##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132780_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2589313 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.05378762629315 31.0 25.0 33.0 16.0 34.0 2 28.291799793999413 31.0 26.0 34.0 16.0 34.0 3 29.32782402127514 31.0 28.0 34.0 16.0 34.0 4 34.81433028760911 35.0 35.0 37.0 32.0 37.0 5 33.839116012625745 35.0 35.0 37.0 28.0 37.0 6 35.29206511534141 37.0 35.0 37.0 32.0 37.0 7 35.63013818723345 37.0 35.0 37.0 33.0 37.0 8 35.92962882432521 37.0 35.0 37.0 35.0 37.0 9 37.9030936005033 39.0 38.0 39.0 35.0 39.0 10-11 37.89733029571937 39.0 38.0 39.0 35.0 39.0 12-13 37.89962028538072 39.0 38.0 39.0 35.0 39.0 14-15 39.34841886631705 41.0 39.0 41.0 36.0 41.0 16-17 39.33380186172935 41.0 39.0 41.0 36.0 41.0 18-19 39.240011539740465 41.0 39.0 41.0 36.0 41.0 20-21 39.16733898142094 40.5 39.0 41.0 35.5 41.0 22-23 39.03588577356233 40.0 39.0 41.0 35.0 41.0 24-25 38.95365295736745 40.0 39.0 41.0 35.0 41.0 26-27 38.75870645997606 40.0 39.0 41.0 35.0 41.0 28-29 38.534995576046626 40.0 39.0 41.0 35.0 41.0 30-31 38.34948594472742 40.0 38.0 41.0 34.5 41.0 32-33 38.18965455315754 40.0 38.0 41.0 34.0 41.0 34-35 38.118980208263736 40.0 38.0 41.0 34.0 41.0 36-37 37.913954975702055 40.0 38.0 41.0 34.0 41.0 38-39 37.2727729324342 40.0 36.0 41.0 33.0 41.0 40-41 36.97614289968034 40.0 35.0 41.0 33.0 41.0 42-43 36.807815625225686 40.0 35.0 41.0 33.0 41.0 44-45 36.652367828841086 39.0 35.0 41.0 33.0 41.0 46-47 36.51853966669924 39.0 35.0 41.0 32.0 41.0 48-49 36.34292880003306 39.0 35.0 41.0 31.0 41.0 50-51 36.00152376325303 39.0 35.0 41.0 30.5 41.0 52-53 35.350540471545926 39.0 35.0 41.0 27.5 41.0 54-55 34.08504784859922 39.0 34.5 41.0 13.5 41.0 56-57 33.02209041548859 39.0 34.0 41.0 2.0 41.0 58-59 32.576408105161484 38.5 33.0 41.0 2.0 41.0 60-61 32.322633262181895 38.0 33.0 40.0 2.0 41.0 62-63 32.057968658095795 37.5 33.0 40.0 2.0 41.0 64-65 31.776250881990705 37.0 33.0 40.0 2.0 41.0 66-67 31.46059553248294 36.5 33.0 40.0 2.0 41.0 68-69 31.12968787473743 36.0 32.0 39.5 2.0 41.0 70-71 30.78234149367033 35.5 32.0 39.0 2.0 41.0 72-73 30.43160830691384 35.0 32.0 39.0 2.0 40.0 74-75 30.072878018223363 35.0 31.0 37.5 2.0 40.0 76-77 29.716167763418326 35.0 31.0 37.0 2.0 39.0 78-79 29.349371242487873 35.0 31.0 37.0 2.0 39.0 80-81 29.00128972434001 35.0 31.0 36.0 2.0 39.0 82-83 28.67599880740567 35.0 30.0 36.0 2.0 37.0 84-85 28.37310688201851 35.0 30.0 35.0 2.0 37.0 86-87 28.1370730382924 35.0 30.0 35.0 2.0 36.5 88-89 27.884291122780443 35.0 29.0 35.0 2.0 36.0 90-91 27.658280207916157 35.0 29.0 35.0 2.0 36.0 92-93 27.457174354741973 34.5 29.0 35.0 2.0 36.0 94-95 27.24051476202375 34.0 29.0 35.0 2.0 35.5 96-97 27.024172628029135 34.0 28.0 35.0 2.0 35.0 98-99 26.830073266538268 34.0 27.0 35.0 2.0 35.0 100 26.713169477772677 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17601597908522848 1101 2 2.2844121572306015 1101 3 1.1412758122271072 1101 4 0.6279734768500091 1101 5 2.1859864628686054 1101 6 0.9475300301254777 1101 7 0.6017407533402945 1101 8 0.3950867442135504 1101 9 0.38474345088852857 1101 10-11 0.4621480345113369 1101 12-13 0.40477672415715205 1101 14-15 0.506596927815302 1101 16-17 0.44444380120169313 1101 18-19 0.41908591579645105 1101 20-21 0.38128067558425727 1101 22-23 0.3843935239019345 1101 24-25 0.3912874409164502 1101 26-27 0.2896373343560015 1101 28-29 0.3722071535297431 1101 30-31 0.3953282152770683 1101 32-33 0.39111780863999 1101 34-35 0.31720191451098856 1101 36-37 0.38732361506068 1101 38-39 0.4204669549328983 1101 40-41 0.4239336696949181 1101 42-43 0.43816867628817846 1101 44-45 0.5812768419176138 1101 46-47 0.5746711298555169 1101 48-49 0.48919235321955057 1101 50-51 0.5552467366145422 1101 52-53 0.6527325050523629 1101 54-55 0.6434694057307482 1101 56-57 0.5116499888821835 1101 58-59 0.5266631781428472 1101 60-61 0.618084509298015 1101 62-63 0.5654885717671121 1101 64-65 0.5538197497705717 1101 66-67 0.45278365022615574 1101 68-69 0.512267127901989 1101 70-71 0.5044967621423595 1101 72-73 0.5655998389096588 1101 74-75 0.5016434536833394 1101 76-77 0.4781488272107204 1101 78-79 0.4762556058088414 1101 80-81 0.4996093872353704 1101 82-83 0.46216927165178134 1101 84-85 0.4491840353919372 1101 86-87 0.535123627786799 1101 88-89 0.4916346715840838 1101 90-91 0.440605456714934 1101 92-93 0.4682572637054321 1101 94-95 0.4636071143839011 1101 96-97 0.4466020337683325 1101 98-99 0.5442249716856047 1101 100 0.5222694327798223 1102 1 1.2781973663334334 1102 2 2.896995223923277 1102 3 3.162266247177204 1102 4 1.3349319899377932 1102 5 2.5614230149333252 1102 6 1.1666829960818816 1102 7 0.7296907613981958 1102 8 0.5137679255441299 1102 9 0.39322902765619716 1102 10-11 0.4889798787009738 1102 12-13 0.44835688470592316 1102 14-15 0.5697278180975829 1102 16-17 0.513767800223917 1102 18-19 0.48560234695132465 1102 20-21 0.4580423167445318 1102 22-23 0.4288726338485489 1102 24-25 0.4667403941700883 1102 26-27 0.5225024747064353 1102 28-29 0.6091751246753034 1102 30-31 0.5869709824721525 1102 32-33 0.5682523962953994 1102 34-35 0.5783899162258948 1102 36-37 0.5809704898915911 1102 38-39 0.6658786844737961 1102 40-41 0.6654049495299006 1102 42-43 0.7337579387978863 1102 44-45 0.8167035905444067 1102 46-47 0.7951509846118086 1102 48-49 0.7720016182837952 1102 50-51 0.7878421251663354 1102 52-53 0.7919473517160966 1102 54-55 0.7383192693288834 1102 56-57 0.5455586898235651 1102 58-59 0.4817859782591256 1102 60-61 0.42813017344627724 1102 62-63 0.5054237311577197 1102 64-65 0.5478455759353231 1102 66-67 0.5292311294082666 1102 68-69 0.4787183678254614 1102 70-71 0.40558195472848624 1102 72-73 0.442130512190694 1102 74-75 0.478741313392252 1102 76-77 0.5058364337187058 1102 78-79 0.45208808117633126 1102 80-81 0.4525841324912996 1102 82-83 0.5083169521270321 1102 84-85 0.5043540588177997 1102 86-87 0.4924562201933185 1102 88-89 0.4814737681162029 1102 90-91 0.4347798604114459 1102 92-93 0.4628080327528714 1102 94-95 0.5292713360347889 1102 96-97 0.5236059652195202 1102 98-99 0.4910393406880118 1102 100 0.44522176094198684 1103 1 1.2085929164789064 1103 2 2.859105294759491 1103 3 3.0938929417917755 1103 4 1.332654716754135 1103 5 2.5700884647077515 1103 6 1.2037160992360612 1103 7 0.7102172891601484 1103 8 0.4775139756269908 1103 9 0.4424265218208703 1103 10-11 0.47565688293489927 1103 12-13 0.48414330319576493 1103 14-15 0.5653050672517566 1103 16-17 0.5419600270803002 1103 18-19 0.4993800979043215 1103 20-21 0.4796349244626228 1103 22-23 0.42039262288297863 1103 24-25 0.4140498815297917 1103 26-27 0.5087868281271426 1103 28-29 0.5796327904759266 1103 30-31 0.5999348051232687 1103 32-33 0.6098775566329877 1103 34-35 0.603566668319452 1103 36-37 0.5981875032183765 1103 38-39 0.608670854246057 1103 40-41 0.6507877742015253 1103 42-43 0.6319045942432737 1103 44-45 0.6403090060909165 1103 46-47 0.5972086926050508 1103 48-49 0.6068294803026504 1103 50-51 0.6979968389503526 1103 52-53 0.6237590056779752 1103 54-55 0.6972115044207357 1103 56-57 0.700795058731444 1103 58-59 0.6558810368718966 1103 60-61 0.5990684289507797 1103 62-63 0.6016328542394547 1103 64-65 0.6705976909248754 1103 66-67 0.6818156810440179 1103 68-69 0.6901277761022087 1103 70-71 0.6913337391715935 1103 72-73 0.6716084293194307 1103 74-75 0.6613795026746487 1103 76-77 0.6150844292873536 1103 78-79 0.5996878966118366 1103 80-81 0.5168142977306722 1103 82-83 0.5113029541057976 1103 84-85 0.5254630590445544 1103 86-87 0.5805335624194434 1103 88-89 0.5928075906883521 1103 90-91 0.5855970321297548 1103 92-93 0.6119087038862432 1103 94-95 0.6104865680208533 1103 96-97 0.6012013470457838 1103 98-99 0.6200136821616589 1103 100 0.741592462569109 1104 1 5.92108246523323 1104 2 4.952754551493868 1104 3 3.983791077882909 1104 4 1.7778478989278454 1104 5 2.8102530199081315 1104 6 1.2326935331760964 1104 7 0.6640338413440929 1104 8 0.49970967041501524 1104 9 0.44161674582005617 1104 10-11 0.45336910930106455 1104 12-13 0.3542428961500903 1104 14-15 0.4454632627005708 1104 16-17 0.3572864349912095 1104 18-19 0.31658686696712834 1104 20-21 0.3465035586425884 1104 22-23 0.326043442854008 1104 24-25 0.3066416609979896 1104 26-27 0.38061447724146547 1104 28-29 0.3747306033471176 1104 30-31 0.3924007650789534 1104 32-33 0.37456351220858153 1104 34-35 0.46004627881568183 1104 36-37 0.46684050891571616 1104 38-39 0.5611657756149313 1104 40-41 0.5111653082577519 1104 42-43 0.604405573288993 1104 44-45 0.6337456777454165 1104 46-47 0.5524411390973683 1104 48-49 0.6628677920070771 1104 50-51 0.7695265668781275 1104 52-53 0.7183682666413986 1104 54-55 0.7786507124239819 1104 56-57 0.7581542730351174 1104 58-59 0.8374149129327932 1104 60-61 0.8314214399344309 1104 62-63 0.7962210508857765 1104 64-65 0.7929085786685128 1104 66-67 0.7778456371460898 1104 68-69 0.8263930052528501 1104 70-71 0.8957700030252269 1104 72-73 0.8204080059832961 1104 74-75 0.8211732503753773 1104 76-77 0.8676392531508235 1104 78-79 0.868357678244184 1104 80-81 0.8935530074185358 1104 82-83 0.8346780079128564 1104 84-85 0.824436922029939 1104 86-87 0.8617193513145303 1104 88-89 0.8120309512048145 1104 90-91 0.787699040602952 1104 92-93 0.8450708165967882 1104 94-95 0.8571413872453206 1104 96-97 0.7835695696489573 1104 98-99 0.8220584312940069 1104 100 0.8100293769622162 1105 1 1.5220430570375925 1105 2 3.005665715274265 1105 3 3.184227478035762 1105 4 1.3821828646442214 1105 5 2.5506196078234424 1105 6 1.1184186691703104 1105 7 0.6902443710829615 1105 8 0.4840460652953311 1105 9 0.3885045729741492 1105 10-11 0.4016635263321504 1105 12-13 0.4274713669478345 1105 14-15 0.47076072717642603 1105 16-17 0.42912536484841013 1105 18-19 0.418708943876986 1105 20-21 0.3761275853930002 1105 22-23 0.3659944945155402 1105 24-25 0.39002256296881654 1105 26-27 0.4230791156950886 1105 28-29 0.5016237061998794 1105 30-31 0.4463635956612535 1105 32-33 0.48790920917805636 1105 34-35 0.46780078233960154 1105 36-37 0.5321527480840942 1105 38-39 0.5536304614267138 1105 40-41 0.5653421713986759 1105 42-43 0.5705326516411731 1105 44-45 0.6330083901743748 1105 46-47 0.6468669367060755 1105 48-49 0.6365318420829595 1105 50-51 0.6358372528042011 1105 52-53 0.6209938449502701 1105 54-55 0.6775198680459482 1105 56-57 0.62076715514835 1105 58-59 0.5586242318814598 1105 60-61 0.5334837245403179 1105 62-63 0.5259338952282491 1105 64-65 0.5782217671048322 1105 66-67 0.550143251799927 1105 68-69 0.5858851058596777 1105 70-71 0.5729246597159516 1105 72-73 0.5457037813515626 1105 74-75 0.5897548283260043 1105 76-77 0.5620426977095256 1105 78-79 0.5637982138165292 1105 80-81 0.5180501295685822 1105 82-83 0.5756833784330766 1105 84-85 0.4626904047751026 1105 86-87 0.48426581729627216 1105 88-89 0.4770584994446203 1105 90-91 0.3499629404587594 1105 92-93 0.4684727287507755 1105 94-95 0.4929552408532558 1105 96-97 0.49591007395439135 1105 98-99 0.5518584409550691 1105 100 0.6619960401875211 1106 1 -0.7693718807406249 1106 2 1.8349860422490742 1106 3 2.7107244203584564 1106 4 1.1568050477700496 1106 5 2.440107252830451 1106 6 1.0670575104497075 1106 7 0.5791695909763703 1106 8 0.42206200303682806 1106 9 0.31045753307096646 1106 10-11 0.386222761230826 1106 12-13 0.3204357568139429 1106 14-15 0.3376735910715567 1106 16-17 0.3490144681607674 1106 18-19 0.2675634643410518 1106 20-21 0.33613431862159615 1106 22-23 0.3145987596132187 1106 24-25 0.2869453568682161 1106 26-27 0.23693375732129596 1106 28-29 0.3472901545630549 1106 30-31 0.32537711788477 1106 32-33 0.30437259329261224 1106 34-35 0.3738183553995711 1106 36-37 0.36291112147235793 1106 38-39 0.41868176697783355 1106 40-41 0.43370080645483 1106 42-43 0.3841723759756164 1106 44-45 0.4513700251838628 1106 46-47 0.4417500215935348 1106 48-49 0.4405763982152564 1106 50-51 0.6362993884725654 1106 52-53 0.6850541712076321 1106 54-55 0.5940280186598983 1106 56-57 0.48202947202024404 1106 58-59 0.5516373564392012 1106 60-61 0.5777957072004867 1106 62-63 0.5084223686879739 1106 64-65 0.5465847724633726 1106 66-67 0.5418239632216633 1106 68-69 0.5288540617923196 1106 70-71 0.5047686299737677 1106 72-73 0.48850586157317366 1106 74-75 0.4996020508083667 1106 76-77 0.49800565151331355 1106 78-79 0.4767277693038672 1106 80-81 0.44920045982270906 1106 82-83 0.4197076830285482 1106 84-85 0.38000833115233235 1106 86-87 0.33589037403596933 1106 88-89 0.34698830053660146 1106 90-91 0.3974033929723575 1106 92-93 0.40329678492683385 1106 94-95 0.422777628801299 1106 96-97 0.5122944751705809 1106 98-99 0.45310640566927063 1106 100 0.48516794428594423 1107 1 -1.0912324285102208 1107 2 1.7049499609591976 1107 3 -0.6778607036767426 1107 4 -0.08162355779990094 1107 5 1.8132066479317714 1107 6 0.7246026098654568 1107 7 0.3380713521633041 1107 8 0.28629198183088533 1107 9 0.2551185741082165 1107 10-11 0.284727686438913 1107 12-13 0.24967372682512945 1107 14-15 0.3535432575711397 1107 16-17 0.2631610531785 1107 18-19 0.24572723838335264 1107 20-21 0.19934842656848417 1107 22-23 0.1868838764875207 1107 24-25 0.1136388282327232 1107 26-27 0.13394101575391915 1107 28-29 0.1818158644041148 1107 30-31 0.20850040536342362 1107 32-33 0.13772002145182682 1107 34-35 0.16311925130332128 1107 36-37 0.3231396322811477 1107 38-39 0.25267106801651806 1107 40-41 0.22713577885899383 1107 42-43 0.21469789185723442 1107 44-45 0.3155192516085634 1107 46-47 0.33407465852913276 1107 48-49 0.23508970717223576 1107 50-51 0.26062794391619093 1107 52-53 0.3055144932110707 1107 54-55 0.30777915594759975 1107 56-57 0.2591381330235052 1107 58-59 0.25315205232703875 1107 60-61 0.25903024590868995 1107 62-63 0.2565907369113134 1107 64-65 0.22407600914266723 1107 66-67 0.27525440624436825 1107 68-69 0.26491048070782597 1107 70-71 0.3278304962282945 1107 72-73 0.27909517100285086 1107 74-75 0.286194159140976 1107 76-77 0.2662809753025819 1107 78-79 0.2610870924710582 1107 80-81 0.25136187757621187 1107 82-83 0.2674424870855745 1107 84-85 0.299975594180232 1107 86-87 0.31039047266314057 1107 88-89 0.2527594013695982 1107 90-91 0.2111297526544469 1107 92-93 0.14327121764487316 1107 94-95 0.04856579499049474 1107 96-97 0.14298755839653765 1107 98-99 0.09410572626205393 1107 100 0.14316367542080854 1108 1 -1.3442792315745038 1108 2 0.3725100197105178 1108 3 1.9669844232362124 1108 4 0.8527513241444424 1108 5 0.711867845615771 1108 6 0.4345271247961122 1108 7 0.24375228124252146 1108 8 0.1742540943295623 1108 9 0.126087442917715 1108 10-11 0.2692356270613061 1108 12-13 0.2499843696532409 1108 14-15 0.3149180364230233 1108 16-17 0.21863455705208423 1108 18-19 0.19196972890590303 1108 20-21 0.1533037631847023 1108 22-23 0.11929772601440192 1108 24-25 0.12367830945692049 1108 26-27 0.13452298745515634 1108 28-29 0.1307956530486578 1108 30-31 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2215 68-69 -0.8424551622864165 2215 70-71 -0.8730612447703905 2215 72-73 -0.8573824739409339 2215 74-75 -0.9407768593981665 2215 76-77 -0.8978337456957703 2215 78-79 -0.9356884999070445 2215 80-81 -0.8944305767958163 2215 82-83 -0.9908192381435974 2215 84-85 -1.0001661100942698 2215 86-87 -0.9680504617168246 2215 88-89 -1.0545564086102353 2215 90-91 -1.0063978610129816 2215 92-93 -0.9765206972951113 2215 94-95 -0.9469499025306476 2215 96-97 -1.0077834548893456 2215 98-99 -1.0048630902853297 2215 100 -0.9777183519097683 2216 1 2.910427526826094 2216 2 -1.7935727471456744 2216 3 -2.302453365254493 2216 4 -0.8718785525986732 2216 5 -2.5121010090420555 2216 6 -1.0925964518522804 2216 7 -0.6274664081662777 2216 8 -0.3828488437952515 2216 9 -0.3610178322614743 2216 10-11 -0.2164971418658581 2216 12-13 -0.23517582874641363 2216 14-15 -0.3090784970242595 2216 16-17 -0.36347624011316526 2216 18-19 -0.3934252610876854 2216 20-21 -0.3893834159283003 2216 22-23 -0.39665078624026506 2216 24-25 -0.39236816471691327 2216 26-27 -0.38774897967370237 2216 28-29 -0.33621573563508633 2216 30-31 -0.3252514238999993 2216 32-33 -0.3866507729248738 2216 34-35 -0.4243493530890987 2216 36-37 -0.43645343206055287 2216 38-39 -0.3786842158468886 2216 40-41 -0.3754936019613666 2216 42-43 -0.45517874038836936 2216 44-45 -0.4206970460926698 2216 46-47 -0.3803460102230787 2216 48-49 -0.4095855746468544 2216 50-51 -0.3209765540842895 2216 52-53 -0.3738282223139109 2216 54-55 -0.25177688145244304 2216 56-57 -0.06975341326617013 2216 58-59 -0.14544938929447682 2216 60-61 -0.13671501567335298 2216 62-63 -0.0268968245180794 2216 64-65 -0.13865982860424708 2216 66-67 -0.10263889538103044 2216 68-69 -0.04347422869009776 2216 70-71 -0.10999694665599336 2216 72-73 -0.08292058158945537 2216 74-75 -0.15408446517721686 2216 76-77 -0.13599537187447552 2216 78-79 -0.190299982402653 2216 80-81 -0.15982956548810634 2216 82-83 -0.3257253323482985 2216 84-85 -0.2327308102502954 2216 86-87 -0.26484252049765544 2216 88-89 -0.30541619677167375 2216 90-91 -0.2699176008183457 2216 92-93 -0.2820949137138058 2216 94-95 -0.372522993743722 2216 96-97 -0.43780205243884396 2216 98-99 -0.43348673277695937 2216 100 -0.48226432700040434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1359.0 3 344.0 4 377.0 5 789.0 6 1922.0 7 3275.0 8 4217.0 9 5802.0 10 4927.0 11 7017.0 12 6176.0 13 8866.0 14 9103.0 15 9894.0 16 11871.0 17 14175.0 18 21511.0 19 39565.0 20 82283.0 21 99619.0 22 42893.0 23 24825.0 24 22283.0 25 22433.0 26 22780.0 27 24305.0 28 27895.0 29 32144.0 30 35875.0 31 43082.0 32 53853.0 33 73591.0 34 105406.0 35 196622.0 36 281749.0 37 476090.0 38 599376.0 39 170481.0 40 538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.462713379249747 47.89487464386032 22.608116213980438 17.034295762909498 2 13.379677603620923 47.990420107115284 22.104509818386024 16.52539247087777 3 16.21671307686069 23.2697751841281 17.490373585969575 43.02313815304164 4 40.203328064239436 24.481551670269294 19.17751928793468 16.137600977556595 5 15.584171317629167 47.034371742540024 19.5121056337185 17.869351306112314 6 43.1116307436552 21.79443949403034 20.850410330748172 14.243519431566288 7 16.639631706601165 21.393768055491186 46.15582178554325 15.8107784523644 8 42.52390498947018 21.828840314013796 20.20922151937599 15.438033177140037 9 41.879023509324675 23.19012803782316 20.355824112419008 14.575024340433156 10-11 14.645892559145999 49.618315746300276 20.650767211225528 15.085024483328201 12-13 14.517480119243986 22.76347046494572 33.73948997282291 28.979559442987384 14-15 14.8399015491754 35.82390386948198 20.56854462940556 28.76764995193706 16-17 28.56060661650407 22.292341636565375 33.86523761322018 15.28181413371037 18-19 15.159580939036726 22.58448476487779 33.98880320764619 28.26713108843929 20-21 28.563503137704867 36.17727945597925 20.57984106208867 14.679376344227213 22-23 28.089902611233175 22.850501271959008 34.10099512882375 14.958600987984072 24-25 14.770114698377522 36.04235949844611 20.656193360941685 28.53133244223468 26-27 15.283497067271718 28.365251393951098 41.36939824760433 14.98185329117285 28-29 27.216678709758146 22.59775083197744 35.33427592569921 14.851294532565202 30-31 23.3412878242221 24.320678882777013 36.24552149547004 16.09251179753085 32-33 17.550060575913378 26.758545606498714 37.660607273048875 18.030786544539033 34-35 17.76463486646844 26.289425032817586 38.19105685562155 17.754883245092422 36-37 17.82876384585409 26.267372851408847 38.37454181862139 17.52932148411567 38-39 18.325691023062873 26.327543251820078 37.862127908059016 17.484637817058037 40-41 17.31662027727046 27.723260957636253 37.66522239682881 17.294896368264478 42-43 17.34297484194995 26.53159234160864 38.334613284990624 17.790819531450797 44-45 17.304667299781833 27.344144952734567 37.56156555812294 17.789622189360653 46-47 17.9669665274148 26.722049439368668 37.844072925907376 17.46691110730916 48-49 17.607257214558455 26.928436229996144 37.65655214336776 17.807754412077646 50-51 17.962621745613603 27.58668032794799 36.80495560019202 17.645742326246385 52-53 18.212932139990272 27.451769458911517 36.39294043177937 17.942357969318838 54-55 19.13887969511604 28.671833030614685 33.41946300041749 18.76982427385179 56-57 20.153685552886035 29.745225857206137 30.948614555289378 19.15247403461845 58-59 21.036371423617 30.509849523792603 29.401524651519534 19.052254401070865 60-61 20.371561877610006 30.834935753228756 29.393008879189193 19.40049348997205 62-63 19.43039684487556 30.96774953496325 29.35944555145258 20.242408068708613 64-65 19.634435852289776 30.7959871981487 30.327793511251826 19.2417834383097 66-67 19.55171120679501 30.375489560358286 30.796836844367597 19.27596238847911 68-69 19.709204719553025 30.52089492463831 30.521860431705246 19.248039924103423 70-71 18.58865652781259 31.29154335532244 30.6422012325277 19.477598884337276 72-73 18.593589544050694 30.725222753167746 30.847340652940698 19.833847049840863 74-75 18.757905119640306 31.206139258325504 30.102212834882593 19.9337427871516 76-77 19.461058765326058 30.81492326687648 30.015903918396447 19.708114049401015 78-79 19.281745210295895 31.014357526192367 29.544367907360435 20.159529356151307 80-81 19.558310157676857 31.4890068022692 29.1440526199724 19.808630420081546 82-83 19.385744655418886 31.45575326591566 29.243614493055315 19.91488758561014 84-85 19.078197564955442 31.76813973023337 28.851647661990327 20.302015042820866 86-87 19.131900507142536 31.94754667408691 28.884647521165373 20.035905297605176 88-89 20.187150027825915 31.463886366769874 28.3801533456944 19.968810259709816 90-91 19.628905212833605 32.146306074027784 28.20506532334947 20.01972338978914 92-93 19.055807467077173 32.358042461455994 28.05865880254724 20.527491268919594 94-95 19.336835421919027 32.50890584062485 27.756176157991003 20.398082579465125 96-97 19.28753746708053 31.85757160407574 28.121464982641847 20.73342594620189 98-99 19.926142043711135 31.970851519593463 27.330398299614046 20.772608137081356 100 19.218842990399384 32.64630425135934 27.216138025800667 20.918714732440613 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 54874.0 1 29636.0 2 3189.0 3 1711.5 4 1414.0 5 1323.0 6 1340.0 7 1503.0 8 1506.0 9 1389.0 10 1377.5 11 2157.0 12 16255.0 13 19122.0 14 8142.5 15 8237.0 16 9673.5 17 11285.0 18 13124.5 19 15154.5 20 17306.5 21 19828.0 22 22365.5 23 25398.0 24 28929.0 25 32807.0 26 36830.5 27 40582.0 28 43910.5 29 47069.0 30 50861.5 31 55023.5 32 58991.5 33 63194.0 34 67820.0 35 71659.5 36 74578.5 37 77073.5 38 78369.0 39 78529.0 40 78412.5 41 78885.5 42 78636.5 43 77744.0 44 77832.0 45 78034.0 46 77884.0 47 77721.5 48 77755.0 49 80300.5 50 93309.0 51 92043.5 52 71842.5 53 60185.5 54 54439.0 55 50347.5 56 44048.5 57 36336.0 58 30540.0 59 24459.0 60 18640.5 61 14502.5 62 11540.5 63 8925.0 64 6785.5 65 5179.5 66 3986.0 67 3189.0 68 2604.0 69 1963.0 70 1411.0 71 1035.0 72 806.0 73 604.0 74 436.5 75 330.5 76 214.5 77 123.5 78 77.5 79 44.5 80 18.5 81 11.0 82 9.0 83 7.0 84 3.0 85 1.5 86 2.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13903301763826928 2 0.005406839574821584 3 0.003205483462215653 4 0.0 5 0.00247169809134701 6 0.011740565933898297 7 0.0028192806354426832 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.9310141338648514E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.689563602391833E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.737912720478366E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 5.793042401594555E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.634433921275644E-4 74-75 3.862028267729703E-4 76-77 5.406839574821584E-4 78-79 3.475825440956732E-4 80-81 0.0011586084803189107 82-83 0.0012165389043348562 84-85 0.0014675707417372871 86-87 5.793042401594555E-5 88-89 0.0 90-91 5.793042401594555E-5 92-93 0.0 94-95 3.862028267729703E-5 96-97 0.001544811307091881 98-99 7.144752295299951E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2589313.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.59077638365814 #Duplication Level Percentage of deduplicated Percentage of total 1 92.07447329412068 60.392361884777735 2 5.100107059742856 6.690399633766199 3 0.9578663995862716 1.8848160246204861 4 0.43402374458970416 1.1387181750632498 5 0.2555294538759179 0.838018763430681 6 0.1800428472666712 0.7085490080727208 7 0.13047193781481492 0.5990428988287855 8 0.11166097368598268 0.5859143964655061 9 0.08949100057625235 0.5282805786432112 >10 0.6319536473670057 6.767887338757907 >50 0.01904606888797929 0.8508011842622105 >100 0.011443988991569625 1.7472813748979903 >500 0.002710921829338276 1.2722023953075545 >1k 7.66130082203657E-4 1.0894817851155134 >5k 1.767992497393055E-4 0.9054082615845545 >10k+ 2.3573233298574062E-4 14.000836296405685 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 274289 10.593118715273125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51497 1.9888286970327649 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 21524 0.8312629643461413 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 14893 0.5751718699129846 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9352 0.3611768835980818 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 7639 0.295020339371872 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6432 0.2484056581803745 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4965 0.19174970349277976 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4374 0.1689251164304972 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 3033 0.11713531736024188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001235849045673505 0.0 0.0 0.0 0.0 2 0.001235849045673505 0.0 0.0 0.0 0.0 3 0.001235849045673505 0.0 0.0 0.0 0.0 4 0.001235849045673505 0.0 0.0 0.0 0.0 5 0.001235849045673505 0.0 0.0 0.0 0.0 6 0.001235849045673505 0.0 0.0 0.0 0.0 7 0.001235849045673505 0.0 0.0 0.0 3.862028267729703E-5 8 0.001235849045673505 0.0 0.0 0.0 7.724056535459406E-5 9 0.001235849045673505 0.0 0.0 0.0 7.724056535459406E-5 10-11 0.001235849045673505 0.0 0.0 0.0 7.724056535459406E-5 12-13 0.001235849045673505 0.0 0.0 0.0 7.724056535459406E-5 14-15 0.001274469328350802 0.0 0.0 0.0 7.724056535459406E-5 16-17 0.001274469328350802 0.0 0.0 0.0 7.724056535459406E-5 18-19 0.0012937794696894504 0.0 0.0 0.0 7.724056535459406E-5 20-21 0.001313089611028099 0.0 0.0 0.0 7.724056535459406E-5 22-23 0.001313089611028099 0.0 0.0 0.0 1.1586084803189108E-4 24-25 0.0013903301763826931 0.0 0.0 0.0 1.5448113070918812E-4 26-27 0.0015061910244145841 0.0 0.0 0.0 1.9310141338648514E-4 28-29 0.0015255011657532326 0.0 0.0 0.0 1.9310141338648514E-4 30-31 0.001622051872446475 0.0 0.0 0.0 1.9310141338648514E-4 32-33 0.0017186025791397178 0.0 0.0 0.0 2.3172169606378216E-4 34-35 0.0018923938511875545 0.0 0.0 0.0 2.3172169606378216E-4 36-37 0.0023558372433151184 0.0 0.0 0.0 2.3172169606378216E-4 38-39 0.003147553038199708 0.0 0.0 0.0 2.3172169606378216E-4 40-41 0.004286851377179971 0.0 0.0 0.0 2.3172169606378216E-4 42-43 0.0057737322602559055 0.0 0.0 0.0 2.3172169606378216E-4 44-45 0.007453714556718326 0.0 0.0 0.0 2.3172169606378216E-4 46-47 0.00961645038664696 0.0 0.0 0.0 2.3172169606378216E-4 48-49 0.012435731022089643 0.0 0.0 0.0 2.3172169606378216E-4 50-51 0.015757075332337188 0.0 0.0 0.0 2.3172169606378216E-4 52-53 0.019503242752035 0.0 0.0 0.0 2.703419787410792E-4 54-55 0.024214917238665236 0.0 0.0 0.0 2.703419787410792E-4 56-57 0.029332104693407093 0.0 0.0 3.862028267729703E-5 2.703419787410792E-4 58-59 0.03607134402059542 0.0 0.0 3.862028267729703E-5 2.703419787410792E-4 60-61 0.04576503497259698 0.0 0.0 3.862028267729703E-5 2.703419787410792E-4 62-63 0.05640492285019231 0.0 0.0 3.862028267729703E-5 2.896521200797277E-4 64-65 0.06913030599236168 0.0 0.0 3.862028267729703E-5 3.0896226141837623E-4 66-67 0.08417290609516888 0.0 0.0 3.862028267729703E-5 3.475825440956732E-4 68-69 0.10325132573775361 0.0 0.0 7.724056535459406E-5 3.475825440956732E-4 70-71 0.12725383142169372 0.0 0.0 7.724056535459406E-5 3.475825440956732E-4 72-73 0.15548525805879782 0.0 0.0 1.1586084803189108E-4 3.475825440956732E-4 74-75 0.18680630731008574 0.0 0.0 1.1586084803189108E-4 3.862028267729703E-4 76-77 0.22531072913935085 0.0 0.0 1.1586084803189108E-4 3.862028267729703E-4 78-79 0.26953095280485595 0.0 0.0 1.1586084803189108E-4 4.055129681116188E-4 80-81 0.3243910643479564 0.0 0.0 1.1586084803189108E-4 4.248231094502673E-4 82-83 0.39244000242535376 0.0 0.0 1.1586084803189108E-4 4.248231094502673E-4 84-85 0.4756474014535902 0.0 0.0 1.1586084803189108E-4 4.8275353346621287E-4 86-87 0.5738394701606179 0.0 0.0 1.1586084803189108E-4 5.406839574821584E-4 88 0.6605226946298111 0.0 0.0 1.1586084803189108E-4 5.406839574821584E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 4915 0.0 89.54354 1 ATCGAGT 1620 0.0 81.93704 1 CGTGTGG 2460 0.0 79.4954 3 TCGAGTG 1765 0.0 78.57382 2 AACGAGT 70970 0.0 77.26757 1 GTGGAAT 84030 0.0 77.00584 6 ACGAGTG 77310 0.0 76.77692 2 TGGAATC 84315 0.0 76.687965 7 CGAGTGG 80075 0.0 76.43011 3 GGAATCA 85040 0.0 76.09608 8 GAATCAC 85290 0.0 75.839966 9 ACGTGTG 2450 0.0 75.79343 2 AGTGGAA 82725 0.0 75.757484 5 GAGTGGA 83030 0.0 75.53015 4 AACGTGT 2365 0.0 75.232735 1 GCGAGTG 910 0.0 66.12553 2 AGCGAGT 890 0.0 65.05209 1 TGAGTGG 3045 0.0 59.437046 3 ATGAGTG 2850 0.0 58.227802 2 GTGTGGA 3910 0.0 54.100685 4 >>END_MODULE