FastQCFastQC Report
Thu 26 May 2016
SRR2132771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734974
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT476186.47886864025122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137261.8675490561570887No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40110.5457335905759931No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG18040.24545085948618592No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16890.2298040474901153No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14670.19959889737596162No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13380.18204725609341282No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11380.1548354091437248No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11230.1527945206224982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT10600.087.075191
AACGAGT121500.081.586831
ACGAGTG136750.079.4759452
CGAGTGG143000.078.265263
GTGGAAT152650.078.264596
GAATCAC153250.077.988839
TGGAATC153000.077.962687
GGAATCA154500.077.2361768
GAGTGGA150700.076.881134
AGTGGAA150650.076.813055
AACGTGT4800.074.561831
ACGTGTG5500.069.2305152
ATCGAGT3100.063.8016361
CGTGTGG6100.063.1872833
ATGAGTG7650.057.147432
TCGAGTG3950.055.9340062
TGAGTGG8300.053.234773
AGCGAGT2150.050.3771481
AATGAGT8400.047.0916861
GTGTGGA9650.042.3748364
Produced by FastQC (version 0.11.5)