FastQCFastQC Report
Thu 26 May 2016
SRR2132767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609442
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3297927.154705493636756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1459883.1671512517133307No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT259860.5637558732705608No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG225010.48815019258296344No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117440.2547813813472433No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110260.23920465861160634No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90190.19566359659151802No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84290.18286378264440684No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76160.16522607291728586No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC60740.13177299985551397No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA58850.1276727204724563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT61700.088.205111
ATCGAGT21900.077.174071
AACGAGT893550.074.4043661
GTGGAAT1061600.074.189446
ACGAGTG977450.073.7142942
TGGAATC1066800.073.695247
CGAGTGG1012100.073.431023
GGAATCA1073300.073.297518
GAATCAC1076700.073.118429
TCGAGTG24000.073.065952
AACGTGT33200.072.744531
AGTGGAA1055150.072.028515
GAGTGGA1057450.071.9918754
CGTGTGG37650.071.2967453
ACGTGTG37450.068.291172
GCGAGTG12150.054.945172
GTGTGGA59000.047.8066644
TGAGTGG47250.044.0758323
ATGAGTG43250.043.806282
AGCGAGT14750.043.0886421