FastQCFastQC Report
Thu 26 May 2016
SRR2132774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483128
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1897717.642417144827009No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1314565.293967930771188No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG201910.8131276357884089No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT155090.6245751326552638No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105790.4260352265368519No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC67860.27328434136299057No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62090.2500475207077525No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54780.22060884497295347No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA50560.20361415118350726No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50400.2029698026038126No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43840.17655151083633225No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGTCAAGTAATTAAGTGCATG37680.1517440905180885No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.12653395233753556No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA31350.12625204983391916No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30490.12278867621806046No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26520.10680077708438711No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.10369984954460665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT37200.085.670541
AACGTGT18500.074.3013841
CGTGTGG20800.072.546223
ACGTGTG20500.070.6325452
TCGAGTG14750.069.800752
ATCGAGT14350.069.217561
GTGGAAT686050.067.7813646
TGGAATC688900.067.484617
GGAATCA694350.066.8736958
GAATCAC696550.066.6827249
AACGAGT593500.065.896381
ACGAGTG645000.065.670922
CGAGTGG666950.065.4498143
AGTGGAA689500.065.1597755
GAGTGGA691450.065.01684
TGAGTGG24950.056.7112163
ATGAGTG24900.052.487212
GCGAGTG7550.051.059312
AGCGAGT6900.049.8034631
AATGAGT24600.048.7967381
Produced by FastQC (version 0.11.5)