##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132772_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2051971
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.916644533475374	31.0	25.0	33.0	16.0	34.0
2	28.208790475109055	31.0	25.0	34.0	16.0	34.0
3	29.28602012406608	31.0	28.0	34.0	16.0	34.0
4	34.77225701532819	35.0	35.0	37.0	32.0	37.0
5	33.87360542619754	35.0	35.0	37.0	28.0	37.0
6	35.26302467237598	37.0	35.0	37.0	32.0	37.0
7	35.671224398395495	37.0	35.0	37.0	33.0	37.0
8	35.92655451758334	37.0	35.0	37.0	35.0	37.0
9	37.89009493798889	39.0	38.0	39.0	35.0	39.0
10-11	37.885065139809484	39.0	38.0	39.0	35.0	39.0
12-13	37.8510907317891	39.0	38.0	39.0	35.0	39.0
14-15	39.263099722169564	41.0	39.0	41.0	36.0	41.0
16-17	39.182643906760866	40.5	39.0	41.0	36.0	41.0
18-19	38.912202950236626	40.0	38.5	41.0	35.5	41.0
20-21	38.7709604570435	40.0	39.0	41.0	35.0	41.0
22-23	38.59129271320111	40.0	38.0	41.0	35.0	41.0
24-25	38.380498554804134	40.0	38.0	41.0	35.0	41.0
26-27	38.135207807517745	40.0	38.0	41.0	34.0	41.0
28-29	37.94960138325541	40.0	38.0	41.0	34.0	41.0
30-31	37.79459310097462	40.0	38.0	41.0	33.5	41.0
32-33	37.63404916541218	40.0	37.0	41.0	33.0	41.0
34-35	37.51776584561867	40.0	37.0	41.0	33.0	41.0
36-37	37.310986363842375	40.0	37.0	41.0	33.0	41.0
38-39	36.806958285472845	40.0	35.5	41.0	32.5	41.0
40-41	36.53098630536202	39.5	35.0	41.0	31.5	41.0
42-43	36.33078123423772	39.0	35.0	41.0	31.0	41.0
44-45	36.17515890819119	39.0	35.0	41.0	31.0	41.0
46-47	36.00436750811781	39.0	35.0	41.0	31.0	41.0
48-49	35.82497096693862	39.0	35.0	41.0	30.0	41.0
50-51	35.51504163557867	39.0	35.0	41.0	29.5	41.0
52-53	35.016351839280375	39.0	35.0	41.0	27.0	41.0
54-55	34.153282137028256	38.0	35.0	40.5	18.5	41.0
56-57	33.45263992522312	38.0	34.0	40.0	4.5	41.0
58-59	33.10667304752357	37.5	34.0	40.0	2.0	41.0
60-61	32.84112251099066	37.0	34.0	40.0	2.0	41.0
62-63	32.568799705258996	36.5	34.0	40.0	2.0	41.0
64-65	32.28386609752282	36.0	33.0	40.0	2.0	41.0
66-67	31.962193910147853	35.5	33.0	39.0	2.0	41.0
68-69	31.650783076369013	35.0	33.0	39.0	2.0	41.0
70-71	31.338499179569304	35.0	33.0	39.0	2.0	40.5
72-73	31.036142079980664	35.0	33.0	37.5	2.0	40.0
74-75	30.70611767905102	35.0	32.5	37.0	2.0	39.5
76-77	30.379085035802163	35.0	32.0	36.5	2.0	39.0
78-79	30.05193153314545	35.0	32.0	36.0	2.0	39.0
80-81	29.745365553411816	35.0	32.0	36.0	2.0	38.0
82-83	29.443440477472635	35.0	31.0	35.0	2.0	37.0
84-85	29.171205148610774	35.0	31.0	35.0	2.0	37.0
86-87	28.96559649234809	35.0	31.0	35.0	2.0	36.0
88-89	28.73884840477765	35.0	31.0	35.0	2.0	36.0
90-91	28.53248486455218	35.0	31.0	35.0	2.0	36.0
92-93	28.359602060652904	35.0	30.5	35.0	2.0	35.5
94-95	28.158473243530246	34.5	30.0	35.0	2.0	35.0
96-97	27.94840570358938	34.0	30.0	35.0	2.0	35.0
98-99	27.769621013162467	34.0	30.0	35.0	2.0	35.0
100	27.660768597606886	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.08906525297616952
1101	2	2.2704732963465695
1101	3	1.1422770008540084
1101	4	0.5936835509424583
1101	5	2.1558286493713084
1101	6	0.9774710307500101
1101	7	0.5428862895800037
1101	8	0.4249172457381434
1101	9	0.35553409896066057
1101	10-11	0.46967300177919924
1101	12-13	0.43018936369266925
1101	14-15	0.537746744789132
1101	16-17	0.4802030613937518
1101	18-19	0.46138869850568653
1101	20-21	0.46305513907829976
1101	22-23	0.45463257864484063
1101	24-25	0.3686919076879178
1101	26-27	0.3018180267404844
1101	28-29	0.3528744175566132
1101	30-31	0.40762634273366416
1101	32-33	0.43300008993424655
1101	34-35	0.42096949573561204
1101	36-37	0.45371650328622337
1101	38-39	0.4707151035746051
1101	40-41	0.4805966862536266
1101	42-43	0.490334005932624
1101	44-45	0.5042184850772955
1101	46-47	0.4823480468756287
1101	48-49	0.4641917824402242
1101	50-51	0.39918828096499936
1101	52-53	0.49327177110752274
1101	54-55	0.6209897200100087
1101	56-57	0.5448879288114341
1101	58-59	0.5356578377780039
1101	60-61	0.4847985109860886
1101	62-63	0.4812698794675754
1101	64-65	0.5063196967368171
1101	66-67	0.3999095713534153
1101	68-69	0.42957674170451554
1101	70-71	0.44164627632100206
1101	72-73	0.41918450131325
1101	74-75	0.5295077581456589
1101	76-77	0.4523604243805046
1101	78-79	0.4835283995039319
1101	80-81	0.4780487027870386
1101	82-83	0.5209076236126009
1101	84-85	0.5366337578321918
1101	86-87	0.6406574735617525
1101	88-89	0.62099614954505
1101	90-91	0.5173001419557153
1101	92-93	0.5558146738087473
1101	94-95	0.5730732062361419
1101	96-97	0.569837807121111
1101	98-99	0.6055582884274777
1101	100	0.6749957901580181
1102	1	1.4772864865549522
1102	2	3.1349890215550538
1102	3	3.209856771754083
1102	4	1.4318038872524212
1102	5	2.59582179092493
1102	6	1.213830105191434
1102	7	0.6881545500597284
1102	8	0.4818447580587417
1102	9	0.4030526467247597
1102	10-11	0.49550559149002993
1102	12-13	0.41750324407536965
1102	14-15	0.5558992922579264
1102	16-17	0.48456595880380604
1102	18-19	0.37920958498931157
1102	20-21	0.31176153016595975
1102	22-23	0.23305808371966208
1102	24-25	0.2712942119547108
1102	26-27	0.3752413865446371
1102	28-29	0.4754631279760986
1102	30-31	0.5186771348253743
1102	32-33	0.4851793853438906
1102	34-35	0.5776298924341461
1102	36-37	0.5254251177516593
1102	38-39	0.6069673424668025
1102	40-41	0.6761044038782273
1102	42-43	0.7622514766745567
1102	44-45	0.7556626641715525
1102	46-47	0.7529148263161289
1102	48-49	0.6546119719516099
1102	50-51	0.7427751788715184
1102	52-53	0.8522092575992772
1102	54-55	0.8814693137246294
1102	56-57	0.7893158423671878
1102	58-59	0.9103958312170022
1102	60-61	0.8446473362765232
1102	62-63	0.8300300360669866
1102	64-65	0.8073106673407224
1102	66-67	0.80462344253446
1102	68-69	0.7633684193382777
1102	70-71	0.7659504810515116
1102	72-73	0.8040964810182416
1102	74-75	0.7905514884985081
1102	76-77	0.7906762959595746
1102	78-79	0.7740594369161684
1102	80-81	0.7728591806955656
1102	82-83	0.8178574874066378
1102	84-85	0.7992439102587561
1102	86-87	0.7790844070337535
1102	88-89	0.8591175825644584
1102	90-91	0.8812561441127578
1102	92-93	0.862825241807947
1102	94-95	0.9388152398457379
1102	96-97	0.8975253604853854
1102	98-99	0.8665190968467833
1102	100	0.858488387101783
1103	1	1.4159973528392662
1103	2	2.9394014798416954
1103	3	3.159411110747982
1103	4	1.3823171570075417
1103	5	2.5941041655727446
1103	6	1.2333652216850837
1103	7	0.7249067933803204
1103	8	0.4778698936785162
1103	9	0.4039332728384011
1103	10-11	0.4502466668468941
1103	12-13	0.4481463911236645
1103	14-15	0.44188905913122056
1103	16-17	0.3354686177800943
1103	18-19	0.28807325806069173
1103	20-21	0.2137705778807657
1103	22-23	0.12494410450722171
1103	24-25	0.1503559105959198
1103	26-27	0.2796797283895174
1103	28-29	0.38537651357047054
1103	30-31	0.35830659207004345
1103	32-33	0.3618518456939981
1103	34-35	0.3764875825141587
1103	36-37	0.3169361109067168
1103	38-39	0.3229824585336587
1103	40-41	0.42225635430501285
1103	42-43	0.3576856531424255
1103	44-45	0.3903912744611091
1103	46-47	0.41692795499380964
1103	48-49	0.4596179106277134
1103	50-51	0.44215580631891527
1103	52-53	0.4287416817037908
1103	54-55	0.46171699218543694
1103	56-57	0.5475271387677481
1103	58-59	0.508380243773388
1103	60-61	0.5156278576082158
1103	62-63	0.4559248495199313
1103	64-65	0.4384201400776675
1103	66-67	0.3929237214108703
1103	68-69	0.3492007250306699
1103	70-71	0.3430424822145497
1103	72-73	0.4075853230792319
1103	74-75	0.35269698249198456
1103	76-77	0.46310338809411533
1103	78-79	0.4722094336850233
1103	80-81	0.4842846118250357
1103	82-83	0.5013916635561237
1103	84-85	0.4768616109641073
1103	86-87	0.550792843206473
1103	88-89	0.49534650129688274
1103	90-91	0.5799426137666508
1103	92-93	0.5634862362924586
1103	94-95	0.5825371819352334
1103	96-97	0.5883454398966279
1103	98-99	0.5415385891108464
1103	100	0.659137231458562
1104	1	6.090850155809495
1104	2	5.103149913710073
1104	3	4.129696710930112
1104	4	1.8399783082679946
1104	5	2.819745808319084
1104	6	1.3146992867989553
1104	7	0.7088844705620545
1104	8	0.5585459818549978
1104	9	0.5186551816925444
1104	10-11	0.5329466473067725
1104	12-13	0.41654374400524574
1104	14-15	0.5437423910181209
1104	16-17	0.46203155993294587
1104	18-19	0.3832215876602234
1104	20-21	0.3163571581472837
1104	22-23	0.2646670792609598
1104	24-25	0.30888555190547606
1104	26-27	0.3208511907263869
1104	28-29	0.3335597185815615
1104	30-31	0.3537008358171718
1104	32-33	0.4673669748011875
1104	34-35	0.4453904598833631
1104	36-37	0.34478975457953
1104	38-39	0.355225715422371
1104	40-41	0.35044871535244937
1104	42-43	0.3671058597343446
1104	44-45	0.3563596520598722
1104	46-47	0.3151082462716488
1104	48-49	0.3919534521977184
1104	50-51	0.3504295082630975
1104	52-53	0.21388023465825512
1104	54-55	0.2066869749713689
1104	56-57	0.1173786256194802
1104	58-59	0.12898118561708571
1104	60-61	0.16978414169951606
1104	62-63	0.18107724846895223
1104	64-65	0.11918350032653535
1104	66-67	0.1464593391659399
1104	68-69	0.08377271325523594
1104	70-71	0.13151207428037281
1104	72-73	0.11842590055152868
1104	74-75	0.13238384999992547
1104	76-77	0.1889403908931122
1104	78-79	0.1972197675609948
1104	80-81	0.14420671308434407
1104	82-83	0.1516947411992149
1104	84-85	0.17748679605613304
1104	86-87	0.14741380157449058
1104	88-89	0.1100676282169637
1104	90-91	0.1898060370476422
1104	92-93	0.1758467454286574
1104	94-95	0.20894270841473173
1104	96-97	0.2192989418171365
1104	98-99	0.24557345941925846
1104	100	0.1728009942141675
1105	1	1.5749443531607028
1105	2	2.9357436891689197
1105	3	3.138825155205481
1105	4	1.3355855834739003
1105	5	2.551242162454514
1105	6	1.1557257357878186
1105	7	0.6202341267670732
1105	8	0.4717162664702599
1105	9	0.3417945749597564
1105	10-11	0.42462313711062905
1105	12-13	0.36285711294060974
1105	14-15	0.4600579926928816
1105	16-17	0.39170988863454426
1105	18-19	0.38296318970651555
1105	20-21	0.31202097937809725
1105	22-23	0.2419202180705824
1105	24-25	0.31966074253307397
1105	26-27	0.3696603128491489
1105	28-29	0.4109384469613033
1105	30-31	0.4910885986268383
1105	32-33	0.49665688606879144
1105	34-35	0.4794695648216205
1105	36-37	0.5144477962326448
1105	38-39	0.4898670956013049
1105	40-41	0.5105594113705578
1105	42-43	0.5352144432996653
1105	44-45	0.5733203634452408
1105	46-47	0.5547324750994704
1105	48-49	0.45469819867185635
1105	50-51	0.40349813452370853
1105	52-53	0.5118902753568406
1105	54-55	0.565641787785836
1105	56-57	0.5723872291840735
1105	58-59	0.5542191356551314
1105	60-61	0.61440973911089
1105	62-63	0.7166997890806357
1105	64-65	0.7703390278439599
1105	66-67	0.7822347925163164
1105	68-69	0.7694956463019977
1105	70-71	0.8091299288878062
1105	72-73	0.8220399716276567
1105	74-75	0.782745025103118
1105	76-77	0.7306713976936692
1105	78-79	0.7921029464645315
1105	80-81	0.8155275473884025
1105	82-83	0.6702317730656269
1105	84-85	0.6789409868239069
1105	86-87	0.7707810603670957
1105	88-89	0.7373496584480286
1105	90-91	0.7037814740041206
1105	92-93	0.7485403991943151
1105	94-95	0.7844971302653754
1105	96-97	0.8596763685469888
1105	98-99	0.803467656350108
1105	100	0.8246879015176525
1106	1	-0.5272544987861707
1106	2	1.876820329260969
1106	3	2.6541953240002947
1106	4	1.1655072577776693
1106	5	2.398110183224574
1106	6	1.0450514077574766
1106	7	0.5424912161534365
1106	8	0.3947536108890759
1106	9	0.2779436910970219
1106	10-11	0.3783538574216436
1106	12-13	0.3046135873425797
1106	14-15	0.41350908118031526
1106	16-17	0.25707781626253734
1106	18-19	0.1281495129140211
1106	20-21	0.10394315619669925
1106	22-23	0.0014865255611340444
1106	24-25	0.005278414277391619
1106	26-27	-0.014816707788909866
1106	28-29	0.05220770403300179
1106	30-31	0.08136966561963277
1106	32-33	0.002128828510684855
1106	34-35	0.03985770201722261
1106	36-37	0.05704226141416058
1106	38-39	0.13096384451125687
1106	40-41	0.10248590603350038
1106	42-43	0.0813985175271199
1106	44-45	0.15331492707360894
1106	46-47	0.15630166197536255
1106	48-49	0.22254407548005162
1106	50-51	0.31593915996649713
1106	52-53	0.39777836752349316
1106	54-55	0.3868215955718526
1106	56-57	0.29871431864035003
1106	58-59	0.32977585999860537
1106	60-61	0.33723793537459557
1106	62-63	0.36199714257104176
1106	64-65	0.33199985993780246
1106	66-67	0.36547431896805094
1106	68-69	0.2870144566824244
1106	70-71	0.3468113862959754
1106	72-73	0.34417283046416713
1106	74-75	0.3181840408977905
1106	76-77	0.2790389441176906
1106	78-79	0.3289009729218151
1106	80-81	0.35405135454278636
1106	82-83	0.30720925735792903
1106	84-85	0.23316048711361148
1106	86-87	0.225104353410142
1106	88-89	0.2548093760445589
1106	90-91	0.25130383324136574
1106	92-93	0.3043880968991566
1106	94-95	0.29594065142219605
1106	96-97	0.3309307105506889
1106	98-99	0.3247649917466866
1106	100	0.34363834459016473
1107	1	-0.48745310829854915
1107	2	1.8989065197703567
1107	3	-0.5077952088499309
1107	4	0.057692523505672
1107	5	1.911312164388896
1107	6	0.7762249058591166
1107	7	0.33317981187656187
1107	8	0.29532583956290637
1107	9	0.23700795991782542
1107	10-11	0.3219102701064003
1107	12-13	0.27280465271998366
1107	14-15	0.4445086833151848
1107	16-17	0.27649792783479654
1107	18-19	0.18589309053768233
1107	20-21	0.16321902465636384
1107	22-23	0.10534369197927873
1107	24-25	-0.04549091916248926
1107	26-27	-0.022279569459364268
1107	28-29	-0.08974696144573358
1107	30-31	-0.03708375270494457
1107	32-33	-0.0011310301281710622
1107	34-35	-0.0028641290465216684
1107	36-37	-0.00967712804989418
1107	38-39	0.04949509307664357
1107	40-41	-9.528643932199543E-4
1107	42-43	0.10595925061912226
1107	44-45	0.12735669271064154
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2216	92-93	0.028001396207262275
2216	94-95	-0.020616812295113363
2216	96-97	-0.049765586777109405
2216	98-99	-0.0731041541629196
2216	100	-0.09700247823307606
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	915.0
3	273.0
4	387.0
5	1166.0
6	3547.0
7	6481.0
8	7789.0
9	10701.0
10	7622.0
11	5166.0
12	5182.0
13	6999.0
14	7483.0
15	8733.0
16	9819.0
17	10943.0
18	14381.0
19	24004.0
20	45307.0
21	51633.0
22	23628.0
23	14963.0
24	14776.0
25	15936.0
26	16977.0
27	18808.0
28	22458.0
29	25975.0
30	29946.0
31	36333.0
32	46599.0
33	66250.0
34	96852.0
35	209999.0
36	255380.0
37	403794.0
38	413605.0
39	110651.0
40	510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.977876728106102	42.54825617940679	24.861009709799205	19.612857382687903
2	14.318917285611244	42.30106049086691	24.84419166812874	18.535830555393105
3	17.836115236028387	26.291234484449237	18.734797574727338	37.13785270479504
4	32.301577361473434	28.187191729317814	21.086409115918308	18.42482179329045
5	16.80686889381978	40.654227242135036	21.77359025730573	20.765313606739454
6	36.71056171626427	24.39007034125647	23.427947002308315	15.47142094017094
7	18.798165419294094	24.14912328154264	39.4038636141006	17.64884768506267
8	35.82740691754416	24.41974082479723	22.81869480611568	16.93415745154293
9	34.91457725279743	26.203196828805087	23.23258954439415	15.649636374003336
10-11	15.967501490030806	44.53067319177513	22.856999441025238	16.644825877168827
12-13	15.735431933492238	25.99239950272202	31.934564377371803	26.337604186413945
14-15	16.049349625311468	34.81279706194678	22.801686768477722	26.336166544264028
16-17	25.65209254906624	25.314904547871294	32.02674404267897	17.006258860383504
18-19	16.535686907855908	25.580795245156974	32.34809361340877	25.535424233578354
20-21	25.73818051034834	35.121670822833266	23.121135727551707	16.019012939266688
22-23	24.969724230995467	26.046079598590815	32.57204902018596	16.412147150227756
24-25	16.022107524911412	35.21526376347424	22.87334957462849	25.889279136985856
26-27	16.827455163840035	29.773032854752824	36.51494100062818	16.884570980778967
28-29	25.005275415685702	25.889181669721452	32.580650506269336	16.52489240832351
30-31	22.473100253366155	27.41936898718354	32.90214627789574	17.205384481554564
32-33	18.71371476497475	29.139934238836705	33.60788237260663	18.538468623581913
34-35	18.53513036977618	28.97177884092904	33.833933325568445	18.659157463726338
36-37	18.814983252687295	28.864589216904136	34.14334315640913	18.17708437399944
38-39	19.49981261918419	28.109022983268282	33.76485340192429	18.626310995623232
40-41	18.22879075776412	29.845840901260303	33.518163755725595	18.407204585249985
42-43	18.108304888817845	28.636495661683437	34.40776764323462	18.847431806264094
44-45	18.355693136014107	29.458335424818383	33.22359331588994	18.96237812327757
46-47	19.112697011799877	28.383490799821242	33.88751595417284	18.61629623420604
48-49	18.27250482584793	28.921973068820172	33.55807660049776	19.247445504834133
50-51	19.004995684636867	29.74043005481072	32.587643782490105	18.666930478062312
52-53	19.33465824028445	29.030009239910175	32.778849377768076	18.856483142037302
54-55	19.48602100127146	30.116799896294832	30.679356579600785	19.717822522832925
56-57	19.611047134681726	30.862107700352492	29.612041300778618	19.914803864187164
58-59	20.717982856482863	30.91364351640447	28.413267049095726	19.95510657801694
60-61	20.359132755774816	31.385969879691284	28.10539232766935	20.149505036864557
62-63	19.325126914561658	31.476029631997722	27.805388087843347	21.393455365597273
64-65	19.944969982519247	31.36126192816565	28.707130851264466	19.986637238050637
66-67	20.360546031108626	30.764299300526176	28.87175306083761	20.00340160752759
68-69	20.524071733957253	30.93045661951363	28.652329881855053	19.893141764674063
70-71	18.68162366817075	32.278307052097716	28.7852752304979	20.25479404923364
72-73	18.925042922355736	31.343602534356158	28.987124998964404	20.744229544323698
74-75	19.367513424330685	31.646258987424	28.231555662878183	20.75467192536713
76-77	20.353711029590038	30.806144864111463	28.624642566914765	20.215501539383734
78-79	19.958254607904724	31.321641764495755	27.96307730694949	20.75702632065003
80-81	20.11065574469547	31.989773627469877	27.787550327140114	20.112020300694535
82-83	19.93961355305598	32.064784213849215	27.66896667341634	20.326635559678465
84-85	19.963049664866503	32.31599700479082	26.999598185817497	20.721355144525177
86-87	19.833126296619202	32.572950592381666	27.23488782248872	20.359035288510412
88-89	21.179441619788975	31.58392589368953	27.053087007564926	20.183545478956574
90-91	20.25683130280445	32.445487128341824	27.007687005094372	20.289994563759354
92-93	19.549325989499852	32.544100282119	27.059714781544187	20.846858946836967
94-95	19.894457572743473	32.76493673643536	26.768458228698165	20.572147462123002
96-97	19.800239283999893	31.743995360522035	27.39609299424208	21.059672361235993
98-99	20.740545985567937	31.896446274023994	26.40593696072875	20.95707077967932
100	19.928351813938892	32.85909011384664	26.123614807421742	21.08894326479273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95470.0
1	51521.5
2	5477.0
3	2827.5
4	2202.5
5	2081.0
6	2132.5
7	2352.0
8	2271.5
9	1966.0
10	1849.0
11	2802.0
12	17271.0
13	19000.5
14	6282.5
15	5584.5
16	6151.0
17	6796.0
18	7540.5
19	8389.5
20	9366.5
21	10532.5
22	11919.0
23	13756.5
24	15699.5
25	17664.0
26	20631.0
27	23736.0
28	25699.0
29	27627.5
30	29842.5
31	32239.0
32	34960.5
33	38396.0
34	42472.5
35	45795.5
36	48672.5
37	50856.5
38	52164.5
39	52524.5
40	52399.5
41	52756.0
42	52821.0
43	53225.5
44	54758.5
45	56440.5
46	58765.5
47	65215.5
48	73184.0
49	79341.0
50	92406.0
51	88638.0
52	65182.5
53	54301.0
54	52227.0
55	52436.5
56	47746.5
57	39045.0
58	33868.5
59	27302.5
60	20718.5
61	16821.0
62	13240.0
63	9973.5
64	7418.0
65	5586.0
66	4456.0
67	3628.0
68	2883.0
69	2181.5
70	1644.5
71	1305.5
72	1009.0
73	717.0
74	522.0
75	374.0
76	244.5
77	159.0
78	104.5
79	66.5
80	33.5
81	14.5
82	8.5
83	7.5
84	3.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1416686688067229
2	0.004434760530241412
3	0.0038012233116354955
4	0.0
5	0.002631616138824574
6	0.012622010739917866
7	0.0028265506676263944
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0011208735406104666
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.4620089660136523E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.8733632200455073E-4
74-75	3.1676860930295796E-4
76-77	6.579040347061435E-4
78-79	3.8986905760364054E-4
80-81	0.001047773092309784
82-83	9.99039460109329E-4
84-85	0.0013401748855125144
86-87	0.0
88-89	0.0
90-91	7.310044830068262E-5
92-93	0.0
94-95	0.0
96-97	7.797381152072811E-4
98-99	5.848035864054609E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2051971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.16855840975444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.03709526152099	55.07439320548971
2	6.670668087928478	8.16070301137076
3	1.4081909476979464	2.584110307090479
4	0.5320237413667873	1.3017250119668167
5	0.28346436239178774	0.8669553204022932
6	0.15241244290472297	0.5593709649714546
7	0.0985382197675399	0.4219208596010788
8	0.0814342309101261	0.3984971607983583
9	0.05669288297066058	0.3121039731067402
>10	0.5978557455174646	7.683875015401921
>50	0.047884408926858815	2.035774270612318
>100	0.027598775473674515	3.2943419112115637
>500	0.0034293296465836313	1.3984639329751305
>1k	0.0022330518628916668	2.667116147750002
>5k	1.5950370449226194E-4	0.6080808220091046
>10k+	3.190074089845239E-4	12.632568085242331
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	143262	6.981677616301594	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	87360	4.257370109031755	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	16504	0.8042998658363106	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11828	0.5764214016669826	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7168	0.34932267561286195	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	5297	0.2581420497658105	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4771	0.23250815922837118	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4533	0.22090955476466284	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.19191304360539208	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	3905	0.19030483374277707	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3312	0.1614057898479072	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	3162	0.15409574501783896	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2360	0.11501137199307397	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2071	0.10092735228714245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001315808069412287	0.0	0.0	9.746726440091015E-5	0.0
2	0.001364541701612742	0.0	0.0	9.746726440091015E-5	0.0
3	0.001364541701612742	0.0	0.0	9.746726440091015E-5	0.0
4	0.001364541701612742	0.0	0.0	9.746726440091015E-5	0.0
5	0.001364541701612742	0.0	0.0	9.746726440091015E-5	0.0
6	0.001364541701612742	0.0	0.0	9.746726440091015E-5	0.0
7	0.001364541701612742	0.0	0.0	9.746726440091015E-5	0.0
8	0.001364541701612742	0.0	0.0	1.4620089660136523E-4	0.0
9	0.0014132753338131972	0.0	0.0	1.4620089660136523E-4	0.0
10-11	0.0016082098626150174	0.0	0.0	1.949345288018203E-4	0.0
12-13	0.0020711793685193407	0.0	0.0	1.949345288018203E-4	0.0
14-15	0.0023392143456218437	0.0	0.0	2.1930134490204782E-4	0.0
16-17	0.0023879479778222985	0.0	0.0	2.680349771025029E-4	0.0
18-19	0.0026072493227243462	0.0	0.0	3.1676860930295796E-4	0.0
20-21	0.0026803497710250293	0.0	0.0	3.898690576036406E-4	0.0
22-23	0.002899651115927077	0.0	0.0	4.1423587370386815E-4	0.0
24-25	0.0030945856447288974	0.0	0.0	4.386026898040957E-4	0.0
26-27	0.003313886989630945	0.0	0.0	4.6296950590432324E-4	0.0
28-29	0.0035575551506332207	0.0	0.0	5.604367703052333E-4	0.0
30-31	0.0037281228633348133	0.0	0.0	6.82270850806371E-4	0.0
32-33	0.004166725553138909	0.0	0.0	6.82270850806371E-4	0.0
34-35	0.004727162323444142	0.0	0.0	7.310044830068261E-4	0.0
36-37	0.005945503128455519	0.0	0.0	8.284717474077363E-4	0.0
38-39	0.008162883393576225	0.0	0.0	8.772053796081914E-4	0.0
40-41	0.01215904123401354	0.0	0.0	9.259390118086465E-4	0.0
42-43	0.017227338982860867	0.0	0.0	9.746726440091015E-4	0.0
44-45	0.021857034041904103	0.0	0.0	9.99039460109329E-4	0.0
46-47	0.026754764078049838	0.0	0.0	0.0010965067245102393	0.0
48-49	0.03279773447090627	0.0	0.0	0.0011696071728109218	0.0
50-51	0.03988847795607248	0.0	0.0	0.0011696071728109218	0.0
52-53	0.050220007982568954	0.0	0.0	0.0011939739889111492	0.0
54-55	0.06486446445880571	0.0	0.0	0.0012427076211116044	0.0
56-57	0.08165320075186247	0.0	0.0	0.0013401748855125144	0.0
58-59	0.09853940430932016	0.0	0.0	0.001364541701612742	0.0
60-61	0.11966543386821743	0.0	0.0	0.001364541701612742	0.0
62-63	0.14171740243892336	0.0	0.0	0.001413275333813197	0.0
64-65	0.16752186068906433	0.0	0.0	0.0014863757821138798	0.0
66-67	0.1981265817109501	0.0	0.0	0.0015594762304145624	0.0
68-69	0.23747898971281758	0.0	0.0	0.0016082098626150174	0.0
70-71	0.2892828407419013	0.0	0.0	0.0018762448397175203	0.0
72-73	0.3437183079098096	0.0	0.0	0.001998078920218658	0.0
74-75	0.39983508538863366	0.0	0.0	0.002119913000719796	0.0
76-77	0.46406601262883346	0.0	0.0	0.002217380265120706	0.0
78-79	0.5393351075624363	0.0	0.0	0.002314847529521616	0.0
80-81	0.631612240133998	0.0	0.0	0.0023879479778222985	0.0
82-83	0.7449910354483567	0.0	0.0	0.0024366816100227537	2.4366816100227537E-5
84-85	0.8780094845394988	0.0	0.0	0.002485415242223209	4.8733632200455075E-5
86-87	1.0219442672435428	0.0	0.0	0.002485415242223209	4.8733632200455075E-5
88	1.1358347656960064	0.0	0.0	0.002534148874423664	4.8733632200455075E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3225	0.0	83.47522	1
ATCGAGT	995	0.0	67.64009	1
TCGAGTG	1085	0.0	63.256622	2
AACGTGT	1425	0.0	60.44042	1
ACGTGTG	1465	0.0	60.004913	2
CGTGTGG	1565	0.0	59.772366	3
GTGGAAT	65005	0.0	54.3351	6
TGGAATC	65480	0.0	53.953983	7
GGAATCA	66500	0.0	53.197094	8
GAATCAC	67010	0.0	52.82027	9
AGTGGAA	65510	0.0	52.429058	5
CGAGTGG	64105	0.0	52.268192	3
GAGTGGA	65730	0.0	52.23212	4
ACGAGTG	62455	0.0	52.1463	2
AACGAGT	58740	0.0	50.74191	1
GCGAGTG	790	0.0	47.0092	2
AGCGAGT	750	0.0	45.18169	1
AATGAGT	1725	0.0	43.10813	1
ATGAGTG	1980	0.0	42.023373	2
TGAGTGG	2260	0.0	41.599033	3
>>END_MODULE