##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132770_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1885317
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.952593648707353	31.0	25.0	33.0	16.0	34.0
2	28.21112364658039	31.0	25.0	34.0	16.0	34.0
3	29.24697703357048	31.0	28.0	34.0	16.0	34.0
4	34.74668185774594	35.0	35.0	37.0	32.0	37.0
5	33.806403379378644	35.0	35.0	37.0	28.0	37.0
6	35.2483868760532	37.0	35.0	37.0	32.0	37.0
7	35.61592984097634	37.0	35.0	37.0	33.0	37.0
8	35.90937439168055	37.0	35.0	37.0	35.0	37.0
9	37.877342643173534	39.0	38.0	39.0	35.0	39.0
10-11	37.874718946468946	39.0	38.0	39.0	35.0	39.0
12-13	37.871499328760095	39.0	38.0	39.0	35.0	39.0
14-15	39.30520596801493	41.0	39.0	41.0	36.0	41.0
16-17	39.28823906006258	41.0	39.0	41.0	36.0	41.0
18-19	39.217948228335075	41.0	39.0	41.0	36.0	41.0
20-21	39.152006267381026	40.5	39.0	41.0	35.5	41.0
22-23	39.02463484920573	40.0	39.0	41.0	35.0	41.0
24-25	38.94851979799684	40.0	39.0	41.0	35.0	41.0
26-27	38.766929381106735	40.0	39.0	41.0	35.0	41.0
28-29	38.569085198934715	40.0	38.5	41.0	35.0	41.0
30-31	38.416582728527885	40.0	38.0	41.0	34.5	41.0
32-33	38.22822846237529	40.0	38.0	41.0	34.0	41.0
34-35	38.13161128871165	40.0	38.0	41.0	34.0	41.0
36-37	37.950359806865364	40.0	38.0	41.0	33.5	41.0
38-39	37.375972051384466	40.0	36.0	41.0	33.0	41.0
40-41	37.089525528067696	40.0	35.0	41.0	33.0	41.0
42-43	36.91913349320035	40.0	35.0	41.0	33.0	41.0
44-45	36.75788793078299	39.5	35.0	41.0	33.0	41.0
46-47	36.61213843613567	39.0	35.0	41.0	32.0	41.0
48-49	36.426641779605234	39.0	35.0	41.0	31.5	41.0
50-51	36.11341222722757	39.0	35.0	41.0	31.0	41.0
52-53	35.57789963173302	39.0	35.0	41.0	29.0	41.0
54-55	34.58472288745075	39.0	35.0	41.0	22.0	41.0
56-57	33.73573913564668	38.5	34.0	40.5	10.0	41.0
58-59	33.346657617790534	38.0	34.0	40.0	4.0	41.0
60-61	33.084564028224435	37.5	34.0	40.0	2.0	41.0
62-63	32.79829333740692	37.0	34.0	40.0	2.0	41.0
64-65	32.51697168168536	36.5	33.5	40.0	2.0	41.0
66-67	32.19748774344049	36.0	33.0	39.5	2.0	41.0
68-69	31.87457440844166	35.0	33.0	39.0	2.0	41.0
70-71	31.54078412277617	35.0	33.0	39.0	2.0	40.5
72-73	31.206297667713173	35.0	33.0	37.5	2.0	40.0
74-75	30.85613294740354	35.0	33.0	37.0	2.0	39.5
76-77	30.507107558039312	35.0	32.0	36.5	2.0	39.0
78-79	30.16524144215535	35.0	32.0	36.0	2.0	39.0
80-81	29.855777834708963	35.0	32.0	36.0	2.0	37.5
82-83	29.578589436153177	35.0	32.0	35.0	2.0	37.0
84-85	29.317966156354608	35.0	31.0	35.0	2.0	37.0
86-87	29.1033889791478	35.0	31.0	35.0	2.0	36.0
88-89	28.894014640508733	35.0	31.0	35.0	2.0	36.0
90-91	28.69082255132691	35.0	31.0	35.0	2.0	36.0
92-93	28.519080876054268	35.0	31.0	35.0	2.0	35.5
94-95	28.33378816400637	35.0	31.0	35.0	2.0	35.0
96-97	28.14240045573238	34.0	30.5	35.0	2.0	35.0
98-99	27.962383514284333	34.0	30.0	35.0	2.0	35.0
100	27.855863496695783	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2707642987874621
1101	2	2.3917981494436695
1101	3	1.1183292660509352
1101	4	0.6677218486687266
1101	5	2.300622559082612
1101	6	1.0005352367153293
1101	7	0.5659406647455612
1101	8	0.46433463913386674
1101	9	0.37396767736636605
1101	10-11	0.5287988784276081
1101	12-13	0.4297602718393563
1101	14-15	0.567004316086809
1101	16-17	0.5490799612772364
1101	18-19	0.45474286380654405
1101	20-21	0.42988902490142067
1101	22-23	0.398304736658325
1101	24-25	0.432683391781886
1101	26-27	0.35957310944329635
1101	28-29	0.4485148160587613
1101	30-31	0.4008814348096621
1101	32-33	0.41521118799567347
1101	34-35	0.4735048134407478
1101	36-37	0.40356774200894563
1101	38-39	0.4058529999127458
1101	40-41	0.4901166653903104
1101	42-43	0.4812453560520922
1101	44-45	0.5743485199469234
1101	46-47	0.5710244258324693
1101	48-49	0.4391300331539867
1101	50-51	0.5123046276207006
1101	52-53	0.4891476088839113
1101	54-55	0.43904239925743127
1101	56-57	0.3215430203224017
1101	58-59	0.41180858500589324
1101	60-61	0.4106050935200116
1101	62-63	0.373375145563152
1101	64-65	0.40219242963025437
1101	66-67	0.40772817880736056
1101	68-69	0.49719189973979994
1101	70-71	0.4551461636614711
1101	72-73	0.47607810918573534
1101	74-75	0.37971544261124635
1101	76-77	0.3716061712537986
1101	78-79	0.46336106693660994
1101	80-81	0.42474283536002844
1101	82-83	0.5264178664675789
1101	84-85	0.4984493581890952
1101	86-87	0.48022054819010407
1101	88-89	0.4317198640408826
1101	90-91	0.5127005227098742
1101	92-93	0.5570604674371786
1101	94-95	0.6015521795921082
1101	96-97	0.5540126688144547
1101	98-99	0.5574107783914535
1101	100	0.5554653331491082
1102	1	1.566343252237207
1102	2	3.022617908664504
1102	3	3.1831850664558026
1102	4	1.3541476058560065
1102	5	2.640972654391078
1102	6	1.1639131559273324
1102	7	0.6737509200514893
1102	8	0.5079974596888732
1102	9	0.3718322638727116
1102	10-11	0.46197256614574655
1102	12-13	0.4332194049618252
1102	14-15	0.5519230125854051
1102	16-17	0.4539061000241631
1102	18-19	0.395016551622426
1102	20-21	0.32990022105332883
1102	22-23	0.31910716519163884
1102	24-25	0.2633169973304845
1102	26-27	0.35737699130445577
1102	28-29	0.4000366185284534
1102	30-31	0.44141313365684454
1102	32-33	0.4297698234160592
1102	34-35	0.4564033701145078
1102	36-37	0.4780478892551798
1102	38-39	0.5512328605874472
1102	40-41	0.6012755583330502
1102	42-43	0.6271017319022434
1102	44-45	0.6684582580905456
1102	46-47	0.6158267344768191
1102	48-49	0.5357058024876267
1102	50-51	0.6586230212042068
1102	52-53	0.6278161312231703
1102	54-55	0.6223020128262107
1102	56-57	0.49903824458262847
1102	58-59	0.4512456455284308
1102	60-61	0.4006496403044508
1102	62-63	0.40893845697873843
1102	64-65	0.41770615870792227
1102	66-67	0.49921480298946364
1102	68-69	0.3366005300725128
1102	70-71	0.3618423638438877
1102	72-73	0.45519875190349524
1102	74-75	0.4284820143549588
1102	76-77	0.3884374300411757
1102	78-79	0.3605448274992433
1102	80-81	0.33310906485551683
1102	82-83	0.3395275938602822
1102	84-85	0.39238954684696736
1102	86-87	0.42651952497475776
1102	88-89	0.4234842852763876
1102	90-91	0.43127126821829975
1102	92-93	0.4610345692500566
1102	94-95	0.4259251706511513
1102	96-97	0.5035026574890722
1102	98-99	0.4258182566390474
1102	100	0.3993059455558132
1103	1	1.3795932916701261
1103	2	2.975015192372105
1103	3	3.197257915335836
1103	4	1.3786910057363997
1103	5	2.5802381043945815
1103	6	1.2028065204266198
1103	7	0.7157165378542061
1103	8	0.462379826486746
1103	9	0.4247647809704773
1103	10-11	0.42657415723041936
1103	12-13	0.47239112555391216
1103	14-15	0.5378407576881301
1103	16-17	0.49024154464660086
1103	18-19	0.5425464694758588
1103	20-21	0.4864538687378257
1103	22-23	0.5074291709927721
1103	24-25	0.5598434998693804
1103	26-27	0.5346851194057294
1103	28-29	0.5508386417110813
1103	30-31	0.5089281817339923
1103	32-33	0.4679295702515489
1103	34-35	0.5466338464786347
1103	36-37	0.44058713353648216
1103	38-39	0.41085223938098636
1103	40-41	0.5251857368633992
1103	42-43	0.49030097884202917
1103	44-45	0.5636486617203147
1103	46-47	0.5678858150148614
1103	48-49	0.5435724371531592
1103	50-51	0.48962660810103387
1103	52-53	0.4009540033836174
1103	54-55	0.48649369794830477
1103	56-57	0.5329114833059023
1103	58-59	0.46603417862178986
1103	60-61	0.4876336970018542
1103	62-63	0.4365037207844509
1103	64-65	0.4775600574193817
1103	66-67	0.49816556693377834
1103	68-69	0.521366647903239
1103	70-71	0.4463642146421307
1103	72-73	0.4361051053890641
1103	74-75	0.4548464605193594
1103	76-77	0.4372837045037947
1103	78-79	0.38942951779010926
1103	80-81	0.34348028417923615
1103	82-83	0.43455482893153885
1103	84-85	0.5172493610130111
1103	86-87	0.5128250047136795
1103	88-89	0.5131548452072927
1103	90-91	0.5015136995269813
1103	92-93	0.5578436760939347
1103	94-95	0.5295833051299077
1103	96-97	0.5734333981932949
1103	98-99	0.6143555672085803
1103	100	0.49321179731822085
1104	1	6.015995080097266
1104	2	5.0956301879030015
1104	3	4.112814812550436
1104	4	1.8235978527535082
1104	5	2.863402916392424
1104	6	1.3253458352430911
1104	7	0.762936894536665
1104	8	0.5827601081630363
1104	9	0.466443943749006
1104	10-11	0.5023862897832743
1104	12-13	0.3899793251599988
1104	14-15	0.5459522010455942
1104	16-17	0.443571344653094
1104	18-19	0.4058265341377023
1104	20-21	0.36139209287727425
1104	22-23	0.36463897685973023
1104	24-25	0.365720835085412
1104	26-27	0.37309033365218625
1104	28-29	0.3462513477248521
1104	30-31	0.395559030037262
1104	32-33	0.4279643495939851
1104	34-35	0.44942769848992725
1104	36-37	0.434275031957597
1104	38-39	0.457694172825299
1104	40-41	0.5230271953735155
1104	42-43	0.5386258279299696
1104	44-45	0.5752623268454968
1104	46-47	0.4272583905643117
1104	48-49	0.5730813866636311
1104	50-51	0.6594702043066718
1104	52-53	0.6737934215449783
1104	54-55	0.86229496502947
1104	56-57	0.8442408762259461
1104	58-59	0.8096587033188669
1104	60-61	0.7846778953807885
1104	62-63	0.8242356199410708
1104	64-65	0.7500601877067155
1104	66-67	0.7774592227765993
1104	68-69	0.711547309723553
1104	70-71	0.7510227979222854
1104	72-73	0.7712093795018085
1104	74-75	0.7971568039567636
1104	76-77	0.717780821958069
1104	78-79	0.7085721681588915
1104	80-81	0.7306636490929073
1104	82-83	0.8304781629499054
1104	84-85	0.8317534382689722
1104	86-87	0.8445115717457732
1104	88-89	0.7912733584961096
1104	90-91	0.8383924379129724
1104	92-93	0.8139877807643181
1104	94-95	0.8435929788412686
1104	96-97	0.7801262337667509
1104	98-99	0.8471990857334717
1104	100	0.8882057156039735
1105	1	1.4260699386106808
1105	2	2.94776615536718
1105	3	3.1121609578876424
1105	4	1.3479094731221153
1105	5	2.5436019088578448
1105	6	1.1839306867491786
1105	7	0.7245672527289528
1105	8	0.45876640013755576
1105	9	0.3670466322621593
1105	10-11	0.38314825608378555
1105	12-13	0.3926365051352292
1105	14-15	0.4731280537416538
1105	16-17	0.358663701989677
1105	18-19	0.4440018907657688
1105	20-21	0.43139089412748177
1105	22-23	0.4201143883573977
1105	24-25	0.4796798865722067
1105	26-27	0.5228141339034309
1105	28-29	0.5206781162949952
1105	30-31	0.5125431217503902
1105	32-33	0.5226140271497783
1105	34-35	0.47005399165344386
1105	36-37	0.45870482176704996
1105	38-39	0.5684858447304038
1105	40-41	0.6172100427258869
1105	42-43	0.5858067192735348
1105	44-45	0.6446819272817237
1105	46-47	0.735542823677477
1105	48-49	0.6006428372093495
1105	50-51	0.6001251212761929
1105	52-53	0.6609123190070392
1105	54-55	0.7781605159816962
1105	56-57	0.7842630872078544
1105	58-59	0.8020036138463311
1105	60-61	0.7435184314460983
1105	62-63	0.7267901752390102
1105	64-65	0.6881359896264669
1105	66-67	0.6231424078255543
1105	68-69	0.7574779440181238
1105	70-71	0.7365066688900264
1105	72-73	0.7467765197233724
1105	74-75	0.6987658575389304
1105	76-77	0.6832805593051674
1105	78-79	0.744202130666288
1105	80-81	0.7760636660508311
1105	82-83	0.7173160364262472
1105	84-85	0.7276257918826197
1105	86-87	0.864645159253044
1105	88-89	0.7490967115064819
1105	90-91	0.736972504165827
1105	92-93	0.7220764668078488
1105	94-95	0.7108131657877585
1105	96-97	0.7551695300752854
1105	98-99	0.7356086858675717
1105	100	0.6599985482497317
1106	1	-0.5248015232929504
1106	2	2.019958289251207
1106	3	2.751046235645873
1106	4	1.202612417538667
1106	5	2.4747283709185055
1106	6	1.1634231751336017
1106	7	0.6384888968919782
1106	8	0.4632728924203988
1106	9	0.36792463395654096
1106	10-11	0.43976207169985315
1106	12-13	0.3589759636087422
1106	14-15	0.4225736822394026
1106	16-17	0.3962490124667397
1106	18-19	0.34383157791727825
1106	20-21	0.4096688499439338
1106	22-23	0.3339052367054194
1106	24-25	0.30965367572696323
1106	26-27	0.2748111554163586
1106	28-29	0.2778786445669681
1106	30-31	0.24589349034413033
1106	32-33	0.28616819073041455
1106	34-35	0.30539985117078317
1106	36-37	0.30293595109151283
1106	38-39	0.4194609096413089
1106	40-41	0.3866057899138795
1106	42-43	0.4284966246592603
1106	44-45	0.4736660015991916
1106	46-47	0.4449657080154097
1106	48-49	0.48632987900160174
1106	50-51	0.5874426978881502
1106	52-53	0.6268388165296699
1106	54-55	0.7357980772003003
1106	56-57	0.5825337001711048
1106	58-59	0.5934483947553844
1106	60-61	0.5467290711489525
1106	62-63	0.5890640704218697
1106	64-65	0.6753508974869717
1106	66-67	0.6184233226488658
1106	68-69	0.5666439676384947
1106	70-71	0.5940774805118529
1106	72-73	0.6051863725840434
1106	74-75	0.5892532846421297
1106	76-77	0.5802577845335755
1106	78-79	0.5436448371298681
1106	80-81	0.48597574263913756
1106	82-83	0.5010041086827854
1106	84-85	0.44613610308521956
1106	86-87	0.4002930815674901
1106	88-89	0.3777089520069872
1106	90-91	0.4663942276412101
1106	92-93	0.4511115673953974
1106	94-95	0.3425080529435718
1106	96-97	0.4364932791365561
1106	98-99	0.38165503449765836
1106	100	0.38342264751779354
1107	1	-0.9612797157450075
1107	2	1.830063798424657
1107	3	-0.6589713114850291
1107	4	-0.16623255481523813
1107	5	1.8538311910136969
1107	6	0.7193519279372111
1107	7	0.3522251350719543
1107	8	0.3320361592738621
1107	9	0.2706856212882798
1107	10-11	0.3281437141821044
1107	12-13	0.2627682513504084
1107	14-15	0.4062536773988086
1107	16-17	0.3329528726297326
1107	18-19	0.17674794564883456
1107	20-21	0.17191006269229803
1107	22-23	0.2840353480842168
1107	24-25	0.15856868019092474
1107	26-27	0.17664045307422782
1107	28-29	0.19663579894343997
1107	30-31	0.2884394172734801
1107	32-33	0.2448149171388323
1107	34-35	0.2571264098215451
1107	36-37	0.26644724188612656
1107	38-39	0.2519389078112724
1107	40-41	0.19586975301344722
1107	42-43	0.24573726732138823
1107	44-45	0.2380782756176245
1107	46-47	0.24537532629637582
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2216	90-91	-0.02251516186833058
2216	92-93	-0.02260868323783427
2216	94-95	-0.17888393168922434
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2216	98-99	-0.26993457086163986
2216	100	-0.29310778388885694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1077.0
3	242.0
4	242.0
5	495.0
6	1325.0
7	2101.0
8	2675.0
9	3716.0
10	3286.0
11	4608.0
12	3991.0
13	5819.0
14	5796.0
15	6364.0
16	7753.0
17	8858.0
18	12759.0
19	22815.0
20	45222.0
21	53269.0
22	24318.0
23	15362.0
24	15483.0
25	16248.0
26	16627.0
27	18187.0
28	20740.0
29	24109.0
30	27617.0
31	33200.0
32	41932.0
33	58103.0
34	85555.0
35	158579.0
36	245003.0
37	391184.0
38	401798.0
39	98556.0
40	303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.838204973226077	44.22398106651682	23.26038531932726	18.67742864092985
2	14.725717850300125	44.44539924422293	23.072153158371577	17.756729747105364
3	17.710976109787087	23.330180787789836	18.17160491797105	40.78723818445203
4	37.759220332707976	24.56769869470227	19.693717289983596	17.979363682606163
5	16.974208496174555	43.43118658739959	20.331336804450498	19.263268111975353
6	41.03814973215508	22.04879880915373	21.277199601495138	15.635851857196059
7	18.072694328221406	21.411749229816092	43.225617207987845	17.289939233974657
8	39.979854846691566	22.068225131370482	20.899827456072373	17.052092565865582
9	39.455752003509225	23.084287682124543	21.22210747582502	16.23785283854121
10-11	16.223372515073063	45.81600866061251	21.301192319381833	16.659426504932593
12-13	16.05114683631453	22.870610088383014	32.48925777468723	28.588985300615228
14-15	16.396871189301322	33.95458164329924	21.253322385572293	28.39522478182714
16-17	28.030325934577583	22.421110083874492	32.69938158940911	16.84918239213883
18-19	16.59033997996093	22.61219200802836	32.8738084895007	27.923659522510007
20-21	28.170381956986546	34.24336596975469	21.2963390241535	16.28991304910527
22-23	27.758727046963454	22.94889930977125	32.847632520154434	16.444741123110862
24-25	16.38005704080534	34.17085826945813	21.292016143704213	28.15706854603231
26-27	16.82728518720469	27.57680940489552	39.070293786293654	16.52561162160614
28-29	27.132041985512252	22.62598279228374	33.81033534413576	16.43163987806825
30-31	24.674073378641364	23.840659157054226	34.280176755420975	17.20509070888344
32-33	19.616170649286037	25.898933707169668	35.52964832969734	18.955247313846954
34-35	19.069366053560223	25.487040110496007	36.238812889291296	19.204780946652473
36-37	19.224485855694297	25.533530965880008	36.367624118384335	18.874359060041364
38-39	19.950756291912715	25.444235637826424	35.775575142005295	18.829432928255567
40-41	19.05106674368289	26.526732639656885	35.54386344577596	18.87833717088426
42-43	19.10546886292132	25.85378299109346	36.07403700365008	18.966711142335136
44-45	19.151447740618686	26.16570582029441	35.45398466146542	19.228861777621482
46-47	19.53029649655734	25.657727586395286	35.710784976743966	19.101190940303407
48-49	19.059473817930883	25.98255359708739	35.62034925691542	19.337623328066314
50-51	19.540215252925634	26.566513748085868	34.82154459966149	19.07172639932701
52-53	19.83408038092399	26.397349402127663	34.418097178157126	19.350473038791222
54-55	20.266485689144055	27.32132049941734	32.40431184782187	20.00788196361673
56-57	20.57927128435165	28.41219805475684	30.721358795364388	20.287171865527124
58-59	21.384944812994313	29.05325735672038	29.309845506087306	20.251952324198
60-61	20.939422919328685	29.387286063829055	29.082005837745058	20.591285179097202
62-63	20.179466030875474	29.58319962446594	28.82812686474144	21.40920747991715
64-65	20.42895173596801	29.251526401130416	29.81169744928837	20.5078244136132
66-67	20.52016199932478	28.759282592604478	30.27165465321075	20.448900754859995
68-69	20.67283645137661	28.797730036911563	30.22600443320672	20.3034290785051
70-71	19.658046896092273	29.525830404117713	30.45532926293032	20.36079343685969
72-73	19.62257625809682	28.94121278857354	30.679867692705916	20.756343260623726
74-75	19.79207635889636	29.467368055343755	29.924614419663637	20.81594116609625
76-77	20.311636413399203	29.06430043717041	29.92134963767155	20.702713511758834
78-79	20.288849584934862	29.48199669390627	29.20928241641346	21.019871304745408
80-81	20.560994284450366	30.046562992379712	28.70542045000373	20.687022273166196
82-83	20.47343809506634	30.089073640503816	28.64746824633187	20.790020018097973
84-85	20.406351169753414	30.286207881490945	28.086997733240583	21.22044321551506
86-87	20.413729380429633	30.356341077310972	28.213276882981592	21.016652659277803
88-89	21.128303622149485	29.986044787163113	27.871864519335475	21.013787071351928
90-91	20.67671396288103	30.42544845918444	27.95604875892191	20.941788819012615
92-93	20.138098791874256	30.58005629822465	27.84783672984437	21.434008180056725
94-95	20.28118893596617	30.84345011658523	27.463565789501597	21.411795157946997
96-97	20.370596291491054	30.349533174277006	27.73226449461779	21.54760603961415
98-99	20.89262091696715	30.423269057159015	26.85686431139414	21.827245714479695
100	20.31180963201414	31.18499435373468	26.755500533862474	21.74769548038871
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34023.0
1	18788.5
2	2691.0
3	1602.5
4	1369.0
5	1324.5
6	1301.5
7	1430.0
8	1413.5
9	1325.0
10	1361.5
11	2229.0
12	18026.0
13	20530.5
14	7261.5
15	6738.5
16	7483.5
17	8399.5
18	9335.0
19	10310.0
20	11339.5
21	12376.5
22	13813.5
23	15580.5
24	17236.5
25	18813.5
26	20656.0
27	22779.5
28	24767.0
29	26873.5
30	29093.0
31	30900.0
32	32971.5
33	35345.5
34	37818.5
35	40274.5
36	42864.0
37	45201.5
38	46797.0
39	48053.0
40	48650.5
41	49202.0
42	50105.5
43	51667.5
44	53528.5
45	55281.0
46	57052.5
47	59096.5
48	60103.0
49	61101.5
50	68193.5
51	68873.5
52	59703.0
53	54485.0
54	52683.5
55	51344.5
56	46875.5
57	41209.0
58	36628.0
59	30924.5
60	25167.5
61	20657.5
62	16954.5
63	13338.0
64	10196.5
65	7578.0
66	5597.0
67	4364.0
68	3361.5
69	2396.5
70	1679.0
71	1205.0
72	874.5
73	588.0
74	387.0
75	270.5
76	190.5
77	137.5
78	90.5
79	51.5
80	19.0
81	8.0
82	5.5
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13339931693184753
2	0.005569355180057253
3	0.0036068204975608876
4	0.0
5	0.002386866505738823
6	0.014374240512338245
7	0.00281119832898128
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.304147790530717E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.751843854375684E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	3.4476960638449663E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.0608295581061434E-4
64-65	0.0
66-67	2.6520738952653584E-5
68-69	0.0
70-71	0.0
72-73	4.7737330114776456E-4
74-75	5.038940401004182E-4
76-77	5.834562569583788E-4
78-79	5.569355180057253E-4
80-81	0.0011403917749641043
82-83	0.001219953991822065
84-85	0.00140559916449064
86-87	1.3260369476326793E-4
88-89	0.0
90-91	2.6520738952653584E-5
92-93	0.0
94-95	5.304147790530717E-5
96-97	0.0016442858150645223
98-99	7.956221685796076E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1885317.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.68577829473868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.83641622053817	61.48333529391952
2	6.073812696320738	8.22221479133869
3	1.2755186723287253	2.5900342219812464
4	0.5461681890457404	1.4787127582155566
5	0.27535610123368426	0.9318846010103389
6	0.17174127360614383	0.6974665061616906
7	0.11102204174995389	0.5260229312601622
8	0.08139276193594826	0.4407305951354432
9	0.06598954295581856	0.4019898216850804
>10	0.5265126344542028	6.284151927886566
>50	0.019492703647962736	0.8962752331249478
>100	0.013670493448935726	2.0441224141983256
>500	0.0018068481319388065	0.7344256085391883
>1k	7.070275298890981E-4	1.2371764142688453
>5k	1.5711722886424404E-4	0.7367120479518139
>10k+	2.3567584329636606E-4	11.294744833322595
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	166776	8.84604551913551	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31948	1.6945691361187534	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13691	0.7261908740015605	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	8092	0.42921163920974564	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5763	0.30567803716828523	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4751	0.2520000615281144	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3883	0.20596005870630774	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3264	0.17312738388292262	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2868	0.15212295863242098	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	2526	0.13398277318880591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03808378113601055	0.0	0.0	0.0	0.0
2	0.045085256219511094	0.0	0.0	0.0	0.0
3	0.052935394949496554	0.0	0.0	0.0	0.0
4	0.05431447337503454	0.0	0.0	0.0	0.0
5	0.05442055633084516	2.1216591162122867E-4	0.0	0.0	5.304147790530717E-5
6	0.05442055633084516	2.6520738952653586E-4	0.0	0.0	5.304147790530717E-5
7	0.05447359780875047	3.18248867431843E-4	0.0	0.0	5.304147790530717E-5
8	0.05484488815408761	3.712903453371502E-4	0.0	0.0	5.304147790530717E-5
9	0.05627700805753091	4.2433182324245734E-4	0.0	0.0	5.304147790530717E-5
10-11	0.056436132491246835	4.2433182324245734E-4	0.0	0.0	1.0608295581061434E-4
12-13	0.05648917396915214	4.2433182324245734E-4	0.0	0.0	2.1216591162122867E-4
14-15	0.05648917396915214	4.2433182324245734E-4	0.0	0.0	2.1216591162122867E-4
16-17	0.056542215447057446	4.5085256219511095E-4	0.0	0.0	2.1216591162122867E-4
18-19	0.056542215447057446	5.304147790530717E-4	0.0	0.0	2.6520738952653586E-4
20-21	0.0565687361860101	5.834562569583788E-4	0.0	0.0	2.6520738952653586E-4
22-23	0.05670133988077337	5.834562569583788E-4	0.0	0.0	3.18248867431843E-4
24-25	0.05670133988077337	5.834562569583788E-4	0.0	0.0	3.18248867431843E-4
26-27	0.05670133988077337	5.834562569583788E-4	0.0	0.0	3.18248867431843E-4
28-29	0.05680742283658398	6.36497734863686E-4	0.0	0.0	3.18248867431843E-4
30-31	0.0569665472702999	6.895392127689933E-4	0.0	0.0	3.4476960638449663E-4
32-33	0.05739087909354236	7.160599517216468E-4	0.0	0.0	3.712903453371502E-4
34-35	0.05744392057144766	7.425806906743004E-4	0.0	0.0	3.712903453371502E-4
36-37	0.0577091279609742	7.425806906743004E-4	0.0	0.0	4.2433182324245734E-4
38-39	0.058584312346411774	7.691014296269539E-4	0.0	0.0	4.2433182324245734E-4
40-41	0.05975122486032852	7.956221685796076E-4	0.0	0.0	4.2433182324245734E-4
42-43	0.061581155848061626	7.956221685796076E-4	0.0	0.0	4.2433182324245734E-4
44-45	0.06314587944626818	8.486636464849148E-4	0.0	0.0	4.2433182324245734E-4
46-47	0.06540014225724375	9.547466022955291E-4	0.0	0.0	4.7737330114776456E-4
48-49	0.06784005024088788	9.812673412481828E-4	0.0	0.0	4.7737330114776456E-4
50-51	0.0714203499994961	0.0010608295581061434	0.0	0.0	4.7737330114776456E-4
52-53	0.07598191709935252	0.0011934332528694114	0.0	0.0	4.7737330114776456E-4
54-55	0.08173691745207835	0.0012995162086800256	0.0	0.0	4.7737330114776456E-4
56-57	0.08767756297747276	0.00140559916449064	0.0	0.0	4.7737330114776456E-4
58-59	0.09505032840631045	0.0014321199034432936	0.0	0.0	4.7737330114776456E-4
60-61	0.10528733364203474	0.0014586406423959472	0.0	0.0	4.7737330114776456E-4
62-63	0.11756643577711334	0.0015116821203012545	0.0	0.0	4.7737330114776456E-4
64-65	0.1310124504261087	0.0016442858150645223	0.0	0.0	4.7737330114776456E-4
66-67	0.14870178330752865	0.0016973272929698294	0.0	0.0	4.7737330114776456E-4
68-69	0.17132397363414217	0.0017503687708751367	0.0	0.0	4.7737330114776456E-4
70-71	0.20047026574310844	0.0017503687708751367	0.0	0.0	4.7737330114776456E-4
72-73	0.23354162721706748	0.0017503687708751367	0.0	0.0	4.7737330114776456E-4
74-75	0.2733757771239531	0.0017503687708751367	0.0	0.0	5.038940401004182E-4
76-77	0.3209009413271084	0.0017503687708751367	0.0	0.0	5.304147790530717E-4
78-79	0.37542758061376413	0.0017768895098277903	0.0	0.0	5.569355180057253E-4
80-81	0.4437450041557998	0.0018034102487804438	0.0	0.0	5.834562569583788E-4
82-83	0.5243680505718666	0.0018034102487804438	0.0	0.0	5.834562569583788E-4
84-85	0.6155463510910897	0.001856451726685751	0.0	2.6520738952653584E-5	5.834562569583788E-4
86-87	0.7282064501619621	0.0020155761604016727	0.0	5.304147790530717E-5	5.834562569583788E-4
88	0.8236280689136097	0.002121659116212287	0.0	5.304147790530717E-5	5.834562569583788E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3345	0.0	87.40609	1
AACGAGT	41870	0.0	80.533676	1
ACGAGTG	46185	0.0	79.68827	2
CGAGTGG	48005	0.0	79.07188	3
AACGTGT	1435	0.0	79.06993	1
GTGGAAT	50965	0.0	79.04088	6
TGGAATC	51105	0.0	78.7608	7
GAATCAC	51420	0.0	78.13914	9
GGAATCA	51530	0.0	77.98145	8
GAGTGGA	50195	0.0	77.97781	4
AGTGGAA	50205	0.0	77.83121	5
ATCGAGT	1060	0.0	77.284004	1
TCGAGTG	1240	0.0	68.240135	2
CGTGTGG	1810	0.0	68.04382	3
ACGTGTG	1840	0.0	66.68248	2
TGAGTGG	2220	0.0	58.441597	3
ATGAGTG	2175	0.0	55.97957	2
GCGAGTG	660	0.0	54.84485	2
GTGTGGA	2505	0.0	51.602165	4
AATGAGT	2290	0.0	49.34259	1
>>END_MODULE