##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132777_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1679204
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.822768406935666	31.0	25.0	33.0	16.0	34.0
2	28.137122112620027	31.0	25.0	34.0	16.0	34.0
3	29.22912880150357	31.0	28.0	34.0	16.0	34.0
4	34.7158582280652	35.0	35.0	37.0	32.0	37.0
5	33.854540008242	35.0	35.0	37.0	28.0	37.0
6	35.24327240764077	37.0	35.0	37.0	32.0	37.0
7	35.64154206397793	37.0	35.0	37.0	33.0	37.0
8	35.907394217736496	37.0	35.0	37.0	35.0	37.0
9	37.87131402736058	39.0	38.0	39.0	35.0	39.0
10-11	37.87037697623398	39.0	38.0	39.0	35.0	39.0
12-13	37.85020462076079	39.0	38.0	39.0	35.0	39.0
14-15	39.27129163579887	41.0	39.0	41.0	36.0	41.0
16-17	39.248940271700164	40.5	39.0	41.0	36.0	41.0
18-19	39.130798878516245	40.5	39.0	41.0	35.5	41.0
20-21	39.07620426106655	40.0	39.0	41.0	35.0	41.0
22-23	38.94700286564348	40.0	39.0	41.0	35.0	41.0
24-25	38.83800479274704	40.0	39.0	41.0	35.0	41.0
26-27	38.65562314048799	40.0	39.0	41.0	35.0	41.0
28-29	38.48472401209145	40.0	38.0	41.0	35.0	41.0
30-31	38.36418773418834	40.0	38.0	41.0	34.5	41.0
32-33	38.21443404136722	40.0	38.0	41.0	34.0	41.0
34-35	38.132875755417444	40.0	38.0	41.0	34.0	41.0
36-37	37.99715519972558	40.0	38.0	41.0	34.0	41.0
38-39	37.56261091564813	40.0	36.0	41.0	33.5	41.0
40-41	37.33186706320376	40.0	35.0	41.0	33.0	41.0
42-43	37.19453532745277	40.0	35.0	41.0	33.0	41.0
44-45	37.067513238415344	40.0	35.0	41.0	33.0	41.0
46-47	36.947543300277985	40.0	35.0	41.0	33.0	41.0
48-49	36.80678613200064	40.0	35.0	41.0	32.5	41.0
50-51	36.553012915643365	39.5	35.0	41.0	31.5	41.0
52-53	36.160509681968364	39.0	35.0	41.0	30.5	41.0
54-55	35.393259246643055	39.0	35.0	41.0	27.0	41.0
56-57	34.73246728807221	39.0	35.0	41.0	21.5	41.0
58-59	34.40896490241805	39.0	35.0	41.0	18.5	41.0
60-61	34.178751956284046	39.0	35.0	41.0	17.5	41.0
62-63	33.9212442323863	38.0	35.0	40.0	14.0	41.0
64-65	33.67362899326109	37.5	34.0	40.0	12.0	41.0
66-67	33.38786978830446	37.0	34.0	40.0	9.0	41.0
68-69	33.07946354344082	36.5	34.0	40.0	7.0	41.0
70-71	32.75221295328025	36.0	34.0	39.0	3.5	41.0
72-73	32.42480603905184	35.5	34.0	39.0	2.0	40.5
74-75	32.09188818035212	35.0	33.0	38.5	2.0	40.0
76-77	31.758486163682317	35.0	33.0	37.0	2.0	39.5
78-79	31.422985533621883	35.0	33.0	37.0	2.0	39.0
80-81	31.112796301104574	35.0	33.0	36.5	2.0	39.0
82-83	30.819641925579024	35.0	33.0	36.0	2.0	37.5
84-85	30.54180284229909	35.0	33.0	36.0	2.0	37.0
86-87	30.323462188036714	35.0	33.0	35.0	2.0	37.0
88-89	30.10997978804243	35.0	32.0	35.0	2.0	36.0
90-91	29.91925311040231	35.0	32.0	35.0	2.0	36.0
92-93	29.74904240342448	35.0	32.0	35.0	2.0	36.0
94-95	29.57456330499451	35.0	32.0	35.0	2.0	36.0
96-97	29.41354445320521	35.0	32.0	35.0	2.0	35.0
98-99	29.25394621499234	35.0	32.0	35.0	2.0	35.0
100	29.1536084954538	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.04202814957440992
1101	2	2.0614052857222553
1101	3	1.0145212228632658
1101	4	0.6178708106071937
1101	5	2.187716843235947
1101	6	0.9918197944657337
1101	7	0.5412484750036128
1101	8	0.34244123623792433
1101	9	0.3582481857099111
1101	10-11	0.436779290662038
1101	12-13	0.3331260856121361
1101	14-15	0.4373387251349996
1101	16-17	0.42843863309884256
1101	18-19	0.4029619340661128
1101	20-21	0.3864109537412759
1101	22-23	0.36534265786732334
1101	24-25	0.2841349810940841
1101	26-27	0.29025805346195455
1101	28-29	0.3093707920199762
1101	30-31	0.3489286374963996
1101	32-33	0.4128881007846843
1101	34-35	0.32372785740091103
1101	36-37	0.34851329290445676
1101	38-39	0.35477099594472605
1101	40-41	0.38774419062023924
1101	42-43	0.3465766424554815
1101	44-45	0.47941819069884417
1101	46-47	0.48537038683675604
1101	48-49	0.48178986884686736
1101	50-51	0.3471007571769391
1101	52-53	0.4080263282956409
1101	54-55	0.43578811284236707
1101	56-57	0.4858965088182501
1101	58-59	0.48991654381769223
1101	60-61	0.5934904791509084
1101	62-63	0.4465183215528157
1101	64-65	0.479834647945907
1101	66-67	0.33936648994314567
1101	68-69	0.4544397918957088
1101	70-71	0.47522270165285363
1101	72-73	0.4875015455035481
1101	74-75	0.4841955587494198
1101	76-77	0.453081203597538
1101	78-79	0.4555171487147227
1101	80-81	0.5004139185494267
1101	82-83	0.5226655443791266
1101	84-85	0.5911199531375928
1101	86-87	0.5550990607427195
1101	88-89	0.5078658107453435
1101	90-91	0.5340108499180225
1101	92-93	0.5528103411699306
1101	94-95	0.6370705815890112
1101	96-97	0.5836216521957489
1101	98-99	0.5740130351259793
1101	100	0.6778061881000035
1102	1	1.7876386064052383
1102	2	3.0818602983060224
1102	3	3.2433240480135446
1102	4	1.4127014741064343
1102	5	2.6201874547802504
1102	6	1.1816648695958918
1102	7	0.7254561720144608
1102	8	0.5100705200271278
1102	9	0.3994248497751869
1102	10-11	0.48111824525295077
1102	12-13	0.40374970732239746
1102	14-15	0.588361124752474
1102	16-17	0.4828132725805574
1102	18-19	0.4244863623362889
1102	20-21	0.34269927008239165
1102	22-23	0.356449061088
1102	24-25	0.31977892053234314
1102	26-27	0.35928964204606473
1102	28-29	0.3594195371466071
1102	30-31	0.3076274292750796
1102	32-33	0.3151324436784009
1102	34-35	0.3416301698010784
1102	36-37	0.28971350075539704
1102	38-39	0.2826679898674129
1102	40-41	0.37043976817165714
1102	42-43	0.4075377567278622
1102	44-45	0.42837361234126803
1102	46-47	0.4459398760406046
1102	48-49	0.37966652099074594
1102	50-51	0.5170651138875257
1102	52-53	0.5693845278187624
1102	54-55	0.5252563183963943
1102	56-57	0.47661088395695117
1102	58-59	0.36640117710990694
1102	60-61	0.3155568695063522
1102	62-63	0.3518626828941507
1102	64-65	0.4783017667936562
1102	66-67	0.4086764828176115
1102	68-69	0.4272051830768149
1102	70-71	0.3800003090816162
1102	72-73	0.3434875497565031
1102	74-75	0.30565572587519796
1102	76-77	0.24597155234847534
1102	78-79	0.21916958679658194
1102	80-81	0.2449828248138317
1102	82-83	0.26006919653899985
1102	84-85	0.29449412473370273
1102	86-87	0.25497774069427237
1102	88-89	0.3091032830397964
1102	90-91	0.40237203213067474
1102	92-93	0.38577946734967483
1102	94-95	0.3469876765224704
1102	96-97	0.3603148254469417
1102	98-99	0.3758759582917257
1102	100	0.33914267689863564
1103	1	1.500128304899846
1103	2	3.1261429439588753
1103	3	3.3005238637806364
1103	4	1.4788935801489558
1103	5	2.616398210381533
1103	6	1.2131493875457764
1103	7	0.7522257556853091
1103	8	0.4842418365130001
1103	9	0.4079134410622416
1103	10-11	0.43219809945554033
1103	12-13	0.4181427770679207
1103	14-15	0.5778713136264031
1103	16-17	0.49242836710803317
1103	18-19	0.43192550740310054
1103	20-21	0.387468393170991
1103	22-23	0.4273985267673339
1103	24-25	0.479637417193878
1103	26-27	0.5592044644981371
1103	28-29	0.5403780812239773
1103	30-31	0.5304422028028668
1103	32-33	0.5889881909253418
1103	34-35	0.5518299674666878
1103	36-37	0.4949052206328872
1103	38-39	0.5155265740194608
1103	40-41	0.5662414050488067
1103	42-43	0.6082851846826927
1103	44-45	0.5873899499380073
1103	46-47	0.6419604906162277
1103	48-49	0.6407438761826256
1103	50-51	0.7742655456079603
1103	52-53	0.8330533495678978
1103	54-55	0.7513700877442417
1103	56-57	0.8169144835882776
1103	58-59	0.7829916038373597
1103	60-61	0.7691435281077972
1103	62-63	0.7998041919647534
1103	64-65	0.8131422373920714
1103	66-67	0.8357561549997001
1103	68-69	0.8836705436207026
1103	70-71	0.8686042294612264
1103	72-73	0.8640415356785809
1103	74-75	0.8991354449200202
1103	76-77	0.9084545130677419
1103	78-79	0.8907789019218306
1103	80-81	0.8312843618814014
1103	82-83	0.7606171436042217
1103	84-85	0.8128179824419419
1103	86-87	0.872580895280457
1103	88-89	0.8729300653950709
1103	90-91	0.8963984160521257
1103	92-93	0.9401589710370963
1103	94-95	0.8149367329070891
1103	96-97	0.8687831154146473
1103	98-99	0.8178842025129498
1103	100	0.8670200966524853
1104	1	6.155671369186546
1104	2	5.133280556953874
1104	3	4.180762931371152
1104	4	1.8908331937863636
1104	5	2.9265895562265243
1104	6	1.3297923462939991
1104	7	0.7600906664364686
1104	8	0.5713730254268725
1104	9	0.47297102836090943
1104	10-11	0.49735434228581
1104	12-13	0.44442874045065395
1104	14-15	0.5759211461414253
1104	16-17	0.46609766458887236
1104	18-19	0.39784919849657285
1104	20-21	0.3570939610138737
1104	22-23	0.37910664405269756
1104	24-25	0.43207168507805704
1104	26-27	0.45311535331565267
1104	28-29	0.47856006626503955
1104	30-31	0.6191644989530047
1104	32-33	0.5950474760168944
1104	34-35	0.6909262369110039
1104	36-37	0.6779009073212734
1104	38-39	0.7970171902723848
1104	40-41	0.7963175762964596
1104	42-43	0.7350678671954185
1104	44-45	0.7247012425549002
1104	46-47	0.7238514647822498
1104	48-49	0.7593760057655246
1104	50-51	0.8420981065220161
1104	52-53	0.961361751348285
1104	54-55	1.1041037636952282
1104	56-57	1.2207173101839714
1104	58-59	1.2760593320069518
1104	60-61	1.164226811730984
1104	62-63	1.176941346096818
1104	64-65	1.116766107442082
1104	66-67	1.1362012520492115
1104	68-69	1.131033228886615
1104	70-71	1.1947972730990912
1104	72-73	1.186131197496593
1104	74-75	1.2677882233351028
1104	76-77	1.2527898501025767
1104	78-79	1.1907548116176052
1104	80-81	1.239093061922695
1104	82-83	1.1951488739763576
1104	84-85	1.1877709489908348
1104	86-87	1.163542442566282
1104	88-89	1.0983315704655041
1104	90-91	1.0590492672428802
1104	92-93	1.0867450307810493
1104	94-95	1.0972475666610464
1104	96-97	1.0966418315161448
1104	98-99	1.1662873594899636
1104	100	1.1179424111571414
1105	1	1.6654703735680023
1105	2	3.09905977970109
1105	3	3.3209108895330637
1105	4	1.5011796873419456
1105	5	2.6414839596903334
1105	6	1.2358771242171045
1105	7	0.7777172863073645
1105	8	0.4962478600005227
1105	9	0.41399468395795225
1105	10-11	0.46934688079219455
1105	12-13	0.39772214366071523
1105	14-15	0.598252738103028
1105	16-17	0.46618632776566926
1105	18-19	0.4205079290561926
1105	20-21	0.3439152375615748
1105	22-23	0.38902586666917927
1105	24-25	0.4342939693671468
1105	26-27	0.49004190252935587
1105	28-29	0.637684440140788
1105	30-31	0.6449095637953874
1105	32-33	0.6478898379991662
1105	34-35	0.611786210300167
1105	36-37	0.6773275085324855
1105	38-39	0.6416448376140025
1105	40-41	0.679697984705669
1105	42-43	0.6611861849958629
1105	44-45	0.7474905314251146
1105	46-47	0.7810532382834836
1105	48-49	0.7147323729047628
1105	50-51	0.6876691371957975
1105	52-53	0.8016164381063859
1105	54-55	0.7817822146805966
1105	56-57	0.6960688876100392
1105	58-59	0.6088981641013547
1105	60-61	0.6240952550682621
1105	62-63	0.6320697561675743
1105	64-65	0.5531746426396467
1105	66-67	0.6038103116193412
1105	68-69	0.5892682968369556
1105	70-71	0.5527706301710182
1105	72-73	0.543958248441669
1105	74-75	0.5794884348312479
1105	76-77	0.5394559066651645
1105	78-79	0.5649594726146603
1105	80-81	0.5678628960001397
1105	82-83	0.6101249283817936
1105	84-85	0.6192126229421042
1105	86-87	0.6945461489485467
1105	88-89	0.5526544054527562
1105	90-91	0.6052965032039985
1105	92-93	0.7132800417421237
1105	94-95	0.713282290298757
1105	96-97	0.7231849838010938
1105	98-99	0.6165652638775398
1105	100	0.7195508277409601
1106	1	-0.317261330397983
1106	2	2.092572482288535
1106	3	2.8685124230306123
1106	4	1.296424529058804
1106	5	2.4596443044732936
1106	6	1.1342698496584944
1106	7	0.6158593489046211
1106	8	0.43461962309454805
1106	9	0.36528637431994326
1106	10-11	0.43796861008183186
1106	12-13	0.35406749893815714
1106	14-15	0.4139907779421321
1106	16-17	0.3274523437271455
1106	18-19	0.3547939574557404
1106	20-21	0.40077276253872185
1106	22-23	0.36051135352126806
1106	24-25	0.44150264873684364
1106	26-27	0.426773457489972
1106	28-29	0.36440749018409235
1106	30-31	0.4044284312384079
1106	32-33	0.4545923103031555
1106	34-35	0.49467868768145706
1106	36-37	0.45443681147517623
1106	38-39	0.4325136744214291
1106	40-41	0.4573412034250026
1106	42-43	0.530579041300733
1106	44-45	0.5970490425227482
1106	46-47	0.6323875378941111
1106	48-49	0.5763468945430716
1106	50-51	0.7463204716284437
1106	52-53	0.790767770241068
1106	54-55	0.7822367899318223
1106	56-57	0.8358695542155914
1106	58-59	0.8832636878371147
1106	60-61	0.8509024230009459
1106	62-63	0.9241471451095862
1106	64-65	0.8888033884860604
1106	66-67	0.8412627163750273
1106	68-69	0.7513293629172111
1106	70-71	0.8433519785197063
1106	72-73	0.783010344446609
1106	74-75	0.8374715914182502
1106	76-77	0.8535341451313379
1106	78-79	0.8129894449494905
1106	80-81	0.7694107498268323
1106	82-83	0.8243166504074289
1106	84-85	0.8265536655298256
1106	86-87	0.8606526747309502
1106	88-89	0.8197183633500273
1106	90-91	0.8158209029554264
1106	92-93	0.8621405860132931
1106	94-95	0.8698396516844475
1106	96-97	0.9407991126070492
1106	98-99	0.8394361258034984
1106	100	0.869805818030212
1107	1	-0.8606040245257667
1107	2	1.9048278902481783
1107	3	-0.6293652812627712
1107	4	0.015088213984249421
1107	5	1.896017575663329
1107	6	0.8129139433901855
1107	7	0.3533931662901111
1107	8	0.2733108510811135
1107	9	0.2316736804304611
1107	10-11	0.31668576316280195
1107	12-13	0.28056245627525556
1107	14-15	0.34163743909851263
1107	16-17	0.33389229019627464
1107	18-19	0.2608373139464959
1107	20-21	0.20143562719266583
1107	22-23	0.18934609139184744
1107	24-25	0.10591396597496328
1107	26-27	0.13062252502690797
1107	28-29	0.13115651846339205
1107	30-31	0.13446805260911532
1107	32-33	0.17726748655491775
1107	34-35	0.23340033093359835
1107	36-37	0.2791537613696278
1107	38-39	0.29424217709985356
1107	40-41	0.26267785965222856
1107	42-43	0.29907465836689795
1107	44-45	0.3937604311269993
1107	46-47	0.40952560564050344
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2214	100	-1.9224047650581682
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2215	100	-0.7568213663961743
2216	1	2.9101602853685584
2216	2	-2.072651483167018
2216	3	-2.2492163579640625
2216	4	-0.9096141324908942
2216	5	-2.517294777118849
2216	6	-1.0623315805443738
2216	7	-0.47355332686868934
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2216	9	-0.3083592706192064
2216	10-11	-0.12682024139370895
2216	12-13	-0.206768444343858
2216	14-15	-0.1922599878878799
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2216	26-27	-0.31151877834156494
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2216	30-31	-0.31875979875075444
2216	32-33	-0.25693177719507077
2216	34-35	-0.39498358740958395
2216	36-37	-0.4535073576789017
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2216	54-55	0.08450720022065639
2216	56-57	0.2504720332992463
2216	58-59	0.30499255215408994
2216	60-61	0.24683286357988976
2216	62-63	0.2739945917710571
2216	64-65	0.14792301481002568
2216	66-67	0.2085311416324771
2216	68-69	0.18696165668225007
2216	70-71	0.08098253722027238
2216	72-73	0.14756932438554315
2216	74-75	0.12377806066005803
2216	76-77	0.1681997286080339
2216	78-79	0.2465911251192736
2216	80-81	0.2225510204772867
2216	82-83	0.22914235974981878
2216	84-85	0.20731724093896275
2216	86-87	0.2169886008583788
2216	88-89	0.1548235210007327
2216	90-91	0.19704912274986697
2216	92-93	0.2005841498553309
2216	94-95	0.15598548042280314
2216	96-97	0.22681076792718002
2216	98-99	0.21160488287976875
2216	100	0.14478560011787778
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	849.0
3	213.0
4	237.0
5	562.0
6	1527.0
7	2684.0
8	3259.0
9	4478.0
10	3391.0
11	3462.0
12	3081.0
13	4187.0
14	4226.0
15	4458.0
16	5404.0
17	6267.0
18	9106.0
19	16282.0
20	32976.0
21	38787.0
22	17757.0
23	10890.0
24	10238.0
25	10583.0
26	11457.0
27	12792.0
28	15388.0
29	18223.0
30	21782.0
31	26770.0
32	34734.0
33	48540.0
34	70970.0
35	137814.0
36	195923.0
37	340706.0
38	420530.0
39	128120.0
40	551.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.642072419464684	41.21825431376361	25.75013030446518	19.38954296230653
2	14.982964158924952	40.60301100525458	25.516539446265185	18.897485389555282
3	18.36178649232283	26.317196840752437	19.667340426672318	35.653676240252416
4	31.422090466673495	27.561928151671864	22.09362293086486	18.92235845078978
5	17.28470128343399	39.03131731388242	22.370556353239436	21.31342504944416
6	35.15927914320531	24.276028259644715	24.120042739675114	16.444649857474857
7	18.97171082173327	23.65244962058365	39.03581437839679	18.340025179286286
8	34.33436318636687	23.99476180380704	23.835638790760385	17.8352362190657
9	33.80452881246114	25.83295418543548	23.636973232555427	16.72554376954795
10-11	16.810554286435718	42.24671332369385	23.698579803287746	17.24415258658269
12-13	16.679390949521324	25.554935552797637	31.866348579445976	25.899324918235067
14-15	16.894433314832504	33.57111464717807	23.702093372812357	25.832358665177075
16-17	25.484693938318394	25.153793106733907	31.918724586172974	17.44278836877473
18-19	17.16378712771051	25.230674772094396	32.09520701475223	25.510331085442868
20-21	25.565416709345616	33.763676122734346	23.630005645532048	17.040901522387987
22-23	25.20661575365471	25.459830967529857	32.12414929931086	17.209403979504575
24-25	17.02407807508796	33.66872041753116	23.540588278732066	25.76661322864881
26-27	17.442401280606763	28.81475389529801	36.45837551601831	17.284469308076922
28-29	24.83390939992997	25.251547757151606	32.64490198927587	17.26964085364256
30-31	22.917138120204573	26.143220240066128	33.01546447007034	17.924177169658957
32-33	19.45052536797197	27.82142610427322	33.92419860838826	18.80384991936656
34-35	19.16491385203942	27.5163112998778	34.14281409524989	19.17596075283289
36-37	19.068231138086855	27.708902551446997	34.35955369329754	18.863312617168614
38-39	19.590591732749566	27.256307155056803	34.09436852222839	19.058732589965246
40-41	19.06266302367074	28.16280809240569	33.88507292741084	18.88945595651273
42-43	18.971334920610346	27.638867880032347	34.286434022198854	19.103363177158457
44-45	19.121530201214384	27.979179420725536	33.782077698719156	19.117212679340927
46-47	19.451180440256216	27.507884688221324	33.963741153546565	19.0771937179759
48-49	19.14177789000026	27.902893275623452	33.79101050259528	19.16431833178101
50-51	19.312126459917913	28.183472645372447	33.37771944326002	19.126681451449613
52-53	19.459749333314278	28.070952750175383	33.074222160288045	19.3950757562223
54-55	19.959635636885096	28.851914359422683	31.45588624133816	19.73256376235407
56-57	20.25745531811501	29.397917108344192	30.18388474539127	20.160742828149527
58-59	20.696740836729784	29.969795212493537	29.20428369632278	20.129180254453896
60-61	20.589428086164634	29.990578869511985	29.109179111055	20.31081393326838
62-63	19.909617863469197	30.230582535112628	28.92838777432277	20.931411827095406
64-65	20.24203134342224	30.175577237786477	29.396785619853215	20.18560579893807
66-67	20.345681644398177	29.81564479360459	29.4571713740558	20.38150218794143
68-69	20.447664488650574	29.960802856591574	29.31088182257784	20.280650832180008
70-71	19.617687904507136	30.362064406706992	29.54676739693331	20.47348029185257
72-73	19.670544713065063	30.127156091367535	29.54583622162035	20.656462973947058
74-75	19.97007499117882	30.15050343988721	29.19302226212223	20.686399306811737
76-77	20.418462791810473	29.8271759627464	29.335064720057574	20.41929652538555
78-79	20.238852403173897	30.17107591966632	28.878254831478873	20.711816845680907
80-81	20.243051564711816	30.38641822163173	28.836654079256025	20.533876134400433
82-83	20.285971212310695	30.543506095200662	28.624217628737664	20.546305063750975
84-85	20.368928974472738	30.714959935921293	28.26174602560171	20.654365064004267
86-87	20.246503773961884	30.97805001001963	28.215341380600982	20.560104835417505
88-89	20.868727087358057	30.469555813349658	28.118799145309325	20.542917953982958
90-91	20.48278870475717	30.997720644174837	28.019699827745608	20.499790823322382
92-93	20.150202119575702	31.097740357931496	28.01756070137994	20.73449682111286
94-95	20.200553357424113	31.33690129370821	27.822021624531622	20.640523724336056
96-97	20.108058534435656	30.781615458712796	28.091377798422744	21.018948208428803
98-99	20.57033280828016	30.91651058989575	27.455030535464907	21.05812606635918
100	20.358455553941035	31.28446573495537	27.24808897549077	21.108989735612827
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41434.0
1	22335.5
2	2277.0
3	1124.0
4	868.0
5	759.0
6	788.5
7	896.5
8	849.0
9	723.5
10	680.5
11	1182.5
12	8984.0
13	10155.0
14	3729.5
15	3695.5
16	4290.0
17	4966.0
18	5757.0
19	6721.5
20	7762.5
21	8867.5
22	10220.5
23	12043.0
24	14064.5
25	16266.0
26	19135.5
27	22168.0
28	24317.5
29	26182.0
30	28343.0
31	30821.0
32	33702.0
33	36824.5
34	40153.0
35	43136.5
36	45644.5
37	47936.0
38	49699.0
39	50783.5
40	51761.0
41	53059.5
42	53761.0
43	54340.5
44	54923.0
45	55004.5
46	55461.0
47	55944.0
48	55451.0
49	54924.0
50	58458.5
51	57079.5
52	48544.5
53	43087.5
54	39949.0
55	38066.5
56	34271.5
57	28792.0
58	25875.0
59	21605.0
60	16369.5
61	13134.5
62	10153.0
63	7555.0
64	5517.0
65	4063.5
66	3091.5
67	2389.0
68	1839.5
69	1410.0
70	1064.5
71	773.0
72	596.5
73	433.0
74	311.0
75	229.0
76	142.0
77	75.0
78	53.0
79	34.0
80	16.5
81	9.5
82	6.0
83	4.0
84	2.5
85	1.0
86	0.5
87	1.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14066188503600516
2	0.00577654650655906
3	0.004168641808857054
4	0.0
5	0.002203424956110157
6	0.01202950921984464
7	0.002739393188677492
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.001071936465134671
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.191040516816301E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.9776012920407526E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.764162067265204E-4
74-75	3.870881679652978E-4
76-77	6.25296271328558E-4
78-79	4.764162067265204E-4
80-81	7.741763359305956E-4
82-83	8.337283617714107E-4
84-85	0.001131488490975486
86-87	2.9776012920407526E-5
88-89	0.0
90-91	8.932803876122258E-5
92-93	0.0
94-95	0.0
96-97	0.0012803685555775237
98-99	5.359682325673355E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1679204.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.31230047770315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.79075813807873	69.69807000788488
2	4.364115512910897	6.486149266296803
3	0.6515447971545667	1.452533782225029
4	0.2762561661953369	0.8211692492450469
5	0.13500345854015877	0.5016208788282709
6	0.09571242071473171	0.4267566100560889
7	0.08271059133227202	0.43024900210406153
8	0.058017898596254225	0.34491548108558046
9	0.054300865655821894	0.3631700020333345
>10	0.46435144756847413	5.867797284727104
>50	0.013761586919159291	0.6892931681771192
>100	0.011863997222726423	1.732604675772648
>500	5.610916888335413E-4	0.34006077847369076
>1k	7.214035999288389E-4	1.3504743334306386
>5k	1.6031191109529752E-4	0.8283806702108278
>10k+	1.6031191109529752E-4	8.666754809448857
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	107673	6.4121452783580795	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37826	2.25261492945467	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8860	0.5276309489496214	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	5047	0.3005590744185936	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3500	0.2084320904428527	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	2998	0.17853697347076353	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2994	0.17829876536740027	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2978	0.17734593295394724	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2804	0.16698388045764542	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2499	0.14882051257619683	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	2421	0.14417545456061326	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.955202584081506E-4	0.0	0.0	1.191040516816301E-4	0.0
2	5.955202584081506E-4	0.0	0.0	1.191040516816301E-4	0.0
3	5.955202584081506E-4	0.0	0.0	1.7865607752244516E-4	0.0
4	5.955202584081506E-4	0.0	0.0	1.7865607752244516E-4	0.0
5	5.955202584081506E-4	5.955202584081505E-5	0.0	1.7865607752244516E-4	0.0
6	5.955202584081506E-4	5.955202584081505E-5	0.0	2.382081033632602E-4	0.0
7	5.955202584081506E-4	5.955202584081505E-5	0.0	2.382081033632602E-4	0.0
8	5.955202584081506E-4	1.191040516816301E-4	0.0	2.977601292040753E-4	0.0
9	5.955202584081506E-4	1.191040516816301E-4	0.0	2.977601292040753E-4	0.0
10-11	5.955202584081506E-4	1.191040516816301E-4	0.0	2.977601292040753E-4	0.0
12-13	6.25296271328558E-4	1.191040516816301E-4	0.0	2.977601292040753E-4	0.0
14-15	6.550722842489655E-4	1.191040516816301E-4	0.0	2.977601292040753E-4	0.0
16-17	6.550722842489655E-4	1.191040516816301E-4	0.0	2.977601292040753E-4	0.0
18-19	7.741763359305957E-4	2.0843209044285268E-4	0.0	3.573121550448903E-4	0.0
20-21	8.932803876122258E-4	3.2753614212448277E-4	0.0	4.1686418088570537E-4	0.0
22-23	9.528324134530408E-4	3.573121550448903E-4	0.0	5.06192219646928E-4	0.0
24-25	9.528324134530408E-4	3.573121550448903E-4	0.0	5.359682325673355E-4	0.0
26-27	0.001012384439293856	4.4664019380611286E-4	0.0	7.444003230101881E-4	0.0
28-29	0.0011017124780550785	4.764162067265204E-4	0.0	9.528324134530408E-4	0.0
30-31	0.0013399205814183387	4.764162067265204E-4	0.0	9.528324134530408E-4	0.0
32-33	0.0014590246330999689	4.764162067265204E-4	0.0	9.528324134530408E-4	0.0
34-35	0.0015185766589407837	4.764162067265204E-4	0.0	0.0010421604522142633	0.0
36-37	0.001875888813985674	4.764162067265204E-4	0.0	0.001250592542657116	0.0
38-39	0.00265006514991627	4.764162067265204E-4	0.0	0.0012803685555775237	0.0
40-41	0.0036028975633693103	4.764162067265204E-4	0.0	0.001310144568497931	0.0
42-43	0.004913042131867242	4.764162067265204E-4	0.0	0.001310144568497931	0.0
44-45	0.00589565055824069	4.764162067265204E-4	0.0	0.001310144568497931	0.0
46-47	0.007176019113818214	5.65744245487743E-4	0.0	0.001310144568497931	0.0
48-49	0.008843475837361035	5.955202584081506E-4	0.0	0.0013399205814183387	0.0
50-51	0.01080869269010793	5.955202584081506E-4	0.0	0.0013994726072591537	0.0
52-53	0.013607637904626239	6.550722842489655E-4	0.0	0.0014292486201795613	0.0
54-55	0.018252695920209814	6.550722842489655E-4	0.0	0.0015781286847815989	0.0
56-57	0.02370170628464439	6.550722842489655E-4	0.0	0.001637680710622414	0.0
58-59	0.030043997036691195	6.848482971693731E-4	0.0	0.0017865607752244517	0.0
60-61	0.03823240058980326	7.146243100897806E-4	0.0	0.0017865607752244517	0.0
62-63	0.048386020995662235	8.039523488510031E-4	0.0	0.0017865607752244517	0.0
64-65	0.05922448969869057	8.337283617714107E-4	0.0	0.0017865607752244517	0.0
66-67	0.07461868837854127	8.337283617714107E-4	0.0	0.0018163367881448593	0.0
68-69	0.09322869645379597	8.337283617714107E-4	0.0	0.0018461128010652667	0.0
70-71	0.12020576415968519	8.337283617714107E-4	0.0	0.0019056648269060816	0.0
72-73	0.1487311845374356	8.337283617714107E-4	0.0	0.0019652168527468966	0.0
74-75	0.1868147050626368	8.337283617714107E-4	0.0	0.002024768878587712	0.0
76-77	0.2325804369213032	8.337283617714107E-4	0.0	0.0021736489431897496	0.0
78-79	0.28814247703078366	8.337283617714107E-4	0.0	0.0022332009690305644	0.0
80-81	0.3567166347864822	8.337283617714107E-4	0.0	0.0024118570465530096	0.0
82-83	0.4429777442169028	8.337283617714107E-4	0.0	0.002441633059473417	0.0
84-85	0.5504691508595739	8.337283617714107E-4	0.0	0.0024714090723938244	0.0
86-87	0.669573202541204	8.337283617714107E-4	0.0	0.00253096109823464	0.0
88	0.7762606568350242	8.337283617714107E-4	0.0	0.0025607371111550474	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2200	0.0	88.80456	1
AACGAGT	27870	0.0	79.91454	1
ACGAGTG	30780	0.0	78.79729	2
CGAGTGG	31980	0.0	77.96749	3
GTGGAAT	33990	0.0	77.623566	6
TGGAATC	34420	0.0	76.58781	7
GAATCAC	34465	0.0	76.54236	9
GGAATCA	34510	0.0	76.37445	8
GAGTGGA	33620	0.0	76.00765	4
ATCGAGT	645	0.0	75.90728	1
AGTGGAA	33815	0.0	75.63885	5
CGTGTGG	1085	0.0	70.62304	3
AACGTGT	1010	0.0	69.91651	1
ACGTGTG	1125	0.0	65.60871	2
TCGAGTG	790	0.0	63.675316	2
GCGAGTG	465	0.0	45.49608	2
TGAGTGG	1710	0.0	44.535614	3
ATGAGTG	1605	0.0	44.52285	2
TCCGATC	235	0.0	44.005257	6
GTGTGGA	1940	0.0	42.889122	4
>>END_MODULE