FastQCFastQC Report
Thu 26 May 2016
SRR2132779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123612
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT936508.334727646198154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255092.270267672470568No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG137491.223643036920218No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74480.6628622691818884No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30410.2706450269310047No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25530.2272136645034051No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21980.19561912831119638No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21300.1895672171532522No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20800.1851172824782932No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC15500.13794797492372812No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA11550.10279349099155223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT17950.087.607831
AACGTGT9150.076.670111
ATCGAGT7150.075.727341
CGTGTGG10450.072.4161453
TCGAGTG8100.071.381322
ACGTGTG10250.069.708252
GTGGAAT364300.064.021146
TGGAATC367300.063.4797867
GGAATCA370400.062.897758
GAATCAC371250.062.690449
AGTGGAA360900.062.4623225
ACGAGTG344700.061.6398622
CGAGTGG357950.061.5064053
GAGTGGA368850.061.0523224
AACGAGT321150.060.973611
AGCGAGT3800.059.472821
TGAGTGG11100.054.6251373
GCGAGTG4350.054.0312732
GTGTGGA15850.050.413344
AATGAGT10800.050.13431