FastQCFastQC Report
Thu 26 May 2016
SRR2132768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703039
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2259326.101258992951466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2062235.569020472104128No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG217760.588057538686468No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT182190.4920012994732164No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162720.43942286322126234No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80680.21787510204456395No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73220.19772948651094413No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC69940.18887189683932576No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62630.16913135400410312No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51840.13999312456606589No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA51650.13948003248142946No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51360.1386968919311949No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47650.1286780938575046No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA46110.12451934748729354No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41740.11271822954065566No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41090.11096291451426787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT48650.085.254271
AACGTGT20400.071.310161
CGTGTGG23100.069.191093
GTGGAAT809300.067.934076
ACGTGTG22650.067.661932
AACGAGT693050.067.358911
TGGAATC818300.067.090777
GGAATCA820700.066.9434058
GAATCAC822500.066.83699
ATCGAGT15150.066.811141
ACGAGTG762000.066.76482
CGAGTGG786350.066.417333
GAGTGGA817400.065.3122564
AGTGGAA817150.065.297725
TCGAGTG17800.060.479822
ATGAGTG30450.045.5438462
GTGTGGA37750.044.9527174
TGAGTGG32950.043.0858653
AGCGAGT9350.042.7986831
AATGAGT30650.041.9329381
Produced by FastQC (version 0.11.5)