FastQCFastQC Report
Thu 26 May 2016
SRR2132776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245156
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4243629.996381758408878No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2930096.90219629149082No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG521121.2275638398211985No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329320.7757547661381584No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236320.5566815448007093No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC179490.42281131718127674No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA117270.2762442652284156No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108060.2545489494379005No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99610.23464390943465918No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98100.23108691412047047No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93590.22046304069862216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87580.206305728222944No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85360.20107623842327585No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82740.19490449820925307No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65830.15507086194241154No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59580.1403481992181206No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA52310.12322279793722539No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48430.11408296891798558No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA47700.1123633619117884No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC43960.1035533205375727No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG43860.10331775793398405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT61600.083.375781
AACGTGT25800.076.269771
ACGTGTG29800.070.367432
ATCGAGT22200.067.644671
CGTGTGG32550.067.019263
TCGAGTG23500.066.223662
GTGGAAT1267450.057.4617166
TGGAATC1278700.056.983857
GGAATCA1288200.056.6514938
GAATCAC1292200.056.497959
AGTGGAA1293650.054.6239625
ATGAGTG30850.054.560832
ACGAGTG1233900.054.378542
CGAGTGG1272800.054.343073
GAGTGGA1307750.054.0278324
AACGAGT1148600.053.858651
AATGAGT29250.052.3062171
TGAGTGG35400.051.928543
AGCGAGT12650.050.2387851
GTGTGGA50100.045.7924924
Produced by FastQC (version 0.11.5)