FastQCFastQC Report
Thu 26 May 2016
SRR2132770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885317
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1667768.84604551913551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA319481.6945691361187534No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136910.7261908740015605No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG80920.42921163920974564No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57630.30567803716828523No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47510.2520000615281144No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38830.20596005870630774No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32640.17312738388292262No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28680.15212295863242098No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC25260.13398277318880591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT33450.087.406091
AACGAGT418700.080.5336761
ACGAGTG461850.079.688272
CGAGTGG480050.079.071883
AACGTGT14350.079.069931
GTGGAAT509650.079.040886
TGGAATC511050.078.76087
GAATCAC514200.078.139149
GGAATCA515300.077.981458
GAGTGGA501950.077.977814
AGTGGAA502050.077.831215
ATCGAGT10600.077.2840041
TCGAGTG12400.068.2401352
CGTGTGG18100.068.043823
ACGTGTG18400.066.682482
TGAGTGG22200.058.4415973
ATGAGTG21750.055.979572
GCGAGTG6600.054.844852
GTGTGGA25050.051.6021654
AATGAGT22900.049.342591
Produced by FastQC (version 0.11.5)