##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132778_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2294008 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.767015197854583 31.0 23.0 33.0 16.0 34.0 2 28.06802243061053 31.0 25.0 33.0 16.0 34.0 3 29.146139856530578 31.0 28.0 34.0 16.0 34.0 4 34.67936031609305 35.0 35.0 37.0 32.0 37.0 5 33.784393515628544 35.0 35.0 37.0 28.0 37.0 6 35.21608032753155 37.0 35.0 37.0 32.0 37.0 7 35.63318567328449 37.0 35.0 37.0 33.0 37.0 8 35.90397723111689 37.0 35.0 37.0 35.0 37.0 9 37.86864954263455 39.0 38.0 39.0 35.0 39.0 10-11 37.86970359301275 39.0 38.0 39.0 35.0 39.0 12-13 37.850495290339005 39.0 38.0 39.0 35.0 39.0 14-15 39.2720877608099 41.0 39.0 41.0 36.0 41.0 16-17 39.289701692409096 41.0 39.0 41.0 36.0 41.0 18-19 39.25990558882096 41.0 39.0 41.0 36.0 41.0 20-21 39.25516933681138 40.5 39.0 41.0 36.0 41.0 22-23 39.164256837813994 40.0 39.0 41.0 36.0 41.0 24-25 39.087728333990114 40.0 39.0 41.0 36.0 41.0 26-27 38.94182430924391 40.0 39.0 41.0 35.0 41.0 28-29 38.785162693416936 40.0 39.0 41.0 35.0 41.0 30-31 38.67686032481142 40.0 39.0 41.0 35.0 41.0 32-33 38.533579438258286 40.0 38.0 41.0 35.0 41.0 34-35 38.453491007877915 40.0 38.0 41.0 34.5 41.0 36-37 38.32589446069935 40.0 38.0 41.0 34.0 41.0 38-39 37.92957522380044 40.0 37.0 41.0 34.0 41.0 40-41 37.71353827013681 40.0 36.5 41.0 33.0 41.0 42-43 37.58076018043529 40.0 36.0 41.0 33.0 41.0 44-45 37.45200866779889 40.0 36.0 41.0 33.0 41.0 46-47 37.33252564943104 40.0 35.5 41.0 33.0 41.0 48-49 37.19301610979561 40.0 35.0 41.0 33.0 41.0 50-51 36.96150318569072 40.0 35.0 41.0 32.5 41.0 52-53 36.62200044638031 40.0 35.0 41.0 31.5 41.0 54-55 35.91862822623112 39.5 35.0 41.0 29.0 41.0 56-57 35.32514359147832 39.0 35.0 41.0 25.5 41.0 58-59 35.01677587872405 39.0 35.0 41.0 24.5 41.0 60-61 34.784214789137614 39.0 35.0 41.0 24.0 41.0 62-63 34.52255201376804 38.5 35.0 40.0 23.0 41.0 64-65 34.257794436636665 38.0 35.0 40.0 23.0 41.0 66-67 33.963210241638215 37.0 34.5 40.0 22.0 41.0 68-69 33.64706574693724 37.0 34.0 40.0 21.5 41.0 70-71 33.30996557117499 36.0 34.0 39.0 20.5 41.0 72-73 32.973474591195846 36.0 34.0 39.0 19.5 40.5 74-75 32.61872037935352 35.0 34.0 38.5 18.5 40.0 76-77 32.271650534784534 35.0 33.5 37.0 16.0 39.5 78-79 31.923725418568722 35.0 33.0 37.0 12.0 39.0 80-81 31.6049752224055 35.0 33.0 36.5 7.5 39.0 82-83 31.302343976132605 35.0 33.0 36.0 4.5 37.5 84-85 31.01762417567855 35.0 33.0 36.0 2.0 37.0 86-87 30.79295865576755 35.0 33.0 35.0 2.0 37.0 88-89 30.579708963525846 35.0 33.0 35.0 2.0 36.0 90-91 30.386731432497186 35.0 33.0 35.0 2.0 36.0 92-93 30.21415029938867 35.0 33.0 35.0 2.0 36.0 94-95 30.040735908505987 35.0 32.0 35.0 2.0 36.0 96-97 29.872028345149623 35.0 32.0 35.0 2.0 35.0 98-99 29.71329720733319 35.0 32.0 35.0 2.0 35.0 100 29.61221146569672 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26808401438847085 1101 2 2.3348419979558024 1101 3 1.2386532675863506 1101 4 0.6557680712352294 1101 5 2.2308797007153913 1101 6 0.9853084383638873 1101 7 0.5248519032273862 1101 8 0.3868916323190845 1101 9 0.3089474467817155 1101 10-11 0.4505564436257785 1101 12-13 0.33231658797673447 1101 14-15 0.514641055937723 1101 16-17 0.4561376184848598 1101 18-19 0.4053346474256898 1101 20-21 0.4342889237279479 1101 22-23 0.36414993428581255 1101 24-25 0.38527322623459526 1101 26-27 0.3928130089943309 1101 28-29 0.41936534371080825 1101 30-31 0.38824875700777994 1101 32-33 0.4703722181427139 1101 34-35 0.44193205873883556 1101 36-37 0.5230773176035513 1101 38-39 0.5177108436765963 1101 40-41 0.4274295346011172 1101 42-43 0.5113231203526212 1101 44-45 0.5602330361313079 1101 46-47 0.4986526216689384 1101 48-49 0.5018371161814983 1101 50-51 0.6451878844791707 1101 52-53 0.6564122422193819 1101 54-55 0.5540779876650248 1101 56-57 0.41178727274124327 1101 58-59 0.4276811656543984 1101 60-61 0.45329736481609473 1101 62-63 0.48852017105758705 1101 64-65 0.5152106341732292 1101 66-67 0.4916707259805406 1101 68-69 0.46444872501825785 1101 70-71 0.48188444165758426 1101 72-73 0.5562811383936435 1101 74-75 0.5801053060418866 1101 76-77 0.4721558097091645 1101 78-79 0.5657577554750759 1101 80-81 0.5587847026051378 1101 82-83 0.5728960234668712 1101 84-85 0.5723911729226536 1101 86-87 0.5913239321907149 1101 88-89 0.5400724397840122 1101 90-91 0.5671498182166488 1101 92-93 0.5705011454414617 1101 94-95 0.5218629753983457 1101 96-97 0.5623491713634365 1101 98-99 0.6268452876448514 1101 100 0.6329900851150256 1102 1 1.4894669507592866 1102 2 3.106267282553837 1102 3 3.3170701999805488 1102 4 1.4705620954785132 1102 5 2.7286013194535883 1102 6 1.2654544334002438 1102 7 0.755508981232218 1102 8 0.5149865047847086 1102 9 0.4259589268045616 1102 10-11 0.5179905367681386 1102 12-13 0.4601026639248431 1102 14-15 0.6401946461247476 1102 16-17 0.5512150416153787 1102 18-19 0.5566093269450789 1102 20-21 0.4965086308249127 1102 22-23 0.4838131779042172 1102 24-25 0.5484632822235653 1102 26-27 0.5898900556333473 1102 28-29 0.6066026281839143 1102 30-31 0.6062840776763565 1102 32-33 0.5833802961748233 1102 34-35 0.6010216220242413 1102 36-37 0.573799159494925 1102 38-39 0.672395039527359 1102 40-41 0.6891842944839155 1102 42-43 0.7188637051891931 1102 44-45 0.747755586403315 1102 46-47 0.8580708871878642 1102 48-49 0.7747209520193366 1102 50-51 0.9036661507866981 1102 52-53 0.9534762233032268 1102 54-55 0.8699761184497419 1102 56-57 0.8425587326506161 1102 58-59 0.8312660247039787 1102 60-61 0.7333967930549647 1102 62-63 0.6916275119775648 1102 64-65 0.7085165121750023 1102 66-67 0.7186311139589847 1102 68-69 0.7073436139341851 1102 70-71 0.6767799857591683 1102 72-73 0.732681113689921 1102 74-75 0.7237377310612132 1102 76-77 0.6856979997223362 1102 78-79 0.7245196552703916 1102 80-81 0.6655794696559667 1102 82-83 0.6446351428413877 1102 84-85 0.6723641867922119 1102 86-87 0.6412431504564324 1102 88-89 0.6613038680053549 1102 90-91 0.6892934680953857 1102 92-93 0.693604891037225 1102 94-95 0.7684681569332454 1102 96-97 0.8562649472192803 1102 98-99 0.8933139288285261 1102 100 0.8132177946348769 1103 1 1.4092499254133841 1103 2 3.018118096827802 1103 3 3.2952371511641445 1103 4 1.4527909189836592 1103 5 2.635749332389814 1103 6 1.2302004780430593 1103 7 0.7562898198647545 1103 8 0.44635460841504937 1103 9 0.38663676744975106 1103 10-11 0.45735044108221246 1103 12-13 0.45598207041395256 1103 14-15 0.5702821172083858 1103 16-17 0.44235453497508104 1103 18-19 0.3583009261181189 1103 20-21 0.3246007661070962 1103 22-23 0.3581494242170393 1103 24-25 0.32565272474604257 1103 26-27 0.4113673255290067 1103 28-29 0.4619745855582522 1103 30-31 0.5032057188958063 1103 32-33 0.49008110895778145 1103 34-35 0.46825454477902184 1103 36-37 0.42955214079524495 1103 38-39 0.45235785829150643 1103 40-41 0.5019101668152643 1103 42-43 0.5631001847119421 1103 44-45 0.6464513224075574 1103 46-47 0.5692367775093459 1103 48-49 0.6358979329863175 1103 50-51 0.7096271378582344 1103 52-53 0.7281118557702655 1103 54-55 0.7418815117714175 1103 56-57 0.6785608674466914 1103 58-59 0.6977640645396121 1103 60-61 0.7240453159974862 1103 62-63 0.6666090173560164 1103 64-65 0.6594315210659047 1103 66-67 0.716069848865601 1103 68-69 0.6942143729877657 1103 70-71 0.6103380323186371 1103 72-73 0.7083309172649876 1103 74-75 0.65024096287123 1103 76-77 0.6289315621806395 1103 78-79 0.5965166536474413 1103 80-81 0.5762205253056791 1103 82-83 0.6426456689464786 1103 84-85 0.6439722364358182 1103 86-87 0.5975412073671684 1103 88-89 0.5648055503929612 1103 90-91 0.5854951364716037 1103 92-93 0.6617895464041013 1103 94-95 0.7265589972855508 1103 96-97 0.6486692030859871 1103 98-99 0.687114319268268 1103 100 0.7560940258905617 1104 1 6.127298179453035 1104 2 5.187885369793609 1104 3 4.225062555129291 1104 4 1.9074516288628018 1104 5 2.90882173219115 1104 6 1.3403905048751525 1104 7 0.7441892166892572 1104 8 0.5891015870711911 1104 9 0.5108121256535298 1104 10-11 0.5105919627337414 1104 12-13 0.4567087079057899 1104 14-15 0.587516623157363 1104 16-17 0.5193039225848395 1104 18-19 0.5252844473385849 1104 20-21 0.4936168028911112 1104 22-23 0.5091637101990614 1104 24-25 0.4976686303370528 1104 26-27 0.6184797048392454 1104 28-29 0.5783513781389757 1104 30-31 0.6614379633271383 1104 32-33 0.6636357992641777 1104 34-35 0.6282897170010884 1104 36-37 0.6335443910365441 1104 38-39 0.7156259231008519 1104 40-41 0.7052692936320497 1104 42-43 0.7763380282736421 1104 44-45 0.6515399323551065 1104 46-47 0.6144310134506128 1104 48-49 0.680135162752137 1104 50-51 0.6578355723195131 1104 52-53 0.6750899652789428 1104 54-55 0.6785418374424523 1104 56-57 0.8824037684990458 1104 58-59 0.9862200164854897 1104 60-61 0.9433867583084776 1104 62-63 0.986953461488099 1104 64-65 0.9826383937055994 1104 66-67 0.9443363206169337 1104 68-69 0.9460130057691529 1104 70-71 0.9836491175035036 1104 72-73 0.9813935556814215 1104 74-75 0.981494730765796 1104 76-77 0.9679063903021756 1104 78-79 0.9503063356293353 1104 80-81 0.9835611648403813 1104 82-83 0.9095974851887974 1104 84-85 0.9635494357148815 1104 86-87 0.9669313394660399 1104 88-89 0.9192882907026387 1104 90-91 0.990489724759815 1104 92-93 1.0156303780428573 1104 94-95 1.0099593327648293 1104 96-97 0.9114288949698235 1104 98-99 0.890921493651895 1104 100 0.9602389441916763 1105 1 1.731249985690397 1105 2 3.2325530337022386 1105 3 3.380667799209725 1105 4 1.4727509741782825 1105 5 2.6317604945239523 1105 6 1.2590881693578453 1105 7 0.7153279394981595 1105 8 0.4790825693672289 1105 9 0.4183578766051781 1105 10-11 0.4004097755682494 1105 12-13 0.40472480348252304 1105 14-15 0.5088317620320737 1105 16-17 0.4284207868456207 1105 18-19 0.40921711013719175 1105 20-21 0.36074161409842986 1105 22-23 0.36721069208157076 1105 24-25 0.452929526208834 1105 26-27 0.42036410097443166 1105 28-29 0.4419819688283013 1105 30-31 0.5005457302316074 1105 32-33 0.5024869211486873 1105 34-35 0.5087725792226863 1105 36-37 0.5161321678880313 1105 38-39 0.5450080725205169 1105 40-41 0.46636402323621695 1105 42-43 0.4689834068979195 1105 44-45 0.5494647813082523 1105 46-47 0.6261443836051868 1105 48-49 0.5500454122455167 1105 50-51 0.5845292706952208 1105 52-53 0.6325205482463758 1105 54-55 0.6595966532371804 1105 56-57 0.6485529730169617 1105 58-59 0.6230316566310066 1105 60-61 0.572995573256847 1105 62-63 0.6288253552673453 1105 64-65 0.6515213582422916 1105 66-67 0.6214181457678549 1105 68-69 0.6877817271708366 1105 70-71 0.7537827812018207 1105 72-73 0.7295719253356054 1105 74-75 0.7342503546668908 1105 76-77 0.6076062791475465 1105 78-79 0.6497912421207559 1105 80-81 0.5703461720720355 1105 82-83 0.5442695701357252 1105 84-85 0.5912778805813232 1105 86-87 0.5999079098681257 1105 88-89 0.600728159755576 1105 90-91 0.5627256613874501 1105 92-93 0.6866020005905824 1105 94-95 0.7184034778165405 1105 96-97 0.751584864527274 1105 98-99 0.61111390343655 1105 100 0.6436896807943171 1106 1 -0.06014950582157752 1106 2 2.2421865217140393 1106 3 2.931586910412584 1106 4 1.2979130255778202 1106 5 2.512302558556847 1106 6 1.1509916528096937 1106 7 0.6471739538202215 1106 8 0.44115975951279296 1106 9 0.25237239988233995 1106 10-11 0.4329026792423605 1106 12-13 0.40660576479048416 1106 14-15 0.44712862036112 1106 16-17 0.40687823632295306 1106 18-19 0.3527280624125808 1106 20-21 0.407320501484719 1106 22-23 0.3796475888319648 1106 24-25 0.4238109693150065 1106 26-27 0.4026387293401612 1106 28-29 0.4532237532045116 1106 30-31 0.520119026699625 1106 32-33 0.48424381518318427 1106 34-35 0.5145586537819042 1106 36-37 0.5529141768106172 1106 38-39 0.6827473637832426 1106 40-41 0.6957830817240733 1106 42-43 0.6826544277327926 1106 44-45 0.705765390808395 1106 46-47 0.6827638178878175 1106 48-49 0.7468954593632162 1106 50-51 0.7492525846849531 1106 52-53 0.7776762673292197 1106 54-55 0.8851148664995492 1106 56-57 0.881807227326405 1106 58-59 0.8145832592779385 1106 60-61 0.8241958007458834 1106 62-63 0.8420313241958652 1106 64-65 0.8561620836528903 1106 66-67 0.8806901873841682 1106 68-69 0.815654483182989 1106 70-71 0.8319358389914129 1106 72-73 0.8879636719772535 1106 74-75 0.8496554906592237 1106 76-77 0.8282829873385111 1106 78-79 0.9184296128843457 1106 80-81 0.9027646166157837 1106 82-83 0.9497577560449315 1106 84-85 0.8719626400282223 1106 86-87 0.8647886586218938 1106 88-89 0.8513029096981342 1106 90-91 0.8899923707734736 1106 92-93 0.8863850284961714 1106 94-95 0.9292482510879339 1106 96-97 0.9146233509120094 1106 98-99 0.8704689272026123 1106 100 0.8400193219921412 1107 1 -1.0273635975472004 1107 2 1.6147116459823252 1107 3 -0.678730610716368 1107 4 -0.015348258298743644 1107 5 1.9122706933345768 1107 6 0.8646339815006812 1107 7 0.31372690299519235 1107 8 0.25340974826266205 1107 9 0.23214953792321324 1107 10-11 0.28532169961785314 1107 12-13 0.21932065740347184 1107 14-15 0.3274801837637895 1107 16-17 0.244853614965713 1107 18-19 0.23459869690753976 1107 20-21 0.17289462887019624 1107 22-23 0.19538025177940455 1107 24-25 0.1510179096090667 1107 26-27 0.2347530962986113 1107 28-29 0.18289051838579695 1107 30-31 0.18198584223282666 1107 32-33 0.21822515578470814 1107 34-35 0.2584261187818484 1107 36-37 0.2742750042361166 1107 38-39 0.27181856552780204 1107 40-41 0.2814582656484319 1107 42-43 0.3829604919764904 1107 44-45 0.4005947720028118 1107 46-47 0.4097889318181558 1107 48-49 0.39717130029494285 1107 50-51 0.5061306685877867 1107 52-53 0.6072523625746129 1107 54-55 0.7003023544132674 1107 56-57 0.7231999078118747 1107 58-59 0.7147052471326703 1107 60-61 0.6994696044925917 1107 62-63 0.7191790340958519 1107 64-65 0.563804446398315 1107 66-67 0.6097576553030635 1107 68-69 0.6245315624799588 1107 70-71 0.6391284399354475 1107 72-73 0.6302850047705846 1107 74-75 0.7065861156919127 1107 76-77 0.6926906894789511 1107 78-79 0.7281325122547031 1107 80-81 0.7204569299214683 1107 82-83 0.7882843908707002 1107 84-85 0.7267232069372831 1107 86-87 0.7173174437420506 1107 88-89 0.6693914098799603 1107 90-91 0.6850652411853986 1107 92-93 0.6794112881150305 1107 94-95 0.6342681182026624 1107 96-97 0.7183417912674912 1107 98-99 0.7052617363652018 1107 100 0.7451722446533005 1108 1 -1.6481882086610362 1108 2 0.12304406670820711 1108 3 2.022541920722432 1108 4 0.8869794571489962 1108 5 0.7357279356824193 1108 6 0.5264797730796005 1108 7 0.28362743088090525 1108 8 0.17517705140484452 1108 9 0.1703844753896746 1108 10-11 0.23033510760457432 1108 12-13 0.22684054280514943 1108 14-15 0.36922169473123034 1108 16-17 0.20239481552074778 1108 18-19 0.23731249775603658 1108 20-21 0.19425646157057486 1108 22-23 0.16945223489847194 1108 24-25 0.1605082928244812 1108 26-27 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-0.7151939700704162 2215 66-67 -0.6960453270141684 2215 68-69 -0.775508025801912 2215 70-71 -0.6732395991249973 2215 72-73 -0.628259466066055 2215 74-75 -0.5975508597633592 2215 76-77 -0.6365023693756235 2215 78-79 -0.7249553737310421 2215 80-81 -0.7480373957581357 2215 82-83 -0.5657173246215095 2215 84-85 -0.5131322958338949 2215 86-87 -0.5940443931542312 2215 88-89 -0.5994625093599453 2215 90-91 -0.6575713383870472 2215 92-93 -0.6149605778126528 2215 94-95 -0.604779123813703 2215 96-97 -0.6972856830001248 2215 98-99 -0.7832906521278034 2215 100 -0.7564772670912241 2216 1 2.9609805870202983 2216 2 -2.0397931881133395 2216 3 -2.1927993689739402 2216 4 -0.8462113789607031 2216 5 -2.3231880721758387 2216 6 -1.107006431146587 2216 7 -0.5667613404665346 2216 8 -0.3973863626742329 2216 9 -0.40767979793479725 2216 10-11 -0.20943971139902118 2216 12-13 -0.2649182203318077 2216 14-15 -0.30589287802236953 2216 16-17 -0.4349302494353111 2216 18-19 -0.4297059890874948 2216 20-21 -0.42341673246609446 2216 22-23 -0.379006270022586 2216 24-25 -0.41460772741785235 2216 26-27 -0.5713901760363314 2216 28-29 -0.5922240112946042 2216 30-31 -0.6037056871564204 2216 32-33 -0.5578873910774433 2216 34-35 -0.5288943973195899 2216 36-37 -0.49670308063620183 2216 38-39 -0.5715413962816243 2216 40-41 -0.47112658683766284 2216 42-43 -0.5448163246290463 2216 44-45 -0.5806264253695019 2216 46-47 -0.5711119749277174 2216 48-49 -0.6246583231137635 2216 50-51 -0.5573274249030362 2216 52-53 -0.6074560958612949 2216 54-55 -0.46619070051652756 2216 56-57 -0.40629822811470717 2216 58-59 -0.31205899453647135 2216 60-61 -0.2740630158912296 2216 62-63 -0.1990546436975862 2216 64-65 -0.27678093152861294 2216 66-67 -0.28152455247754204 2216 68-69 -0.2455744187747797 2216 70-71 -0.25171683496181885 2216 72-73 -0.2877298060927487 2216 74-75 -0.3662346965707286 2216 76-77 -0.324378728383067 2216 78-79 -0.2945253212390284 2216 80-81 -0.26934463206353243 2216 82-83 -0.2708260001621987 2216 84-85 -0.23533541976384242 2216 86-87 -0.28166611660815377 2216 88-89 -0.343490400776318 2216 90-91 -0.3913779889781104 2216 92-93 -0.3096023128727339 2216 94-95 -0.39348643124584726 2216 96-97 -0.3853398588730208 2216 98-99 -0.3853510750195639 2216 100 -0.47269867071508287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1091.0 3 273.0 4 271.0 5 457.0 6 975.0 7 1655.0 8 2143.0 9 2763.0 10 2557.0 11 4014.0 12 3449.0 13 4960.0 14 4960.0 15 5340.0 16 6726.0 17 7728.0 18 10999.0 19 19693.0 20 39060.0 21 46604.0 22 21732.0 23 14133.0 24 13543.0 25 14437.0 26 15672.0 27 17656.0 28 21151.0 29 25058.0 30 29497.0 31 37031.0 32 47855.0 33 65750.0 34 97494.0 35 167991.0 36 278552.0 37 491844.0 38 595337.0 39 173037.0 40 520.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.404515324997327 39.354959807758206 25.64520388586844 20.59532098137603 2 15.818353658377093 38.604751936233974 25.917591371149218 19.659303034239713 3 19.082362839400417 25.677013516076087 19.854660223589597 35.3859634209339 4 30.94025827285694 26.713899864342235 22.58749751526586 19.75834434753497 5 18.408356869687427 37.190868751931696 22.444679941184905 21.95609443719597 6 34.62864482106469 23.637091310500693 24.21497000088066 17.519293867553955 7 19.689566167716745 23.063962569383566 37.96580659307892 19.28066466982076 8 33.67159138067522 23.359421588765166 24.113080686728207 18.855906343831407 9 33.35820973597302 24.861988275542195 23.881738860544512 17.89806312794027 10-11 17.847235057593522 39.866186168487644 23.965958270415797 18.320620503503037 12-13 17.759659077038965 24.724325285700836 31.368112055406954 26.147903581853242 14-15 17.96223029736601 32.09086454798762 23.909637629860054 26.037267524786312 16-17 25.708694128355265 24.39989311284006 31.461747299922234 18.429665458882443 18-19 18.177334167971516 24.504448110032744 31.55852115598551 25.75969656601023 20-21 25.832800059982354 32.2305763537006 23.84374858326562 18.09287500305143 22-23 25.506929356828746 24.69023647694341 31.55684287064387 18.245991295583973 24-25 18.09712520618934 32.083366753734076 23.84893601068523 25.970572029391352 26-27 18.354513149038713 27.657161614083297 35.72539851648294 18.262926720395047 28-29 24.97153453693274 24.444879878361366 32.35542334638764 18.228162238318262 30-31 23.39771700883345 25.30858654372609 32.637222712388095 18.656473735052362 32-33 20.17885290722613 26.740229327883775 33.35023243162186 19.730685333268237 34-35 19.94905859090291 26.449776984212786 33.737545815010236 19.863618609874074 36-37 19.982667889562723 26.44836024983348 33.82390122440724 19.74507063619656 38-39 20.39851212375894 26.322728604259442 33.579285686885136 19.699473585096477 40-41 19.826238618173956 27.012285920537334 33.467189303611846 19.694286157676867 42-43 19.980849951765244 26.53312635762421 33.595671121352375 19.89035256925818 44-45 19.822446129220125 26.828393798103583 33.29133987326984 20.057820199406454 46-47 20.208844084240337 26.498425463206754 33.48650048299744 19.806229969555467 48-49 19.926870350931644 26.696550317174133 33.394957646180835 19.98162168571339 50-51 20.244807341561145 26.986828293536902 32.87109286454101 19.89727150036094 52-53 20.335347931816205 27.01065886720847 32.590309831428044 20.063683369547284 54-55 20.691536385226208 27.633643823386837 31.155165980240696 20.519653811146256 56-57 20.964159671631485 28.232442955735117 30.08097617793835 20.72242119469505 58-59 21.44635066660622 28.711277380026573 29.205216372392773 20.637155580974433 60-61 21.23658679481501 28.96735756806428 29.01975494418502 20.776300692935685 62-63 20.686571425769095 29.176343146219008 28.864923065857457 21.27216236215444 64-65 20.96067232546704 29.017488169178137 29.285316354607303 20.736523150747512 66-67 20.925411098263627 28.689858250245475 29.52499065500004 20.859739996490855 68-69 21.101234171807594 28.716203256483848 29.466113457320116 20.716449114388443 70-71 20.41411799784482 29.168621033579655 29.50321010214437 20.914050866431154 72-73 20.407275412538052 28.787439235518914 29.66143642950383 21.143848922439208 74-75 20.552763008592404 28.97171450952767 29.275855349796842 21.19966713208309 76-77 20.823984666078758 28.812283701954534 29.382597812815085 20.98113381915162 78-79 20.745688103148996 29.015079324255165 29.03367128712181 21.20556128547403 80-81 20.884028356736906 29.345996872258457 28.69385731177698 21.076117459227657 82-83 20.90590451064709 29.344108185608135 28.671130372123425 21.07885693162135 84-85 20.8446936862339 29.543921382035016 28.319891263195686 21.2914936685354 86-87 20.81436089463526 29.697134817562716 28.280116782530136 21.20838750527189 88-89 21.283862131256733 29.466767334725947 28.107617758961606 21.14175277505571 90-91 21.000136660467177 29.842090682829365 28.002906704928694 21.154865951774767 92-93 20.793279709573813 29.86031434938326 27.894061398216568 21.452344542826353 94-95 20.92237690539876 30.063888181732583 27.718909437107452 21.2948254757612 96-97 20.910660628260977 29.742090503722128 27.857617079115233 21.48963178890166 98-99 21.249052961777583 29.81398382035873 27.407678485645558 21.52928473221813 100 20.917756171730876 30.217898106719765 27.303043406997705 21.56130231455165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19956.0 1 11027.5 2 1618.5 3 990.0 4 832.5 5 808.5 6 791.5 7 819.5 8 801.0 9 778.5 10 828.0 11 1305.5 12 10229.0 13 12077.0 14 5137.0 15 5097.5 16 6023.0 17 7048.5 18 8079.5 19 9393.5 20 10736.5 21 12391.0 22 14133.5 23 16142.5 24 18839.5 25 21807.5 26 25040.0 27 28551.0 28 31826.0 29 34986.0 30 38292.0 31 41454.0 32 45061.0 33 49180.5 34 52845.0 35 56335.0 36 59891.0 37 63230.0 38 65949.0 39 67928.0 40 69593.5 41 71766.0 42 73760.5 43 74773.0 44 75958.0 45 76813.5 46 77292.5 47 78068.0 48 77729.5 49 77572.5 50 82479.5 51 79910.5 52 68532.0 53 62074.0 54 58247.0 55 54820.0 56 50321.0 57 44895.5 58 39983.5 59 33214.5 60 26327.5 61 21429.5 62 17018.5 63 13083.5 64 9947.0 65 7691.5 66 5858.0 67 4445.5 68 3407.5 69 2525.0 70 1795.5 71 1307.0 72 976.5 73 712.0 74 483.5 75 335.5 76 241.5 77 142.5 78 80.5 79 48.5 80 25.5 81 14.5 82 8.0 83 7.0 84 3.5 85 1.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13700911243552769 2 0.005318202900774539 3 0.003182203375053618 4 0.0 5 0.0017000812551656315 6 0.01194416061321495 7 0.002484734142165154 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.282447140550512E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.0897956763882254E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.1795913527764506E-5 64-65 0.0 66-67 2.1795913527764506E-5 68-69 0.0 70-71 0.0 72-73 3.2693870291646763E-4 74-75 2.179591352776451E-4 76-77 3.9232644349976114E-4 78-79 2.615509623331741E-4 80-81 8.936324546383448E-4 82-83 0.0010679997628604608 84-85 0.001242367071082577 86-87 2.1795913527764506E-5 88-89 0.0 90-91 6.538774058329352E-5 92-93 0.0 94-95 0.0 96-97 0.0010897956763882253 98-99 6.102855787774062E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2294008.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.25104221213672 #Duplication Level Percentage of deduplicated Percentage of total 1 94.1487901438158 74.61389741909147 2 4.109494507225594 6.51363445325359 3 0.6489598461232032 1.542922324772752 4 0.2617023110173697 0.8296072358980521 5 0.15358129484113514 0.6085738840224708 6 0.11390937355762665 0.5416461943304111 7 0.09963235084093928 0.5527177349532787 8 0.08415490980594925 0.5335491447511874 9 0.07252872335869454 0.5173179224843059 >10 0.2924585788906258 3.47243769750986 >50 0.005770619898770785 0.31074316185283307 >100 0.007973331189243406 1.431011171562817 >500 2.747393250338062E-4 0.13473366210840484 >1k 5.494786500654898E-4 0.9722684143131365 >5k 5.494786500654899E-5 0.30918196540615706 >10k+ 1.6484359501964694E-4 7.115757613689168 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 133840 5.834330133112004 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18823 0.8205289606662227 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10742 0.4682634062304927 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 7100 0.309501972094256 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4411 0.19228354914193846 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3726 0.1624231476089011 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3125 0.13622445954852816 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2959 0.12898821625731033 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00749779425355099 0.0 0.0 0.0 0.0 2 0.00793371252410628 0.0 0.0 0.0 0.0 3 0.008761957238161332 0.0 0.0 0.0 0.0 4 0.009067100027550035 0.0 0.0 0.0 4.359182705552901E-5 5 0.009154283681661093 0.0 0.0 0.0 4.359182705552901E-5 6 0.009154283681661093 0.0 0.0 0.0 4.359182705552901E-5 7 0.009154283681661093 0.0 0.0 0.0 4.359182705552901E-5 8 0.009197875508716622 0.0 0.0 0.0 8.718365411105802E-5 9 0.009241467335772151 4.359182705552901E-5 0.0 0.0 8.718365411105802E-5 10-11 0.009241467335772151 8.718365411105802E-5 0.0 0.0 8.718365411105802E-5 12-13 0.009241467335772151 1.3077548116658704E-4 0.0 0.0 8.718365411105802E-5 14-15 0.00928505916282768 1.3077548116658704E-4 0.0 0.0 8.718365411105802E-5 16-17 0.00928505916282768 1.3077548116658704E-4 0.0 0.0 1.0897956763882253E-4 18-19 0.00928505916282768 1.9616322174988057E-4 0.0 0.0 1.3077548116658704E-4 20-21 0.00928505916282768 2.1795913527764506E-4 0.0 0.0 1.3077548116658704E-4 22-23 0.00932865098988321 2.1795913527764506E-4 0.0 2.1795913527764506E-5 1.3077548116658704E-4 24-25 0.009372242816938738 2.1795913527764506E-4 0.0 4.359182705552901E-5 1.3077548116658704E-4 26-27 0.009459426471049796 2.1795913527764506E-4 0.0 4.359182705552901E-5 1.9616322174988057E-4 28-29 0.009503018298105325 2.3975504880540955E-4 0.0 4.359182705552901E-5 2.3975504880540955E-4 30-31 0.00956840603868862 2.833468758609386E-4 0.0 1.0897956763882253E-4 2.615509623331741E-4 32-33 0.009699181519855205 3.051427893887031E-4 0.0 1.7436730822211605E-4 2.615509623331741E-4 34-35 0.009917140655132852 3.487346164442321E-4 0.0 1.7436730822211605E-4 2.615509623331741E-4 36-37 0.010244079358049317 3.487346164442321E-4 0.0 1.7436730822211605E-4 3.051427893887031E-4 38-39 0.010919752677410018 3.487346164442321E-4 0.0 1.7436730822211605E-4 3.051427893887031E-4 40-41 0.01190056878615942 3.487346164442321E-4 0.0 1.7436730822211605E-4 3.051427893887031E-4 42-43 0.01320832359782529 3.487346164442321E-4 0.0 1.7436730822211605E-4 3.9232644349976114E-4 44-45 0.014516078409491161 4.359182705552901E-4 0.0 2.1795913527764506E-4 4.359182705552901E-4 46-47 0.016172567837601265 5.013060111385836E-4 0.0 2.1795913527764506E-4 4.795100976108191E-4 48-49 0.01828677144979442 5.231019246663481E-4 0.0 2.1795913527764506E-4 5.231019246663481E-4 50-51 0.020531750543154165 5.448978381941127E-4 0.0 2.1795913527764506E-4 5.231019246663481E-4 52-53 0.023626770264096725 5.884896652496417E-4 0.0 2.1795913527764506E-4 5.231019246663481E-4 54-55 0.028051340710232922 6.538774058329352E-4 0.0 2.1795913527764506E-4 5.666937517218772E-4 56-57 0.03354391091922958 6.756733193606997E-4 0.0 2.1795913527764506E-4 6.102855787774062E-4 58-59 0.03988652175580905 6.974692328884642E-4 0.0 2.1795913527764506E-4 6.320814923051706E-4 60-61 0.048103581155776266 7.410610599439932E-4 0.0 2.1795913527764506E-4 7.192651464162287E-4 62-63 0.057846354502687006 8.064488005272868E-4 0.0 2.615509623331741E-4 7.628569734717578E-4 64-65 0.06911484179654126 8.936324546383448E-4 0.0 3.051427893887031E-4 7.846528869995223E-4 66-67 0.08326038967606042 9.372242816938738E-4 0.0 3.487346164442321E-4 8.282447140550512E-4 68-69 0.10143818155821602 9.590201952216383E-4 0.0 3.487346164442321E-4 8.936324546383448E-4 70-71 0.12552266600639578 0.0010026120222771673 0.0 3.705305299719966E-4 9.372242816938738E-4 72-73 0.15320347618665672 0.0011333875034437544 0.0 3.9232644349976114E-4 9.590201952216383E-4 74-75 0.185199877245415 0.0011333875034437544 0.0 3.9232644349976114E-4 9.590201952216383E-4 76-77 0.22499921534711298 0.0011769793304992834 0.0 3.9232644349976114E-4 0.0010026120222771673 78-79 0.27186042943180666 0.0011769793304992834 0.0 3.9232644349976114E-4 0.0010897956763882253 80-81 0.33073119187029865 0.0012423670710825768 0.0 3.9232644349976114E-4 0.0010897956763882253 82-83 0.40219999232783843 0.0012641629846103413 0.0 3.9232644349976114E-4 0.0011333875034437544 84-85 0.4907131971640901 0.0012859588981381058 0.0 3.9232644349976114E-4 0.0011333875034437544 86-87 0.5967721123901921 0.001416734379304693 0.0 3.9232644349976114E-4 0.0011333875034437544 88 0.6895791121914134 0.0014385302928324574 0.0 3.9232644349976114E-4 0.0011333875034437544 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2625 0.0 88.960144 1 AACGAGT 34755 0.0 77.45866 1 ACGAGTG 38170 0.0 76.802124 2 CGAGTGG 39640 0.0 76.18189 3 GTGGAAT 42065 0.0 76.14181 6 TGGAATC 42190 0.0 75.82213 7 GAATCAC 42225 0.0 75.75433 9 GGAATCA 42410 0.0 75.412796 8 AGTGGAA 41440 0.0 74.593834 5 GAGTGGA 41485 0.0 74.54692 4 ATCGAGT 975 0.0 68.08597 1 AACGTGT 1480 0.0 66.48562 1 CGTGTGG 1735 0.0 62.859127 3 ACGTGTG 1710 0.0 60.48057 2 TCGAGTG 1125 0.0 60.172672 2 GCGAGTG 575 0.0 44.148426 2 GTGTGGA 2710 0.0 42.323486 4 GAGTACT 21835 0.0 41.520653 20-21 AGTACTT 22295 0.0 41.349094 20-21 TGAGTGG 2125 0.0 40.704155 3 >>END_MODULE