FastQCFastQC Report
Thu 26 May 2016
SRR2132769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791473
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1169476.527980047703761No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA543093.0315276869927708No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94180.5257126398220905No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG55960.31236864859252694No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44550.24867804315219935No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40890.2282479278225237No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32810.18314537813296655No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30530.17041842104234894No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28190.15735654402829405No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC22490.12553915130175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT23700.085.224691
AACGAGT288350.080.318091
ACGAGTG321600.078.487942
GTGGAAT358500.077.383926
CGAGTGG338450.077.322123
TGGAATC360000.077.08997
GAATCAC361750.076.794929
GGAATCA362750.076.5961768
GAGTGGA354100.075.878564
AGTGGAA355200.075.4715355
AACGTGT11000.074.475441
ATCGAGT8850.072.884271
ACGTGTG12450.070.6151352
TCGAGTG9700.068.8243562
CGTGTGG13600.067.401993
AGCGAGT5900.051.072191
GCGAGTG6950.045.999132
GTGTGGA21550.045.3700074
TGAGTGG17600.043.803443
ATGAGTG16100.043.8016932