FastQCFastQC Report
Thu 26 May 2016
SRR2132778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294008
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1338405.834330133112004No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188230.8205289606662227No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107420.4682634062304927No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG71000.309501972094256No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44110.19228354914193846No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37260.1624231476089011No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31250.13622445954852816No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29590.12898821625731033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT26250.088.9601441
AACGAGT347550.077.458661
ACGAGTG381700.076.8021242
CGAGTGG396400.076.181893
GTGGAAT420650.076.141816
TGGAATC421900.075.822137
GAATCAC422250.075.754339
GGAATCA424100.075.4127968
AGTGGAA414400.074.5938345
GAGTGGA414850.074.546924
ATCGAGT9750.068.085971
AACGTGT14800.066.485621
CGTGTGG17350.062.8591273
ACGTGTG17100.060.480572
TCGAGTG11250.060.1726722
GCGAGTG5750.044.1484262
GTGTGGA27100.042.3234864
GAGTACT218350.041.52065320-21
AGTACTT222950.041.34909420-21
TGAGTGG21250.040.7041553
Produced by FastQC (version 0.11.5)