##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132773_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2292618
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.900870533163395	31.0	25.0	33.0	16.0	34.0
2	28.209630649327536	31.0	25.0	34.0	16.0	34.0
3	29.294020634924788	31.0	28.0	34.0	16.0	34.0
4	34.78417686679595	35.0	35.0	37.0	32.0	37.0
5	33.89671763896122	35.0	35.0	37.0	28.0	37.0
6	35.306715292299025	37.0	35.0	37.0	32.0	37.0
7	35.693092351189776	37.0	35.0	37.0	33.0	37.0
8	35.96789042047127	37.0	35.0	37.0	35.0	37.0
9	37.944764020870466	39.0	38.0	39.0	35.0	39.0
10-11	37.93629030217856	39.0	38.0	39.0	35.0	39.0
12-13	37.91571273539682	39.0	38.0	39.0	35.0	39.0
14-15	39.34596561660076	41.0	39.0	41.0	36.0	41.0
16-17	39.32289875592009	41.0	39.0	41.0	36.0	41.0
18-19	39.19625532906049	41.0	39.0	41.0	35.5	41.0
20-21	39.12604476628902	40.5	39.0	41.0	35.0	41.0
22-23	38.9876734807107	40.0	39.0	41.0	35.0	41.0
24-25	38.85692884728289	40.0	39.0	41.0	35.0	41.0
26-27	38.69752832787669	40.0	39.0	41.0	35.0	41.0
28-29	38.56176824922425	40.0	39.0	41.0	35.0	41.0
30-31	38.449245796726714	40.0	38.0	41.0	35.0	41.0
32-33	38.307695612614054	40.0	38.0	41.0	34.5	41.0
34-35	38.218239148432055	40.0	38.0	41.0	34.0	41.0
36-37	38.06031009090917	40.0	38.0	41.0	34.0	41.0
38-39	37.62463916797303	40.0	36.0	41.0	33.5	41.0
40-41	37.389985161069134	40.0	35.0	41.0	33.0	41.0
42-43	37.239279941097905	40.0	35.0	41.0	33.0	41.0
44-45	37.10523231519599	40.0	35.0	41.0	33.0	41.0
46-47	36.975659268137996	40.0	35.0	41.0	33.0	41.0
48-49	36.81659766258487	40.0	35.0	41.0	33.0	41.0
50-51	36.547045561013654	39.0	35.0	41.0	31.5	41.0
52-53	36.13046351376461	39.0	35.0	41.0	30.5	41.0
54-55	35.33931557721347	39.0	35.0	41.0	27.0	41.0
56-57	34.69125384167794	39.0	35.0	41.0	22.5	41.0
58-59	34.36044317020978	38.5	35.0	41.0	20.0	41.0
60-61	34.10819726618216	38.0	35.0	40.0	19.5	41.0
62-63	33.8366055749366	37.0	34.5	40.0	18.0	41.0
64-65	33.55614825496441	37.0	34.0	40.0	17.0	41.0
66-67	33.246629617319584	36.0	34.0	40.0	14.0	41.0
68-69	32.94614017686331	36.0	34.0	39.0	11.0	41.0
70-71	32.62513532564082	35.0	34.0	39.0	7.5	41.0
72-73	32.31184698017725	35.0	33.5	38.5	6.0	40.0
74-75	31.989887979593632	35.0	33.0	37.0	2.0	40.0
76-77	31.676898419187147	35.0	33.0	37.0	2.0	39.0
78-79	31.354595924833532	35.0	33.0	36.5	2.0	39.0
80-81	31.05876862172416	35.0	33.0	36.0	2.0	38.5
82-83	30.775345696491957	35.0	33.0	36.0	2.0	37.0
84-85	30.503370818862976	35.0	33.0	35.0	2.0	37.0
86-87	30.312227113282717	35.0	33.0	35.0	2.0	36.5
88-89	30.114626161008943	35.0	32.5	35.0	2.0	36.0
90-91	29.931808090139743	35.0	32.0	35.0	2.0	36.0
92-93	29.779029476345382	35.0	32.0	35.0	2.0	36.0
94-95	29.60374733165316	35.0	32.0	35.0	2.0	35.0
96-97	29.426635401100402	35.0	32.0	35.0	2.0	35.0
98-99	29.28101825075089	35.0	32.0	35.0	2.0	35.0
100	29.1857313342214	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.07402594462635648
1101	2	2.1425580435458578
1101	3	1.1201683992437026
1101	4	0.6085292494698038
1101	5	2.2151199558357817
1101	6	0.9648474787288208
1101	7	0.49361609557343655
1101	8	0.3129537434572853
1101	9	0.2792617930798045
1101	10-11	0.39926562928613407
1101	12-13	0.38628719159773794
1101	14-15	0.5126366549252452
1101	16-17	0.4244001655917913
1101	18-19	0.3705771972299914
1101	20-21	0.4008987343464341
1101	22-23	0.3672190191357174
1101	24-25	0.34486590958153585
1101	26-27	0.22860851045899722
1101	28-29	0.3113946766980078
1101	30-31	0.37754209558595164
1101	32-33	0.3779830242397466
1101	34-35	0.30377329601329706
1101	36-37	0.3261574266824354
1101	38-39	0.37429762759400376
1101	40-41	0.3922527908567872
1101	42-43	0.4415236614501623
1101	44-45	0.5257898150690323
1101	46-47	0.4943576374586769
1101	48-49	0.3866118657641877
1101	50-51	0.4596821783456164
1101	52-53	0.555677760624036
1101	54-55	0.6163680680711607
1101	56-57	0.42395957210253243
1101	58-59	0.39874702543036733
1101	60-61	0.3738438953152965
1101	62-63	0.3866585908292066
1101	64-65	0.42501161327849246
1101	66-67	0.377845377787402
1101	68-69	0.4757542746307166
1101	70-71	0.45456611139633907
1101	72-73	0.4557989914873417
1101	74-75	0.45739004088074253
1101	76-77	0.4599656374937915
1101	78-79	0.45628431907951494
1101	80-81	0.43199549246907765
1101	82-83	0.48260844296030925
1101	84-85	0.5229191203525723
1101	86-87	0.5584079125266896
1101	88-89	0.5429095522371803
1101	90-91	0.5219234385584706
1101	92-93	0.5865495178737241
1101	94-95	0.6644674647639555
1101	96-97	0.5874551050869563
1101	98-99	0.5553966250978277
1101	100	0.5646086113469444
1102	1	1.6096582989338692
1102	2	3.1431681129011118
1102	3	3.2538815286467226
1102	4	1.428937538215017
1102	5	2.6830134334907143
1102	6	1.2572005018854995
1102	7	0.7647364507116805
1102	8	0.5943222390112268
1102	9	0.4634950868804637
1102	10-11	0.5426747282690272
1102	12-13	0.48588326829841577
1102	14-15	0.6207899278482785
1102	16-17	0.557763376155215
1102	18-19	0.5113933989778019
1102	20-21	0.47988174258270533
1102	22-23	0.4336112001370793
1102	24-25	0.5036074246303741
1102	26-27	0.5934300532171406
1102	28-29	0.633640141826362
1102	30-31	0.6512241271647241
1102	32-33	0.6355316931544905
1102	34-35	0.6602520891007231
1102	36-37	0.6444296187237981
1102	38-39	0.7327694960868243
1102	40-41	0.7107140155863902
1102	42-43	0.7309479418005935
1102	44-45	0.8410942212317565
1102	46-47	0.8631474320162908
1102	48-49	0.8599778636545636
1102	50-51	0.9277956020575715
1102	52-53	0.8909226218315496
1102	54-55	1.0959219317982019
1102	56-57	1.004176860634601
1102	58-59	0.9408780530249032
1102	60-61	0.9162122556018986
1102	62-63	0.9036324213815874
1102	64-65	0.9358541784937415
1102	66-67	0.9414230075757288
1102	68-69	0.9852564635021679
1102	70-71	0.9913016459892532
1102	72-73	0.9502134752033413
1102	74-75	1.0045894604608954
1102	76-77	0.9782227998309914
1102	78-79	0.9414558763208305
1102	80-81	0.8761472979464493
1102	82-83	0.8836295747243597
1102	84-85	0.7975027292304837
1102	86-87	0.7835442046166889
1102	88-89	0.8077259712325429
1102	90-91	0.8467780261707176
1102	92-93	0.7714627349955627
1102	94-95	0.7920015753607181
1102	96-97	0.8403723706645643
1102	98-99	0.8518275827359254
1102	100	0.8638711310905087
1103	1	1.2329181979040058
1103	2	2.957975892398064
1103	3	3.186169337674933
1103	4	1.376442172930041
1103	5	2.617854783158947
1103	6	1.2430628404638782
1103	7	0.7155246918230063
1103	8	0.506673800092436
1103	9	0.3844876476833292
1103	10-11	0.454614447192057
1103	12-13	0.42037800402673753
1103	14-15	0.5437295294333921
1103	16-17	0.47194954298932146
1103	18-19	0.387124764991853
1103	20-21	0.3480853614270316
1103	22-23	0.38271599667373835
1103	24-25	0.39584080105827013
1103	26-27	0.4950453940990158
1103	28-29	0.5120278433820502
1103	30-31	0.5759126003082855
1103	32-33	0.5482914854869279
1103	34-35	0.5545801890925048
1103	36-37	0.5325188996901957
1103	38-39	0.542289748338824
1103	40-41	0.5660588634946606
1103	42-43	0.6401788510846131
1103	44-45	0.6755357938089048
1103	46-47	0.6967489221216425
1103	48-49	0.7763311047019528
1103	50-51	0.7526559146190053
1103	52-53	0.8422136734541965
1103	54-55	0.7807502126334782
1103	56-57	0.749529010525535
1103	58-59	0.8247621843874455
1103	60-61	0.8631610425392466
1103	62-63	0.8525277853149547
1103	64-65	0.8139412803274837
1103	66-67	0.8048632107246902
1103	68-69	0.8434937803902613
1103	70-71	0.8226881925185054
1103	72-73	0.7943318146287268
1103	74-75	0.8480250734514421
1103	76-77	0.829526612200052
1103	78-79	0.8143365803694991
1103	80-81	0.817073887148009
1103	82-83	0.8268954684625918
1103	84-85	0.7848321892422057
1103	86-87	0.7993910838754488
1103	88-89	0.8318806529456673
1103	90-91	0.8409624451933446
1103	92-93	0.8623333617648647
1103	94-95	0.897962016649327
1103	96-97	0.9042646858661278
1103	98-99	0.860620936228937
1103	100	0.842816495815633
1104	1	6.116766106693767
1104	2	5.108825927117639
1104	3	4.154947550908986
1104	4	1.8631144240806492
1104	5	2.9128764401066363
1104	6	1.3481397878745014
1104	7	0.7229387241304721
1104	8	0.5599409586059707
1104	9	0.4407429674387302
1104	10-11	0.4788328940837019
1104	12-13	0.3923443166856444
1104	14-15	0.5119447981827534
1104	16-17	0.39525007479625174
1104	18-19	0.32064982053871915
1104	20-21	0.309354265526558
1104	22-23	0.2689148094767546
1104	24-25	0.2961056229195549
1104	26-27	0.3369227312443499
1104	28-29	0.25469391359563076
1104	30-31	0.33160506465249284
1104	32-33	0.39414318651708413
1104	34-35	0.3860951294533379
1104	36-37	0.4291150388123057
1104	38-39	0.4375269440558114
1104	40-41	0.43141905952759885
1104	42-43	0.4573184110539188
1104	44-45	0.5333283375684843
1104	46-47	0.4647746933740322
1104	48-49	0.5877475972193267
1104	50-51	0.5928820518616646
1104	52-53	0.6135419399478437
1104	54-55	0.685058303089896
1104	56-57	0.6076768620919353
1104	58-59	0.6029660657267897
1104	60-61	0.506214537916776
1104	62-63	0.5019744989420261
1104	64-65	0.546403409140737
1104	66-67	0.6059089399114939
1104	68-69	0.5560020467286648
1104	70-71	0.5284564902184101
1104	72-73	0.5546452846152192
1104	74-75	0.5317465996774153
1104	76-77	0.5426994622426626
1104	78-79	0.5923482538326894
1104	80-81	0.6228010010815304
1104	82-83	0.567649353434863
1104	84-85	0.6170700246341347
1104	86-87	0.5613242599219603
1104	88-89	0.5451954479640193
1104	90-91	0.5398676937726528
1104	92-93	0.5305868621305088
1104	94-95	0.5462228757580867
1104	96-97	0.5214837502915906
1104	98-99	0.52611093143042
1104	100	0.4993737238179463
1105	1	1.804428373167081
1105	2	3.2620024879903013
1105	3	3.3751544297616505
1105	4	1.4218217489325227
1105	5	2.6639406091777147
1105	6	1.2283871305663467
1105	7	0.7127193622830887
1105	8	0.4975335844201041
1105	9	0.37313671248664093
1105	10-11	0.4300240109753588
1105	12-13	0.3831856979377264
1105	14-15	0.46810893087624095
1105	16-17	0.3788005524389888
1105	18-19	0.3161029333142409
1105	20-21	0.2719792200545754
1105	22-23	0.20119306813636229
1105	24-25	0.16693366156950162
1105	26-27	0.19292185668723505
1105	28-29	0.23086061265330926
1105	30-31	0.23878484220104212
1105	32-33	0.25680582673275865
1105	34-35	0.2601925416612687
1105	36-37	0.24607602758508307
1105	38-39	0.21800195813344203
1105	40-41	0.2434630315617099
1105	42-43	0.19506707430208792
1105	44-45	0.26706157522377794
1105	46-47	0.3383212093477823
1105	48-49	0.2729513241781092
1105	50-51	0.356672023606734
1105	52-53	0.4093662411742187
1105	54-55	0.5059312531130686
1105	56-57	0.45373606038965875
1105	58-59	0.3739847752691787
1105	60-61	0.4424595733724672
1105	62-63	0.476129093730556
1105	64-65	0.42472685075968286
1105	66-67	0.4869980749720142
1105	68-69	0.5104953019249336
1105	70-71	0.4981965648672144
1105	72-73	0.5085257269268411
1105	74-75	0.535468699810469
1105	76-77	0.4544329166685195
1105	78-79	0.5208179532939994
1105	80-81	0.4285675964879907
1105	82-83	0.5246995669663299
1105	84-85	0.46489204104889836
1105	86-87	0.5193417186732603
1105	88-89	0.4861209212464246
1105	90-91	0.42458972912369575
1105	92-93	0.4896214607063065
1105	94-95	0.53514901430189
1105	96-97	0.5025313999504881
1105	98-99	0.42418154880296655
1105	100	0.5003946633563991
1106	1	-0.6703576598792758
1106	2	1.9342038508049306
1106	3	2.689786672084754
1106	4	1.16826016311186
1106	5	2.4649165766117562
1106	6	1.085779894248219
1106	7	0.5608407513932079
1106	8	0.39877977599796566
1106	9	0.2624282511524001
1106	10-11	0.40587545406710035
1106	12-13	0.36678625292439193
1106	14-15	0.3873081192556569
1106	16-17	0.30737888899604116
1106	18-19	0.2953034175053304
1106	20-21	0.2866308995529394
1106	22-23	0.27443898158878
1106	24-25	0.291072104825588
1106	26-27	0.2390121399959142
1106	28-29	0.2987225865853631
1106	30-31	0.2676234472755681
1106	32-33	0.27884347480294736
1106	34-35	0.38119037986811577
1106	36-37	0.36052069326566993
1106	38-39	0.3689550118242906
1106	40-41	0.39737638284677246
1106	42-43	0.37450238485441645
1106	44-45	0.42743249341697265
1106	46-47	0.405856385894225
1106	48-49	0.5367370222097421
1106	50-51	0.47176759761971
1106	52-53	0.5737472224513169
1106	54-55	0.6776321481712841
1106	56-57	0.6090503105255607
1106	58-59	0.6562329453302382
1106	60-61	0.6560169241012801
1106	62-63	0.6912189788141845
1106	64-65	0.6412506620895044
1106	66-67	0.6340562663981615
1106	68-69	0.6114551507258312
1106	70-71	0.6845880137743094
1106	72-73	0.6907958625762021
1106	74-75	0.6968456103425673
1106	76-77	0.6603489291083129
1106	78-79	0.6708337558755098
1106	80-81	0.658403502481594
1106	82-83	0.5441697696186338
1106	84-85	0.6013765671610862
1106	86-87	0.6019154219134215
1106	88-89	0.6095240703848681
1106	90-91	0.5970094836147908
1106	92-93	0.6346487043568274
1106	94-95	0.6464058252145186
1106	96-97	0.7011284467640557
1106	98-99	0.6986929329826843
1106	100	0.7359634298700968
1107	1	-1.1260459490864285
1107	2	1.6926970427515755
1107	3	-0.7838524379624552
1107	4	-0.07392262892067691
1107	5	1.882114404317356
1107	6	0.7679537659431475
1107	7	0.3154363655163692
1107	8	0.2502279225592403
1107	9	0.22818641523292627
1107	10-11	0.26183798069639863
1107	12-13	0.17509074924507928
1107	14-15	0.21549923583207686
1107	16-17	0.1557044203476181
1107	18-19	0.13865079076082054
1107	20-21	0.1379263201259775
1107	22-23	0.1750196982179517
1107	24-25	0.11483824352888661
1107	26-27	0.12386542662008537
1107	28-29	0.1547391919329968
1107	30-31	0.11041787226337618
1107	32-33	0.060063181856676806
1107	34-35	0.11299178107687169
1107	36-37	0.14999237533582033
1107	38-39	0.1940675184225924
1107	40-41	0.1740107646511433
1107	42-43	0.2028210437022011
1107	44-45	0.27978692242092507
1107	46-47	0.23320411304720778
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2216	86-87	0.15503267713502566
2216	88-89	0.11399994263601343
2216	90-91	0.14425033785299846
2216	92-93	0.12834822638155075
2216	94-95	0.06306056041026764
2216	96-97	0.06726693228839409
2216	98-99	0.04852371551633183
2216	100	-0.017567321085110166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1017.0
3	261.0
4	302.0
5	827.0
6	2314.0
7	4048.0
8	4874.0
9	6721.0
10	4747.0
11	3827.0
12	3607.0
13	5049.0
14	5217.0
15	5811.0
16	7167.0
17	8311.0
18	11756.0
19	21433.0
20	43148.0
21	50202.0
22	22562.0
23	13736.0
24	13530.0
25	14329.0
26	15498.0
27	17159.0
28	20194.0
29	24693.0
30	29067.0
31	36397.0
32	47890.0
33	67862.0
34	102488.0
35	202548.0
36	300715.0
37	501534.0
38	528849.0
39	142411.0
40	517.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.13041911538285	40.373274979089004	25.04759845640794	20.44870744912021
2	15.673682823864416	39.539275155091474	25.279016347963246	19.508025673080866
3	18.937009385256125	25.278164056161483	19.048719930626923	36.736106627955465
4	32.191625469223396	26.692759107710053	21.636792522783995	19.47882290028256
5	18.022364654842256	38.234129217036426	21.872930793973165	21.87057533414815
6	36.147486417607425	23.213392976330386	23.51252406964864	17.126596536413548
7	19.670665969921913	22.842788386463027	38.56504022130661	18.921505422308453
8	35.108945319281275	23.124131451467274	23.191041856951312	18.575881372300138
9	34.63145626528274	24.917888632122754	23.17071574941835	17.27993935317615
10-11	17.40636686966603	41.518190121511736	23.088626190669356	17.98681681815287
12-13	17.29313387576997	24.657313167740984	31.28691740185238	26.762635554636667
14-15	17.528127232709505	32.76590343441428	23.017528432560503	26.688440900315708
16-17	26.25991333924797	24.204054055232927	31.40553725042724	18.130495355091863
18-19	17.82026050567517	24.374165255616067	31.59438249198078	26.211191746727973
20-21	26.372339395398626	32.97167255949312	23.03290386797975	17.623084177128508
22-23	25.896289743864877	24.652340686499016	31.591569114435984	17.859800455200126
24-25	17.661381006342967	32.91616832808606	22.906258260207327	26.516192405363647
26-27	18.080120211941157	28.12536325837171	35.838730185039594	17.95578634464755
28-29	25.806915936278962	24.372202434073188	31.982388692752128	17.838492936895722
30-31	23.713784852077406	25.577004106222667	32.22060980067329	18.488601241026632
32-33	20.03079012726935	27.10178930811849	33.06630236698831	19.80111819762385
34-35	19.892105880700576	26.795436483531056	33.37468344050339	19.937774195264975
36-37	19.992994908004736	26.80677723022327	33.644963966958294	19.555263894813702
38-39	20.675555194977967	26.440972722014745	33.20854150146252	19.674930581544768
40-41	19.61683106387545	27.653909199003063	33.12448912117064	19.604770615950848
42-43	19.655090591389175	26.784609801439586	33.58114491743442	19.979154689736813
44-45	19.739398364664325	27.268454666237464	32.90526376395893	20.086883205139276
46-47	20.333958819131663	26.608052453570547	33.33800048677975	19.719988240518045
48-49	19.771021600632988	26.963846571910366	33.17670017421131	20.08843165324533
50-51	20.171306340611476	27.484343226826276	32.440969232554224	19.903381200008024
52-53	20.348326976693873	27.19007456983638	32.39648506335121	20.065113390118537
54-55	20.567142018426097	28.02985495184981	30.586931621404002	20.816071408320095
56-57	20.909719805043842	28.65610406967057	29.587877265205105	20.846298860080484
58-59	21.732403741050625	28.991070470527582	28.475088305160302	20.801437483261495
60-61	21.35702502553849	29.37063653866453	28.250083528961213	21.022254906835766
62-63	20.54916293239624	29.474270991245156	28.0899356695723	21.886630406786306
64-65	21.009998176756877	29.367757733734972	28.755139321073113	20.867104768435038
66-67	21.113918672888374	28.78582476452684	29.19114741313206	20.90910914945272
68-69	21.346992826541538	28.955630637114428	28.899864696168308	20.797511840175726
70-71	20.1375458100739	29.772120780697005	29.128206268990297	20.962127140238803
72-73	20.20958204631794	29.14997990282257	29.307507751534768	21.33293029932472
74-75	20.43929786667004	29.48458810972458	28.61728080578798	21.4588332178174
76-77	21.213735093947548	28.882421730002804	28.84318695425168	21.06065622179796
78-79	20.92935917634558	29.276432296437427	28.309805584198283	21.484402943018704
80-81	21.130022435221488	29.813894745290337	28.04374333862354	21.012339480864632
82-83	21.002071883451105	29.8802669458257	28.00172293465934	21.115938236063858
84-85	20.889625075514864	30.197418209496224	27.48147841201782	21.431478302971087
86-87	20.81611137669806	30.2983796675937	27.706536986569052	21.178971969139184
88-89	21.778290146897564	29.569470360958523	27.46329741806092	21.18894207408299
90-91	21.168450726833136	30.27982868486101	27.34074204282704	21.210978545478813
92-93	20.794523989604897	30.32509122758349	27.311026084589756	21.56935869822186
94-95	21.024959238739292	30.64780089836161	27.035445939969065	21.29179392293003
96-97	20.96978463018815	29.940985997723534	27.436789327710585	21.652440044377727
98-99	21.6687690776348	30.04018134821247	26.70655140918239	21.584498164970345
100	20.938682327365484	30.83335296154876	26.54293039660336	21.685034314482397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55373.0
1	30003.5
2	3435.5
3	1897.0
4	1475.0
5	1383.5
6	1504.5
7	1644.5
8	1501.5
9	1400.5
10	1386.0
11	2117.0
12	16218.0
13	18199.0
14	6108.0
15	5527.5
16	6273.0
17	7229.0
18	8030.0
19	8962.5
20	10082.0
21	11218.5
22	12655.5
23	14658.5
24	16849.5
25	19095.0
26	22256.0
27	25758.0
28	28330.0
29	30594.0
30	32966.0
31	35673.5
32	38773.5
33	42295.5
34	46365.0
35	49735.5
36	52358.0
37	54786.0
38	56650.0
39	58196.0
40	59500.0
41	61102.5
42	62037.5
43	62918.5
44	65011.0
45	66799.5
46	68666.0
47	71352.5
48	73026.0
49	76515.5
50	91779.5
51	93554.5
52	76265.0
53	68149.5
54	66593.0
55	66181.5
56	61338.0
57	53727.5
58	48385.5
59	40895.5
60	32544.0
61	26660.0
62	21740.0
63	16474.0
64	12138.0
65	9274.0
66	7043.5
67	5342.5
68	4053.0
69	3027.5
70	2437.0
71	1940.0
72	1482.0
73	1095.5
74	760.0
75	551.5
76	389.0
77	263.0
78	163.0
79	79.0
80	35.5
81	18.5
82	13.5
83	9.5
84	3.5
85	0.5
86	0.5
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13752836277129465
2	0.004361825650849815
3	0.0037511700597308404
4	0.0
5	0.0031405144686118664
6	0.013608896030651421
7	0.0022681493384419035
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.1809128254249073E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.978921041359703E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	8.723651301699629E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.542738476274723E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.271369238137361E-4
74-75	2.1809128254249072E-4
76-77	3.925643085764833E-4
78-79	2.617095390509889E-4
80-81	7.633194888987176E-4
82-83	7.851286171529666E-4
84-85	0.0010032198996954573
86-87	8.723651301699629E-5
88-89	0.0
90-91	4.3618256508498146E-5
92-93	0.0
94-95	0.0
96-97	0.0011558837974752008
98-99	5.234190781019778E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2292618.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.88253772089861
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.20540868901911	63.73665413060352
2	6.254354985535729	8.741403963931816
3	1.1320012866217617	2.3732136783735305
4	0.398360807014448	1.1135385649085912
5	0.18662323000738684	0.6520852455293574
6	0.11042155454863337	0.462992307056708
7	0.08145493986430292	0.3984594535334474
8	0.06081335871632396	0.33998334675424385
9	0.04903145874933226	0.3083798489004806
>10	0.4792922857296162	6.1909840937961
>50	0.026066313881624247	1.2658532126413702
>100	0.012676546119765066	1.8498770862188392
>500	0.002121687545179476	1.036792156782894
>1k	0.0011232463474479036	1.8001153917913566
>5k	0.0	0.0
>10k+	2.496102994328675E-4	9.729667519177712
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	146895	6.407303789815836	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50558	2.205251812556649	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	13354	0.5824781974144843	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12307	0.5368098828500867	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4954	0.2160848427430998	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4130	0.18014339938009735	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4119	0.17966359855850383	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3933	0.1715506028479232	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3548	0.15475757409215143	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3363	0.14668819663807925	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	2935	0.12801958285244205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010032198996954573	0.0	0.0	4.3618256508498146E-5	0.0
2	0.0010032198996954573	0.0	0.0	4.3618256508498146E-5	0.0
3	0.0010032198996954573	0.0	0.0	4.3618256508498146E-5	0.0
4	0.0010032198996954573	4.3618256508498146E-5	0.0	4.3618256508498146E-5	0.0
5	0.0010032198996954573	8.723651301699629E-5	0.0	8.723651301699629E-5	0.0
6	0.0010032198996954573	8.723651301699629E-5	0.0	8.723651301699629E-5	0.0
7	0.0010032198996954573	8.723651301699629E-5	0.0	8.723651301699629E-5	0.0
8	0.0010032198996954573	8.723651301699629E-5	0.0	8.723651301699629E-5	0.0
9	0.0010032198996954573	8.723651301699629E-5	0.0	8.723651301699629E-5	0.0
10-11	0.0010032198996954573	1.0904564127124536E-4	0.0	8.723651301699629E-5	0.0
12-13	0.0010250290279497064	1.3085476952549442E-4	0.0	1.7447302603399258E-4	0.0
14-15	0.0010468381562039554	1.7447302603399258E-4	0.0	1.9628215428824165E-4	0.0
16-17	0.0010686472844582046	1.7447302603399258E-4	0.0	2.1809128254249072E-4	0.0
18-19	0.0011122655409667027	2.617095390509889E-4	0.0	2.6170953905098885E-4	0.0
20-21	0.0011340746692209518	3.707551803222342E-4	0.0	3.05327795559487E-4	0.0
22-23	0.0011776929257294498	4.3618256508498143E-4	0.0	3.4894605206798517E-4	0.0
24-25	0.0011776929257294498	4.3618256508498143E-4	0.0	3.4894605206798517E-4	0.0
26-27	0.0011995020539836989	4.3618256508498143E-4	0.0	3.707551803222342E-4	0.0
28-29	0.0013085476952549443	4.798008215934796E-4	0.0	3.925643085764833E-4	0.0
30-31	0.0014394024647804387	5.452282063562268E-4	0.0	3.925643085764833E-4	0.0
32-33	0.0015920663625601825	6.760829758817213E-4	0.0	4.579916933392305E-4	0.0
34-35	0.0020064397993909146	6.978921041359703E-4	0.0	5.234190781019777E-4	0.0
36-37	0.002617095390509889	7.197012323902194E-4	0.0	5.234190781019777E-4	0.0
38-39	0.0032931783663916097	7.415103606444684E-4	0.0	5.234190781019777E-4	0.0
40-41	0.004579916933392305	7.415103606444684E-4	0.0	5.234190781019777E-4	0.0
42-43	0.005997510269918495	7.415103606444684E-4	0.0	5.234190781019777E-4	0.0
44-45	0.008025759197563659	7.415103606444684E-4	0.0	5.234190781019777E-4	0.0
46-47	0.010272099407751313	7.851286171529666E-4	0.0	5.234190781019777E-4	0.0
48-49	0.012583867002701714	8.723651301699629E-4	0.0	5.452282063562268E-4	0.0
50-51	0.015658954086550835	9.15983386678461E-4	0.0	5.670373346104759E-4	0.0
52-53	0.019802688454858157	9.596016431869592E-4	0.0	6.106555911189741E-4	0.0
54-55	0.024818787953335447	0.0011122655409667027	0.0	6.106555911189741E-4	0.0
56-57	0.030489161299440203	0.0011776929257294498	0.0	6.324647193732231E-4	0.0
58-59	0.03729360931476591	0.0011776929257294498	0.0	6.542738476274721E-4	0.0
60-61	0.04678058010536426	0.0012431203104921972	0.0	6.542738476274721E-4	0.0
62-63	0.05836122720837052	0.0013085476952549443	0.0	6.978921041359703E-4	0.0
64-65	0.07166479544346245	0.0013521659517634426	0.0	7.851286171529666E-4	0.0
66-67	0.08786897773636951	0.0013521659517634426	0.0	7.851286171529666E-4	0.0
68-69	0.10935096906680486	0.0013521659517634426	0.0	7.851286171529666E-4	0.0
70-71	0.13811720923415938	0.0013957842082719407	0.0	7.851286171529666E-4	0.0
72-73	0.17290276879968663	0.0014175933365261897	0.0	8.287468736614648E-4	0.0
74-75	0.21508162284340437	0.0014612115930346878	0.0	8.287468736614648E-4	0.0
76-77	0.26286542284846404	0.0014830207212889368	0.0	8.723651301699629E-4	0.0
78-79	0.3213356956981058	0.0015484481060516842	0.0	8.723651301699629E-4	0.0
80-81	0.3943090388368232	0.0016574937473229296	0.0	9.596016431869592E-4	0.0
82-83	0.4823961078557352	0.0017447302603399257	0.0	0.0010032198996954573	0.0
84-85	0.5912454669726923	0.001788348516848424	0.0	0.0010032198996954573	0.0
86-87	0.718087356899405	0.001810157645102673	0.0	0.0010032198996954573	0.0
88	0.8293575292525838	0.001831966773356922	0.0	0.0010032198996954573	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3250	0.0	85.06625	1
GTGGAAT	51975	0.0	70.052345	6
TGGAATC	52320	0.0	69.51096	7
GAATCAC	52495	0.0	69.30308	9
AACGAGT	44220	0.0	69.08135	1
GGAATCA	52720	0.0	68.97164	8
ACGAGTG	48515	0.0	68.80817	2
CGAGTGG	50230	0.0	68.157745	3
AGTGGAA	51950	0.0	67.682236	5
GAGTGGA	52240	0.0	67.28851	4
AACGTGT	1560	0.0	66.11836	1
CGTGTGG	1795	0.0	63.63703	3
ATCGAGT	965	0.0	61.49585	1
ACGTGTG	1830	0.0	58.827568	2
TCGAGTG	1095	0.0	56.67041	2
GTGTGGA	2820	0.0	44.506226	4
TGAGTGG	2380	0.0	42.662357	3
AATGAGT	1955	0.0	42.64117	1
ATGAGTG	2220	0.0	41.50492	2
AGTACTT	24615	0.0	41.40311	20-21
>>END_MODULE