##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132776_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4245156
Sequences flagged as poor quality	0
Sequence length	100
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.110097720790474	31.0	25.0	33.0	16.0	34.0
2	28.403426634969364	31.0	26.0	34.0	16.0	34.0
3	29.45249903654895	31.0	28.0	34.0	16.0	34.0
4	34.869642717487885	35.0	35.0	37.0	32.0	37.0
5	34.0144343340975	37.0	35.0	37.0	28.0	37.0
6	35.33410739204873	37.0	35.0	37.0	32.0	37.0
7	35.70303776822336	37.0	35.0	37.0	35.0	37.0
8	35.95178221954623	37.0	35.0	37.0	35.0	37.0
9	37.91150478333423	39.0	38.0	39.0	35.0	39.0
10-11	37.89689448397185	39.0	38.0	39.0	35.0	39.0
12-13	37.83575421021041	39.0	38.0	39.0	35.0	39.0
14-15	39.215957905904986	41.0	39.0	41.0	36.0	41.0
16-17	38.987603517986145	40.5	39.0	41.0	36.0	41.0
18-19	38.34682800820512	40.0	37.0	41.0	34.5	41.0
20-21	38.02808035794209	40.0	37.0	41.0	34.0	41.0
22-23	37.73871231116124	40.0	36.5	41.0	34.0	41.0
24-25	37.33019823064217	40.0	36.0	41.0	33.0	41.0
26-27	36.98871890691414	40.0	35.5	41.0	33.0	41.0
28-29	36.8371581633278	40.0	35.0	41.0	33.0	41.0
30-31	36.70376047429117	40.0	35.0	41.0	32.5	41.0
32-33	36.55389095241729	40.0	35.0	41.0	31.5	41.0
34-35	36.4360658359787	40.0	35.0	41.0	31.0	41.0
36-37	36.22030297590948	39.5	35.0	41.0	31.0	41.0
38-39	35.73815108325819	38.5	35.0	41.0	30.0	41.0
40-41	35.48955951206504	38.0	35.0	41.0	30.0	41.0
42-43	35.31192728842002	38.0	35.0	41.0	29.5	41.0
44-45	35.18143985756943	38.0	35.0	41.0	29.0	41.0
46-47	35.029660865230866	38.0	35.0	40.5	28.5	41.0
48-49	34.844305957189796	37.5	35.0	40.0	27.0	41.0
50-51	34.53865099421552	37.0	35.0	40.0	26.5	41.0
52-53	34.02845631585741	37.0	35.0	40.0	23.0	41.0
54-55	33.144844264851514	36.0	34.0	40.0	8.0	41.0
56-57	32.4766774177439	36.0	33.0	40.0	2.0	41.0
58-59	32.14462366047326	35.5	33.0	40.0	2.0	41.0
60-61	31.896131850042728	35.0	33.0	40.0	2.0	41.0
62-63	31.655774723001933	35.0	33.0	39.0	2.0	41.0
64-65	31.389281571749073	35.0	33.0	39.0	2.0	41.0
66-67	31.07544834630341	35.0	33.0	38.5	2.0	40.5
68-69	30.80886344341645	35.0	33.0	37.5	2.0	40.0
70-71	30.54436409404036	35.0	33.0	37.0	2.0	40.0
72-73	30.301279034268706	35.0	32.0	36.5	2.0	39.5
74-75	30.047688353502203	35.0	32.0	36.0	2.0	39.0
76-77	29.788207194270363	35.0	32.0	35.5	2.0	39.0
78-79	29.51836693398311	35.0	31.5	35.0	2.0	37.5
80-81	29.26236621221929	35.0	31.0	35.0	2.0	37.0
82-83	28.999414744711384	35.0	31.0	35.0	2.0	37.0
84-85	28.754511259421328	35.0	31.0	35.0	2.0	36.0
86-87	28.610692398583232	35.0	31.0	35.0	2.0	36.0
88-89	28.41878001656476	35.0	30.5	35.0	2.0	36.0
90-91	28.244597253905393	35.0	30.0	35.0	2.0	35.0
92-93	28.114198276812445	35.0	30.0	35.0	2.0	35.0
94-95	27.929916827555925	34.5	30.0	35.0	2.0	35.0
96-97	27.71572670120957	34.0	29.5	35.0	2.0	35.0
98-99	27.563100154623292	34.0	29.0	35.0	2.0	35.0
100	27.479181919345248	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2076271559220224
1101	2	2.1936109438155817
1101	3	1.0248590703327594
1101	4	0.5574162013411552
1101	5	2.133986206818115
1101	6	0.9311700583582692
1101	7	0.5219791586232105
1101	8	0.367675744149615
1101	9	0.3138219562600284
1101	10-11	0.4330805630480654
1101	12-13	0.39871222176331855
1101	14-15	0.5034935053269933
1101	16-17	0.3983652272655931
1101	18-19	0.34366743980846337
1101	20-21	0.31796326940320796
1101	22-23	0.24670072334772897
1101	24-25	0.14215739273002015
1101	26-27	0.09841782780292618
1101	28-29	0.15747451017664815
1101	30-31	0.19941800666964582
1101	32-33	0.2354575176809348
1101	34-35	0.23335407018241483
1101	36-37	0.25371611618680845
1101	38-39	0.30022344266929935
1101	40-41	0.30496285477893537
1101	42-43	0.37360257830667365
1101	44-45	0.4485645941224874
1101	46-47	0.4169033614781412
1101	48-49	0.40848445439304726
1101	50-51	0.39891159655305586
1101	52-53	0.4802904791637488
1101	54-55	0.5163257127442549
1101	56-57	0.5564344158547314
1101	58-59	0.6109983972787205
1101	60-61	0.6527713925567191
1101	62-63	0.6765914830071083
1101	64-65	0.6861467215005561
1101	66-67	0.6655916596206524
1101	68-69	0.7012880236777832
1101	70-71	0.6193520716409928
1101	72-73	0.6038496748963276
1101	74-75	0.6140253970501242
1101	76-77	0.5670821276496163
1101	78-79	0.6513412234594576
1101	80-81	0.6160988763778228
1101	82-83	0.648615454275653
1101	84-85	0.6302989658696312
1101	86-87	0.6322157344725774
1101	88-89	0.5947489485983475
1101	90-91	0.5797941201989651
1101	92-93	0.6465976146189583
1101	94-95	0.7167854067499846
1101	96-97	0.687882466240513
1101	98-99	0.7311703441051378
1101	100	0.7353907024108679
1102	1	1.487291293025116
1102	2	2.9581734149454526
1102	3	3.1176647431781
1102	4	1.3783487076584677
1102	5	2.536715473309606
1102	6	1.1859407251604779
1102	7	0.7072042875489828
1102	8	0.5704509695027511
1102	9	0.4552219524656351
1102	10-11	0.5251126356374982
1102	12-13	0.4694228725823919
1102	14-15	0.54616943316082
1102	16-17	0.4246019847979028
1102	18-19	0.15203279469965025
1102	20-21	0.014400960498981874
1102	22-23	-0.1715230508568908
1102	24-25	-0.14496730827105608
1102	26-27	-0.10018395753144915
1102	28-29	-0.07144951170869973
1102	30-31	-0.07883349909180026
1102	32-33	-0.10429751651377472
1102	34-35	-0.07497546114575471
1102	36-37	-0.06025261819237926
1102	38-39	-0.0020667188573284534
1102	40-41	0.050153330925930106
1102	42-43	0.08408921366246602
1102	44-45	0.14098582565563333
1102	46-47	0.22456772705663752
1102	48-49	0.1625113181085922
1102	50-51	0.2704142108818175
1102	52-53	0.26537394725394137
1102	54-55	0.28555313007638716
1102	56-57	0.22556119038166855
1102	58-59	0.22952319695438206
1102	60-61	0.1847417260608033
1102	62-63	0.16156639484573532
1102	64-65	0.22258210552235624
1102	66-67	0.22987328456772715
1102	68-69	0.1957755676595525
1102	70-71	0.19995516959292203
1102	72-73	0.18015777011862255
1102	74-75	0.1886500080103879
1102	76-77	0.21545559483788068
1102	78-79	0.2214414533450757
1102	80-81	0.21184731643970878
1102	82-83	0.2672227161207381
1102	84-85	0.2809045829353245
1102	86-87	0.328727020619656
1102	88-89	0.3518292561798049
1102	90-91	0.3969445267116889
1102	92-93	0.42411633524599424
1102	94-95	0.441178737094468
1102	96-97	0.47004617698132023
1102	98-99	0.4680404908417408
1102	100	0.45363033394457375
1103	1	1.2717281930203441
1103	2	2.9504208355912844
1103	3	3.11246320840959
1103	4	1.3154159988705487
1103	5	2.503728243625723
1103	6	1.1586387807616205
1103	7	0.6773106494130872
1103	8	0.5149606021201336
1103	9	0.4191257621896156
1103	10-11	0.44881862172452713
1103	12-13	0.396128639936407
1103	14-15	0.48294186532013583
1103	16-17	0.25900649453258495
1103	18-19	0.18452897357104803
1103	20-21	0.09756171555805793
1103	22-23	-0.029079557659947852
1103	24-25	0.05663399046834172
1103	26-27	0.17770060664197018
1103	28-29	0.23785293525790507
1103	30-31	0.29059247124708065
1103	32-33	0.33687861968763855
1103	34-35	0.34112148851868085
1103	36-37	0.2901708984786069
1103	38-39	0.28290095501832724
1103	40-41	0.3479425215106886
1103	42-43	0.3689819634479079
1103	44-45	0.40011603785266914
1103	46-47	0.3176547467619386
1103	48-49	0.3632373300798477
1103	50-51	0.40090743483772684
1103	52-53	0.3881279905095667
1103	54-55	0.18933476614542855
1103	56-57	0.1931126062873716
1103	58-59	0.2768390062014561
1103	60-61	0.24452010602049157
1103	62-63	0.22680950445498027
1103	64-65	0.21357823967574774
1103	66-67	0.24539073851138582
1103	68-69	0.23603763587143334
1103	70-71	0.22034250221393137
1103	72-73	0.2999542378669737
1103	74-75	0.3040937161572046
1103	76-77	0.3225557641791319
1103	78-79	0.3343545892611992
1103	80-81	0.329174619170292
1103	82-83	0.32314751515127327
1103	84-85	0.2817275361377476
1103	86-87	0.31001326853490596
1103	88-89	0.3065703077853712
1103	90-91	0.31001351852820846
1103	92-93	0.32389969477599934
1103	94-95	0.3705855942536047
1103	96-97	0.4065302326718836
1103	98-99	0.4072730143716008
1103	100	0.442278206227499
1104	1	5.9139675940354905
1104	2	4.9501511764261465
1104	3	3.9730708729914177
1104	4	1.7591337021681426
1104	5	2.73969081541118
1104	6	1.2677457709586406
1104	7	0.6912336397283596
1104	8	0.5359609657970879
1104	9	0.43438512791350803
1104	10-11	0.4990325069826511
1104	12-13	0.4030078766355203
1104	14-15	0.4480230880858258
1104	16-17	0.2666769418097559
1104	18-19	0.13758841779154807
1104	20-21	0.04593201071250519
1104	22-23	-0.010462604628479255
1104	24-25	0.050306959036909404
1104	26-27	0.1534952426816929
1104	28-29	0.15260376916594254
1104	30-31	0.24617548103300635
1104	32-33	0.2868828647964321
1104	34-35	0.32062811212023234
1104	36-37	0.3403762840434581
1104	38-39	0.3589744416417844
1104	40-41	0.3951357465444758
1104	42-43	0.466306493107858
1104	44-45	0.516517849528654
1104	46-47	0.44740927334608216
1104	48-49	0.5065916027913246
1104	50-51	0.6079073254882772
1104	52-53	0.6201034480953425
1104	54-55	0.6338186357743396
1104	56-57	0.5435724804388471
1104	58-59	0.5576191624404032
1104	60-61	0.5486314749335257
1104	62-63	0.6606505167921384
1104	64-65	0.5886972116923346
1104	66-67	0.5917842058953973
1104	68-69	0.5757834144992415
1104	70-71	0.5921182520678627
1104	72-73	0.5100162259310146
1104	74-75	0.5641982595417439
1104	76-77	0.6093182452253849
1104	78-79	0.5716797177847077
1104	80-81	0.6327194366220859
1104	82-83	0.6153995093574594
1104	84-85	0.5956942662346769
1104	86-87	0.616159721755988
1104	88-89	0.5362934698380393
1104	90-91	0.5485101075643435
1104	92-93	0.6101798348115217
1104	94-95	0.6096411068211189
1104	96-97	0.65868905053037
1104	98-99	0.7236574855362257
1104	100	0.7673346955490636
1105	1	1.5252515753433045
1105	2	2.9469253880031694
1105	3	3.0805005406916557
1105	4	1.2859004022935565
1105	5	2.4685335600419407
1105	6	1.1332949974207125
1105	7	0.6632390259160843
1105	8	0.42928099014969234
1105	9	0.3571820482183483
1105	10-11	0.4143173050117923
1105	12-13	0.3196231069978879
1105	14-15	0.3834828589716466
1105	16-17	0.18802785179081383
1105	18-19	0.047442354336176606
1105	20-21	-0.06099396870104101
1105	22-23	-0.1666406990596485
1105	24-25	-0.09292500323294206
1105	26-27	-0.01979489607896312
1105	28-29	0.10433967457530002
1105	30-31	0.09064392660218346
1105	32-33	0.10925884946352937
1105	34-35	0.13264265288960075
1105	36-37	0.11542596916608971
1105	38-39	0.10568194064882874
1105	40-41	0.11301803136709765
1105	42-43	0.10730638803696024
1105	44-45	0.1817862535165773
1105	46-47	0.289688238822464
1105	48-49	0.20814029942778944
1105	50-51	0.18193079951974056
1105	52-53	0.33290361740107244
1105	54-55	0.40228199621000726
1105	56-57	0.434326259824239
1105	58-59	0.433657706321668
1105	60-61	0.40657414571659345
1105	62-63	0.39381024095494155
1105	64-65	0.36925829402693466
1105	66-67	0.3745798146769417
1105	68-69	0.41371169951113984
1105	70-71	0.413376647493628
1105	72-73	0.4200628483486746
1105	74-75	0.4678527148832572
1105	76-77	0.4787250641032692
1105	78-79	0.4910353798021738
1105	80-81	0.4263682173897898
1105	82-83	0.4152501000273112
1105	84-85	0.4184977814151871
1105	86-87	0.48504014287419395
1105	88-89	0.4818520259999275
1105	90-91	0.4166775928152937
1105	92-93	0.483307747082538
1105	94-95	0.49250100632564653
1105	96-97	0.5177811671575796
1105	98-99	0.459466691684586
1105	100	0.48266826585080835
1106	1	-0.5933703849490222
1106	2	1.9326058177080903
1106	3	2.672217098390689
1106	4	1.1700870332272828
1106	5	2.334173839552406
1106	6	1.074890699114846
1106	7	0.5658584977281436
1106	8	0.4199150050748912
1106	9	0.3140327443390589
1106	10-11	0.4011707642821136
1106	12-13	0.3346626183916541
1106	14-15	0.35490679433997485
1106	16-17	0.27599498975875036
1106	18-19	0.18461545331692264
1106	20-21	0.12270781325368318
1106	22-23	0.002758324128812717
1106	24-25	-0.05418852931403961
1106	26-27	-0.06834017709964968
1106	28-29	-0.01808535647834475
1106	30-31	0.013350295504949372
1106	32-33	-0.03001531661357859
1106	34-35	0.0828244455438778
1106	36-37	0.13518233925819345
1106	38-39	0.12427719323765274
1106	40-41	0.10468934941039976
1106	42-43	0.0326933823241049
1106	44-45	0.08326904905801769
1106	46-47	0.08519650500817733
1106	48-49	0.006730760263401692
1106	50-51	0.043640229429769306
1106	52-53	0.025352200303267125
1106	54-55	0.05601048661625185
1106	56-57	0.0658918069839558
1106	58-59	0.06049382821201732
1106	60-61	0.06692428404611306
1106	62-63	0.08193515671029417
1106	64-65	0.13110316614038098
1106	66-67	0.15066154585973734
1106	68-69	0.10121948816714621
1106	70-71	0.09834675551078575
1106	72-73	0.18540668768446622
1106	74-75	0.16532672938358317
1106	76-77	0.15733272842502188
1106	78-79	0.19315457492526988
1106	80-81	0.21039402239460614
1106	82-83	0.23122691679818175
1106	84-85	0.23016486272883085
1106	86-87	0.21640335702008784
1106	88-89	0.2981217635575639
1106	90-91	0.23462640752584107
1106	92-93	0.26495413170249194
1106	94-95	0.31529621639757366
1106	96-97	0.3294506982045675
1106	98-99	0.307998631943704
1106	100	0.2864176968020651
1107	1	-0.8798110659540122
1107	2	1.7055892137690947
1107	3	-0.7008520822004201
1107	4	-0.04632189592539504
1107	5	1.7861097327952749
1107	6	0.7192112927692094
1107	7	0.34458075373694896
1107	8	0.2819587746347807
1107	9	0.27286903230614
1107	10-11	0.2896553282701291
1107	12-13	0.2864427594502743
1107	14-15	0.3592692781601272
1107	16-17	0.32286205367145016
1107	18-19	0.33340552271980073
1107	20-21	0.28608948667059053
1107	22-23	0.26704358822991736
1107	24-25	0.09217126423401822
1107	26-27	0.07862994790862388
1107	28-29	0.05835242502589466
1107	30-31	0.10301301991857059
1107	32-33	0.07095992358329539
1107	34-35	0.10599712377323556
1107	36-37	0.148551672549587
1107	38-39	0.15817031652536429
1107	40-41	0.1321079993936607
1107	42-43	0.14913845681913074
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2216	56-57	0.45498828425674986
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2216	64-65	0.4225892377695395
2216	66-67	0.35069650416150466
2216	68-69	0.3477088479727968
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2216	72-73	0.2854838036020375
2216	74-75	0.25133559227788993
2216	76-77	0.2212397559937962
2216	78-79	0.21099415187594417
2216	80-81	0.1748841258355256
2216	82-83	0.14261400814405434
2216	84-85	0.0940733693403466
2216	86-87	0.061138366432086144
2216	88-89	0.021574689740791797
2216	90-91	0.014790504869971244
2216	92-93	-0.0641760657859578
2216	94-95	-0.15602731920807855
2216	96-97	-0.12079432334734008
2216	98-99	-0.20107839052971244
2216	100	-0.23178211031509122
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2047.0
3	677.0
4	1120.0
5	4638.0
6	15697.0
7	28227.0
8	33885.0
9	45925.0
10	28435.0
11	11744.0
12	10961.0
13	13935.0
14	15060.0
15	16497.0
16	18543.0
17	20579.0
18	28378.0
19	46988.0
20	90437.0
21	102060.0
22	43959.0
23	27075.0
24	27152.0
25	29190.0
26	32043.0
27	36193.0
28	44693.0
29	52629.0
30	60657.0
31	75616.0
32	100911.0
33	153997.0
34	229472.0
35	639487.0
36	558943.0
37	795342.0
38	642480.0
39	158462.0
40	1022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.397951095787263	45.830502573161	24.05134149724567	17.72020483380607
2	13.265679197083388	45.66656552626148	23.588785099568334	17.478970177086797
3	16.552932862190815	29.514487632508835	18.817196702002356	35.115382803298
4	31.109716580497864	31.527934426909166	20.515029365234163	16.847319627358807
5	15.23646622439427	44.32705794000819	21.291783410592018	19.144692425005523
6	34.95271524804558	28.043117342187244	22.61594311647049	14.388224293296686
7	17.23858172227515	27.902684453088266	38.71679769029049	16.141936134346093
8	34.293510061821046	28.263696316460457	21.849043945617076	15.593749676101421
9	33.352743691869044	30.10760970857137	22.178384021694374	14.361262577865219
10-11	14.623337752487778	47.89577815279345	22.137973257048742	15.34291083767004
12-13	14.481140386831484	29.688826511911458	30.970063290960333	24.859969810296725
14-15	14.726419947818172	38.382229062960235	22.024867872935648	24.866483116285952
16-17	24.30571456031298	29.008769053481192	31.051650398713264	15.633865987492568
18-19	15.185496127821924	29.328239056468124	31.324467228059465	24.161797587650486
20-21	24.381977953224805	38.66179476089925	22.145475925973038	14.81075135990291
22-23	23.704299677090784	29.6169916959471	31.417243559482856	15.261465067479262
24-25	15.024053297452438	38.728906546661655	21.511647628497045	24.735392527388864
26-27	15.98900770148739	33.72043746822848	34.494920561185765	15.795634269098366
28-29	24.606268886231742	29.600855657601276	30.144793265547836	15.648082190619142
30-31	21.62010065118926	31.427655426561472	30.343596324846484	16.608647597402783
32-33	17.702117425131135	32.8385694188859	31.124392130701438	18.33492102528152
34-35	17.901544725329295	32.8493346298699	31.205625894548987	18.043494750251813
36-37	18.540567178214417	32.26601095460332	31.848146452097403	17.345275415084863
38-39	19.622694666579978	31.50618022046775	31.209913133934304	17.661211979017967
40-41	17.105437350241075	33.93614981404688	31.479314305528465	17.479098530183578
42-43	16.880280925384085	32.25328748610461	32.60409086872363	18.26234071978767
44-45	17.378795973575528	33.220322174261675	30.856439669119347	18.54444218304345
46-47	18.615381861114173	31.6368939091991	31.936635544135484	17.811088685551248
48-49	17.426085166245954	32.13845380476006	31.85652070265498	18.57894032633901
50-51	18.23379399956091	33.361989524059894	30.5674985795575	17.836717896821693
52-53	18.597419386601686	32.27745474928056	31.080890243427007	18.04423562069075
54-55	18.48340791245363	33.59235797223942	28.50508909448793	19.419145020819023
56-57	18.78671832083438	34.19736518516634	27.871861481651088	19.14405501234819
58-59	20.407012133358585	33.95093136742207	26.498778843462993	19.14327765575635
60-61	19.710583073978906	34.62615979247877	26.117803444679065	19.545453688863258
62-63	18.2965521871404	34.64912353023624	25.672231049278533	21.382093233344836
64-65	19.34712175477179	34.691363521152105	26.826387534403917	19.135127189672183
66-67	19.845030429977133	33.5794255853024	27.33089196250974	19.244652022210726
68-69	20.371194839482932	33.953027874594014	26.76969939385031	18.906077892072755
70-71	17.59930612679487	35.786070052549306	27.258892252722866	19.355731567932956
72-73	17.7502322658214	34.45570412820697	27.70556703012577	20.088496575845863
74-75	18.2852401303372	35.168642748741654	26.27526637798378	20.27085074293737
76-77	19.808804441795658	33.74350137687185	26.79380843460624	19.65388574672625
78-79	19.202459472170343	34.29118979274763	26.16454092133861	20.341809813743414
80-81	19.900177097550646	35.3402190469146	25.677083652327216	19.082520203207544
82-83	19.387913146788787	35.466741607680255	25.7162318160173	19.429113429513652
84-85	19.336455122407354	35.79347854124341	24.70748391943339	20.162582416915846
86-87	19.080622497882878	35.91880626266886	25.491731161759702	19.508840077688568
88-89	21.209762373868006	34.50704756197416	25.12149141280085	19.161698651356982
90-91	19.58075019186583	35.70229725470501	25.30440591790074	19.412546635528415
92-93	18.567138639899216	35.882379823026525	25.465624820383514	20.08485671669074
94-95	19.096247581949875	36.20197938544544	25.101786601010655	19.599986431594033
96-97	19.193683077332597	34.730707485445	25.91378776261129	20.16182167461111
98-99	20.532060629852538	34.863873046249516	24.546796080644285	20.05727024325366
100	18.813230891868287	36.58744225182773	24.311002940763544	20.288323915540442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	468712.0
1	250421.0
2	22195.5
3	9846.5
4	6820.5
5	5911.0
6	5838.0
7	6475.5
8	6299.0
9	5434.5
10	5056.5
11	7605.0
12	48444.5
13	51654.5
14	13688.0
15	10708.5
16	11017.0
17	11786.0
18	12772.5
19	13745.0
20	14900.5
21	16247.0
22	18171.5
23	20423.5
24	22874.0
25	25962.0
26	29764.5
27	33870.5
28	37127.5
29	40385.5
30	44213.5
31	48681.0
32	53725.5
33	58768.5
34	64689.0
35	71105.5
36	76198.5
37	80288.5
38	83934.0
39	85550.5
40	85673.5
41	86119.5
42	86081.5
43	87229.0
44	91975.0
45	97134.5
46	102218.0
47	112811.0
48	123367.5
49	142877.5
50	210186.0
51	221093.5
52	155027.0
53	127585.0
54	127926.5
55	133434.5
56	119991.0
57	95056.0
58	78023.5
59	60854.5
60	48914.5
61	41903.0
62	34543.0
63	26127.5
64	19291.0
65	14366.0
66	10931.5
67	8733.0
68	7070.0
69	5697.0
70	4349.0
71	3156.5
72	2547.5
73	1953.5
74	1292.5
75	882.5
76	669.0
77	456.0
78	295.5
79	172.0
80	83.5
81	45.0
82	23.0
83	11.0
84	7.0
85	7.0
86	3.5
87	3.0
88	2.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14162023727749934
2	0.005465052403256794
3	0.003674776615983017
4	0.0
5	0.002591188639475204
6	0.013026611978452618
7	0.002826751243063859
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.7112520717730984E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0010129191954312163
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	5.889065089716373E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.3556260358865492E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.7112520717730984E-4
74-75	3.1800951484468415E-4
76-77	6.124627693305028E-4
78-79	4.122345562801461E-4
80-81	8.951378936368887E-4
82-83	9.304722841751869E-4
84-85	0.0011189223670461108
86-87	2.3556260358865492E-5
88-89	0.0
90-91	4.7112520717730984E-5
92-93	0.0
94-95	0.0
96-97	0.00120136927830214
98-99	4.5934707699787713E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4245156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.317300774852484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2521397120747	34.76306073733423
2	11.943663434145648	10.34734522660989
3	3.915893900750014	5.0887786170359615
4	1.5254979795349342	2.6432181924377804
5	0.6971862647060487	1.5100113562183917
6	0.3654115850818858	0.9497186122564588
7	0.23596034610289593	0.7154815698154204
8	0.15949313540177093	0.5527049694178227
9	0.11891910543347017	0.463612919214422
>10	0.5922557266474835	4.847163432379745
>50	0.10644141067069993	3.3654669293388366
>100	0.0764736048803585	6.686836334201842
>500	0.0051951819710258935	1.4917629797276106
>1k	0.004538948457360809	4.331309908736599
>5k	4.921751339306901E-4	1.716709485059074
>10k+	4.374890079383912E-4	20.52681873021601
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	424362	9.996381758408878	No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	293009	6.90219629149082	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	52112	1.2275638398211985	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32932	0.7757547661381584	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23632	0.5566815448007093	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	17949	0.42281131718127674	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	11727	0.2762442652284156	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10806	0.2545489494379005	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9961	0.23464390943465918	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9810	0.23108691412047047	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9359	0.22046304069862216	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8758	0.206305728222944	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8536	0.20107623842327585	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8274	0.19490449820925307	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6583	0.15507086194241154	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5958	0.1403481992181206	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	5231	0.12322279793722539	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4843	0.11408296891798558	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	4770	0.1123633619117884	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	4396	0.1035533205375727	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	4386	0.10331775793398405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0020258383908624326	0.0	0.0	0.0	0.0
2	0.0020258383908624326	0.0	0.0	0.0	0.0
3	0.0020258383908624326	0.0	0.0	0.0	0.0
4	0.0020258383908624326	0.0	0.0	0.0	0.0
5	0.0020258383908624326	0.0	0.0	0.0	0.0
6	0.0020258383908624326	0.0	0.0	0.0	0.0
7	0.0020493946512212978	0.0	0.0	0.0	0.0
8	0.0020493946512212978	0.0	0.0	0.0	0.0
9	0.0020493946512212978	0.0	0.0	0.0	0.0
10-11	0.0020729509115801634	0.0	0.0	0.0	0.0
12-13	0.002131841562477327	0.0	0.0	0.0	0.0
14-15	0.0021907322133744907	0.0	0.0	0.0	0.0
16-17	0.00227317912463052	0.0	0.0	0.0	0.0
18-19	0.002508741728219175	0.0	0.0	0.0	0.0
20-21	0.0026736355507312333	0.0	0.0	0.0	0.0
22-23	0.0027678605921666955	0.0	0.0	0.0	0.0
24-25	0.002862085633602157	0.0	0.0	0.0	0.0
26-27	0.002932754414678754	0.0	0.0	0.0	0.0
28-29	0.003074091976831947	0.0	0.0	0.0	0.0
30-31	0.003250763929523438	0.0	0.0	0.0	0.0
32-33	0.0034038796218560635	0.0	0.0	0.0	0.0
34-35	0.003721889136700748	0.0	0.0	0.0	0.0
36-37	0.004569914509619906	0.0	0.0	0.0	0.0
38-39	0.006265965255458221	0.0	0.0	0.0	0.0
40-41	0.009552063575519958	0.0	0.0	0.0	0.0
42-43	0.013756856049577448	0.0	0.0	0.0	0.0
44-45	0.01818543299704416	0.0	0.0	0.0	0.0
46-47	0.022036881565718668	0.0	0.0	0.0	0.0
48-49	0.026500792903723678	0.0	0.0	0.0	0.0
50-51	0.032413414253798914	0.0	0.0	0.0	0.0
52-53	0.03970407683486779	0.0	0.0	0.0	0.0
54-55	0.04993927196079485	0.0	0.0	0.0	0.0
56-57	0.06077515172587297	0.0	0.0	0.0	0.0
58-59	0.0721881598697433	0.0	0.0	0.0	0.0
60-61	0.09009091774248107	0.0	0.0	0.0	0.0
62-63	0.11107954572223022	0.0	0.0	0.0	0.0
64-65	0.1349891499864787	0.0	0.0	0.0	0.0
66-67	0.16198462435773858	0.0	0.0	1.1778130179432746E-5	0.0
68-69	0.1960587549668375	0.0	0.0	2.3556260358865492E-5	0.0
70-71	0.24556223611099331	0.0	0.0	2.3556260358865492E-5	0.0
72-73	0.29314588203590164	0.0	0.0	4.7112520717730984E-5	0.0
74-75	0.34744306216308657	0.0	0.0	5.889065089716373E-5	0.0
76-77	0.4029298334383943	0.0	0.0	7.066878107659648E-5	0.0
78-79	0.46611950185105094	0.0	0.0	8.244691125602923E-5	0.0
80-81	0.543961164206922	0.0	0.0	9.422504143546197E-5	0.0
82-83	0.6415311946133428	0.0	0.0	9.422504143546197E-5	0.0
84-85	0.7615267848814037	0.0	0.0	9.422504143546197E-5	0.0
86-87	0.8944783183468406	0.0	0.0	9.422504143546197E-5	0.0
88	1.0015415216778842	0.0	2.3556260358865492E-5	9.422504143546197E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	6160	0.0	83.37578	1
AACGTGT	2580	0.0	76.26977	1
ACGTGTG	2980	0.0	70.36743	2
ATCGAGT	2220	0.0	67.64467	1
CGTGTGG	3255	0.0	67.01926	3
TCGAGTG	2350	0.0	66.22366	2
GTGGAAT	126745	0.0	57.461716	6
TGGAATC	127870	0.0	56.98385	7
GGAATCA	128820	0.0	56.651493	8
GAATCAC	129220	0.0	56.49795	9
AGTGGAA	129365	0.0	54.623962	5
ATGAGTG	3085	0.0	54.56083	2
ACGAGTG	123390	0.0	54.37854	2
CGAGTGG	127280	0.0	54.34307	3
GAGTGGA	130775	0.0	54.027832	4
AACGAGT	114860	0.0	53.85865	1
AATGAGT	2925	0.0	52.306217	1
TGAGTGG	3540	0.0	51.92854	3
AGCGAGT	1265	0.0	50.238785	1
GTGTGGA	5010	0.0	45.792492	4
>>END_MODULE