FastQCFastQC Report
Thu 26 May 2016
SRR2132775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739637
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2423248.845113421960647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA654072.387433079637923No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG213010.7775117652448116No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT190640.6958586119255945No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79880.2915714746150676No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC75690.2762774776366358No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67270.245543478935348No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA55320.2019245615386272No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55310.20188806035252113No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53650.1958288634589181No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33580.12257098294409076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT47300.087.1922761
AACGTGT21650.078.067741
CGTGTGG24350.074.714423
GTGGAAT778200.074.327776
TCGAGTG16400.074.243122
TGGAATC782600.073.783637
GGAATCA786700.073.45758
GAATCAC790450.073.126849
ACGAGTG729750.072.666682
CGAGTGG752500.072.505083
AACGAGT674600.072.36451
AGTGGAA772600.072.347635
ATCGAGT16150.072.2961
GAGTGGA776600.072.023434
ACGTGTG24750.070.2792052
GCGAGTG8350.057.426722
AGCGAGT8050.057.31461
GTGTGGA36750.054.7453354
TGAGTGG29650.054.224283
ATGAGTG30750.048.0048182