##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132779_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123612
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.046637985354376	31.0	25.0	33.0	16.0	34.0
2	28.313628725930304	31.0	26.0	34.0	16.0	34.0
3	29.382333047350865	31.0	28.0	34.0	16.0	34.0
4	34.86298562137108	35.0	35.0	37.0	32.0	37.0
5	33.917247234810596	35.0	35.0	37.0	28.0	37.0
6	35.35034068699871	37.0	35.0	37.0	32.0	37.0
7	35.71645016251161	37.0	35.0	37.0	35.0	37.0
8	35.99828944511095	37.0	35.0	37.0	35.0	37.0
9	37.973632357077	39.0	38.0	39.0	35.0	39.0
10-11	37.96328536897079	39.0	38.5	39.0	35.0	39.0
12-13	37.95458797164858	39.0	38.5	39.0	35.0	39.0
14-15	39.40830242112045	41.0	39.0	41.0	36.5	41.0
16-17	39.377914262218624	41.0	39.0	41.0	36.0	41.0
18-19	39.25566788179549	41.0	39.0	41.0	36.0	41.0
20-21	39.171057268879295	40.5	39.0	41.0	35.0	41.0
22-23	39.02482796552547	40.0	39.0	41.0	35.0	41.0
24-25	38.857322189510256	40.0	39.0	41.0	35.0	41.0
26-27	38.715904155527	40.0	39.0	41.0	35.0	41.0
28-29	38.582330466388754	40.0	39.0	41.0	35.0	41.0
30-31	38.436868331772885	40.0	38.0	41.0	35.0	41.0
32-33	38.285455744509676	40.0	38.0	41.0	34.0	41.0
34-35	38.18623866601638	40.0	38.0	41.0	34.0	41.0
36-37	37.975166694552925	40.0	38.0	41.0	34.0	41.0
38-39	37.431038472355226	40.0	36.0	41.0	33.5	41.0
40-41	37.181520400280526	40.0	35.0	41.0	33.0	41.0
42-43	37.02138238110665	40.0	35.0	41.0	33.0	41.0
44-45	36.8571286173519	40.0	35.0	41.0	33.0	41.0
46-47	36.70688013299965	39.5	35.0	41.0	33.0	41.0
48-49	36.52707340256245	39.0	35.0	41.0	32.0	41.0
50-51	36.20595766154153	39.0	35.0	41.0	31.0	41.0
52-53	35.65962004677771	39.0	35.0	41.0	29.0	41.0
54-55	34.62594872607271	39.0	35.0	41.0	22.0	41.0
56-57	33.84186578641025	39.0	35.0	41.0	9.5	41.0
58-59	33.471117698992174	38.0	34.0	40.0	3.5	41.0
60-61	33.20695622688259	38.0	34.0	40.0	2.0	41.0
62-63	32.94065433619434	37.0	34.0	40.0	2.0	41.0
64-65	32.63579954646266	36.5	34.0	40.0	2.0	41.0
66-67	32.28876071099276	36.0	33.5	39.5	2.0	41.0
68-69	31.967709939018096	35.5	33.0	39.0	2.0	41.0
70-71	31.63144484038974	35.0	33.0	39.0	2.0	40.5
72-73	31.299751604646445	35.0	33.0	38.0	2.0	40.0
74-75	30.950128692110802	35.0	33.0	37.0	2.0	40.0
76-77	30.610157687885142	35.0	33.0	37.0	2.0	39.0
78-79	30.25867203269456	35.0	32.0	36.0	2.0	39.0
80-81	29.934423092669	35.0	32.0	36.0	2.0	38.5
82-83	29.60665202934821	35.0	32.0	35.5	2.0	37.0
84-85	29.291873885291363	35.0	31.0	35.0	2.0	37.0
86-87	29.09642251951741	35.0	31.0	35.0	2.0	36.5
88-89	28.878403755032878	35.0	31.0	35.0	2.0	36.0
90-91	28.66288140390099	35.0	31.0	35.0	2.0	36.0
92-93	28.49208490119365	35.0	31.0	35.0	2.0	35.5
94-95	28.27928190514163	35.0	30.5	35.0	2.0	35.0
96-97	28.05372940125239	34.5	30.0	35.0	2.0	35.0
98-99	27.875651470436413	34.0	30.0	35.0	2.0	35.0
100	27.778055058151747	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.35946314700956705
1101	2	2.297260528549831
1101	3	1.0634558758858503
1101	4	0.6865535499788677
1101	5	2.2149757045969096
1101	6	0.9692916616457694
1101	7	0.5310035133450057
1101	8	0.36972451424470876
1101	9	0.31124297685363445
1101	10-11	0.43724088750327894
1101	12-13	0.34813521284080196
1101	14-15	0.4318647304695702
1101	16-17	0.36988749360802586
1101	18-19	0.3649657652096465
1101	20-21	0.3776920123997627
1101	22-23	0.35524897884703677
1101	24-25	0.3028617921131058
1101	26-27	0.32180688391513
1101	28-29	0.3679872798863002
1101	30-31	0.19685890895797797
1101	32-33	0.27290820632777724
1101	34-35	0.3504725640389026
1101	36-37	0.294091735860043
1101	38-39	0.23004854980298717
1101	40-41	0.2454058376778505
1101	42-43	0.3528723478522977
1101	44-45	0.2584427656129691
1101	46-47	0.23426761792208595
1101	48-49	0.10373965413119635
1101	50-51	0.17377225131673413
1101	52-53	0.1804922908347848
1101	54-55	0.10906564492606208
1101	56-57	-0.032919774872695484
1101	58-59	0.008854696642714543
1101	60-61	0.07493651452758598
1101	62-63	0.07183385531615727
1101	64-65	0.07830645057100583
1101	66-67	-0.00767598083250931
1101	68-69	0.08847362999035013
1101	70-71	0.006769971686821918
1101	72-73	0.06495903842587225
1101	74-75	0.06131520199155105
1101	76-77	-0.02744567211130544
1101	78-79	0.0991025800737475
1101	80-81	0.04786154541020338
1101	82-83	0.08902376597809081
1101	84-85	0.05255406930406181
1101	86-87	0.13366985327114023
1101	88-89	0.09274783331841974
1101	90-91	0.04355642650811831
1101	92-93	0.019119232430682587
1101	94-95	0.05659154697413271
1101	96-97	0.1174503179538462
1101	98-99	0.2741677490738681
1101	100	0.2691175193873363
1102	1	1.7180794560796713
1102	2	3.0914987413171033
1102	3	3.242968218235596
1102	4	1.3877990956484751
1102	5	2.6165619403141775
1102	6	1.1297436965589114
1102	7	0.6747246487483167
1102	8	0.45318345582556674
1102	9	0.34706037698911985
1102	10-11	0.4361425594621693
1102	12-13	0.3583569560451565
1102	14-15	0.4944435332741435
1102	16-17	0.33787420770817533
1102	18-19	0.2062268541557728
1102	20-21	0.17660570941361442
1102	22-23	0.14729693500439822
1102	24-25	0.21741499583778534
1102	26-27	0.18824677114155008
1102	28-29	0.18252000529952284
1102	30-31	0.31768972714985466
1102	32-33	0.26546398293797324
1102	34-35	0.2906258894428433
1102	36-37	0.25101316700826715
1102	38-39	0.3194247709115956
1102	40-41	0.39463364460547723
1102	42-43	0.49560290446946453
1102	44-45	0.42615314156856954
1102	46-47	0.3655712469884307
1102	48-49	0.34626373102964436
1102	50-51	0.3935799873560626
1102	52-53	0.46297020819051227
1102	54-55	0.5615333409850578
1102	56-57	0.579439332558664
1102	58-59	0.5338940459562806
1102	60-61	0.5580136308479666
1102	62-63	0.5274923580420321
1102	64-65	0.47166653667515845
1102	66-67	0.5420931619239369
1102	68-69	0.5091051418255041
1102	70-71	0.5732917423940265
1102	72-73	0.6038455064291064
1102	74-75	0.6727635008034483
1102	76-77	0.6575696211393094
1102	78-79	0.7663801731101714
1102	80-81	0.6922032808042253
1102	82-83	0.5834608386272819
1102	84-85	0.6228226566458481
1102	86-87	0.6028666629407127
1102	88-89	0.5339755259484917
1102	90-91	0.6092578741580539
1102	92-93	0.629800338688117
1102	94-95	0.5308167048178589
1102	96-97	0.4764302063470467
1102	98-99	0.40741518065308924
1102	100	0.4060453916788731
1103	1	1.223474162062839
1103	2	2.9327743589824706
1103	3	3.2151273296453766
1103	4	1.3680412457283069
1103	5	2.611709580151455
1103	6	1.1972899932728183
1103	7	0.716583936505053
1103	8	0.4649758129213879
1103	9	0.36476836266487567
1103	10-11	0.43632197896045
1103	12-13	0.442072430828901
1103	14-15	0.5653250876530507
1103	16-17	0.4345073225997922
1103	18-19	0.38610540139059424
1103	20-21	0.38030180745531084
1103	22-23	0.3899785081004339
1103	24-25	0.3675621959352
1103	26-27	0.35456469967367354
1103	28-29	0.3946024497569951
1103	30-31	0.4386527179671873
1103	32-33	0.5478123459781798
1103	34-35	0.574919678512039
1103	36-37	0.4632313388631175
1103	38-39	0.5281126210121769
1103	40-41	0.5517501576262873
1103	42-43	0.5372180802301045
1103	44-45	0.6256566301714201
1103	46-47	0.5573780196587066
1103	48-49	0.5365965295340729
1103	50-51	0.6092110760617402
1103	52-53	0.5460579261436251
1103	54-55	0.42655772963390604
1103	56-57	0.29830913348054366
1103	58-59	0.2764638518393241
1103	60-61	0.17596609496970217
1103	62-63	0.21147655334065263
1103	64-65	0.380928331851095
1103	66-67	0.3848762980132747
1103	68-69	0.3920227142811008
1103	70-71	0.37218334426484034
1103	72-73	0.37897100141832496
1103	74-75	0.34648315311580546
1103	76-77	0.3854825594133615
1103	78-79	0.3365098005264855
1103	80-81	0.26469552334217994
1103	82-83	0.37412239904979216
1103	84-85	0.4126797186424014
1103	86-87	0.459967700501263
1103	88-89	0.5179939420280846
1103	90-91	0.4710266375193797
1103	92-93	0.4451586477417955
1103	94-95	0.42417866258479364
1103	96-97	0.5766071734499967
1103	98-99	0.49152487094092834
1103	100	0.5669019485383622
1104	1	5.9966272858515595
1104	2	5.079358320714739
1104	3	4.0814204072732
1104	4	1.7944965471836056
1104	5	2.83651709627253
1104	6	1.2454594143235909
1104	7	0.681040488885337
1104	8	0.5406677858591209
1104	9	0.46356941273711527
1104	10-11	0.42402910835190255
1104	12-13	0.38552643731256353
1104	14-15	0.46747984997730896
1104	16-17	0.34162504168785546
1104	18-19	0.2727827200064752
1104	20-21	0.25321234429593176
1104	22-23	0.24860361381241347
1104	24-25	0.19214969901846501
1104	26-27	0.1984033435652819
1104	28-29	0.17787253943598813
1104	30-31	0.3074977486236605
1104	32-33	0.3714247662537318
1104	34-35	0.23409437635199026
1104	36-37	0.3216158397264337
1104	38-39	0.3165730583073554
1104	40-41	0.2375026066202679
1104	42-43	0.36922414104972745
1104	44-45	0.39669000872598303
1104	46-47	0.41165021476776786
1104	48-49	0.47086956152956105
1104	50-51	0.5136722313173863
1104	52-53	0.48290163099580496
1104	54-55	0.4587943746883454
1104	56-57	0.36900841816287766
1104	58-59	0.3586648191038506
1104	60-61	0.4288406897404826
1104	62-63	0.4135686592992798
1104	64-65	0.38769114502281354
1104	66-67	0.4107900458549878
1104	68-69	0.4263306532500586
1104	70-71	0.3638513690366736
1104	72-73	0.4628292240477343
1104	74-75	0.49652312545450883
1104	76-77	0.4538264492614914
1104	78-79	0.4714340623045601
1104	80-81	0.5247535768324383
1104	82-83	0.5420601787460448
1104	84-85	0.5637013807869451
1104	86-87	0.5093291341317645
1104	88-89	0.5467789811415145
1104	90-91	0.5643499355566632
1104	92-93	0.5905500463111615
1104	94-95	0.6458566639238121
1104	96-97	0.5984003567897886
1104	98-99	0.5693151163688839
1104	100	0.5856118660830241
1105	1	1.89130575295993
1105	2	3.315418967990336
1105	3	3.3231292260848235
1105	4	1.4263226579816788
1105	5	2.5898180248071014
1105	6	1.190421450407122
1105	7	0.732612369896465
1105	8	0.5157429688782287
1105	9	0.35411794113610995
1105	10-11	0.40005284956257015
1105	12-13	0.4225992757872703
1105	14-15	0.5621570217572867
1105	16-17	0.49694517130705407
1105	18-19	0.4802087837409488
1105	20-21	0.42854853598726095
1105	22-23	0.3698417642561722
1105	24-25	0.38993501128975794
1105	26-27	0.47005637690055835
1105	28-29	0.603648872922598
1105	30-31	0.5889353688463288
1105	32-33	0.6004971168214084
1105	34-35	0.6168399004526606
1105	36-37	0.7117776586265734
1105	38-39	0.6803123367662636
1105	40-41	0.7272395578058095
1105	42-43	0.7008794843873076
1105	44-45	0.7248517434297668
1105	46-47	0.8059004139245118
1105	48-49	0.7463370671372545
1105	50-51	0.7720119873206954
1105	52-53	0.7589706547283797
1105	54-55	0.7469091429433021
1105	56-57	0.693096345054542
1105	58-59	0.7319541760380588
1105	60-61	0.7130343909902592
1105	62-63	0.7289473917340885
1105	64-65	0.691740071735282
1105	66-67	0.7105023210345536
1105	68-69	0.6822373507455417
1105	70-71	0.7198502507364921
1105	72-73	0.6801715663900794
1105	74-75	0.7401563165176057
1105	76-77	0.7313600036642001
1105	78-79	0.7825347028444511
1105	80-81	0.7792675264398454
1105	82-83	0.7210888659523071
1105	84-85	0.666710752487905
1105	86-87	0.7073559711772894
1105	88-89	0.7289021539188951
1105	90-91	0.7401805109199486
1105	92-93	0.8249448472947485
1105	94-95	0.7908470892880679
1105	96-97	0.6683826323795437
1105	98-99	0.6927248309000973
1105	100	0.7784214981449935
1106	1	-1.079694282043043
1106	2	1.6064766624398175
1106	3	2.56356042031209
1106	4	1.1015703570664073
1106	5	2.3894175059145084
1106	6	1.0406315212934487
1106	7	0.5970601661409702
1106	8	0.4093662753441265
1106	9	0.23289521296179316
1106	10-11	0.33106145499099426
1106	12-13	0.30602637466643046
1106	14-15	0.37652987981385166
1106	16-17	0.3308955646662426
1106	18-19	0.29936299518224985
1106	20-21	0.309830111587857
1106	22-23	0.2841396310899924
1106	24-25	0.324625028123009
1106	26-27	0.28622950161616245
1106	28-29	0.3932804613045846
1106	30-31	0.3400976267291611
1106	32-33	0.2993036744697193
1106	34-35	0.37268168488311915
1106	36-37	0.40367828836191677
1106	38-39	0.5285503082059577
1106	40-41	0.4816125431459284
1106	42-43	0.45556696615659575
1106	44-45	0.5489737256164346
1106	46-47	0.5099852611365279
1106	48-49	0.4993971757313389
1106	50-51	0.4702913789477705
1106	52-53	0.37235616370876556
1106	54-55	0.4684745290314254
1106	56-57	0.4853449361847879
1106	58-59	0.4739559961927853
1106	60-61	0.3591625315791802
1106	62-63	0.5505096487683616
1106	64-65	0.48941226781134617
1106	66-67	0.5357539446952941
1106	68-69	0.5187193608072995
1106	70-71	0.5572197983601583
1106	72-73	0.5114619988041049
1106	74-75	0.5381666095137234
1106	76-77	0.5291602788088028
1106	78-79	0.4921476335799255
1106	80-81	0.408969990543234
1106	82-83	0.39663488584884377
1106	84-85	0.4134117220703182
1106	86-87	0.38026616096766475
1106	88-89	0.4464607600367785
1106	90-91	0.3871511094814295
1106	92-93	0.4174420656189106
1106	94-95	0.4187251558934264
1106	96-97	0.5222306261256762
1106	98-99	0.4090397707002822
1106	100	0.2801843752622766
1107	1	-1.0392411679558293
1107	2	1.6413123559512144
1107	3	-0.7156915482357036
1107	4	-0.09719866703068902
1107	5	1.8481902281746656
1107	6	0.7209993994055637
1107	7	0.27387858744862115
1107	8	0.2087342459103141
1107	9	0.23792003632437542
1107	10-11	0.26564434786790514
1107	12-13	0.2799632363295217
1107	14-15	0.3182593057185983
1107	16-17	0.17390659343505632
1107	18-19	0.10232637111500509
1107	20-21	0.07279373257961197
1107	22-23	0.1116295140520478
1107	24-25	0.0729822974083163
1107	26-27	0.23266266790093937
1107	28-29	0.19939117450423538
1107	30-31	0.15090094126349385
1107	32-33	0.13031210338534294
1107	34-35	0.13919002858504825
1107	36-37	0.31953031951113786
1107	38-39	0.24880682928159104
1107	40-41	0.2655824543566041
1107	42-43	0.2563186846328165
1107	44-45	0.3264732438527247
1107	46-47	0.3665767355339895
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2213	100	0.03314123053737461
2214	1	-2.333986472844394
2214	2	-2.970950681649061
2214	3	-4.230197537906388
2214	4	-2.1735216434373186
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2214	94-95	-1.9484757899976834
2214	96-97	-1.997148947663625
2214	98-99	-1.9719367639240915
2214	100	-1.9180399800345747
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2215	2	-0.9282914065525105
2215	3	-2.7816113831182463
2215	4	-1.2492474649441192
2215	5	-3.151098348622373
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2215	9	-0.4125962584624858
2215	10-11	-0.25101186889449423
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2215	14-15	-0.3522346944442063
2215	16-17	-0.42454109122719785
2215	18-19	-0.4140733632870024
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2215	22-23	-0.3827712657888753
2215	24-25	-0.3634200001354344
2215	26-27	-0.46746152001703933
2215	28-29	-0.6025719835285841
2215	30-31	-0.6130153711491744
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2215	100	-1.000896295378869
2216	1	2.951490328742768
2216	2	-1.7692513940921621
2216	3	-2.0663934412448732
2216	4	-0.7741731517677835
2216	5	-2.4070935240986557
2216	6	-1.107430040678537
2216	7	-0.4752693962977901
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2216	9	-0.2965701959940432
2216	10-11	-0.12976642607743116
2216	12-13	-0.23936036715170417
2216	14-15	-0.3109337206897891
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2216	26-27	-0.2591934731864569
2216	28-29	-0.3434671914107881
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2216	32-33	-0.3147654868379277
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2216	50-51	-0.471444250277969
2216	52-53	-0.2943447377998538
2216	54-55	0.03433863509176405
2216	56-57	0.1645604707571806
2216	58-59	0.18412462343689384
2216	60-61	0.2600875650014416
2216	62-63	0.28604229761798905
2216	64-65	0.21719358896187657
2216	66-67	0.2657141736664457
2216	68-69	0.3278345406694321
2216	70-71	0.11552666296549319
2216	72-73	0.19412566133008724
2216	74-75	0.14606337534686986
2216	76-77	0.10009473813541092
2216	78-79	0.167636765549517
2216	80-81	0.179068373752866
2216	82-83	0.24480802409952673
2216	84-85	0.19730275030380895
2216	86-87	0.013685467713447252
2216	88-89	0.11045018213665969
2216	90-91	0.13671618045811584
2216	92-93	0.05413619475646314
2216	94-95	-9.250039846193658E-4
2216	96-97	-0.08926292946504333
2216	98-99	-0.14490235136970853
2216	100	-0.0626561202131306
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	422.0
3	96.0
4	132.0
5	315.0
6	1017.0
7	1802.0
8	2230.0
9	3081.0
10	2361.0
11	2148.0
12	2070.0
13	3054.0
14	3330.0
15	3724.0
16	4533.0
17	5261.0
18	7549.0
19	13383.0
20	26917.0
21	32063.0
22	14671.0
23	9031.0
24	8551.0
25	8957.0
26	9122.0
27	9899.0
28	11662.0
29	13469.0
30	15287.0
31	18820.0
32	24010.0
33	33732.0
34	49861.0
35	97503.0
36	143178.0
37	229892.0
38	246316.0
39	63940.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.32553832772176	43.78131444470388	23.607459677778706	19.28568754979565
2	14.676453520454844	43.472553019541664	23.60869317554746	18.242300284456025
3	17.94606843175159	23.883293638502916	17.84959041343145	40.32104751631404
4	36.4418500336415	25.521443345211694	20.04446374727219	17.992242873874613
5	16.960147955510106	42.252494479703735	20.622812240506025	20.164545324280137
6	40.240956348358615	22.06953642384106	22.019867549668874	15.669639678131453
7	18.516478088401122	21.7975886071612	42.166978318468686	17.518954985968993
8	39.232671064388775	22.204907031964773	21.34936259135716	17.213059312289296
9	38.58458257832775	23.772886014033315	21.708383320932846	15.934148086706088
10-11	16.03333713061092	45.46346959626633	21.704244881685135	16.798948391437612
12-13	15.895700651114442	23.420139692349316	32.41376916586864	28.270390490667594
14-15	16.367171229926345	34.06313745314219	21.53332289081996	28.0363684261115
16-17	27.629599897473504	22.89455790788991	32.59594949146147	16.879892703175116
18-19	16.64538114580478	23.232752943186792	32.659583557313375	27.46228235369505
20-21	27.711612193532996	34.42189118663738	21.6649074591585	16.20158916067112
22-23	27.09209228808521	23.48034730850151	32.81332880033322	16.614231603080068
24-25	16.26918366838375	34.3708504359156	21.566786399575655	27.793179496125
26-27	16.77126979775937	28.24444737151259	38.42861236797044	16.555670462757607
28-29	27.22661381330922	23.192036040910917	33.19611218107318	16.38523796470668
30-31	23.760960189104424	25.155480717543067	33.65872738988192	17.42483170347059
32-33	18.823624169197195	27.014841422127922	34.6424744484751	19.519059960199783
34-35	19.208766015314897	26.699563550407078	35.09036037350972	19.001310060768308
36-37	19.552790465035976	26.13037240613308	35.791892575016995	18.52494455381395
38-39	20.55224579303176	26.034253817153964	34.90421960605618	18.509280783758093
40-41	18.352999077973536	28.27306045147257	34.71158193397721	18.662358536576683
42-43	18.497610129615037	26.456606085903616	35.64019645842613	19.405587326055215
44-45	18.52160710280773	27.482440557772613	34.36755748425613	19.628394855163524
46-47	19.58683246529941	26.359499542546715	35.288026471771396	18.76564152038248
48-49	18.67148090266035	26.600063011074997	35.15466192956288	19.57379415670178
50-51	19.587188460073406	27.674366240303595	33.976764221101234	18.761681078521768
52-53	19.68294159005349	27.100773400023137	34.10128959336425	19.11499541655913
54-55	19.657096933817012	28.62576227380982	31.418318779080323	20.298822013292845
56-57	20.14721273891699	29.50079742829375	30.316292456826734	20.03569737596252
58-59	22.029090112957142	29.38634510845381	28.683878420664783	19.900686357924265
60-61	21.016685475057226	30.377256561873672	28.438820518114795	20.16723744495431
62-63	19.752387614574797	30.513540718273497	28.165397099174	21.56867456797771
64-65	20.493818150749547	30.365152739557782	28.864590267814865	20.276438841877802
66-67	20.697990364107167	29.338832861714216	29.80714419530238	20.15603257887624
68-69	21.372591250360447	29.402587370017407	29.296945920833885	19.927875458788264
70-71	19.197863675361244	31.128361035659996	29.44450575465552	20.229269534323237
72-73	19.103652575261133	29.639521735628506	30.11829340306131	21.13853228604905
74-75	19.369130234534744	30.400206477795848	28.95477290779921	21.275890379870194
76-77	20.546082314624332	29.46973732281895	29.386389415839304	20.597790946717414
78-79	19.90912290835173	29.88330868796922	28.860485285983383	21.347083117695668
80-81	20.65990624769157	30.866706004544326	28.01221788200239	20.461169865761715
82-83	20.49091291789208	30.40208686272389	28.48672637650126	20.62027384288277
84-85	19.857279916838586	31.218549694819675	27.4150096386786	21.50916074966314
86-87	19.943014111194127	31.310065801213323	27.9906355533029	20.756284534289655
88-89	21.746964254564745	30.286655891891506	27.35499442868179	20.611385424861965
90-91	20.752546587499328	31.32884453389806	27.15573843806333	20.76287044053928
92-93	19.868602328917813	31.45022481069978	27.03135067977202	21.64982218061039
94-95	20.466940545312795	31.70071163355322	26.743128410874927	21.08921941025906
96-97	20.66949360334747	30.561576652807883	27.49953163749766	21.26939810634699
98-99	21.562283203490907	30.77249977861447	26.485007415859034	21.18020960203559
100	20.14245130881479	32.1468620840646	26.377788774060797	21.33289783305981
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27388.0
1	14885.0
2	1782.5
3	1021.5
4	869.5
5	889.0
6	885.5
7	945.5
8	922.0
9	815.5
10	817.5
11	1301.0
12	9406.0
13	10699.0
14	3963.0
15	3714.5
16	4115.0
17	4640.5
18	5136.0
19	5749.0
20	6405.0
21	7093.5
22	8060.5
23	9074.0
24	10124.5
25	11180.5
26	12452.5
27	13783.0
28	14866.5
29	15901.0
30	17199.0
31	18510.0
32	19739.5
33	21115.5
34	22444.0
35	23902.0
36	25350.5
37	26432.5
38	27174.0
39	27845.5
40	28445.0
41	28900.5
42	29432.5
43	29877.0
44	30397.5
45	30982.0
46	31670.0
47	33298.5
48	34893.5
49	39473.0
50	55030.5
51	55700.0
52	38433.5
53	30478.0
54	28296.5
55	27372.0
56	25092.5
57	21810.0
58	19045.0
59	16036.0
60	13281.0
61	11034.5
62	9148.0
63	7300.0
64	5686.5
65	4338.0
66	3262.5
67	2582.5
68	2052.0
69	1540.0
70	1104.0
71	806.5
72	595.5
73	426.0
74	295.5
75	211.0
76	155.0
77	97.0
78	66.5
79	37.0
80	11.0
81	4.5
82	2.0
83	2.0
84	1.5
85	1.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13581200627974782
2	0.005695916383947484
3	0.0035599477399671773
4	0.0
5	0.0027589594984745625
6	0.015307775281858864
7	0.0025809621114762037
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0013794797492372812
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.7799738699835887E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	8.899869349917943E-5
64-65	0.0
66-67	4.4499346749589716E-5
68-69	0.0
70-71	0.0
72-73	6.674902012438458E-4
74-75	4.004941207463074E-4
76-77	7.564888947430253E-4
78-79	5.784915077446663E-4
80-81	0.0011569830154893326
82-83	0.0010234849752405635
84-85	0.0012459817089885122
86-87	4.4499346749589716E-5
88-89	0.0
90-91	4.4499346749589716E-5
92-93	0.0
94-95	0.0
96-97	0.0014684784427364606
98-99	5.784915077446663E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1123612.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.1450698746357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.41526401681207	64.59204957800011
2	4.262767243173089	5.894986777770227
3	0.8143933878365789	1.6893386312220462
4	0.3566013711158477	0.9862890689278474
5	0.21836995172387813	0.7549602785234188
6	0.16996279975905532	0.7051253799257157
7	0.1384873385985951	0.6703001692906535
8	0.10997300420320477	0.608327284796336
9	0.09228003031774987	0.5742638229918884
>10	0.38372639297384803	4.347409050236674
>50	0.01735763721385142	0.819337801965999
>100	0.014777377808400806	2.2489881142840282
>500	0.004497461621966481	2.238325741134605
>1k	0.001027991227878053	1.399575542805347
>5k	1.2849890348475663E-4	0.6617596903767282
>10k+	3.854967104542699E-4	11.808963067748415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	93650	8.334727646198154	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25509	2.270267672470568	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	13749	1.223643036920218	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7448	0.6628622691818884	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3041	0.2706450269310047	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2553	0.2272136645034051	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2198	0.19561912831119638	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2130	0.1895672171532522	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2080	0.1851172824782932	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	1550	0.13794797492372812	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	1155	0.10279349099155223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5599477399671773E-4	0.0	0.0	0.0	0.0
2	3.5599477399671773E-4	0.0	0.0	0.0	0.0
3	3.5599477399671773E-4	0.0	0.0	0.0	0.0
4	3.5599477399671773E-4	0.0	0.0	0.0	0.0
5	3.5599477399671773E-4	0.0	0.0	0.0	0.0
6	3.5599477399671773E-4	0.0	0.0	0.0	0.0
7	3.5599477399671773E-4	0.0	0.0	0.0	0.0
8	3.5599477399671773E-4	0.0	0.0	0.0	0.0
9	3.5599477399671773E-4	0.0	0.0	0.0	0.0
10-11	3.5599477399671773E-4	0.0	0.0	0.0	0.0
12-13	3.5599477399671773E-4	0.0	0.0	0.0	0.0
14-15	3.5599477399671773E-4	0.0	0.0	0.0	0.0
16-17	3.5599477399671773E-4	0.0	0.0	0.0	0.0
18-19	3.5599477399671773E-4	0.0	0.0	0.0	0.0
20-21	3.5599477399671773E-4	0.0	0.0	0.0	0.0
22-23	4.004941207463074E-4	0.0	0.0	0.0	0.0
24-25	5.784915077446663E-4	0.0	0.0	0.0	0.0
26-27	7.119895479934355E-4	0.0	0.0	0.0	0.0
28-29	9.789856284909738E-4	0.0	0.0	0.0	0.0
30-31	0.0010234849752405635	0.0	0.0	0.0	0.0
32-33	0.0012014823622389223	0.0	0.0	0.0	0.0
34-35	0.0018244732167331783	0.0	0.0	0.0	0.0
36-37	0.002669960804975383	0.0	0.0	0.0	0.0
38-39	0.0044499346749589715	0.0	0.0	0.0	0.0
40-41	0.008410376535672457	0.0	0.0	0.0	0.0
42-43	0.012326319049636351	0.0	0.0	0.0	0.0
44-45	0.016420258950598607	0.0	0.0	0.0	0.0
46-47	0.020736695585308808	0.0	0.0	0.0	0.0
48-49	0.024875134833020653	0.0	0.0	0.0	0.0
50-51	0.02972556362872593	0.0	0.0	0.0	0.0
52-53	0.03795794277740003	0.0	0.0	0.0	0.0
54-55	0.05037326052053556	0.0	0.0	0.0	0.0
56-57	0.06243258348967437	0.0	0.0	0.0	0.0
58-59	0.07507039796655784	0.0	0.0	0.0	0.0
60-61	0.09322613144039046	0.0	0.0	0.0	0.0
62-63	0.1181457656201607	0.0	0.0	0.0	0.0
64-65	0.1427984037194334	0.0	0.0	0.0	0.0
66-67	0.17474893468563882	0.0	0.0	0.0	0.0
68-69	0.2129738735435364	0.0	0.0	0.0	0.0
70-71	0.26534960466780344	0.0	0.0	0.0	0.0
72-73	0.32520122604600166	0.0	0.0	0.0	0.0
74-75	0.3808254094829888	0.0	0.0	0.0	0.0
76-77	0.44797492372811965	0.0	0.0	0.0	0.0
78-79	0.5192183778742128	0.0	0.0	0.0	0.0
80-81	0.6129340021288487	0.0	0.0	0.0	0.0
82-83	0.72560634809881	0.0	0.0	0.0	0.0
84-85	0.8633763256355397	0.0	0.0	0.0	0.0
86-87	1.0184565490578597	0.0	0.0	0.0	0.0
88	1.1415862415139746	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	1795	0.0	87.60783	1
AACGTGT	915	0.0	76.67011	1
ATCGAGT	715	0.0	75.72734	1
CGTGTGG	1045	0.0	72.416145	3
TCGAGTG	810	0.0	71.38132	2
ACGTGTG	1025	0.0	69.70825	2
GTGGAAT	36430	0.0	64.02114	6
TGGAATC	36730	0.0	63.479786	7
GGAATCA	37040	0.0	62.89775	8
GAATCAC	37125	0.0	62.69044	9
AGTGGAA	36090	0.0	62.462322	5
ACGAGTG	34470	0.0	61.639862	2
CGAGTGG	35795	0.0	61.506405	3
GAGTGGA	36885	0.0	61.052322	4
AACGAGT	32115	0.0	60.97361	1
AGCGAGT	380	0.0	59.47282	1
TGAGTGG	1110	0.0	54.625137	3
GCGAGTG	435	0.0	54.031273	2
GTGTGGA	1585	0.0	50.41334	4
AATGAGT	1080	0.0	50.1343	1
>>END_MODULE