##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132768_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3703039
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.914782155953528	31.0	25.0	33.0	16.0	34.0
2	28.224317648288338	31.0	25.0	34.0	16.0	34.0
3	29.306336498211333	31.0	28.0	34.0	16.0	34.0
4	34.77474960431149	35.0	35.0	37.0	32.0	37.0
5	33.91949180119356	35.0	35.0	37.0	28.0	37.0
6	35.279824220052774	37.0	35.0	37.0	32.0	37.0
7	35.675361507129686	37.0	35.0	37.0	33.0	37.0
8	35.92929023971932	37.0	35.0	37.0	35.0	37.0
9	37.89267328807501	39.0	38.0	39.0	35.0	39.0
10-11	37.88695109611322	39.0	38.0	39.0	35.0	39.0
12-13	37.84189269948278	39.0	38.0	39.0	35.0	39.0
14-15	39.24345962869956	41.0	39.0	41.0	36.0	41.0
16-17	39.13153196604195	40.5	39.0	41.0	36.0	41.0
18-19	38.78288981023424	40.0	38.0	41.0	35.5	41.0
20-21	38.61469417416343	40.0	38.0	41.0	35.0	41.0
22-23	38.41722231388867	40.0	38.0	41.0	35.0	41.0
24-25	38.176364737179384	40.0	38.0	41.0	34.0	41.0
26-27	37.93383920612232	40.0	38.0	41.0	34.0	41.0
28-29	37.781103574658545	40.0	38.0	41.0	34.0	41.0
30-31	37.66555874782847	40.0	37.5	41.0	33.5	41.0
32-33	37.521301422966374	40.0	37.0	41.0	33.0	41.0
34-35	37.42924379138324	40.0	37.0	41.0	33.0	41.0
36-37	37.26960842162343	40.0	37.0	41.0	33.0	41.0
38-39	36.84254000565481	40.0	35.5	41.0	33.0	41.0
40-41	36.615726704471655	40.0	35.0	41.0	32.0	41.0
42-43	36.46453804564305	39.0	35.0	41.0	31.5	41.0
44-45	36.33595163323962	39.0	35.0	41.0	31.0	41.0
46-47	36.20222174273617	39.0	35.0	41.0	31.0	41.0
48-49	36.04343013940712	39.0	35.0	41.0	31.0	41.0
50-51	35.78117716286542	39.0	35.0	41.0	30.0	41.0
52-53	35.382923458273055	39.0	35.0	41.0	28.5	41.0
54-55	34.63028555734898	38.5	35.0	40.5	24.0	41.0
56-57	34.0190377417035	38.0	35.0	40.0	17.0	41.0
58-59	33.70861541020767	38.0	34.5	40.0	10.0	41.0
60-61	33.47168987418172	37.0	34.0	40.0	7.5	41.0
62-63	33.217990277715145	37.0	34.0	40.0	6.0	41.0
64-65	32.95296552372254	36.0	34.0	40.0	2.0	41.0
66-67	32.65085676926438	36.0	34.0	39.5	2.0	41.0
68-69	32.35227984366354	35.0	33.5	39.0	2.0	41.0
70-71	32.04416669659704	35.0	33.0	39.0	2.0	40.5
72-73	31.74838841826943	35.0	33.0	37.5	2.0	40.0
74-75	31.443297653629898	35.0	33.0	37.0	2.0	40.0
76-77	31.136584842881753	35.0	33.0	37.0	2.0	39.0
78-79	30.825279587927646	35.0	33.0	36.0	2.0	39.0
80-81	30.536577119495636	35.0	33.0	36.0	2.0	38.5
82-83	30.26237598361778	35.0	32.5	35.5	2.0	37.0
84-85	29.99612547963983	35.0	32.0	35.0	2.0	37.0
86-87	29.811749079607317	35.0	32.0	35.0	2.0	36.5
88-89	29.614839730286395	35.0	32.0	35.0	2.0	36.0
90-91	29.433942094587714	35.0	32.0	35.0	2.0	36.0
92-93	29.279347314462527	35.0	32.0	35.0	2.0	36.0
94-95	29.107338459033244	35.0	31.5	35.0	2.0	35.0
96-97	28.92654978789043	35.0	31.0	35.0	2.0	35.0
98-99	28.77133983736061	35.0	31.0	35.0	2.0	35.0
100	28.678338791462902	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1773474624615723
1101	2	2.234268358149613
1101	3	1.2532820278289876
1101	4	0.7090675577579617
1101	5	2.2485767167357196
1101	6	1.0062388091412942
1101	7	0.5727446073447524
1101	8	0.4062459992178944
1101	9	0.31834171842942993
1101	10-11	0.4746085116318213
1101	12-13	0.3879295447743729
1101	14-15	0.457654583705299
1101	16-17	0.45394811563375015
1101	18-19	0.41133666743905906
1101	20-21	0.3952605037989514
1101	22-23	0.3450512979605378
1101	24-25	0.2816949148053354
1101	26-27	0.190567094067994
1101	28-29	0.23136457002422617
1101	30-31	0.2587537205492296
1101	32-33	0.31537257389052087
1101	34-35	0.2888013455950613
1101	36-37	0.3211374019140578
1101	38-39	0.35045742082432696
1101	40-41	0.32451363482554285
1101	42-43	0.4423623053126846
1101	44-45	0.44497617044103777
1101	46-47	0.41746284245019893
1101	48-49	0.3833373542082299
1101	50-51	0.4041757440805469
1101	52-53	0.4286305574262599
1101	54-55	0.5045587659433011
1101	56-57	0.4483597646288615
1101	58-59	0.39524060159563135
1101	60-61	0.33605030558100424
1101	62-63	0.43227742878005415
1101	64-65	0.35081083617568964
1101	66-67	0.4016409007127706
1101	68-69	0.5245267107168345
1101	70-71	0.39561073794286017
1101	72-73	0.44024236710829
1101	74-75	0.4664603362372972
1101	76-77	0.37596922078214234
1101	78-79	0.4194538790478255
1101	80-81	0.4714510017240592
1101	82-83	0.4229775918388654
1101	84-85	0.43352216133213517
1101	86-87	0.51939385886946
1101	88-89	0.5245077221921584
1101	90-91	0.5105308374325581
1101	92-93	0.516602517869007
1101	94-95	0.5474547743432829
1101	96-97	0.5520985529999685
1101	98-99	0.5495136661258826
1101	100	0.5633143966751604
1102	1	1.684625224469503
1102	2	3.0988643987978364
1102	3	3.2181956112810752
1102	4	1.436130191174371
1102	5	2.6046891143995268
1102	6	1.2031135915557911
1102	7	0.7184101551225197
1102	8	0.5027316160329036
1102	9	0.4375094924070453
1102	10-11	0.449950741286159
1102	12-13	0.43903417873465855
1102	14-15	0.5616148720046041
1102	16-17	0.4606129466780544
1102	18-19	0.3562327570499235
1102	20-21	0.2269217783138302
1102	22-23	0.13217452766758697
1102	24-25	0.1236726654978213
1102	26-27	0.1835968152134697
1102	28-29	0.24536221046284368
1102	30-31	0.2498228341790778
1102	32-33	0.22725940114673193
1102	34-35	0.16371072293728872
1102	36-37	0.20957581185713536
1102	38-39	0.2043190082818569
1102	40-41	0.19681588387444293
1102	42-43	0.21239748535501235
1102	44-45	0.24893409614590922
1102	46-47	0.20640030790091402
1102	48-49	0.15168527465552017
1102	50-51	0.23917438279512737
1102	52-53	0.27582108808385897
1102	54-55	0.23425233983805782
1102	56-57	0.14842153529922086
1102	58-59	0.18015379447467694
1102	60-61	0.2163629470656261
1102	62-63	0.19880023171421612
1102	64-65	0.18629036130165133
1102	66-67	0.2321543347892998
1102	68-69	0.2073807189554202
1102	70-71	0.21978949226440392
1102	72-73	0.16432162640513326
1102	74-75	0.19209825602716535
1102	76-77	0.193595620050651
1102	78-79	0.22167177256660153
1102	80-81	0.21591947770351183
1102	82-83	0.24277651376679898
1102	84-85	0.18055069995652318
1102	86-87	0.18698328539728237
1102	88-89	0.20178065977158965
1102	90-91	0.22179661363702863
1102	92-93	0.21440992645968038
1102	94-95	0.19524148229646343
1102	96-97	0.20383535657304463
1102	98-99	0.27380868510366696
1102	100	0.26340583280506635
1103	1	1.2819936925520246
1103	2	2.8483365299470513
1103	3	3.1966599612410747
1103	4	1.4027004120796747
1103	5	2.588884962015051
1103	6	1.2412694257116286
1103	7	0.7252798285004758
1103	8	0.4736311478543911
1103	9	0.42267468247404594
1103	10-11	0.45907766928654326
1103	12-13	0.4251839912641131
1103	14-15	0.5352979188770988
1103	16-17	0.33342962841622636
1103	18-19	0.19643561483323424
1103	20-21	0.12708846702183507
1103	22-23	0.04957085209093748
1103	24-25	0.12473322846602031
1103	26-27	0.1858625587308893
1103	28-29	0.19974863313271385
1103	30-31	0.21688325366580585
1103	32-33	0.29344349859976404
1103	34-35	0.3207041682338314
1103	36-37	0.25591961287028653
1103	38-39	0.3276304713282343
1103	40-41	0.37311732721637014
1103	42-43	0.4139241971630341
1103	44-45	0.4843712372188378
1103	46-47	0.49679164468359005
1103	48-49	0.5016066151503296
1103	50-51	0.5221670412413104
1103	52-53	0.582073625178424
1103	54-55	0.642805571719947
1103	56-57	0.5756048025000098
1103	58-59	0.5913355329084595
1103	60-61	0.6912978442619178
1103	62-63	0.6233852204884514
1103	64-65	0.6141221034884197
1103	66-67	0.6941336846905699
1103	68-69	0.6732683944074722
1103	70-71	0.6102588375242348
1103	72-73	0.6274022221442905
1103	74-75	0.6823076623866129
1103	76-77	0.7359476618022285
1103	78-79	0.7151173116458978
1103	80-81	0.6697268683780315
1103	82-83	0.6928698660812174
1103	84-85	0.7076380490265208
1103	86-87	0.7513779365509272
1103	88-89	0.7365503949844445
1103	90-91	0.7429053807635313
1103	92-93	0.7562816116419278
1103	94-95	0.7580528332638927
1103	96-97	0.7868631194722084
1103	98-99	0.7812364937535037
1103	100	0.8142571732335
1104	1	6.041358014065366
1104	2	5.077506759678457
1104	3	4.101881036049036
1104	4	1.828908260432378
1104	5	2.8014013170328695
1104	6	1.2881445343167783
1104	7	0.6702063832734524
1104	8	0.5121994003479671
1104	9	0.4399712794066204
1104	10-11	0.45295537364656013
1104	12-13	0.36063742357844575
1104	14-15	0.4333826991777485
1104	16-17	0.24158712950166716
1104	18-19	0.09735273178807802
1104	20-21	0.005520279630168545
1104	22-23	-0.037874625064411305
1104	24-25	-0.0381901847729651
1104	26-27	0.0921336464858129
1104	28-29	0.04976094375592055
1104	30-31	0.13175026467096274
1104	32-33	0.183536481740326
1104	34-35	0.1801971761424852
1104	36-37	0.13804179788598248
1104	38-39	0.2086361954009135
1104	40-41	0.2614295162997138
1104	42-43	0.2975283927604835
1104	44-45	0.2989241804857059
1104	46-47	0.21581951172495195
1104	48-49	0.23184204419390397
1104	50-51	0.2922713291072512
1104	52-53	0.1831566785654104
1104	54-55	0.3663024152565768
1104	56-57	0.3883560750464454
1104	58-59	0.4254106404367377
1104	60-61	0.4119843781043855
1104	62-63	0.4873221675811621
1104	64-65	0.44282530048139535
1104	66-67	0.43970232534813647
1104	68-69	0.420436694087698
1104	70-71	0.41780222696431224
1104	72-73	0.4103929669051851
1104	74-75	0.4749203094838741
1104	76-77	0.499117348691442
1104	78-79	0.46707038100488063
1104	80-81	0.42782140906941635
1104	82-83	0.41392630600562086
1104	84-85	0.4587815052327642
1104	86-87	0.4821336546273791
1104	88-89	0.45913761073264325
1104	90-91	0.47768437203644964
1104	92-93	0.4950913163289208
1104	94-95	0.5270041810935737
1104	96-97	0.5388808012472488
1104	98-99	0.5115637629831618
1104	100	0.5541435679634752
1105	1	1.6949135061952951
1105	2	3.0378760149588686
1105	3	3.220067023021329
1105	4	1.4225748216597083
1105	5	2.572116078393364
1105	6	1.22281846820173
1105	7	0.7621316503318454
1105	8	0.5043581082601278
1105	9	0.4054123180151379
1105	10-11	0.460796243236679
1105	12-13	0.3565678772671177
1105	14-15	0.44924869564875536
1105	16-17	0.33408106641797986
1105	18-19	0.2599054214785639
1105	20-21	0.18730989401819187
1105	22-23	0.09200162028383119
1105	24-25	0.15077015746660294
1105	26-27	0.2367975896275638
1105	28-29	0.2606921572162122
1105	30-31	0.26713253650252256
1105	32-33	0.2744706657217151
1105	34-35	0.315135668441755
1105	36-37	0.28810219574556584
1105	38-39	0.29089577693696356
1105	40-41	0.3044735235172311
1105	42-43	0.35364615731201354
1105	44-45	0.39077928878008095
1105	46-47	0.4131252461874979
1105	48-49	0.3706250605195578
1105	50-51	0.3729610056917636
1105	52-53	0.3834236995378575
1105	54-55	0.3725466780393418
1105	56-57	0.4528855277486912
1105	58-59	0.484324072392468
1105	60-61	0.4841551849026402
1105	62-63	0.49291099154596907
1105	64-65	0.5051356623803898
1105	66-67	0.5228978479732191
1105	68-69	0.5105196779510379
1105	70-71	0.5546246124482295
1105	72-73	0.5582560265583325
1105	74-75	0.5387611754042965
1105	76-77	0.5190771066287354
1105	78-79	0.5805722685556844
1105	80-81	0.5294634235172921
1105	82-83	0.5915006606548374
1105	84-85	0.5796443894648675
1105	86-87	0.6180073395911592
1105	88-89	0.5814744446486912
1105	90-91	0.584599351154683
1105	92-93	0.6531845184565128
1105	94-95	0.6791271886727941
1105	96-97	0.6436708341252988
1105	98-99	0.6188989945464343
1105	100	0.6484943823531779
1106	1	-0.6424712527548131
1106	2	1.8348935529605406
1106	3	2.7437624906265192
1106	4	1.2264124604107707
1106	5	2.4409724275225315
1106	6	1.1196382116145358
1106	7	0.6219018379940877
1106	8	0.43356509245257513
1106	9	0.31801774695377816
1106	10-11	0.418056858964718
1106	12-13	0.3728047002363226
1106	14-15	0.4024822207722494
1106	16-17	0.347521925845669
1106	18-19	0.25277902176380707
1106	20-21	0.2733979653808447
1106	22-23	0.23137409329905267
1106	24-25	0.15935966012826697
1106	26-27	0.14138942825514533
1106	28-29	0.17485117043609932
1106	30-31	0.20796755595686278
1106	32-33	0.17426877543068287
1106	34-35	0.3034780992131587
1106	36-37	0.3079249792530945
1106	38-39	0.2798829268789049
1106	40-41	0.24141315921355044
1106	42-43	0.22116896241595185
1106	44-45	0.32617305319802625
1106	46-47	0.30308576244709684
1106	48-49	0.32808504272693284
1106	50-51	0.33106690307327824
1106	52-53	0.36506807074457015
1106	54-55	0.3406703016588608
1106	56-57	0.3108948563815872
1106	58-59	0.229257410568394
1106	60-61	0.267085728628345
1106	62-63	0.21873021496568867
1106	64-65	0.2836992063939121
1106	66-67	0.26755578801174806
1106	68-69	0.19175767153928547
1106	70-71	0.2296398724779678
1106	72-73	0.20870005144145765
1106	74-75	0.17642631665378516
1106	76-77	0.23106115555299667
1106	78-79	0.22600086450407275
1106	80-81	0.19916601110283239
1106	82-83	0.20515222174709535
1106	84-85	0.12668836158714214
1106	86-87	0.14900789961374628
1106	88-89	0.24564424630931114
1106	90-91	0.3035312047240062
1106	92-93	0.2666472818360326
1106	94-95	0.24734352453950947
1106	96-97	0.24800643019982616
1106	98-99	0.2661216935428854
1106	100	0.20978898157188297
1107	1	-0.8588494920382317
1107	2	1.7346634003493904
1107	3	-0.5525305545413381
1107	4	-0.04106301425852621
1107	5	1.8125123629292048
1107	6	0.7159405463703621
1107	7	0.33841041797212057
1107	8	0.2742505003514282
1107	9	0.2441501763733669
1107	10-11	0.2903542845620777
1107	12-13	0.28795188495916335
1107	14-15	0.36765127899628425
1107	16-17	0.2929655466405947
1107	18-19	0.2655982439265543
1107	20-21	0.19696593651846683
1107	22-23	0.19304533519010647
1107	24-25	0.04642343826508011
1107	26-27	0.05863014644260289
1107	28-29	-0.027797201062298882
1107	30-31	-0.010411832452675185
1107	32-33	-0.010758127543461171
1107	34-35	0.024065606313349974
1107	36-37	0.06467537907369092
1107	38-39	0.07291315773539964
1107	40-41	0.06498771900166389
1107	42-43	0.12218390105556409
1107	44-45	0.15546551807356224
1107	46-47	0.15307559300828188
1107	48-49	0.13698768047883192
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2216	82-83	0.057889188614844045
2216	84-85	0.09813252714875631
2216	86-87	0.023609988819739414
2216	88-89	0.13569702216532775
2216	90-91	0.11277409644558034
2216	92-93	0.10946726325372325
2216	94-95	0.03158785379277873
2216	96-97	0.03736016142576304
2216	98-99	-0.06576388873677885
2216	100	-0.14579893402636301
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1750.0
3	450.0
4	686.0
5	2527.0
6	8164.0
7	14693.0
8	17208.0
9	23283.0
10	15055.0
11	8020.0
12	7545.0
13	9734.0
14	10236.0
15	11514.0
16	13234.0
17	15096.0
18	20548.0
19	34451.0
20	67244.0
21	77468.0
22	35617.0
23	23301.0
24	23329.0
25	24740.0
26	26436.0
27	29258.0
28	35427.0
29	42025.0
30	49513.0
31	62193.0
32	82057.0
33	118255.0
34	175940.0
35	414101.0
36	461000.0
37	747585.0
38	779654.0
39	212705.0
40	997.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.041170274761454	41.78369819913005	25.61529610444748	19.55983542166101
2	14.6917095416119	40.94219464446451	25.3646474595194	19.001448354404193
3	18.338536024959957	27.936415087562665	19.304294941439	34.42075394603838
4	29.746162543791733	29.81008301559881	21.678167580735714	18.765586859873743
5	16.71422321602178	39.365048501739146	22.37266943202195	21.548058850217124
6	34.06217725172961	25.86091413113465	24.163663352217483	15.913245264918263
7	18.995598339797148	25.41444983568378	37.45254084545687	18.137410979062203
8	33.10680767877411	25.80218571827086	23.59586274948765	17.495143853467383
9	32.42101960038768	27.95933286146865	23.455464552223187	16.164182985920483
10-11	16.362452569362624	43.24672248928515	23.47943135354502	16.911393587807204
12-13	16.209524123294408	27.631075449110853	30.972520138189203	25.18688028940554
14-15	16.469823839284437	35.041259354816404	23.39134154406691	25.09757526183224
16-17	24.644190892939555	27.051659461323524	31.118521841114827	17.185627804622094
18-19	16.841207991598253	27.25183288644813	31.378632523178933	24.528326598774683
20-21	24.71386339706387	35.33273616615974	23.430079996456964	16.523320440319424
22-23	24.161303729180275	27.60852640223341	31.404786717072113	16.825383151514202
24-25	16.63422934514057	35.322285290541096	23.079192522682046	24.964292841636286
26-27	17.336270201999064	30.76181793484645	34.77858849371475	17.12332336943973
28-29	24.419254023519603	27.40397819196611	31.149631424351725	17.027136360162558
30-31	22.264078234120678	28.62430020315746	31.372704959359055	17.738916603362807
32-33	18.964531564479877	29.925677261298084	32.10383417511941	19.00595699910263
34-35	18.963127312458766	29.79562192026603	32.268388207631624	18.97286255964358
36-37	19.160559745657554	29.6849020493708	32.611673817099955	18.542864387871692
38-39	19.60522154911142	29.308049415628624	32.32792039187273	18.75880864338723
40-41	18.525716310306212	30.53481208272449	32.305384847418566	18.634086759550737
42-43	18.485886871368827	29.692890046626985	32.796031116341695	19.02519196566249
44-45	18.667640281401304	30.19629282867396	31.979409344595073	19.15665754532966
46-47	19.303968983313435	29.40514534143443	32.42768979748796	18.863195877764184
48-49	18.73717776129282	29.79605399781099	32.359867125352984	19.106901115543206
50-51	19.098084033141426	30.40225879338565	31.675942381379187	18.82371479209374
52-53	19.287918368482245	30.030295456653676	31.63433301454039	19.047453160323684
54-55	19.470480867201235	30.842208251114826	29.984696353454552	19.702614528229383
56-57	19.749819000015933	31.430522335843612	28.92628189981256	19.893376764327893
58-59	20.67723294299628	31.730748717472324	27.843576586690013	19.748441752841384
60-61	20.2313019117541	31.970551754923456	27.679319607489955	20.118826725832484
62-63	19.450232484786817	32.12027529828212	27.54134578149077	20.888146435440305
64-65	19.88801630228577	32.068781884284775	28.182595430401896	19.860606383027562
66-67	20.076445329963487	31.394893679879377	28.50596881452893	20.022692175628205
68-69	20.280950322154318	31.684610937124884	28.146773501440304	19.887665239280494
70-71	18.93358401032233	32.53736998179063	28.46894132089886	20.060104686988172
72-73	18.94694643279819	31.976171350881977	28.649752641187742	20.427129575132092
74-75	19.341111480778952	32.15366684093865	27.957231711163633	20.547989967118767
76-77	20.13413113303797	31.62143689282634	28.083741423887222	20.16069055024847
78-79	19.927121666336756	31.962619169591534	27.547332299762818	20.5629268643089
80-81	20.122908585372492	32.45278957903276	27.29784207166305	20.126459763931692
82-83	19.854784975876935	32.560525726294536	27.30750259621174	20.277186701616788
84-85	19.969945884228057	32.76638562328196	26.686334572603144	20.577333919886836
86-87	19.903503516641006	32.96774147378779	26.857445234471765	20.271309775099436
88-89	20.99340568651856	32.288439306202285	26.65413461753981	20.064020389739344
90-91	20.27727785021833	33.01790301013776	26.68438455845628	20.02043458118763
92-93	19.709136198673576	33.13277283874137	26.594453906642624	20.56363705594243
94-95	19.91290395711522	33.328575978888686	26.446133357781726	20.31238670621437
96-97	19.814952316255656	32.600763463810274	26.84202758769498	20.742256632239098
98-99	20.53992840147993	32.670102520665964	25.996787207810296	20.79318187004381
100	19.940891791849882	33.450579375480515	25.797270836196972	20.811257996472627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	227321.0
1	121766.0
2	11375.0
3	5353.5
4	3804.0
5	3241.0
6	3205.0
7	3587.0
8	3441.0
9	2964.0
10	2960.0
11	4497.5
12	30513.5
13	33654.0
14	10644.5
15	9209.0
16	9906.0
17	11121.5
18	12388.0
19	13764.0
20	15402.5
21	17141.0
22	19352.5
23	22451.0
24	25779.5
25	29512.0
26	34641.5
27	39821.0
28	43406.0
29	46812.0
30	50509.5
31	54630.5
32	60093.0
33	65697.5
34	72086.5
35	77715.5
36	82752.5
37	87617.0
38	90236.5
39	92327.5
40	94081.5
41	96009.0
42	96987.5
43	97180.5
44	98995.0
45	101569.0
46	104874.0
47	109647.5
48	113591.5
49	120804.5
50	147109.0
51	149584.0
52	120330.0
53	106269.0
54	102948.5
55	102748.0
56	94496.5
57	80597.5
58	70767.0
59	58296.0
60	45543.5
61	36850.5
62	28976.0
63	21856.0
64	16290.5
65	11930.0
66	9008.5
67	7093.0
68	5594.0
69	4189.0
70	2895.0
71	2104.0
72	1580.0
73	1043.0
74	713.0
75	508.0
76	363.0
77	227.5
78	151.5
79	95.0
80	42.0
81	21.5
82	15.5
83	9.0
84	5.0
85	2.0
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13783273684128092
2	0.005833046856919411
3	0.003996717290852189
4	0.0
5	0.002133382878225155
6	0.012503243957192997
7	0.003186571894057827
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.400969311962418E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.91159936473799E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	2.2954119575840278E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.700484655981209E-5
64-65	0.0
66-67	1.3502423279906045E-5
68-69	0.0
70-71	0.0
72-73	4.725848147967116E-4
74-75	4.0507269839718137E-4
76-77	5.671017777560539E-4
78-79	4.8608723807661757E-4
80-81	0.0011612084020719198
82-83	0.0012827302115910742
84-85	0.0014042520211102287
86-87	6.751211639953023E-5
88-89	0.0
90-91	6.751211639953023E-5
92-93	0.0
94-95	1.3502423279906045E-5
96-97	0.0012827302115910742
98-99	7.966429735144567E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3703039.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.801730300588105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.41165581753813	48.213380334908194
2	11.3259511563452	13.093191482733985
3	2.8787126726996903	4.99183720560818
4	0.9070210701391914	2.097095490925454
5	0.40696162823050175	1.176154313883383
6	0.23447541351566165	0.8131850768490686
7	0.14455493156648322	0.5848867617618083
8	0.10191240827889499	0.47125708300960883
9	0.07567820538323165	0.3936898095474664
>10	0.40659263706690146	5.25908759362534
>50	0.0755539561677888	2.840296997036844
>100	0.02643776498402488	2.864192170894322
>500	0.0021057834782897034	0.8562450553946487
>1k	0.0018250123457195842	1.940751797949415
>5k	3.275663184624895E-4	1.1937012494195705
>10k+	2.3397594175892107E-4	13.211047576452584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	225932	6.101258992951466	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	206223	5.569020472104128	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	21776	0.588057538686468	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18219	0.4920012994732164	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16272	0.43942286322126234	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8068	0.21787510204456395	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7322	0.19772948651094413	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6994	0.18887189683932576	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6263	0.16913135400410312	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5184	0.13999312456606589	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5165	0.13948003248142946	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5136	0.1386968919311949	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4765	0.1286780938575046	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	4611	0.12451934748729354	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4174	0.11271822954065566	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4109	0.11096291451426787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018633344126270342	0.0	0.0	0.0	0.0
2	0.0018633344126270342	0.0	0.0	0.0	0.0
3	0.0018633344126270342	0.0	0.0	0.0	0.0
4	0.0018633344126270342	0.0	0.0	0.0	0.0
5	0.0018633344126270342	0.0	0.0	0.0	0.0
6	0.0018633344126270342	0.0	0.0	2.700484655981209E-5	0.0
7	0.0018633344126270342	0.0	0.0	2.700484655981209E-5	0.0
8	0.0018633344126270342	0.0	0.0	2.700484655981209E-5	0.0
9	0.0018633344126270342	0.0	0.0	2.700484655981209E-5	0.0
10-11	0.0018633344126270342	0.0	0.0	2.700484655981209E-5	0.0
12-13	0.0019173441057466585	0.0	0.0	2.700484655981209E-5	0.0
14-15	0.0020118610687060007	0.0	0.0	2.700484655981209E-5	0.0
16-17	0.002065870761825625	0.0	0.0	2.700484655981209E-5	0.0
18-19	0.002160387724784967	0.0	0.0	2.700484655981209E-5	0.0
20-21	0.0022819095343041213	0.0	0.0	2.700484655981209E-5	0.0
22-23	0.002430436190383088	0.0	0.0	2.700484655981209E-5	0.0
24-25	0.002592465269741961	0.0	0.0	5.400969311962418E-5	0.0
26-27	0.002700484655981209	0.0	0.0	9.451696295934231E-5	0.0
28-29	0.002808504042220457	0.0	0.0	1.0801938623924836E-4	0.0
30-31	0.0030515476612587664	0.0	0.0	1.0801938623924836E-4	0.0
32-33	0.0032135767406176385	1.3502423279906045E-5	0.0	1.0801938623924836E-4	0.0
34-35	0.0034296155130961355	2.700484655981209E-5	0.0	1.0801938623924836E-4	0.0
36-37	0.0038211857882134107	2.700484655981209E-5	0.0	1.0801938623924836E-4	0.0
38-39	0.0048338675342063635	2.700484655981209E-5	0.0	1.0801938623924836E-4	0.0
40-41	0.006602684983874056	2.700484655981209E-5	0.0	1.0801938623924836E-4	0.0
42-43	0.008857589671618364	2.700484655981209E-5	0.0	1.0801938623924836E-4	0.0
44-45	0.01157157675087948	5.400969311962418E-5	0.0	1.7553150263877856E-4	0.0
46-47	0.014528607449178905	5.400969311962418E-5	0.0	1.8903392591868462E-4	0.0
48-49	0.017701676919956826	5.400969311962418E-5	0.0	1.8903392591868462E-4	0.0
50-51	0.022049457216086574	5.400969311962418E-5	0.0	2.1603877247849672E-4	0.0
52-53	0.027639460453967674	5.400969311962418E-5	0.0	2.700484655981209E-4	0.0
54-55	0.03557888534255243	5.400969311962418E-5	0.0	2.700484655981209E-4	0.0
56-57	0.04442297259089088	5.400969311962418E-5	0.0	2.8355088887802694E-4	0.0
58-59	0.05437425854818164	5.400969311962418E-5	0.0	3.2405815871774506E-4	0.0
60-61	0.06741759943657089	5.400969311962418E-5	0.0	3.375605819976511E-4	0.0
62-63	0.08240528927726659	5.400969311962418E-5	0.0	3.6456542855746324E-4	0.0
64-65	0.10186228122361121	5.400969311962418E-5	0.0	3.7806785183736925E-4	0.0
66-67	0.12580207769888463	6.751211639953022E-5	0.0	4.185751216770874E-4	1.3502423279906045E-5
68-69	0.15527786771891952	1.0801938623924836E-4	0.0	4.590823915168055E-4	2.700484655981209E-5
70-71	0.19394880799257042	1.0801938623924836E-4	0.0	4.590823915168055E-4	2.700484655981209E-5
72-73	0.23798021030834404	1.0801938623924836E-4	0.0	4.995896613565237E-4	2.700484655981209E-5
74-75	0.28923540907886736	1.0801938623924836E-4	0.0	5.265945079163357E-4	4.050726983971813E-5
76-77	0.3483760230448558	1.0801938623924836E-4	0.0	5.671017777560539E-4	5.400969311962418E-5
78-79	0.4198848567352383	1.0801938623924836E-4	0.0	5.94106624315866E-4	5.400969311962418E-5
80-81	0.5093384109646159	1.0801938623924836E-4	0.0	6.34613894155584E-4	5.400969311962418E-5
82-83	0.618100430484259	1.0801938623924836E-4	0.0	6.481163174354901E-4	5.400969311962418E-5
84-85	0.750734734362776	1.0801938623924836E-4	0.0	6.751211639953022E-4	5.400969311962418E-5
86-87	0.9014352805898075	1.0801938623924836E-4	0.0	6.751211639953022E-4	5.400969311962418E-5
88	1.0286686151563622	1.0801938623924836E-4	0.0	7.021260105551144E-4	5.400969311962418E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4865	0.0	85.25427	1
AACGTGT	2040	0.0	71.31016	1
CGTGTGG	2310	0.0	69.19109	3
GTGGAAT	80930	0.0	67.93407	6
ACGTGTG	2265	0.0	67.66193	2
AACGAGT	69305	0.0	67.35891	1
TGGAATC	81830	0.0	67.09077	7
GGAATCA	82070	0.0	66.943405	8
GAATCAC	82250	0.0	66.8369	9
ATCGAGT	1515	0.0	66.81114	1
ACGAGTG	76200	0.0	66.7648	2
CGAGTGG	78635	0.0	66.41733	3
GAGTGGA	81740	0.0	65.312256	4
AGTGGAA	81715	0.0	65.29772	5
TCGAGTG	1780	0.0	60.47982	2
ATGAGTG	3045	0.0	45.543846	2
GTGTGGA	3775	0.0	44.952717	4
TGAGTGG	3295	0.0	43.085865	3
AGCGAGT	935	0.0	42.798683	1
AATGAGT	3065	0.0	41.932938	1
>>END_MODULE