FastQCFastQC Report
Thu 26 May 2016
SRR2132773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292618
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1468956.407303789815836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA505582.205251812556649No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG133540.5824781974144843No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT123070.5368098828500867No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49540.2160848427430998No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41300.18014339938009735No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41190.17966359855850383No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39330.1715506028479232No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC35480.15475757409215143No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33630.14668819663807925No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA29350.12801958285244205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT32500.085.066251
GTGGAAT519750.070.0523456
TGGAATC523200.069.510967
GAATCAC524950.069.303089
AACGAGT442200.069.081351
GGAATCA527200.068.971648
ACGAGTG485150.068.808172
CGAGTGG502300.068.1577453
AGTGGAA519500.067.6822365
GAGTGGA522400.067.288514
AACGTGT15600.066.118361
CGTGTGG17950.063.637033
ATCGAGT9650.061.495851
ACGTGTG18300.058.8275682
TCGAGTG10950.056.670412
GTGTGGA28200.044.5062264
TGAGTGG23800.042.6623573
AATGAGT19550.042.641171
ATGAGTG22200.041.504922
AGTACTT246150.041.4031120-21
Produced by FastQC (version 0.11.5)