##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132775_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2739637
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.05431303490207	31.0	25.0	33.0	16.0	34.0
2	28.320189134545927	31.0	26.0	34.0	16.0	34.0
3	29.357955816774265	31.0	28.0	34.0	16.0	34.0
4	34.83038190826011	35.0	35.0	37.0	32.0	37.0
5	33.91235006681542	35.0	35.0	37.0	28.0	37.0
6	35.33576163557435	37.0	35.0	37.0	32.0	37.0
7	35.69993944453225	37.0	35.0	37.0	33.0	37.0
8	35.97932390313023	37.0	35.0	37.0	35.0	37.0
9	37.95417166580828	39.0	38.0	39.0	35.0	39.0
10-11	37.94799329254204	39.0	38.5	39.0	35.0	39.0
12-13	37.93742820672958	39.0	38.5	39.0	35.0	39.0
14-15	39.38244172494385	41.0	39.0	41.0	36.0	41.0
16-17	39.36293603860658	41.0	39.0	41.0	36.0	41.0
18-19	39.238967425246486	41.0	39.0	41.0	35.5	41.0
20-21	39.157531453984596	40.5	39.0	41.0	35.0	41.0
22-23	39.018668714139864	40.0	39.0	41.0	35.0	41.0
24-25	38.88479440889432	40.0	39.0	41.0	35.0	41.0
26-27	38.72236139313347	40.0	39.0	41.0	35.0	41.0
28-29	38.57279942561733	40.0	39.0	41.0	35.0	41.0
30-31	38.437650316447034	40.0	38.5	41.0	35.0	41.0
32-33	38.27626251215033	40.0	38.0	41.0	34.0	41.0
34-35	38.18335312305973	40.0	38.0	41.0	34.0	41.0
36-37	37.99007149487323	40.0	38.0	41.0	34.0	41.0
38-39	37.42452357739364	40.0	36.0	41.0	33.5	41.0
40-41	37.14636190853022	40.0	35.0	41.0	33.0	41.0
42-43	36.97052364966599	40.0	35.0	41.0	33.0	41.0
44-45	36.8219118080242	40.0	35.0	41.0	33.0	41.0
46-47	36.68291383128495	39.5	35.0	41.0	32.5	41.0
48-49	36.50662514778418	39.0	35.0	41.0	32.0	41.0
50-51	36.18629858627256	39.0	35.0	41.0	31.0	41.0
52-53	35.6210443208352	39.0	35.0	41.0	29.0	41.0
54-55	34.54407061957478	39.0	35.0	41.0	21.0	41.0
56-57	33.670858767055634	39.0	34.5	41.0	4.5	41.0
58-59	33.28830279339927	38.5	34.0	41.0	2.0	41.0
60-61	33.04314038684687	38.0	34.0	40.0	2.0	41.0
62-63	32.786896037686745	37.5	34.0	40.0	2.0	41.0
64-65	32.522195458741436	37.0	34.0	40.0	2.0	41.0
66-67	32.21895053979779	36.5	33.0	40.0	2.0	41.0
68-69	31.922659826831072	36.0	33.0	39.5	2.0	41.0
70-71	31.605305739410003	35.5	33.0	39.0	2.0	41.0
72-73	31.29889069245305	35.0	33.0	39.0	2.0	40.5
74-75	30.97239086784125	35.0	33.0	38.0	2.0	40.0
76-77	30.645098784985016	35.0	33.0	37.0	2.0	39.5
78-79	30.302626223839145	35.0	32.0	37.0	2.0	39.0
80-81	29.97637551982252	35.0	32.0	36.0	2.0	39.0
82-83	29.66155333717569	35.0	32.0	36.0	2.0	37.5
84-85	29.354065337853154	35.0	31.0	35.5	2.0	37.0
86-87	29.141842696678427	35.0	31.0	35.0	2.0	37.0
88-89	28.911697060595984	35.0	31.0	35.0	2.0	36.0
90-91	28.70210761498695	35.0	31.0	35.0	2.0	36.0
92-93	28.529934987737427	35.0	31.0	35.0	2.0	36.0
94-95	28.33353506322188	35.0	31.0	35.0	2.0	36.0
96-97	28.130886500656835	35.0	30.5	35.0	2.0	35.0
98-99	27.965856425504548	34.5	30.0	35.0	2.0	35.0
100	27.8621901368685	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.25315651712455534
1101	2	2.340573604703227
1101	3	1.1548407078446772
1101	4	0.5799831355954908
1101	5	2.1960235590016666
1101	6	0.9612803251155739
1101	7	0.5353491726952058
1101	8	0.3867903407464439
1101	9	0.3127271511729006
1101	10-11	0.431153223414249
1101	12-13	0.3709188347946224
1101	14-15	0.4735924047452116
1101	16-17	0.40101031780640994
1101	18-19	0.38731205655221146
1101	20-21	0.3298325631487913
1101	22-23	0.3370681553271595
1101	24-25	0.29412775966323323
1101	26-27	0.22802608309685723
1101	28-29	0.3260850307050731
1101	30-31	0.3987267472919527
1101	32-33	0.3671706269605579
1101	34-35	0.3594051026338789
1101	36-37	0.3485478904419068
1101	38-39	0.373267205951322
1101	40-41	0.40938025064314587
1101	42-43	0.3689146110772583
1101	44-45	0.4712262510595764
1101	46-47	0.36532475660424524
1101	48-49	0.29560276530095564
1101	50-51	0.3160052174087653
1101	52-53	0.3422353513636551
1101	54-55	0.36676500219635244
1101	56-57	0.28612979861631516
1101	58-59	0.2482614075572016
1101	60-61	0.32811352289331097
1101	62-63	0.3296433197070314
1101	64-65	0.3364098273665661
1101	66-67	0.28525546439031046
1101	68-69	0.31461094360484765
1101	70-71	0.2194230710178111
1101	72-73	0.32952326075459126
1101	74-75	0.31134290174237833
1101	76-77	0.26539834978814625
1101	78-79	0.3416974875542991
1101	80-81	0.31452894414968213
1101	82-83	0.2594100700446944
1101	84-85	0.2461750513893719
1101	86-87	0.18873139370466774
1101	88-89	0.22564208047798218
1101	90-91	0.2386576388309365
1101	92-93	0.2530084816469049
1101	94-95	0.2770783141395121
1101	96-97	0.2604302069207769
1101	98-99	0.26380716939581816
1101	100	0.29406426297818555
1102	1	1.2551380056143202
1102	2	2.93392499780332
1102	3	3.1383478327766774
1102	4	1.3517292238510095
1102	5	2.5602904007812555
1102	6	1.1465407731792823
1102	7	0.6462321516891336
1102	8	0.49345236033894935
1102	9	0.4187503975132074
1102	10-11	0.49190282340187963
1102	12-13	0.4369228197105812
1102	14-15	0.5280796734074258
1102	16-17	0.4569511683306757
1102	18-19	0.39042873750414486
1102	20-21	0.37583189101832204
1102	22-23	0.3565346592465062
1102	24-25	0.42698269865044125
1102	26-27	0.47413233854732084
1102	28-29	0.48789490653545187
1102	30-31	0.45437967367721654
1102	32-33	0.46965170085351815
1102	34-35	0.46010749891861025
1102	36-37	0.4558011155961381
1102	38-39	0.500802416762447
1102	40-41	0.5522438505400373
1102	42-43	0.6404821709095927
1102	44-45	0.60079203882092
1102	46-47	0.6662416313172344
1102	48-49	0.695512815496329
1102	50-51	0.7879299088794411
1102	52-53	0.8255374465857983
1102	54-55	0.8204273902639443
1102	56-57	0.6807174779861427
1102	58-59	0.7214543962591264
1102	60-61	0.8146910325502006
1102	62-63	0.8188380994272464
1102	64-65	0.8271584101834648
1102	66-67	0.8148068422328478
1102	68-69	0.7484755741350284
1102	70-71	0.8041620196322405
1102	72-73	0.7761660987008483
1102	74-75	0.822913991755506
1102	76-77	0.8633523407622512
1102	78-79	0.8204910731958321
1102	80-81	0.7448206128731307
1102	82-83	0.7473333115484522
1102	84-85	0.677776003341517
1102	86-87	0.6975177295116204
1102	88-89	0.7436458779154123
1102	90-91	0.7660733570108214
1102	92-93	0.7339732032737025
1102	94-95	0.6860891754288367
1102	96-97	0.6800519540311889
1102	98-99	0.7603286449116702
1102	100	0.7368006417406576
1103	1	1.6048124552908085
1103	2	3.0817132898378112
1103	3	3.241221832105719
1103	4	1.4244067860517262
1103	5	2.607252036286269
1103	6	1.1813145289342515
1103	7	0.6932599604982954
1103	8	0.4783852378872666
1103	9	0.389349982024477
1103	10-11	0.4538830801620719
1103	12-13	0.441531817046652
1103	14-15	0.5582110296008906
1103	16-17	0.41474874307665033
1103	18-19	0.38562185140233396
1103	20-21	0.36017384533009533
1103	22-23	0.3344090591109605
1103	24-25	0.421885970563487
1103	26-27	0.4383110734671547
1103	28-29	0.463395903504491
1103	30-31	0.4957154374108441
1103	32-33	0.526679932045468
1103	34-35	0.5061520782666165
1103	36-37	0.5059178777358824
1103	38-39	0.5250588895359911
1103	40-41	0.5312746325356557
1103	42-43	0.4973379291279656
1103	44-45	0.6488272305899869
1103	46-47	0.6016749596499409
1103	48-49	0.5764540057322023
1103	50-51	0.6538986486688216
1103	52-53	0.737458472655554
1103	54-55	0.8198596009814949
1103	56-57	0.911162392715795
1103	58-59	0.7939379051240678
1103	60-61	0.7963267997308634
1103	62-63	0.8429973270590381
1103	64-65	0.8448493551217382
1103	66-67	0.9255971689872027
1103	68-69	0.859164092071488
1103	70-71	0.8353571493158931
1103	72-73	0.905376466207855
1103	74-75	0.9467143639378648
1103	76-77	0.9452100531079175
1103	78-79	0.9473179637350917
1103	80-81	0.9360285451248735
1103	82-83	0.9319230637651117
1103	84-85	0.888908338783363
1103	86-87	0.9046482792938022
1103	88-89	0.8607184932853897
1103	90-91	0.9005484104519539
1103	92-93	0.9323328772718646
1103	94-95	0.9310710493810745
1103	96-97	0.9362665157507806
1103	98-99	0.9612477158831467
1103	100	1.0127636138201837
1104	1	5.9901587526586475
1104	2	5.025992226799911
1104	3	4.071681731360396
1104	4	1.8107497956677179
1104	5	2.8257667914502917
1104	6	1.2226708146827932
1104	7	0.6897181355992856
1104	8	0.5082325273986967
1104	9	0.45721154102415085
1104	10-11	0.47959065728232986
1104	12-13	0.40554146082039466
1104	14-15	0.5421586944691299
1104	16-17	0.4017997353973968
1104	18-19	0.3737100678020866
1104	20-21	0.3850367344604564
1104	22-23	0.34241338571855806
1104	24-25	0.3368694460254389
1104	26-27	0.3905513077849818
1104	28-29	0.3963338121541824
1104	30-31	0.4331451244432003
1104	32-33	0.45877557688845627
1104	34-35	0.4259362289941322
1104	36-37	0.4149552513683403
1104	38-39	0.5072937757327125
1104	40-41	0.48599900197297075
1104	42-43	0.5703988482924558
1104	44-45	0.5902466569872189
1104	46-47	0.47984996367315347
1104	48-49	0.527088078820583
1104	50-51	0.5746401186674106
1104	52-53	0.6077820134697518
1104	54-55	0.5926344292327741
1104	56-57	0.5114127642218733
1104	58-59	0.5517631218880013
1104	60-61	0.5747233554513684
1104	62-63	0.6458021443213084
1104	64-65	0.5673936155789647
1104	66-67	0.5794070712534563
1104	68-69	0.49410084233456075
1104	70-71	0.5712976792535294
1104	72-73	0.5147476405437388
1104	74-75	0.5861670877967313
1104	76-77	0.5174688473452989
1104	78-79	0.5217601545179349
1104	80-81	0.5937773255276255
1104	82-83	0.5751110343713286
1104	84-85	0.6152889157799954
1104	86-87	0.6658215261571421
1104	88-89	0.6064495999007775
1104	90-91	0.6761698358306525
1104	92-93	0.6865124022102798
1104	94-95	0.7407176575478474
1104	96-97	0.698369669967434
1104	98-99	0.7076930707845008
1104	100	0.7321822163680736
1105	1	1.5250095392679945
1105	2	3.0057427697201398
1105	3	3.1384082031868807
1105	4	1.3201879484129009
1105	5	2.4981426951398333
1105	6	1.117672633474605
1105	7	0.6788805638530206
1105	8	0.47516946459676035
1105	9	0.36996478604584837
1105	10-11	0.4189605346841745
1105	12-13	0.3639850116092518
1105	14-15	0.46565952270778865
1105	16-17	0.3921949657426751
1105	18-19	0.3634947619805686
1105	20-21	0.2920023176295956
1105	22-23	0.2717178174113286
1105	24-25	0.301459480567992
1105	26-27	0.28439908143162995
1105	28-29	0.27839852310033564
1105	30-31	0.31592480716185634
1105	32-33	0.35149077220569325
1105	34-35	0.4470022855563798
1105	36-37	0.449394635964552
1105	38-39	0.44157411568012606
1105	40-41	0.4480050931140056
1105	42-43	0.47424585565530464
1105	44-45	0.520113211289754
1105	46-47	0.5873192289899549
1105	48-49	0.5537920277002115
1105	50-51	0.43775837201858536
1105	52-53	0.5513441697742536
1105	54-55	0.5919539221159411
1105	56-57	0.5529602009934962
1105	58-59	0.5460299875589953
1105	60-61	0.5836150101678754
1105	62-63	0.6740444577520392
1105	64-65	0.6415015471054346
1105	66-67	0.5659441169993258
1105	68-69	0.5742731945975805
1105	70-71	0.5886617517385382
1105	72-73	0.5954140013007354
1105	74-75	0.6113631513805302
1105	76-77	0.5630230154581817
1105	78-79	0.5534655091569789
1105	80-81	0.5809763100476069
1105	82-83	0.5986138009638182
1105	84-85	0.5596633238574569
1105	86-87	0.5650521264961199
1105	88-89	0.5546073757430179
1105	90-91	0.5762332489855169
1105	92-93	0.5856280599788768
1105	94-95	0.5769465956125508
1105	96-97	0.5836910870743672
1105	98-99	0.44459727154143636
1105	100	0.5085969066988412
1106	1	-0.45519731121390805
1106	2	1.9700296300034452
1106	3	2.771539941652726
1106	4	1.2428148270155006
1106	5	2.430515553999598
1106	6	1.1106508457683688
1106	7	0.5860224625251647
1106	8	0.47134881280333474
1106	9	0.3577779350972108
1106	10-11	0.43194611990971765
1106	12-13	0.389542867610281
1106	14-15	0.44879385008638195
1106	16-17	0.4090728536566104
1106	18-19	0.4071314332367848
1106	20-21	0.40880640329854145
1106	22-23	0.36468822650329713
1106	24-25	0.40880309565282147
1106	26-27	0.4062931154098308
1106	28-29	0.4850280358304957
1106	30-31	0.4981423312569717
1106	32-33	0.4920488615734939
1106	34-35	0.5930233454499643
1106	36-37	0.5163329804605965
1106	38-39	0.6114038746122361
1106	40-41	0.5680630045396384
1106	42-43	0.5897326952273119
1106	44-45	0.5794946653398299
1106	46-47	0.6076064597430246
1106	48-49	0.6328262465632477
1106	50-51	0.7070280871360879
1106	52-53	0.6965746996918796
1106	54-55	0.4925748600753792
1106	56-57	0.4992196596409002
1106	58-59	0.5428560169982148
1106	60-61	0.4826377503022883
1106	62-63	0.46421599692265403
1106	64-65	0.483715155014238
1106	66-67	0.48638263506192203
1106	68-69	0.4890594962591521
1106	70-71	0.5414835572390011
1106	72-73	0.5419407378413048
1106	74-75	0.5189803525543653
1106	76-77	0.45852446962494753
1106	78-79	0.4823641200412041
1106	80-81	0.49091605766142976
1106	82-83	0.4887122671606434
1106	84-85	0.46586920295012746
1106	86-87	0.472742437107307
1106	88-89	0.4977690726695805
1106	90-91	0.49701507146340873
1106	92-93	0.5318111274685471
1106	94-95	0.5054127486778235
1106	96-97	0.5002418603935439
1106	98-99	0.5209444906845135
1106	100	0.532314264520128
1107	1	-0.9919222118475872
1107	2	1.6257399614998924
1107	3	-0.6898702702828601
1107	4	-0.12077607021876702
1107	5	1.7845330723812225
1107	6	0.7138078013342621
1107	7	0.2811864437258791
1107	8	0.22960725167354212
1107	9	0.20388262619722752
1107	10-11	0.2666211264499907
1107	12-13	0.21108296046691777
1107	14-15	0.2655774688333423
1107	16-17	0.17214819726977737
1107	18-19	0.12372897439846042
1107	20-21	0.11792464960156224
1107	22-23	0.12899154544547287
1107	24-25	0.03400035577380578
1107	26-27	0.02916934199870269
1107	28-29	0.09051420027525836
1107	30-31	0.12574108753334912
1107	32-33	0.08489706345618231
1107	34-35	0.09959773460334986
1107	36-37	0.20566903903700506
1107	38-39	0.2097212639476851
1107	40-41	0.23801433588768361
1107	42-43	0.24612098289089346
1107	44-45	0.3327640151903637
1107	46-47	0.2864922737666049
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2216	90-91	0.4282890831334818
2216	92-93	0.32436715961285856
2216	94-95	0.2771698464021881
2216	96-97	0.3417648839548306
2216	98-99	0.3266527170126565
2216	100	0.2830657579243834
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1195.0
3	318.0
4	349.0
5	829.0
6	2534.0
7	4417.0
8	5291.0
9	7294.0
10	5545.0
11	5675.0
12	5254.0
13	7666.0
14	8223.0
15	9021.0
16	11282.0
17	13360.0
18	19798.0
19	35639.0
20	72831.0
21	87523.0
22	37888.0
23	21022.0
24	18775.0
25	19396.0
26	20289.0
27	22705.0
28	26375.0
29	31062.0
30	35613.0
31	43572.0
32	56066.0
33	78657.0
34	115508.0
35	227015.0
36	321426.0
37	526206.0
38	630198.0
39	202986.0
40	834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.22986084103596	45.14098654026662	23.135287426567988	18.493865192129434
2	14.034091883894712	45.662222634303824	23.702901172084182	16.600784309717287
3	16.431062193083605	24.655956144606282	19.252329489269947	39.66065217304016
4	36.71928069302612	25.704682773666732	20.684273135455538	16.891763397851616
5	16.530009216758074	45.01917268189409	20.720403712254637	17.7304143890932
6	39.40643981804117	23.733207851199996	21.848749005676996	15.011603325081838
7	16.814336634964437	23.193547857224985	43.70735393156917	16.284761576241397
8	38.58292175204233	23.582065799228147	21.71992128884228	16.11509115988724
9	38.098149499367985	24.126225481697027	22.18903453267714	15.586590486257851
10-11	15.429197371768597	46.597049171112815	22.0353645391707	15.938388917947888
12-13	15.274414092085923	24.12870756235224	33.12677920469025	27.470099140871586
14-15	15.53729563442164	35.10510699045166	22.09371168516121	27.263885689965495
16-17	26.96678428565536	23.69401493701538	33.304320973910045	16.034879803419212
18-19	15.69363021451382	23.936054302084546	33.36551521241683	27.00480027098481
20-21	27.007975874176033	35.261368568171626	22.0915398645879	15.639115693064445
22-23	26.71687891498034	24.142888273154437	33.358397481126154	15.781835330739073
24-25	15.67919399540888	35.177616596651305	22.00074316414912	27.14244624379069
26-27	16.012051233804193	28.568899560215378	39.53303659080319	15.886012615177233
28-29	25.51929324943414	23.963849955304298	34.69457815031699	15.822278644944568
30-31	22.839613423238188	25.48425211077234	35.10202264022569	16.57411182576378
32-33	18.287806012256368	27.40593005569716	36.20032507956346	18.10593885248301
34-35	18.289558069189457	27.19940634470917	36.46930231997889	18.041733266122485
36-37	18.49465823391931	27.10840888774681	36.82018822201628	17.576744656317608
38-39	19.399796396383902	26.523294874466945	36.19419288029764	17.882715848851507
40-41	17.79929238800615	28.31035644503268	36.11879968039561	17.771551486565556
42-43	17.778916219188623	27.082985808579537	36.90294943117878	18.23514854105306
44-45	17.98305760945702	27.928663541921793	35.729970795400995	18.358308053220192
46-47	18.79584777107332	26.87224621364071	36.3660404644849	17.965865550801073
48-49	17.986908484591208	27.179859959549386	36.28124820916055	18.55198334669885
50-51	18.551326325348942	28.04008341251049	35.19944430594272	18.209145956197847
52-53	18.96109902211444	27.573923611370926	35.1169708525094	18.348006514005238
54-55	19.194677981060995	28.62558068824446	32.68230426147697	19.497437069217565
56-57	19.692207398279407	29.542380979669936	31.149528203918987	19.61588341813167
58-59	20.80781139983144	30.09588496578196	29.44512356929038	19.651180065096217
60-61	20.430991405065708	30.416748642247132	29.275009791443175	19.87725016124399
62-63	19.42025531119634	30.726534208729113	28.824019386509963	21.029191093564588
64-65	19.98091352978515	30.626393204647183	29.80476975599322	19.58792350957444
66-67	20.1548964333596	29.877042104483188	30.180220956279975	19.787840505877238
68-69	20.569312649814556	30.037866330466407	29.67290922118514	19.719911798533893
70-71	18.83837165288686	31.302796684378258	30.05914652196623	19.79968514076865
72-73	18.85931468722909	30.496473057443996	30.356745905005244	20.28746635032167
74-75	19.124250834464416	30.894738945730477	29.505701770475333	20.475308449329773
76-77	20.192785720073793	30.013775623060408	29.746092709140164	20.047345947725635
78-79	19.773761461627764	30.378622226610485	29.196492008685116	20.65112430307664
80-81	20.082683180915268	31.237320294026823	28.782193403888286	19.897803121169623
82-83	19.980332995816198	31.200052270137324	28.760858281495132	20.058756452551346
84-85	19.874430711311092	31.512120551773375	28.114894749348718	20.49855398756681
86-87	19.6951493382425	31.661189358515262	28.417638617385922	20.226022685856318
88-89	21.109292946474294	30.751939034258918	28.141903471153295	19.996864548113493
90-91	20.158429834917822	31.559453803252296	28.10492490698124	20.177191454848646
92-93	19.35484518569431	31.774556264205806	28.30455275644182	20.566045793658063
94-95	19.795564119546516	32.014265396157484	27.891127162307843	20.299043321988155
96-97	19.599720105942165	31.06829517215602	28.362050337128526	20.969934384773296
98-99	20.572753883882218	31.165906556138097	27.36408853260311	20.897251027376573
100	19.63008237952692	32.1209342697591	27.12545494165833	21.123528409055652
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70980.0
1	38454.0
2	4279.5
3	2197.5
4	1720.5
5	1582.0
6	1530.5
7	1675.0
8	1625.0
9	1476.5
10	1486.0
11	2423.5
12	19624.0
13	22268.5
14	7898.5
15	7515.0
16	8564.5
17	9921.5
18	11422.5
19	13230.5
20	15112.5
21	17285.0
22	19874.0
23	22591.0
24	25717.5
25	29405.5
26	33413.5
27	37744.5
28	42049.0
29	46036.5
30	50357.0
31	54532.0
32	58638.5
33	63173.0
34	67752.0
35	72090.0
36	76470.5
37	80534.5
38	82464.5
39	82879.0
40	82944.5
41	83376.0
42	83165.5
43	82459.0
44	82881.0
45	83296.5
46	84301.5
47	85036.5
48	83641.5
49	85830.0
50	103636.0
51	104448.0
52	79615.5
53	66349.0
54	63056.0
55	62965.5
56	55876.0
57	43796.0
58	35737.0
59	27204.0
60	20625.0
61	16575.0
62	13077.5
63	9785.5
64	6974.5
65	5087.0
66	3907.5
67	3073.5
68	2366.0
69	1725.0
70	1275.5
71	948.5
72	684.5
73	501.0
74	345.5
75	218.0
76	139.5
77	80.5
78	49.5
79	33.0
80	21.5
81	12.5
82	5.5
83	2.5
84	1.0
85	0.0
86	1.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13771897517809842
2	0.005767187404754718
3	0.0032121043773317417
4	0.0
5	0.002263073538574636
6	0.012264398531630286
7	0.0029930972606954866
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.8250593053021256E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.760284665450204E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	5.475177915906378E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.380142332725102E-4
74-75	2.0075652358323386E-4
76-77	5.475177915906377E-4
78-79	2.3725770968927636E-4
80-81	8.395272804389779E-4
82-83	8.395272804389779E-4
84-85	0.0012410403276054455
86-87	1.8250593053021256E-5
88-89	0.0
90-91	5.475177915906378E-5
92-93	0.0
94-95	1.8250593053021256E-5
96-97	0.0010585343970752328
98-99	4.92766012431574E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2739637.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.95193284417307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.38302725102716	59.35504249105954
2	6.2720369244193375	8.147618422221173
3	1.0116268204579928	1.9712135191725568
4	0.373499838595171	0.9703814573497211
5	0.1842917249948311	0.5985051872800541
6	0.1097430512185579	0.4276813975717451
7	0.08000427602660172	0.3637502654608564
8	0.04943320174295313	0.2568625599904601
9	0.043220336047943286	0.25265199280397443
>10	0.42929948995748163	6.676416500481236
>50	0.037880898065267975	1.6985840466104636
>100	0.02148754838659161	2.708495346119618
>500	0.0025903467944871166	1.225247998209103
>1k	0.0012951733972421267	1.1997913395988284
>5k	3.3787132101968523E-4	1.415916908165407
>10k+	2.2524754734645682E-4	12.731840567905184
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	242324	8.845113421960647	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65407	2.387433079637923	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	21301	0.7775117652448116	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19064	0.6958586119255945	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7988	0.2915714746150676	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	7569	0.2762774776366358	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6727	0.245543478935348	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	5532	0.2019245615386272	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5531	0.20188806035252113	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5365	0.1958288634589181	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3358	0.12257098294409076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.205201638027227E-4	0.0	0.0	0.0	0.0
2	6.205201638027227E-4	0.0	0.0	0.0	0.0
3	6.205201638027227E-4	0.0	0.0	0.0	0.0
4	6.205201638027227E-4	0.0	0.0	0.0	0.0
5	6.205201638027227E-4	0.0	0.0	0.0	0.0
6	6.205201638027227E-4	0.0	0.0	0.0	0.0
7	6.205201638027227E-4	0.0	0.0	0.0	0.0
8	6.205201638027227E-4	0.0	0.0	0.0	0.0
9	6.205201638027227E-4	0.0	0.0	0.0	0.0
10-11	6.205201638027227E-4	0.0	0.0	0.0	0.0
12-13	6.205201638027227E-4	0.0	0.0	1.825059305302126E-5	0.0
14-15	6.205201638027227E-4	0.0	0.0	3.650118610604252E-5	0.0
16-17	6.570213499087652E-4	0.0	0.0	3.650118610604252E-5	0.0
18-19	7.482743151738715E-4	0.0	0.0	3.650118610604252E-5	0.0
20-21	8.395272804389779E-4	0.0	0.0	3.650118610604252E-5	0.0
22-23	9.307802457040842E-4	0.0	0.0	9.125296526510629E-5	0.0
24-25	9.490308387571054E-4	0.0	0.0	2.1900711663625508E-4	0.0
26-27	9.490308387571054E-4	0.0	0.0	2.1900711663625508E-4	0.0
28-29	0.0010037826179161691	0.0	0.0	2.1900711663625508E-4	0.0
30-31	0.001058534397075233	0.0	0.0	2.3725770968927634E-4	0.0
32-33	0.0011315367692873181	0.0	0.0	2.555083027422976E-4	0.0
34-35	0.0012957921067645092	0.0	0.0	2.7375889579531887E-4	0.0
36-37	0.0018068087122491046	0.0	0.0	2.9200948884834015E-4	0.0
38-39	0.0029565960745894437	0.0	0.0	2.9200948884834015E-4	0.0
40-41	0.004635650635467399	0.0	0.0	3.4676126800740393E-4	0.0
42-43	0.007117731290678291	0.0	0.0	4.0151304716646766E-4	0.0
44-45	0.009928322620843564	0.0	0.0	4.745154193785527E-4	0.0
46-47	0.013341183521758539	0.0	0.0	4.745154193785527E-4	0.0
48-49	0.01715555746983998	0.0	0.0	4.745154193785527E-4	0.0
50-51	0.021407945651193937	0.0	0.0	5.292671985376164E-4	0.0
52-53	0.026974376532365417	0.0	0.0	5.475177915906377E-4	0.0
54-55	0.0348586327312706	0.0	0.0	5.475177915906377E-4	0.0
56-57	0.04272463833712277	0.0	0.0	5.475177915906377E-4	0.0
58-59	0.052141944352481734	0.0	0.0	5.475177915906377E-4	0.0
60-61	0.06449759584937713	0.0	0.0	5.475177915906377E-4	0.0
62-63	0.08159840154005804	0.0	0.0	5.840189776966803E-4	0.0
64-65	0.10205731635249488	0.0	0.0	5.840189776966803E-4	0.0
66-67	0.12545457664646814	0.0	0.0	5.840189776966803E-4	0.0
68-69	0.1541080077397115	0.0	0.0	5.840189776966803E-4	0.0
70-71	0.1911384610442916	0.0	0.0	6.570213499087653E-4	0.0
72-73	0.232658560239915	0.0	0.0	6.570213499087653E-4	0.0
74-75	0.27801128397667285	0.0	0.0	6.570213499087653E-4	0.0
76-77	0.3272148828476181	0.0	0.0	6.570213499087653E-4	0.0
78-79	0.38784335296975475	0.0	0.0	7.117731290678291E-4	0.0
80-81	0.4616487512761727	0.0	0.0	7.665249082268929E-4	0.0
82-83	0.5538689979730891	0.0	0.0	7.665249082268929E-4	0.0
84-85	0.6668949207504498	0.0	0.0	7.665249082268929E-4	0.0
86-87	0.7957076065186738	0.0	0.0	8.030260943329353E-4	0.0
88	0.9012507861442958	0.0	0.0	8.030260943329353E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4730	0.0	87.192276	1
AACGTGT	2165	0.0	78.06774	1
CGTGTGG	2435	0.0	74.71442	3
GTGGAAT	77820	0.0	74.32777	6
TCGAGTG	1640	0.0	74.24312	2
TGGAATC	78260	0.0	73.78363	7
GGAATCA	78670	0.0	73.4575	8
GAATCAC	79045	0.0	73.12684	9
ACGAGTG	72975	0.0	72.66668	2
CGAGTGG	75250	0.0	72.50508	3
AACGAGT	67460	0.0	72.3645	1
AGTGGAA	77260	0.0	72.34763	5
ATCGAGT	1615	0.0	72.296	1
GAGTGGA	77660	0.0	72.02343	4
ACGTGTG	2475	0.0	70.279205	2
GCGAGTG	835	0.0	57.42672	2
AGCGAGT	805	0.0	57.3146	1
GTGTGGA	3675	0.0	54.745335	4
TGAGTGG	2965	0.0	54.22428	3
ATGAGTG	3075	0.0	48.004818	2
>>END_MODULE