FastQCFastQC Report
Thu 26 May 2016
SRR2132780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589313
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27428910.593118715273125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA514971.9888286970327649No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215240.8312629643461413No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG148930.5751718699129846No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93520.3611768835980818No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76390.295020339371872No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64320.2484056581803745No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49650.19174970349277976No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43740.1689251164304972No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC30330.11713531736024188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT49150.089.543541
ATCGAGT16200.081.937041
CGTGTGG24600.079.49543
TCGAGTG17650.078.573822
AACGAGT709700.077.267571
GTGGAAT840300.077.005846
ACGAGTG773100.076.776922
TGGAATC843150.076.6879657
CGAGTGG800750.076.430113
GGAATCA850400.076.096088
GAATCAC852900.075.8399669
ACGTGTG24500.075.793432
AGTGGAA827250.075.7574845
GAGTGGA830300.075.530154
AACGTGT23650.075.2327351
GCGAGTG9100.066.125532
AGCGAGT8900.065.052091
TGAGTGG30450.059.4370463
ATGAGTG28500.058.2278022
GTGTGGA39100.054.1006854
Produced by FastQC (version 0.11.5)