5019148287740691 1103 9 0.45505693660217617 1103 10-11 0.5286504922243935 1103 12-13 0.42790956290079407 1103 14-15 0.5365380429144722 1103 16-17 0.3578765350832853 1103 18-19 0.35531877070968676 1103 20-21 0.27010855778025444 1103 22-23 0.15951175077243818 1103 24-25 0.20422020094083848 1103 26-27 0.1726340223223346 1103 28-29 0.20492223880120974 1103 30-31 0.21354818335949233 1103 32-33 0.19853439490918845 1103 34-35 0.2719125676296201 1103 36-37 0.1250873553021421 1103 38-39 0.26266163153700006 1103 40-41 0.2712243767720466 1103 42-43 0.2699891623529851 1103 44-45 0.32518724457829506 1103 46-47 0.2799722325001426 1103 48-49 0.2177288418297607 1103 50-51 0.3387568692395675 1103 52-53 0.4190318162789737 1103 54-55 0.45733278286767387 1103 56-57 0.40578510568732185 1103 58-59 0.343280809935969 1103 60-61 0.30187686398450353 1103 62-63 0.2943067465031106 1103 64-65 0.35052278477545684 1103 66-67 0.3366596622866709 1103 68-69 0.2828314028312633 1103 70-71 0.27505545961563627 1103 72-73 0.28831223287564356 1103 74-75 0.3773721177423148 1103 76-77 0.372896007530354 1103 78-79 0.2906842905697111 1103 80-81 0.29010375713709635 1103 82-83 0.34527082466667025 1103 84-85 0.38464083730384147 1103 86-87 0.3560982963039514 1103 88-89 0.39533761541687085 1103 90-91 0.40061154140670396 1103 92-93 0.458214812549798 1103 94-95 0.3933065256514823 1103 96-97 0.4641378866497483 1103 98-99 0.4274658329289629 1103 100 0.42805945525145717 1104 1 6.101968559684217 1104 2 5.082982762466873 1104 3 4.101729522725254 1104 4 1.8055855997924297 1104 5 2.8128721854994154 1104 6 1.2831212794700022 1104 7 0.6751996670166278 1104 8 0.480426246139821 1104 9 0.40159369121096233 1104 10-11 0.48699959532771686 1104 12-13 0.39895014813244956 1104 14-15 0.4697089077514036 1104 16-17 0.3771817136587714 1104 18-19 0.26494508793968663 1104 20-21 0.2767381743101609 1104 22-23 0.21555052048726964 1104 24-25 0.1812509721865041 1104 26-27 0.20958798505051135 1104 28-29 0.22107233930163517 1104 30-31 0.2471706531054565 1104 32-33 0.30749097072681764 1104 34-35 0.35620634726900846 1104 36-37 0.3571929078035083 1104 38-39 0.48073445454411257 1104 40-41 0.501522607760684 1104 42-43 0.5204011239032411 1104 44-45 0.5283698102839267 1104 46-47 0.4395665527165278 1104 48-49 0.5343583877780205 1104 50-51 0.5134363305290677 1104 52-53 0.426573975089255 1104 54-55 0.47496568614964474 1104 56-57 0.33513405272677943 1104 58-59 0.3761302537917999 1104 60-61 0.38153609578953507 1104 62-63 0.44843765506929145 1104 64-65 0.34265171548702256 1104 66-67 0.42242365764710854 1104 68-69 0.4372314235352093 1104 70-71 0.4155651296934906 1104 72-73 0.42925856808043505 1104 74-75 0.4624699399847856 1104 76-77 0.5352298759771408 1104 78-79 0.5542110280742101 1104 80-81 0.5450309051130908 1104 82-83 0.5008057982652154 1104 84-85 0.5516314868040055 1104 86-87 0.5210827408930463 1104 88-89 0.4909595534477518 1104 90-91 0.47650237983041066 1104 92-93 0.4506210899246952 1104 94-95 0.5150017556653061 1104 96-97 0.4765417877758402 1104 98-99 0.5196803634943485 1104 100 0.4785454883197424 1105 1 1.551979118816046 1105 2 3.02680610164483 1105 3 3.1800728896727186 1105 4 1.3399214935547832 1105 5 2.549808552366322 1105 6 1.164189564782525 1105 7 0.6655961712739398 1105 8 0.4447259831550596 1105 9 0.3055890787726838 1105 10-11 0.33403254859905473 1105 12-13 0.32800756963096234 1105 14-15 0.3915721793940463 1105 16-17 0.26771809753120124 1105 18-19 0.2365508815836037 1105 20-21 0.20176437189362417 1105 22-23 0.19054678897787625 1105 24-25 0.27502946393582306 1105 26-27 0.28775243979342946 1105 28-29 0.26542392773855994 1105 30-31 0.2644530868536492 1105 32-33 0.3707948529907199 1105 34-35 0.35930207523373525 1105 36-37 0.34089010116932883 1105 38-39 0.3720693583204593 1105 40-41 0.3529342504704758 1105 42-43 0.37893043785541636 1105 44-45 0.5018176756680148 1105 46-47 0.45360222671646255 1105 48-49 0.4189846230077521 1105 50-51 0.4430403469116655 1105 52-53 0.44143242958047324 1105 54-55 0.4506492994398812 1105 56-57 0.39570704004919577 1105 58-59 0.337966462941786 1105 60-61 0.38921776792471263 1105 62-63 0.4832249112831235 1105 64-65 0.5715338369128844 1105 66-67 0.4851745525547173 1105 68-69 0.510066481886156 1105 70-71 0.5760973207202724 1105 72-73 0.5208510268732525 1105 74-75 0.5508732482971084 1105 76-77 0.5660516044061303 1105 78-79 0.6326872891279898 1105 80-81 0.5868038241669851 1105 82-83 0.5444863311540225 1105 84-85 0.5150490241433623 1105 86-87 0.5557691423794289 1105 88-89 0.49859066343817204 1105 90-91 0.5106059495871484 1105 92-93 0.5240900042564789 1105 94-95 0.5875850430318899 1105 96-97 0.5743233919659723 1105 98-99 0.5577196551714572 1105 100 0.5436954857550518 1106 1 -0.5452850790909558 1106 2 1.8624865973916442 1106 3 2.676917151537779 1106 4 1.1915168891033616 1106 5 2.3864642355124133 1106 6 1.0764742290338276 1106 7 0.5754586836075646 1106 8 0.4139176937124134 1106 9 0.2545148142922571 1106 10-11 0.36211467357024674 1106 12-13 0.33676435919932146 1106 14-15 0.3873948533754543 1106 16-17 0.3649953528609373 1106 18-19 0.3073894207222523 1106 20-21 0.2927678853507345 1106 22-23 0.26334499078423335 1106 24-25 0.2355734688105997 1106 26-27 0.21686843284346224 1106 28-29 0.255784984907919 1106 30-31 0.29293427930723226 1106 32-33 0.21428403728653223 1106 34-35 0.2762091837179881 1106 36-37 0.2637836105032534 1106 38-39 0.3734609453763582 1106 40-41 0.3504470446073782 1106 42-43 0.3518248992930779 1106 44-45 0.41639477255991864 1106 46-47 0.4510304196352237 1106 48-49 0.43109650957605794 1106 50-51 0.48706985979595885 1106 52-53 0.5474245819139654 1106 54-55 0.5059053589972748 1106 56-57 0.4796197900395782 1106 58-59 0.5159026440109287 1106 60-61 0.5088722203309928 1106 62-63 0.46562649775997755 1106 64-65 0.464881507503641 1106 66-67 0.44739546471672753 1106 68-69 0.5348607011785802 1106 70-71 0.4619479710071275 1106 72-73 0.47805345850734327 1106 74-75 0.538452880116651 1106 76-77 0.5139554411510368 1106 78-79 0.5163035268103897 1106 80-81 0.5259144970944796 1106 82-83 0.5691873220025556 1106 84-85 0.5332308246609188 1106 86-87 0.5404972429246904 1106 88-89 0.5922993562230516 1106 90-91 0.6644161716060815 1106 92-93 0.6890437650468257 1106 94-95 0.7014462744945256 1106 96-97 0.6921337554403202 1106 98-99 0.5661603074363555 1106 100 0.5637498994553951 1107 1 -0.9373956618999415 1107 2 1.6558556148445973 1107 3 -0.6638554801524847 1107 4 -0.1262337911590592 1107 5 1.8392851383802977 1107 6 0.7467000883608463 1107 7 0.27935763464087415 1107 8 0.2028732417659853 1107 9 0.15076269419187582 1107 10-11 0.25299610657507543 1107 12-13 0.22345928031328555 1107 14-15 0.28999937381586705 1107 16-17 0.21069759925218534 1107 18-19 0.1797384309353376 1107 20-21 0.1459949120040065 1107 22-23 0.11596984791895437 1107 24-25 0.051572956325898645 1107 26-27 -0.04987090361087354 1107 28-29 -0.04477488428645415 1107 30-31 0.0010181152958637085 1107 32-33 -0.0029285340444218377 1107 34-35 0.012641354741454336 1107 36-37 0.03256536116724362 1107 38-39 0.014997423304947688 1107 40-41 -0.06015915157664864 1107 42-43 -0.03181199309733529 1107 44-45 0.04911305646830755 1107 46-47 0.04494832446763297 1107 48-49 0.025982237690726606 1107 50-51 0.02642258348942761 1107 52-53 0.114101281349825 1107 54-55 -0.0172270206806715 1107 56-57 -0.008222324000470849 1107 58-59 0.04757358816230095 1107 60-61 -0.07623379485644932 1107 62-63 -0.024425604850840443 1107 64-65 -0.11794496761847739 1107 66-67 -0.18879266597500788 1107 68-69 -0.1439610720998168 1107 70-71 -0.07022610296149168 1107 72-73 -0.10219106990505367 1107 74-75 -0.10812974177132517 1107 76-77 -0.18287915237945285 1107 78-79 -0.13581555673681933 1107 80-81 -0.13952024041980948 1107 82-83 -0.06583856660930465 1107 84-85 -0.07064026510118282 1107 86-87 0.00601170028067699 1107 88-89 0.06272919259400567 1107 90-91 -0.003366437266411282 1107 92-93 -0.0071756434807568326 1107 94-95 -0.025865499231276345 1107 96-97 -0.04885444520963844 1107 98-99 -0.06088281855759803 1107 100 -0.06546132151905582 1108 1 -1.637540433420785 1108 2 0.13542055786400908 1108 3 1.9763705861454461 1108 4 0.8464173837175153 1108 5 0.4746873433619214 1108 6 0.4136532046606334 1108 7 0.2172914851921206 1108 8 0.21059624558943568 1108 9 0.18384484274972834 1108 10-11 0.30157623204372186 1108 12-13 0.28112381871485326 1108 14-15 0.3735279012296502 1108 16-17 0.3240035868538058 1108 18-19 0.32073382794339267 1108 20-21 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-0.29605144467688405 2215 46-47 -0.3933737740888006 2215 48-49 -0.4080039939296398 2215 50-51 -0.3649803226307782 2215 52-53 -0.4382928289777652 2215 54-55 -0.5016480628299291 2215 56-57 -0.317393596724024 2215 58-59 -0.38571254452750026 2215 60-61 -0.3987428247133735 2215 62-63 -0.42105703312711995 2215 64-65 -0.40082653665907486 2215 66-67 -0.46716042335966534 2215 68-69 -0.42703603503575494 2215 70-71 -0.40694586143317935 2215 72-73 -0.4559532698676385 2215 74-75 -0.4711027680693576 2215 76-77 -0.5175445427829111 2215 78-79 -0.5927479554260593 2215 80-81 -0.5417134183989631 2215 82-83 -0.5563430008018635 2215 84-85 -0.559539055319064 2215 86-87 -0.5271051429502087 2215 88-89 -0.5170542118272472 2215 90-91 -0.6567402672938378 2215 92-93 -0.6541970703703228 2215 94-95 -0.7120495621969063 2215 96-97 -0.7165301167601719 2215 98-99 -0.6972270503845763 2215 100 -0.7708236744843191 2216 1 2.9296506852805457 2216 2 -1.8231809984941556 2216 3 -2.1189432644322217 2216 4 -0.7972634486147783 2216 5 -2.4449276595341978 2216 6 -0.9855734354169954 2216 7 -0.5255766948862401 2216 8 -0.3423487085022785 2216 9 -0.34776192723278143 2216 10-11 -0.19131712160912429 2216 12-13 -0.19764088550103054 2216 14-15 -0.3267249969647139 2216 16-17 -0.33882163340341975 2216 18-19 -0.21927633540615687 2216 20-21 -0.14588995651772052 2216 22-23 -0.14232938217842417 2216 24-25 -0.08687795502504514 2216 26-27 -0.05994320116321461 2216 28-29 -0.18002941671441874 2216 30-31 -0.17975833200885916 2216 32-33 -0.24749083683094142 2216 34-35 -0.35113394606674575 2216 36-37 -0.23975075013002822 2216 38-39 -0.3282933748245682 2216 40-41 -0.16414979911568395 2216 42-43 -0.2250760317941456 2216 44-45 -0.3279293513470165 2216 46-47 -0.34287436800213555 2216 48-49 -0.2941732652178928 2216 50-51 -0.3244942605925374 2216 52-53 -0.417685942708232 2216 54-55 -0.07390039943965121 2216 56-57 0.0676432180899198 2216 58-59 0.1499760123628704 2216 60-61 0.09985555339834917 2216 62-63 0.21057377128555288 2216 64-65 0.148133331580226 2216 66-67 0.1330016363846127 2216 68-69 0.15870302168532646 2216 70-71 0.14231641981737653 2216 72-73 0.20174539174459838 2216 74-75 0.07653055076185211 2216 76-77 0.10536180336389833 2216 78-79 0.07173032862765538 2216 80-81 0.08710849481640182 2216 82-83 -0.03733875062944847 2216 84-85 0.02821782907789583 2216 86-87 -0.05102532425874173 2216 88-89 -0.1187514358524453 2216 90-91 -0.1342248500475307 2216 92-93 -0.22252347294548258 2216 94-95 -0.30568260910268563 2216 96-97 -0.32052193453921873 2216 98-99 -0.44317235091100926 2216 100 -0.507401984635063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 989.0 3 265.0 4 355.0 5 1003.0 6 3162.0 7 5430.0 8 6748.0 9 9222.0 10 6287.0 11 4594.0 12 4340.0 13 6020.0 14 6202.0 15 7061.0 16 8594.0 17 9691.0 18 13651.0 19 23359.0 20 46494.0 21 56696.0 22 26044.0 23 16219.0 24 15629.0 25 16037.0 26 16897.0 27 18685.0 28 21922.0 29 25497.0 30 29342.0 31 35952.0 32 46425.0 33 64519.0 34 94013.0 35 200842.0 36 245558.0 37 400424.0 38 479517.0 39 144910.0 40 530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.96016792490634 43.70220169890709 24.67847786392866 18.65915251225791 2 14.118690819170046 43.4024921721444 24.695439197559267 17.783377811126293 3 17.351329464929535 26.9802760484897 19.165729138745323 36.502665347835446 4 32.664755500501386 28.3344776735681 21.44243496726243 17.558331858668083 5 16.3525345600373 42.16371741135009 21.71046111824738 19.773286910365226 6 36.25238453471722 25.122447804529035 23.402515123684317 15.222652537069429 7 17.86235406265042 24.61520674261229 40.365303483620096 17.157135711117196 8 35.45826697339704 24.96256709726892 22.886332802453843 16.6928331268802 9 34.879631923553355 26.72272754084823 22.82668554238188 15.57095499321654 10-11 15.690650622308736 45.15141862797145 23.003173479620127 16.154757270099687 12-13 15.550805167227038 26.386503863622956 32.12809532236182 25.93459564678818 14-15 15.848003303250163 35.48952987671798 22.913561021648086 25.748905798383763 16-17 25.453760396390017 25.975839084527813 32.2577714858727 16.31262903320946 18-19 16.066277355040405 26.19184805049254 32.42291039933935 25.31896419512771 20-21 25.5052203149885 35.76565799563499 22.97771485872707 15.751406830649442 22-23 25.113171710021824 26.46665486934466 32.432395446233706 15.987777974399812 24-25 15.880351560195836 35.69527517253584 22.794502447944318 25.62987081932401 26-27 16.37709732889628 29.91618234704529 37.51165575964408 16.195064564414352 28-29 23.871385595469828 26.32159499793547 33.67816905562437 16.128850350970332 30-31 21.601934760809296 27.520509644310742 34.03574588568395 16.841809709196014 32-33 18.004034684126704 29.135610216480856 34.868141331917656 17.992213767474784 34-35 17.988131894060047 28.858278770719046 35.06619477378635 18.087394561434557 36-37 18.074606264377987 28.884091311272343 35.299239072730494 17.742063351619183 38-39 18.47234117855247 28.66834188639179 35.032053323895475 17.827263611160266 40-41 17.67807467704831 29.627063056686133 34.985265144812125 17.70959712145343 42-43 17.68407909076902 28.96645973226018 35.310256501610695 18.0392046753601 44-45 17.709502742877365 29.488420928449244 34.68587270689554 18.116203621777856 46-47 18.18722350026544 29.01294166224267 34.96721524214003 17.832619595351858 48-49 17.7461216303899 29.274912994750192 34.87823983955642 18.100725535303486 50-51 18.11886981655164 29.721488822037394 34.246375272813076 17.913266088597886 52-53 18.34622585527736 29.559074569780652 33.97387361985916 18.12082595508282 54-55 18.753259010204683 30.389972276293282 32.08033976287383 18.77642895062821 56-57 19.179897363298533 31.21052321123105 30.513325075207927 19.09625435026249 58-59 19.868153129239662 31.671892880316165 29.42426709137026 19.035686899073912 60-61 19.549696218958296 31.983884858137202 29.23782221435734 19.228596708547162 62-63 18.901277131493956 32.116147993227386 29.099665710925837 19.882909164352817 64-65 19.191458738866277 32.049949861381464 29.64959594172123 19.10899545803103 66-67 19.273218727828404 31.62324818574841 29.86639057780701 19.237142508616177 68-69 19.46489706836548 31.85234471774907 29.54917713678995 19.1335810770955 70-71 18.470170471303014 32.50433551583791 29.753648321830944 19.271845691028137 72-73 18.469770857065736 31.950286770791948 29.931400575901062 19.648541796241258 74-75 18.660660081066727 32.21299327998883 29.416335141239152 19.710011497705295 76-77 19.228309020875482 31.807277810876016 29.51344017837656 19.45097298987194 78-79 19.04651796007488 32.13168503673936 29.01536347450377 19.806433528681993 80-81 19.238427589038285 32.52018248291434 28.762907881992493 19.478482046054875 82-83 19.159106074176705 32.59585900065216 28.624105871260564 19.620929053910572 84-85 19.100342575405403 32.801518383668885 28.215693912803836 19.88244512812188 86-87 19.11627162922038 33.00826190444731 28.217555933489496 19.657910532842816 88-89 19.901303604081875 32.50152775320002 27.96050256591754 19.636666076800566 90-91 19.39530202102378 33.06113752663837 27.841008831951314 19.702551620386537 92-93 19.051896419512772 33.1550757977939 27.792791836253173 20.00023594644016 94-95 19.1711909396567 33.33871291216894 27.568052852002594 19.92204329617177 96-97 19.132112484571866 32.85145412001654 27.847512587304184 20.16892080810741 98-99 19.641141884767215 32.92308258583023 27.19398068198898 20.241794847413573 100 19.20028313572819 33.445832596000706 27.044511295935823 20.30937297233528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 90804.0 1 49119.5 2 5348.0 3 2788.5 4 2221.5 5 2010.5 6 1980.5 7 2206.0 8 2185.5 9 1977.0 10 1960.5 11 2887.0 12 19165.0 13 21523.5 14 7471.5 15 6726.5 16 7486.5 17 8509.5 18 9596.5 19 10678.5 20 11963.0 21 13633.5 22 15555.0 23 17778.5 24 20170.5 25 22769.0 26 25976.0 27 29448.5 28 32676.0 29 35692.0 30 38254.0 31 40581.0 32 43490.0 33 47113.0 34 50989.0 35 54300.5 36 56875.5 37 59095.5 38 60300.5 39 60780.5 40 61131.5 41 61618.5 42 61551.0 43 61288.0 44 61651.0 45 61738.0 46 61798.5 47 62135.0 48 61992.5 49 63465.0 50 72574.5 51 71802.5 52 57756.5 53 49740.5 54 46611.0 55 44422.5 56 39729.5 57 33537.5 58 29040.5 59 23754.0 60 18480.5 61 14938.5 62 11885.5 63 9278.5 64 6996.0 65 5190.5 66 4164.5 67 3306.0 68 2429.0 69 1780.0 70 1284.5 71 943.5 72 757.0 73 553.5 74 363.0 75 277.0 76 200.5 77 122.0 78 74.0 79 40.0 80 16.5 81 8.5 82 6.5 83 3.0 84 0.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1387836961009851 2 0.004247035922845514 3 0.003633575178434495 4 0.0 5 0.0027841679938653925 6 0.012410782752315226 7 0.0030673037220550936 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.718928803161682E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 7.314339644900607E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.1797322007904204E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.718928803161682E-5 64-65 0.0 66-67 2.359464401580841E-5 68-69 0.0 70-71 0.0 72-73 5.190821683477851E-4 74-75 3.067303722055094E-4 76-77 5.898661003952104E-4 78-79 3.7751430425293455E-4 80-81 0.0010617589807113785 82-83 0.001085353624727187 84-85 0.0013920839969326962 86-87 4.718928803161682E-5 88-89 0.0 90-91 4.718928803161682E-5 92-93 0.0 94-95 0.0 96-97 0.001203326844806229 98-99 6.842446764584439E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2119125.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.42212890925401 #Duplication Level Percentage of deduplicated Percentage of total 1 92.14346114589127 63.0465177666901 2 5.596343449151816 7.658274657966496 3 0.9481656912216032 1.9462654545628941 4 0.3321440944793809 0.9090402419566259 5 0.17275304341789532 0.5910065503102595 6 0.10303892765334617 0.42300856743411297 7 0.07656869444599486 0.3667295157257999 8 0.05921284063182959 0.3241174891835326 9 0.05187566810538844 0.31944992853245086 >10 0.4807637403097649 5.800392887657754 >50 0.020236797784331267 0.9466828635900939 >100 0.012266625202247017 1.8917121218685096 >500 0.0017913331377209629 0.8011627779350824 >1k 9.645639972343643E-4 1.49380677300423 >5k 2.0669228512164948E-4 1.0351239939922645 >10k+ 2.0669228512164948E-4 12.446708409589743 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 166733 7.868011561375568 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84202 3.9734324308381996 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 13096 0.6179909160620539 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 9549 0.45061051141390907 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6786 0.32022650858255175 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5623 0.2653453666017814 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4540 0.21423936766354038 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3863 0.1822922196661358 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3860 0.18215065180204093 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 2399 0.11320710198784877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010381643366955701 0.0 0.0 0.0 0.0 2 0.0010381643366955701 0.0 0.0 0.0 0.0 3 0.0010381643366955701 0.0 0.0 0.0 0.0 4 0.0010381643366955701 0.0 0.0 0.0 0.0 5 0.0010381643366955701 0.0 0.0 0.0 0.0 6 0.0010381643366955701 0.0 0.0 0.0 0.0 7 0.0010381643366955701 0.0 0.0 0.0 0.0 8 0.0010381643366955701 0.0 0.0 0.0 0.0 9 0.0010381643366955701 0.0 0.0 0.0 0.0 10-11 0.0010381643366955701 0.0 0.0 0.0 0.0 12-13 0.0010381643366955701 0.0 0.0 0.0 0.0 14-15 0.0010381643366955701 0.0 0.0 0.0 0.0 16-17 0.001085353624727187 0.0 0.0 0.0 0.0 18-19 0.0011797322007904205 0.0 0.0 0.0 0.0 20-21 0.0011797322007904205 0.0 0.0 0.0 0.0 22-23 0.0012269214888220375 0.0 0.0 0.0 0.0 24-25 0.0012977054208694627 0.0 0.0 0.0 2.3594644015808413E-5 26-27 0.001321300064885271 0.0 0.0 0.0 4.7189288031616826E-5 28-29 0.0013684893529168878 0.0 0.0 0.0 4.7189288031616826E-5 30-31 0.0015100572170117384 0.0 0.0 0.0 4.7189288031616826E-5 32-33 0.0016752197251223972 0.0 0.0 0.0 7.078393204742523E-5 34-35 0.00193476080929629 0.0 0.0 0.0 1.1797322007904206E-4 36-37 0.0022650858255176074 0.0 0.0 0.0 2.3594644015808411E-4 38-39 0.0029965197900076682 0.0 0.0 0.0 2.3594644015808411E-4 40-41 0.0045537662950510234 0.0 0.0 0.0 2.3594644015808411E-4 42-43 0.006866041408600247 0.0 0.0 0.0 2.3594644015808411E-4 44-45 0.009036748658054622 0.0 0.0 0.0 2.3594644015808411E-4 46-47 0.011490591635698696 0.0 0.0 0.0 2.3594644015808411E-4 48-49 0.014439922137674747 0.0 0.0 0.0 2.3594644015808411E-4 50-51 0.017790361587919544 0.0 0.0 0.0 2.3594644015808411E-4 52-53 0.022202560018875717 0.0 0.0 4.7189288031616826E-5 2.3594644015808411E-4 54-55 0.027110245974163866 0.0 0.0 2.3594644015808414E-4 2.3594644015808411E-4 56-57 0.0325370140977998 0.0 0.0 5.662714563794019E-4 2.3594644015808411E-4 58-59 0.038317701881672855 0.0 0.0 6.606500324426355E-4 2.3594644015808411E-4 60-61 0.04692974694744293 4.7189288031616826E-5 0.0 6.606500324426355E-4 2.3594644015808411E-4 62-63 0.05851471715920486 4.7189288031616826E-5 0.0 6.606500324426355E-4 2.3594644015808411E-4 64-65 0.07227039462042116 4.7189288031616826E-5 0.0 6.606500324426355E-4 2.3594644015808411E-4 66-67 0.08763050787471244 4.7189288031616826E-5 0.0 6.606500324426355E-4 2.3594644015808411E-4 68-69 0.10593995163097977 4.7189288031616826E-5 0.0 6.606500324426355E-4 2.3594644015808411E-4 70-71 0.13054916533946795 4.7189288031616826E-5 0.0 6.606500324426355E-4 2.3594644015808411E-4 72-73 0.1595705774789123 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 74-75 0.19265026838907567 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 76-77 0.23231286497964962 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 78-79 0.2811065888043414 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 80-81 0.3407774435203209 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 82-83 0.41590279006665487 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 84-85 0.503698460449478 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 86-87 0.6059576476139916 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 88 0.6918893411195659 4.7189288031616826E-5 0.0 7.078393204742523E-4 2.3594644015808411E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2840 0.0 87.01753 1 AACGAGT 42195 0.0 75.246574 1 ACGAGTG 46070 0.0 74.397835 2 GTGGAAT 50260 0.0 74.27584 6 CGAGTGG 47740 0.0 74.00742 3 TGGAATC 50500 0.0 73.86933 7 GGAATCA 51030 0.0 73.10788 8 GAATCAC 51085 0.0 73.04757 9 GAGTGGA 49665 0.0 72.954475 4 AGTGGAA 49870 0.0 72.61688 5 AACGTGT 1375 0.0 72.57698 1 TCGAGTG 1120 0.0 71.35466 2 CGTGTGG 1525 0.0 71.20542 3 ATCGAGT 1085 0.0 70.716965 1 ACGTGTG 1590 0.0 65.63678 2 AGCGAGT 580 0.0 57.623035 1 GCGAGTG 610 0.0 56.258022 2 TGAGTGG 2085 0.0 49.14975 3 ATGAGTG 1935 0.0 47.860435 2 GTGTGGA 2645 0.0 44.429863 4 >>END_MODULE