FastQCFastQC Report
Thu 26 May 2016
SRR2132772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051971
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1432626.981677616301594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA873604.257370109031755No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG165040.8042998658363106No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118280.5764214016669826No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71680.34932267561286195No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC52970.2581420497658105No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47710.23250815922837118No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45330.22090955476466284No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39380.19191304360539208No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA39050.19030483374277707No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33120.1614057898479072No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA31620.15409574501783896No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.11501137199307397No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20710.10092735228714245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT32250.083.475221
ATCGAGT9950.067.640091
TCGAGTG10850.063.2566222
AACGTGT14250.060.440421
ACGTGTG14650.060.0049132
CGTGTGG15650.059.7723663
GTGGAAT650050.054.33516
TGGAATC654800.053.9539837
GGAATCA665000.053.1970948
GAATCAC670100.052.820279
AGTGGAA655100.052.4290585
CGAGTGG641050.052.2681923
GAGTGGA657300.052.232124
ACGAGTG624550.052.14632
AACGAGT587400.050.741911
GCGAGTG7900.047.00922
AGCGAGT7500.045.181691
AATGAGT17250.043.108131
ATGAGTG19800.042.0233732
TGAGTGG22600.041.5990333