##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132774_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2483128
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.936884848465322	31.0	25.0	33.0	16.0	34.0
2	28.24752852047901	31.0	25.0	34.0	16.0	34.0
3	29.302657776804097	31.0	28.0	34.0	16.0	34.0
4	34.752805735346705	35.0	35.0	37.0	32.0	37.0
5	33.87732005760476	35.0	35.0	37.0	28.0	37.0
6	35.251389376624964	37.0	35.0	37.0	32.0	37.0
7	35.61509636233009	37.0	35.0	37.0	33.0	37.0
8	35.88934360210187	37.0	35.0	37.0	35.0	37.0
9	37.85118930639097	39.0	38.0	39.0	35.0	39.0
10-11	37.85074551130671	39.0	38.0	39.0	35.0	39.0
12-13	37.81299997422606	39.0	38.0	39.0	35.0	39.0
14-15	39.21096878614392	41.0	39.0	41.0	36.0	41.0
16-17	39.11237781540058	40.5	39.0	41.0	36.0	41.0
18-19	38.79391638288482	40.0	38.0	41.0	35.5	41.0
20-21	38.62170093527196	40.0	38.0	41.0	35.0	41.0
22-23	38.42617658050652	40.0	38.0	41.0	35.0	41.0
24-25	38.22153851915809	40.0	38.0	41.0	34.5	41.0
26-27	37.94638717778544	40.0	38.0	41.0	34.0	41.0
28-29	37.747609064051474	40.0	38.0	41.0	33.5	41.0
30-31	37.60307060288475	40.0	37.5	41.0	33.0	41.0
32-33	37.43300526593877	40.0	37.0	41.0	33.0	41.0
34-35	37.32431775566946	40.0	37.0	41.0	33.0	41.0
36-37	37.12754638504338	40.0	36.5	41.0	33.0	41.0
38-39	36.61188247242994	39.0	35.5	41.0	32.5	41.0
40-41	36.35280420501883	39.0	35.0	41.0	31.0	41.0
42-43	36.17551068652119	39.0	35.0	41.0	31.0	41.0
44-45	36.02360087760277	39.0	35.0	41.0	31.0	41.0
46-47	35.87974099603403	39.0	35.0	41.0	30.5	41.0
48-49	35.70350541736069	39.0	35.0	41.0	30.0	41.0
50-51	35.40302795506313	38.5	35.0	41.0	29.0	41.0
52-53	34.88704045864732	38.0	35.0	41.0	25.5	41.0
54-55	33.954216415746586	38.0	34.5	40.0	16.0	41.0
56-57	33.171863874919055	38.0	34.0	40.0	2.0	41.0
58-59	32.80153540212184	37.0	34.0	40.0	2.0	41.0
60-61	32.55132739834596	37.0	33.5	40.0	2.0	41.0
62-63	32.29057241511513	36.0	33.0	40.0	2.0	41.0
64-65	32.02691645376316	36.0	33.0	40.0	2.0	41.0
66-67	31.726630886526994	35.0	33.0	39.0	2.0	41.0
68-69	31.433758146982356	35.0	33.0	39.0	2.0	41.0
70-71	31.137759914108337	35.0	33.0	38.5	2.0	40.5
72-73	30.852849309419412	35.0	33.0	37.5	2.0	40.0
74-75	30.557835721718735	35.0	32.0	37.0	2.0	39.5
76-77	30.254327404789443	35.0	32.0	36.5	2.0	39.0
78-79	29.941474221224198	35.0	32.0	36.0	2.0	39.0
80-81	29.654153752847215	35.0	31.5	36.0	2.0	38.5
82-83	29.373029098781856	35.0	31.0	35.0	2.0	37.0
84-85	29.117501796121665	35.0	31.0	35.0	2.0	37.0
86-87	28.916686534081208	35.0	31.0	35.0	2.0	36.5
88-89	28.69110633845698	35.0	31.0	35.0	2.0	36.0
90-91	28.499329474759257	35.0	31.0	35.0	2.0	36.0
92-93	28.342673434474584	35.0	30.5	35.0	2.0	36.0
94-95	28.14675864474163	35.0	30.0	35.0	2.0	35.0
96-97	27.94113412598948	34.0	30.0	35.0	2.0	35.0
98-99	27.771788848581306	34.0	30.0	35.0	2.0	35.0
100	27.66844157852515	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.15501900280113645
1101	2	2.208132413000307
1101	3	1.1303610753607103
1101	4	0.5906103417824156
1101	5	2.1911269563470768
1101	6	0.9543263466057752
1101	7	0.5381417356091163
1101	8	0.39155934907626744
1101	9	0.29870300896426016
1101	10-11	0.4597230532364307
1101	12-13	0.39771660740257886
1101	14-15	0.49087181668870983
1101	16-17	0.4333508878169354
1101	18-19	0.46316897026297
1101	20-21	0.4646543273672634
1101	22-23	0.44586325932860404
1101	24-25	0.49318294428836396
1101	26-27	0.4331244056336274
1101	28-29	0.49124752470711286
1101	30-31	0.5459982383492559
1101	32-33	0.5867270837484995
1101	34-35	0.581086695874852
1101	36-37	0.6488875174366626
1101	38-39	0.7259875842344812
1101	40-41	0.7427192740595103
1101	42-43	0.751868336516587
1101	44-45	0.8345468138786458
1101	46-47	0.8061198658434563
1101	48-49	0.7726490900776071
1101	50-51	0.8269811669541909
1101	52-53	0.9648178682467332
1101	54-55	0.9790695414672328
1101	56-57	0.9752116870017389
1101	58-59	0.8888073556546132
1101	60-61	0.9543593208550831
1101	62-63	0.9492844247701981
1101	64-65	0.9369446501868488
1101	66-67	0.8633559362243162
1101	68-69	0.8914844880574897
1101	70-71	0.8701299790854335
1101	72-73	0.9151407723981357
1101	74-75	0.9428896287546564
1101	76-77	0.8598544260378347
1101	78-79	0.8973271656004123
1101	80-81	0.9215415921012955
1101	82-83	0.9187457073111922
1101	84-85	0.8285393419584892
1101	86-87	0.8873040848372717
1101	88-89	0.9364911517640628
1101	90-91	0.8552074703093204
1101	92-93	0.8421599967822644
1101	94-95	0.8297065213751509
1101	96-97	0.805949073228792
1101	98-99	0.8620955354746123
1101	100	0.8875517237243216
1102	1	1.4982974062518153
1102	2	3.0365945914058727
1102	3	3.230325815257313
1102	4	1.453164874671117
1102	5	2.5976638627042163
1102	6	1.2155059114691085
1102	7	0.6915178465077219
1102	8	0.5131604745834579
1102	9	0.48723948586390264
1102	10-11	0.5241490703818741
1102	12-13	0.46066973786614085
1102	14-15	0.5346906794946307
1102	16-17	0.4949626382903958
1102	18-19	0.38402590811366366
1102	20-21	0.30981591955161036
1102	22-23	0.2508051006921619
1102	24-25	0.25575962474616887
1102	26-27	0.2918869520115166
1102	28-29	0.30941806509606806
1102	30-31	0.34347755163967975
1102	32-33	0.3483938071285806
1102	34-35	0.36755192037787054
1102	36-37	0.3453436423689027
1102	38-39	0.38876447285010585
1102	40-41	0.4016047907813629
1102	42-43	0.40623592514793216
1102	44-45	0.4236627943911273
1102	46-47	0.3978076044798513
1102	48-49	0.39497791723621845
1102	50-51	0.4797779209125892
1102	52-53	0.3887198475060458
1102	54-55	0.36983385847727135
1102	56-57	0.20787949810614492
1102	58-59	0.19526722953960274
1102	60-61	0.23583107522416924
1102	62-63	0.17357743329598208
1102	64-65	0.17693298091136356
1102	66-67	0.19755304430914578
1102	68-69	0.2127023872828211
1102	70-71	0.18860204909404388
1102	72-73	0.1717910860429157
1102	74-75	0.18403224384574557
1102	76-77	0.2341939004569511
1102	78-79	0.21712964448541783
1102	80-81	0.20798986124676944
1102	82-83	0.2261953868607982
1102	84-85	0.19630040166246587
1102	86-87	0.14921079399598014
1102	88-89	0.21003577779576332
1102	90-91	0.19768460111081154
1102	92-93	0.16792301720613523
1102	94-95	0.21208943553762793
1102	96-97	0.22529698425834255
1102	98-99	0.3070100429244107
1102	100	0.2888137538145088
1103	1	1.26402980023542
1103	2	2.8569974085062917
1103	3	3.1677926330268384
1103	4	1.3892220918065945
1103	5	2.54226249163041
1103	6	1.199714318212088
1103	7	0.6815891414647908
1103	8	0.47708401662521993
1103	9	0.3658264493283099
1103	10-11	0.43653569876116904
1103	12-13	0.407864296581792
1103	14-15	0.5626407841501404
1103	16-17	0.3739663262371167
1103	18-19	0.3105013705866355
1103	20-21	0.2004780946975373
1103	22-23	0.1557262390146974
1103	24-25	0.18159543010448687
1103	26-27	0.24536620611817028
1103	28-29	0.2628837653504803
1103	30-31	0.26572853250090844
1103	32-33	0.32661256890172297
1103	34-35	0.27619971861387427
1103	36-37	0.2975281365021445
1103	38-39	0.2702718679165983
1103	40-41	0.3633775058941282
1103	42-43	0.42523035016612454
1103	44-45	0.5278232171086756
1103	46-47	0.5037136504184829
1103	48-49	0.5216498202077986
1103	50-51	0.5086132615432035
1103	52-53	0.5458194146129429
1103	54-55	0.5960833443485924
1103	56-57	0.5856513103064529
1103	58-59	0.5191482757366117
1103	60-61	0.5757676759507788
1103	62-63	0.5281368051833581
1103	64-65	0.5649639862519855
1103	66-67	0.6135133152574284
1103	68-69	0.5816878865245698
1103	70-71	0.5478347045258083
1103	72-73	0.5266660367901927
1103	74-75	0.5669630475332745
1103	76-77	0.5832217475805663
1103	78-79	0.6041545021939712
1103	80-81	0.5845497049211552
1103	82-83	0.5982420236333148
1103	84-85	0.5457770789559184
1103	86-87	0.48525187639984324
1103	88-89	0.49516209659264376
1103	90-91	0.5308251026586923
1103	92-93	0.5987251234521835
1103	94-95	0.6381184115277101
1103	96-97	0.6328518054963013
1103	98-99	0.6166080113826773
1103	100	0.6307337349204367
1104	1	6.0354083302524835
1104	2	5.042058671980918
1104	3	4.0908929965053495
1104	4	1.8469063748324217
1104	5	2.8317757073668943
1104	6	1.2993070915417135
1104	7	0.7122340130176426
1104	8	0.5478857224120688
1104	9	0.4136616738560832
1104	10-11	0.4119658614022015
1104	12-13	0.36801923056606256
1104	14-15	0.544841252664483
1104	16-17	0.3163098453766935
1104	18-19	0.29546541350008226
1104	20-21	0.3003672449840664
1104	22-23	0.263986495128556
1104	24-25	0.2645089164769985
1104	26-27	0.3134999754321015
1104	28-29	0.3464096390125846
1104	30-31	0.42657031376040777
1104	32-33	0.3920053612338563
1104	34-35	0.47876190678785946
1104	36-37	0.4575059948402185
1104	38-39	0.5520810775144156
1104	40-41	0.5374005428597499
1104	42-43	0.6070350837893486
1104	44-45	0.6383903404933449
1104	46-47	0.538020472875175
1104	48-49	0.654528453259303
1104	50-51	0.5894814777019945
1104	52-53	0.5945653884792392
1104	54-55	0.6755839680753795
1104	56-57	0.5606285184589055
1104	58-59	0.6000393068570347
1104	60-61	0.5801105678012775
1104	62-63	0.6265055653389382
1104	64-65	0.6734913324172744
1104	66-67	0.5598384123080322
1104	68-69	0.5787104932418892
1104	70-71	0.5409260132654303
1104	72-73	0.4541524474757104
1104	74-75	0.5507395703340698
1104	76-77	0.5980801069145265
1104	78-79	0.6188476094160791
1104	80-81	0.58112686242929
1104	82-83	0.5638962335484727
1104	84-85	0.5845255311959932
1104	86-87	0.6586831384011518
1104	88-89	0.5984550270491056
1104	90-91	0.5660221437077304
1104	92-93	0.5970448110454711
1104	94-95	0.6159919776998279
1104	96-97	0.5457071362868682
1104	98-99	0.5515578513706636
1104	100	0.5197678527784504
1105	1	1.8204804555321168
1105	2	3.093454923532537
1105	3	3.277059088225986
1105	4	1.4660855060667117
1105	5	2.572980536380463
1105	6	1.257048425212517
1105	7	0.7547450855751308
1105	8	0.5601649789059451
1105	9	0.4028369382376198
1105	10-11	0.47874164822997756
1105	12-13	0.4215614148165301
1105	14-15	0.5000338164944793
1105	16-17	0.29473500322520607
1105	18-19	0.21334522037280124
1105	20-21	0.21765023239915138
1105	22-23	0.12435502314767177
1105	24-25	0.09640151754690152
1105	26-27	0.1117894714629557
1105	28-29	0.13378519101460995
1105	30-31	0.17477660369164028
1105	32-33	0.18583912771795497
1105	34-35	0.18203991473713899
1105	36-37	0.17423568221578023
1105	38-39	0.17679097027135526
1105	40-41	0.15742953945784421
1105	42-43	0.23504006389483578
1105	44-45	0.2309251024548189
1105	46-47	0.31471958791281907
1105	48-49	0.2598143940671349
1105	50-51	0.2549904251429638
1105	52-53	0.34309170392081256
1105	54-55	0.30200390636565544
1105	56-57	0.30629864553412034
1105	58-59	0.19148064610369175
1105	60-61	0.09804524304374951
1105	62-63	0.10384686898752449
1105	64-65	0.14452282685084938
1105	66-67	0.20192152970566113
1105	68-69	0.1431849056471215
1105	70-71	0.2860282130003675
1105	72-73	0.26056289401244825
1105	74-75	0.24370469157808117
1105	76-77	0.2272111599597082
1105	78-79	0.22675630947229308
1105	80-81	0.18442860242773662
1105	82-83	0.23603336321363244
1105	84-85	0.12833112520033296
1105	86-87	0.20768539550453724
1105	88-89	0.18141666311482751
1105	90-91	0.1965857820078476
1105	92-93	0.2879782620511051
1105	94-95	0.31138726184527243
1105	96-97	0.32904546799204226
1105	98-99	0.3213341858693113
1105	100	0.4166702766351946
1106	1	-0.6793474938947206
1106	2	1.921996657392416
1106	3	2.7601194957754984
1106	4	1.2138496576308597
1106	5	2.4520169021545897
1106	6	1.1314494573002136
1106	7	0.6608276935813606
1106	8	0.44635206853296694
1106	9	0.35180307070253747
1106	10-11	0.4536544094205439
1106	12-13	0.3721941186640976
1106	14-15	0.4227493462430445
1106	16-17	0.32118759007838094
1106	18-19	0.2422891426728171
1106	20-21	0.3238027393216285
1106	22-23	0.26468400110255175
1106	24-25	0.30088507997536595
1106	26-27	0.21245151559634934
1106	28-29	0.31196157359467946
1106	30-31	0.3167265785042943
1106	32-33	0.33877445072537427
1106	34-35	0.38115129240379275
1106	36-37	0.34938170946556824
1106	38-39	0.40356915878916055
1106	40-41	0.3965221803316652
1106	42-43	0.4410787154066682
1106	44-45	0.5235010932557742
1106	46-47	0.45654545978852923
1106	48-49	0.4635086812330229
1106	50-51	0.48498542003538603
1106	52-53	0.338037462146076
1106	54-55	0.31149699899358296
1106	56-57	0.38135293045633745
1106	58-59	0.475274668667673
1106	60-61	0.49869854981262307
1106	62-63	0.4623029119537918
1106	64-65	0.47532327731054025
1106	66-67	0.5218532380330174
1106	68-69	0.4766204265936054
1106	70-71	0.500205665637921
1106	72-73	0.5377261198474308
1106	74-75	0.4429307876049542
1106	76-77	0.45861594479941203
1106	78-79	0.44766072378302013
1106	80-81	0.45258565493714187
1106	82-83	0.4689389252473042
1106	84-85	0.4263219088987853
1106	86-87	0.40779833403124144
1106	88-89	0.5049083081448309
1106	90-91	0.5358888207769326
1106	92-93	0.4754709404632358
1106	94-95	0.43957378408294545
1106	96-97	0.5388930171612927
1106	98-99	0.5307033373633452
1106	100	0.47656059574316245
1107	1	-0.8162301201426523
1107	2	1.6851355119322484
1107	3	-0.6599079859942449
1107	4	-0.10207647119021601
1107	5	1.7850684469528275
1107	6	0.7079787984651347
1107	7	0.25119964080909085
1107	8	0.22783926643808172
1107	9	0.21615155499996774
1107	10-11	0.24654455280764154
1107	12-13	0.24434359539665707
1107	14-15	0.3219247226682782
1107	16-17	0.24909721392592132
1107	18-19	0.1779617413707868
1107	20-21	0.1643078901644941
1107	22-23	0.16894299348311392
1107	24-25	-0.05401583562900214
1107	26-27	-0.03440234458076219
1107	28-29	-0.031778367941207364
1107	30-31	0.030905319116392604
1107	32-33	0.01973241430123096
1107	34-35	0.11073704279930752
1107	36-37	0.10214773026897461
1107	38-39	0.09306289412767654
1107	40-41	0.15557326369075497
1107	42-43	0.11971277598021146
1107	44-45	0.21426878706021313
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2216	90-91	0.2512239916043093
2216	92-93	0.2699566827008546
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2216	98-99	0.044485917369947
2216	100	0.014216622357043462
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1420.0
3	369.0
4	539.0
5	1682.0
6	5056.0
7	8891.0
8	10860.0
9	14428.0
10	9892.0
11	7194.0
12	6114.0
13	8628.0
14	8651.0
15	9381.0
16	10812.0
17	12568.0
18	17408.0
19	29945.0
20	59461.0
21	69146.0
22	29736.0
23	17258.0
24	17041.0
25	18029.0
26	19750.0
27	21943.0
28	26260.0
29	31011.0
30	35962.0
31	44088.0
32	56861.0
33	81284.0
34	117701.0
35	268996.0
36	301193.0
37	475208.0
38	487411.0
39	140477.0
40	474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.788692136056659	44.46081699002444	24.398762622688235	18.35172825123066
2	13.901335719688152	44.27294980358485	24.026925428291353	17.798789048435644
3	17.05947003059545	26.751787822149304	18.884524630806723	37.30421751644853
4	33.39457329626181	28.398374952882012	20.67094406732154	17.536107683534638
5	15.981092630725161	43.0319046754137	21.348517329271665	19.638485364589478
6	37.12180742209794	25.12400169322978	22.75585632357283	14.998334561099455
7	17.757514078514273	24.749188393738482	40.736573207267384	16.75672432047986
8	36.37657825130239	25.11179447857702	22.210776085646813	16.30085118447378
9	35.65929746674356	26.8502872183794	22.293292975633957	15.197122339243082
10-11	15.3692238177009	46.30172105505637	22.367896459626728	15.961158667616008
12-13	15.230809688425245	26.504352574655837	32.01443904623523	26.250398690683685
14-15	15.511665125599647	35.98779442702914	22.29661539799801	26.20392504937321
16-17	25.740497469320957	25.950917552377483	32.140187698741265	16.168397279560295
18-19	15.809535392456612	26.178936406016927	32.39736332561189	25.614164875914575
20-21	25.78834035136328	36.31772103572591	22.35525111875022	15.538687494160591
22-23	25.2614444362111	26.472960717288842	32.41655283175092	15.84904201474914
24-25	15.582986459014597	36.27779155967795	22.097149240796284	26.042072740511163
26-27	16.220981895203487	30.575023060645524	37.14333522677963	16.060659817371366
28-29	25.143609189699447	26.297295185749586	32.54965108524409	16.009444539306873
30-31	22.558120241888457	27.780867518710274	32.84981684391622	16.811195395485047
32-33	18.19904169257485	29.445401123099575	34.00442909104967	18.3511280932759
34-35	18.243743375291167	29.533797693876433	33.97644019961919	18.246018731213212
36-37	18.58744293487891	29.171714063874276	34.606411751629395	17.634431249617418
38-39	19.318979931763486	28.838102586737374	33.92201288052811	17.92090460097103
40-41	17.736359140567863	30.376565364330794	34.13271486608826	17.75436062901308
42-43	17.673422852901627	29.39490726738719	34.638297906614575	18.29337197309661
44-45	17.99295082653814	29.946261328453467	33.60644316362266	18.454344681385738
46-47	18.935894565241902	28.759894777876937	34.16056280626694	18.143647850614226
48-49	17.88572719569833	29.360246431114305	34.20729821418791	18.546728158999457
50-51	18.582006243737737	30.162379869261674	33.143599524470744	18.11201436252984
52-53	18.991005488848632	29.5378344751403	33.08109074631951	18.39006928969156
54-55	19.094504995312363	30.5136102528746	30.951223617952838	19.440661133860196
56-57	19.46099838590681	31.404099990012597	29.532911714579352	19.601989909501242
58-59	20.56676095634216	31.543037652509256	28.294393200833788	19.595808190314795
60-61	20.11996562400327	31.95328633884359	27.841939682529453	20.084808354623686
62-63	18.98863046931129	32.1722843123673	27.825790696250856	21.01329452207055
64-65	19.906202982689575	32.02831267659178	28.468890850572343	19.5965934901463
66-67	20.00126453408765	31.448741265049566	28.75651194783354	19.793482253029243
68-69	20.41304354829876	31.697379273239235	28.440418697707088	19.449158480754917
70-71	18.386204819083027	33.015635923722016	28.684264363335277	19.91389489385968
72-73	18.631344924815245	32.088788423740894	29.043502389034103	20.236364262409758
74-75	19.043577469939006	32.384103373258945	28.12170021722689	20.450618939575165
76-77	20.029100550941166	31.498446809440324	28.405717899839093	20.066734739779417
78-79	19.540972370025838	32.12245005624983	27.86403468058411	20.47254289314022
80-81	19.920136377475327	32.81536687087311	27.570689635730705	19.69380711592085
82-83	19.60033119810431	33.00322257688556	27.411556333076398	19.984889891933733
84-85	19.663118750127108	33.18176207527645	26.709289454856943	20.445829719739493
86-87	19.562467090393064	33.21120240503156	27.13499407501226	20.09133642956312
88-89	20.98097641362024	32.33681469501371	26.924085266647552	19.7581236247185
90-91	19.838208066239048	33.3328634957327	26.981155820633457	19.8477726173948
92-93	19.10213649880312	33.39608348824547	27.033966835378603	20.4678131775728
94-95	19.286581279740712	33.58155519973195	26.736237519773447	20.39562600075389
96-97	19.396442624314137	32.49662870261281	27.383855104990968	20.723073568082086
98-99	20.415904938775505	32.70687569708296	26.087409143875913	20.789810220265633
100	19.29973001794511	34.018463808551154	25.8348744003531	20.84693177315064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144332.0
1	77385.0
2	7366.5
3	3484.5
4	2495.5
5	2153.5
6	2145.5
7	2489.5
8	2384.5
9	2085.0
10	2054.0
11	3099.0
12	20531.0
13	22623.5
14	7214.5
15	6291.5
16	7005.5
17	8006.0
18	9066.0
19	10239.5
20	11529.0
21	13130.5
22	14981.5
23	16963.5
24	18972.0
25	21601.5
26	25071.0
27	28507.0
28	31452.5
29	34074.5
30	36793.0
31	39787.5
32	43296.5
33	47508.0
34	52097.5
35	56264.0
36	59673.5
37	62549.0
38	64854.0
39	65300.0
40	65162.0
41	65195.0
42	64435.0
43	64743.0
44	66792.0
45	69131.0
46	71509.5
47	73974.5
48	75371.0
49	82212.0
50	107638.5
51	109751.0
52	81720.0
53	67557.5
54	64069.5
55	64890.0
56	58474.5
57	46091.5
58	38486.5
59	30665.5
60	23230.5
61	18677.5
62	14775.0
63	11081.0
64	8154.0
65	6121.0
66	4691.0
67	3555.0
68	2642.0
69	1984.5
70	1545.5
71	1197.0
72	945.5
73	690.5
74	469.0
75	317.0
76	200.0
77	125.5
78	70.0
79	38.0
80	17.5
81	15.0
82	10.5
83	5.5
84	3.0
85	1.0
86	1.5
87	1.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13559510423949148
2	0.004913157920171655
3	0.003181471112242301
4	0.0
5	0.0030203839673186403
6	0.012041264083043644
7	0.003584188974551453
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.04076793463728E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.457075108492192E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.0067946557728802E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.631255416555248E-4
74-75	4.0271786230915206E-4
76-77	5.436691141173552E-4
78-79	3.825819691936944E-4
80-81	8.054357246183041E-4
82-83	8.859792970801344E-4
84-85	0.001107474121350168
86-87	2.01358931154576E-5
88-89	0.0
90-91	2.01358931154576E-5
92-93	0.0
94-95	0.0
96-97	0.0013491048387356593
98-99	5.033973278864399E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2483128.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.29505880290197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46802214711838	53.050116341927186
2	7.165157575445172	8.497168795361599
3	1.4597838883857566	2.5967391450408694
4	0.5303850254745912	1.2579684509477815
5	0.27986625433633694	0.8297343003910508
6	0.1762453629632559	0.6270287496387036
7	0.11393130703283906	0.47288944850025694
8	0.07803348540938268	0.37016000847557906
9	0.06010638619299933	0.3207610533369447
>10	0.5867202862686138	7.451432119279598
>50	0.047062464997711835	1.9155440842918445
>100	0.028975866287234916	3.1762331471010463
>500	0.0029229506401774524	1.2044692717671672
>1k	0.0021072434847790515	2.265488885259718
>5k	3.3987798141597603E-4	1.1515069646660536
>10k+	3.3987798141597603E-4	14.812759234014589
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	189771	7.642417144827009	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	131456	5.293967930771188	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	20191	0.8131276357884089	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15509	0.6245751326552638	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10579	0.4260352265368519	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6786	0.27328434136299057	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6209	0.2500475207077525	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5478	0.22060884497295347	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	5056	0.20361415118350726	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5040	0.2029698026038126	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4384	0.17655151083633225	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGTCAAGTAATTAAGTGCATG	3768	0.1517440905180885	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3142	0.12653395233753556	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	3135	0.12625204983391916	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3049	0.12278867621806046	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2652	0.10680077708438711	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2575	0.10369984954460665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001610871449236608	0.0	0.0	0.0	0.0
2	0.001610871449236608	0.0	0.0	0.0	0.0
3	0.001610871449236608	0.0	0.0	0.0	0.0
4	0.001610871449236608	4.02717862309152E-5	0.0	0.0	0.0
5	0.001610871449236608	8.05435724618304E-5	0.0	0.0	0.0
6	0.001610871449236608	8.05435724618304E-5	0.0	0.0	0.0
7	0.001610871449236608	8.05435724618304E-5	0.0	0.0	0.0
8	0.001610871449236608	8.05435724618304E-5	0.0	0.0	0.0
9	0.001610871449236608	8.05435724618304E-5	0.0	0.0	0.0
10-11	0.001610871449236608	1.812230380391184E-4	0.0	0.0	0.0
12-13	0.0016310073423520656	2.819025036164064E-4	0.0	0.0	0.0
14-15	0.0016712791285829808	3.221742898473216E-4	0.0	0.0	0.0
16-17	0.0017115509148138961	4.832614347709824E-4	0.0	0.0	0.0
18-19	0.0017920944872757264	5.638050072328128E-4	0.0	0.0	0.0
20-21	0.001812230380391184	6.644844728101009E-4	0.0	0.0	0.0
22-23	0.0018927739528530143	7.248921521564736E-4	0.0	0.0	0.0
24-25	0.0019330457390839297	7.248921521564736E-4	0.0	0.0	0.0
26-27	0.0020337252046612178	7.450280452719312E-4	0.0	0.0	0.0
28-29	0.0021344046702385057	7.651639383873889E-4	0.0	0.0	0.0
30-31	0.002255220028931251	8.859792970801344E-4	0.0	0.0	0.0
32-33	0.0024364430669703697	9.262510833110496E-4	0.0	0.0	0.0
34-35	0.002778753249933149	9.262510833110496E-4	0.0	0.0	0.0
36-37	0.0034231018296277923	9.262510833110496E-4	0.0	0.0	0.0
38-39	0.004490304164747045	9.463869764265072E-4	0.0	0.0	0.0
40-41	0.006805931873024669	9.665228695419648E-4	0.0	0.0	0.0
42-43	0.009282646726225953	0.0010269305488883377	0.0	0.0	0.0
44-45	0.012484253731583711	0.0011276100144656256	0.0	0.0	0.0
46-47	0.015484501805786895	0.001208153586927456	0.0	0.0	0.0
48-49	0.018786788276721943	0.0012685612662738288	0.0	0.0	0.0
50-51	0.02378048976935543	0.0013491048387356593	0.0	0.0	0.0
52-53	0.029660170559069048	0.0014900560905438624	0.0	0.0	0.0
54-55	0.03783534316394483	0.0015907355561211505	0.0	0.0	0.0
56-57	0.046675000241630724	0.001671279128582981	0.0	0.0	0.0
58-59	0.05571601625047118	0.0017920944872757264	0.0	0.0	0.0
60-61	0.0680995905164776	0.0018726380597375567	0.0	0.0	0.0
62-63	0.08330218981864809	0.0018927739528530145	0.0	0.0	0.0
64-65	0.10297495739245016	0.001953181632199387	0.0	0.0	0.0
66-67	0.12468145017091346	0.0019733175253148446	0.0	0.0	0.0
68-69	0.15321401071551688	0.0020337252046612178	0.0	0.0	0.0
70-71	0.19187492549719548	0.002214948242700336	0.0	0.0	0.0
72-73	0.23514695980231387	0.002214948242700336	0.0	0.0	0.0
74-75	0.28077489360194074	0.0022753559220467087	0.0	0.0	0.0
76-77	0.3311347622837002	0.002355899494508539	0.0	2.01358931154576E-5	0.0
78-79	0.3912404032333412	0.002376035387623997	0.0	4.02717862309152E-5	0.0
80-81	0.46528008221887873	0.0024364430669703697	0.0	4.02717862309152E-5	0.0
82-83	0.5572407060771736	0.002476714853201285	0.0	4.02717862309152E-5	0.0
84-85	0.6674645849911885	0.0024968507463167425	0.0	4.02717862309152E-5	0.0
86-87	0.796153077892078	0.0025169866394322	0.0	4.02717862309152E-5	0.0
88	0.90514866732605	0.0025773943187785728	0.0	4.02717862309152E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3720	0.0	85.67054	1
AACGTGT	1850	0.0	74.301384	1
CGTGTGG	2080	0.0	72.54622	3
ACGTGTG	2050	0.0	70.632545	2
TCGAGTG	1475	0.0	69.80075	2
ATCGAGT	1435	0.0	69.21756	1
GTGGAAT	68605	0.0	67.781364	6
TGGAATC	68890	0.0	67.48461	7
GGAATCA	69435	0.0	66.873695	8
GAATCAC	69655	0.0	66.682724	9
AACGAGT	59350	0.0	65.89638	1
ACGAGTG	64500	0.0	65.67092	2
CGAGTGG	66695	0.0	65.449814	3
AGTGGAA	68950	0.0	65.159775	5
GAGTGGA	69145	0.0	65.0168	4
TGAGTGG	2495	0.0	56.711216	3
ATGAGTG	2490	0.0	52.48721	2
GCGAGTG	755	0.0	51.05931	2
AGCGAGT	690	0.0	49.803463	1
AATGAGT	2460	0.0	48.796738	1
>>END_MODULE