FastQCFastQC Report
Thu 26 May 2016
SRR2132777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679204
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1076736.4121452783580795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA378262.25261492945467No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88600.5276309489496214No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG50470.3005590744185936No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35000.2084320904428527No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA29980.17853697347076353No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29940.17829876536740027No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29780.17734593295394724No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28040.16698388045764542No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24990.14882051257619683No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC24210.14417545456061326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT22000.088.804561
AACGAGT278700.079.914541
ACGAGTG307800.078.797292
CGAGTGG319800.077.967493
GTGGAAT339900.077.6235666
TGGAATC344200.076.587817
GAATCAC344650.076.542369
GGAATCA345100.076.374458
GAGTGGA336200.076.007654
ATCGAGT6450.075.907281
AGTGGAA338150.075.638855
CGTGTGG10850.070.623043
AACGTGT10100.069.916511
ACGTGTG11250.065.608712
TCGAGTG7900.063.6753162
GCGAGTG4650.045.496082
TGAGTGG17100.044.5356143
ATGAGTG16050.044.522852
TCCGATC2350.044.0052576
GTGTGGA19400.042.8891224
Produced by FastQC (version 0.11.5)