##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132767_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4609442
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.881347026386273	31.0	25.0	33.0	16.0	34.0
2	28.144415094061277	31.0	25.0	34.0	16.0	34.0
3	29.22291396659292	31.0	28.0	34.0	16.0	34.0
4	34.74206877969177	35.0	35.0	37.0	32.0	37.0
5	33.81497890634051	35.0	35.0	37.0	28.0	37.0
6	35.24971742783617	37.0	35.0	37.0	32.0	37.0
7	35.64718354195584	37.0	35.0	37.0	33.0	37.0
8	35.9202810231694	37.0	35.0	37.0	35.0	37.0
9	37.8889388346789	39.0	38.0	39.0	35.0	39.0
10-11	37.88253730061035	39.0	38.0	39.0	35.0	39.0
12-13	37.857840059599404	39.0	38.0	39.0	35.0	39.0
14-15	39.276449839264714	41.0	39.0	41.0	36.0	41.0
16-17	39.23514635827938	40.5	39.0	41.0	36.0	41.0
18-19	39.056597523084136	40.0	39.0	41.0	35.5	41.0
20-21	38.96855465368694	40.0	39.0	41.0	35.0	41.0
22-23	38.82245280448262	40.0	39.0	41.0	35.0	41.0
24-25	38.673602249469674	40.0	39.0	41.0	35.0	41.0
26-27	38.48220608915352	40.0	38.5	41.0	35.0	41.0
28-29	38.31901149857185	40.0	38.0	41.0	34.0	41.0
30-31	38.193931065842676	40.0	38.0	41.0	34.0	41.0
32-33	38.044407001975515	40.0	38.0	41.0	34.0	41.0
34-35	37.95855951761623	40.0	38.0	41.0	34.0	41.0
36-37	37.794100023386775	40.0	38.0	41.0	33.5	41.0
38-39	37.32137501675908	40.0	36.0	41.0	33.0	41.0
40-41	37.07549102472707	40.0	35.0	41.0	33.0	41.0
42-43	36.9214423351026	40.0	35.0	41.0	33.0	41.0
44-45	36.782511093533664	40.0	35.0	41.0	33.0	41.0
46-47	36.64819451031166	39.5	35.0	41.0	32.0	41.0
48-49	36.48651159945173	39.0	35.0	41.0	31.5	41.0
50-51	36.21249480956698	39.0	35.0	41.0	31.0	41.0
52-53	35.76956831217315	39.0	35.0	41.0	29.5	41.0
54-55	34.93256439716564	39.0	35.0	41.0	24.5	41.0
56-57	34.23720029452589	39.0	35.0	41.0	17.5	41.0
58-59	33.89989775335062	38.5	35.0	41.0	10.0	41.0
60-61	33.660391973692256	38.0	34.0	40.0	7.5	41.0
62-63	33.400417881383476	37.5	34.0	40.0	6.0	41.0
64-65	33.131936251719836	37.0	34.0	40.0	2.0	41.0
66-67	32.82949563092453	36.5	34.0	40.0	2.0	41.0
68-69	32.51855723534432	36.0	34.0	39.0	2.0	41.0
70-71	32.19663757565449	35.5	33.0	39.0	2.0	41.0
72-73	31.880338878328438	35.0	33.0	39.0	2.0	40.5
74-75	31.549956046740583	35.0	33.0	37.5	2.0	40.0
76-77	31.221109084353376	35.0	33.0	37.0	2.0	39.5
78-79	30.882085293621223	35.0	33.0	37.0	2.0	39.0
80-81	30.567774797903954	35.0	33.0	36.0	2.0	39.0
82-83	30.265731947597995	35.0	32.5	36.0	2.0	37.5
84-85	29.978329155676544	35.0	32.0	35.5	2.0	37.0
86-87	29.756648201669528	35.0	32.0	35.0	2.0	37.0
88-89	29.53358942362221	35.0	32.0	35.0	2.0	36.0
90-91	29.328251987984665	35.0	32.0	35.0	2.0	36.0
92-93	29.147747384607506	35.0	32.0	35.0	2.0	36.0
94-95	28.956093492444424	35.0	31.0	35.0	2.0	35.5
96-97	28.765409891262326	35.0	31.0	35.0	2.0	35.0
98-99	28.594047500760396	35.0	31.0	35.0	2.0	35.0
100	28.488074912321274	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3689612222992551
1101	2	2.339803366000986
1101	3	1.2804223576568212
1101	4	0.6766437291188723
1101	5	2.28148041432339
1101	6	1.0364817453590263
1101	7	0.5780545023604589
1101	8	0.40557143288506836
1101	9	0.32507492060933885
1101	10-11	0.47996217334816293
1101	12-13	0.42560296970417966
1101	14-15	0.5554900363240165
1101	16-17	0.4999215687102563
1101	18-19	0.476620480774983
1101	20-21	0.45167287451010196
1101	22-23	0.4555102343105659
1101	24-25	0.3735993171865246
1101	26-27	0.36063379324959755
1101	28-29	0.4342419199698284
1101	30-31	0.4406528907353078
1101	32-33	0.5208436054825896
1101	34-35	0.47625544095112815
1101	36-37	0.5620719086486261
1101	38-39	0.5478200896672902
1101	40-41	0.5123171702803404
1101	42-43	0.6130274844281232
1101	44-45	0.6179920106746337
1101	46-47	0.5784016578597786
1101	48-49	0.5665481920422053
1101	50-51	0.6151145050272646
1101	52-53	0.6924546171654171
1101	54-55	0.7297877606321848
1101	56-57	0.5721687615354369
1101	58-59	0.6000310278313634
1101	60-61	0.6227511189571757
1101	62-63	0.6683999131687486
1101	64-65	0.6253647771128712
1101	66-67	0.6209651372473175
1101	68-69	0.6700387636435678
1101	70-71	0.6059316735734726
1101	72-73	0.6514983075832568
1101	74-75	0.6425996662861841
1101	76-77	0.5492578568047009
1101	78-79	0.6048892769136209
1101	80-81	0.597307732411906
1101	82-83	0.6185746923057884
1101	84-85	0.6499705981271404
1101	86-87	0.6913257497896446
1101	88-89	0.6886524677417931
1101	90-91	0.645871155393376
1101	92-93	0.6432694510197372
1101	94-95	0.6547940907992782
1101	96-97	0.6728439172812593
1101	98-99	0.6770514505087384
1101	100	0.6928255854391736
1102	1	1.561100607443887
1102	2	3.0595217427294656
1102	3	3.2387529245058317
1102	4	1.4470101155370543
1102	5	2.6374067311276406
1102	6	1.2253412881715207
1102	7	0.7299577952499661
1102	8	0.5284930903981788
1102	9	0.42944028789818134
1102	10-11	0.5155838966045394
1102	12-13	0.510406734756458
1102	14-15	0.60001746221063
1102	16-17	0.533105436385803
1102	18-19	0.4416029595062696
1102	20-21	0.4221559606598717
1102	22-23	0.3590439982248341
1102	24-25	0.39948811067801415
1102	26-27	0.41722675727056213
1102	28-29	0.4697384628308967
1102	30-31	0.46989651157280576
1102	32-33	0.44827199280101837
1102	34-35	0.47268819834833664
1102	36-37	0.4251494817146195
1102	38-39	0.4942311380150457
1102	40-41	0.53292722175145
1102	42-43	0.5499606209182417
1102	44-45	0.5333111155804389
1102	46-47	0.5634729081198202
1102	48-49	0.5700583713756231
1102	50-51	0.6391104433692121
1102	52-53	0.6374959748979379
1102	54-55	0.6932338529670474
1102	56-57	0.6299551252805315
1102	58-59	0.696772287492621
1102	60-61	0.6411449627926373
1102	62-63	0.6319588454459293
1102	64-65	0.7137624680528418
1102	66-67	0.7034161523537747
1102	68-69	0.7073251342746687
1102	70-71	0.7062379922478215
1102	72-73	0.7089896964477838
1102	74-75	0.7286584608431887
1102	76-77	0.7301536848980206
1102	78-79	0.7804445972978762
1102	80-81	0.7898846056419302
1102	82-83	0.7808690180893976
1102	84-85	0.7792994333260985
1102	86-87	0.7567727623127602
1102	88-89	0.7693307341926499
1102	90-91	0.8111583853259177
1102	92-93	0.827231791074059
1102	94-95	0.8320428620090929
1102	96-97	0.8800018330385448
1102	98-99	0.9520537428248907
1102	100	0.9237270582477741
1103	1	1.496092799050949
1103	2	3.00449109937545
1103	3	3.209879754441509
1103	4	1.419479273705818
1103	5	2.6033693470560735
1103	6	1.2025900803107419
1103	7	0.6961983488116346
1103	8	0.4730312370255021
1103	9	0.3995773560607532
1103	10-11	0.4598173233391947
1103	12-13	0.47632743931654176
1103	14-15	0.5580656076408346
1103	16-17	0.42740643401366896
1103	18-19	0.42879897999273453
1103	20-21	0.3611576876550373
1103	22-23	0.35556177441510783
1103	24-25	0.3587552920970367
1103	26-27	0.4695707541722314
1103	28-29	0.47817610851699754
1103	30-31	0.5104649347015027
1103	32-33	0.4931355968888056
1103	34-35	0.5244576627768396
1103	36-37	0.4964416751569871
1103	38-39	0.5644400750933869
1103	40-41	0.6053880150263353
1103	42-43	0.5939125940877403
1103	44-45	0.6507057186127838
1103	46-47	0.5996982602726391
1103	48-49	0.7002506885065003
1103	50-51	0.6883380002529336
1103	52-53	0.7206515622021286
1103	54-55	0.6731157009700581
1103	56-57	0.6431890808474918
1103	58-59	0.7265861996267802
1103	60-61	0.6828147433387031
1103	62-63	0.6304470294815232
1103	64-65	0.6410766673011707
1103	66-67	0.6565665159876843
1103	68-69	0.6388962268003695
1103	70-71	0.6545021055774072
1103	72-73	0.6953096190006498
1103	74-75	0.709959759906166
1103	76-77	0.695488568810049
1103	78-79	0.6852630542476454
1103	80-81	0.6326882890038128
1103	82-83	0.6568028777616206
1103	84-85	0.7243603640959897
1103	86-87	0.7332003505913249
1103	88-89	0.7850330547146669
1103	90-91	0.76208073783787
1103	92-93	0.7946440554510055
1103	94-95	0.7632263243445117
1103	96-97	0.7305828777197689
1103	98-99	0.7452409356283809
1103	100	0.7826519846374005
1104	1	6.106808057527555
1104	2	5.127947052079939
1104	3	4.1274528371932675
1104	4	1.8625452833371412
1104	5	2.8428751672786277
1104	6	1.2987602016981228
1104	7	0.7113257805802675
1104	8	0.5420701503055909
1104	9	0.4688285096906952
1104	10-11	0.4870093810113829
1104	12-13	0.41250631790937575
1104	14-15	0.5129605677456297
1104	16-17	0.3952853116551083
1104	18-19	0.3125060391237682
1104	20-21	0.29686869774232605
1104	22-23	0.2701919842613023
1104	24-25	0.23995483096664572
1104	26-27	0.299735811282936
1104	28-29	0.29737845503084515
1104	30-31	0.4061776529753587
1104	32-33	0.38948293680225987
1104	34-35	0.40598808365204775
1104	36-37	0.4200368570644457
1104	38-39	0.41733132833999775
1104	40-41	0.4308919404814162
1104	42-43	0.535011081691124
1104	44-45	0.4989208777423144
1104	46-47	0.458422890715255
1104	48-49	0.5872770147575039
1104	50-51	0.5693415275449141
1104	52-53	0.4747006700337053
1104	54-55	0.5173838694013213
1104	56-57	0.4934678849895846
1104	58-59	0.45632361525944987
1104	60-61	0.4087200443205248
1104	62-63	0.41683766439357584
1104	64-65	0.36689693384858657
1104	66-67	0.41990742069308595
1104	68-69	0.44319830270192995
1104	70-71	0.4592682260592511
1104	72-73	0.4107110554269404
1104	74-75	0.4680875880800599
1104	76-77	0.46470833792727007
1104	78-79	0.479505990603716
1104	80-81	0.4435709487656183
1104	82-83	0.38939225018516765
1104	84-85	0.3606127287022538
1104	86-87	0.3516437370775556
1104	88-89	0.35291864213698787
1104	90-91	0.3641856745787351
1104	92-93	0.3967103697133787
1104	94-95	0.4345562887246892
1104	96-97	0.40854762739353845
1104	98-99	0.4364175554393803
1104	100	0.44736738304134605
1105	1	1.7078759111834003
1105	2	3.0632090264931087
1105	3	3.2422003635315946
1105	4	1.4025537394484715
1105	5	2.5664357329550427
1105	6	1.1975081183588472
1105	7	0.7238272748549264
1105	8	0.4912164314931573
1105	9	0.3725500050188373
1105	10-11	0.4381770589336398
1105	12-13	0.3830037229766745
1105	14-15	0.4624762834504992
1105	16-17	0.34729706833110896
1105	18-19	0.3182080387853006
1105	20-21	0.22832113631169193
1105	22-23	0.2125034277779747
1105	24-25	0.2594592821268904
1105	26-27	0.2835418647743424
1105	28-29	0.34535077636058276
1105	30-31	0.3740046536161401
1105	32-33	0.3991744244403179
1105	34-35	0.3949064985667974
1105	36-37	0.40495402483552567
1105	38-39	0.40211940749782826
1105	40-41	0.40628101157867036
1105	42-43	0.3723415403137267
1105	44-45	0.48562475057770627
1105	46-47	0.4087848649219197
1105	48-49	0.3824663140760194
1105	50-51	0.4361670749001334
1105	52-53	0.502843839688282
1105	54-55	0.476051519544086
1105	56-57	0.5001238522072242
1105	58-59	0.5381577654083003
1105	60-61	0.47321878929371053
1105	62-63	0.5032488593435929
1105	64-65	0.4855053594172247
1105	66-67	0.41669253814692553
1105	68-69	0.44746542994117533
1105	70-71	0.4905299432293617
1105	72-73	0.5095551727278043
1105	74-75	0.46371996298308815
1105	76-77	0.37295510494298156
1105	78-79	0.4027197354197263
1105	80-81	0.3510024982029769
1105	82-83	0.3594254180838199
1105	84-85	0.3470627346400157
1105	86-87	0.42879944316792873
1105	88-89	0.3401708691101888
1105	90-91	0.3472715835630069
1105	92-93	0.38876039424291875
1105	94-95	0.40981013349706075
1105	96-97	0.393283966215936
1105	98-99	0.4257294066836721
1105	100	0.43890350328582173
1106	1	-0.8481078945582077
1106	2	1.7576006009846168
1106	3	2.649538421029977
1106	4	1.165092611500313
1106	5	2.4122528731330988
1106	6	1.092093966259803
1106	7	0.6222474854274367
1106	8	0.40000951444040567
1106	9	0.31227616769487554
1106	10-11	0.4159885053248331
1106	12-13	0.36487064901701416
1106	14-15	0.3834162373982366
1106	16-17	0.2913108999471845
1106	18-19	0.23936917556825676
1106	20-21	0.24600735805017138
1106	22-23	0.2524144495158964
1106	24-25	0.22723279156220144
1106	26-27	0.16707916575959558
1106	28-29	0.22856278183690648
1106	30-31	0.22100133679562362
1106	32-33	0.15002037387200318
1106	34-35	0.2465679514498902
1106	36-37	0.24410583822029963
1106	38-39	0.2691396571700082
1106	40-41	0.22300780131809006
1106	42-43	0.24716009843938025
1106	44-45	0.32313611636903516
1106	46-47	0.24820729552205734
1106	48-49	0.30335816726054077
1106	50-51	0.36629882703139316
1106	52-53	0.3634111945704248
1106	54-55	0.32527947553973746
1106	56-57	0.3160912047382567
1106	58-59	0.2980351167766173
1106	60-61	0.29558602122625643
1106	62-63	0.31150145205129576
1106	64-65	0.29098324687978305
1106	66-67	0.3137623875018889
1106	68-69	0.3013293346970869
1106	70-71	0.3261425101946358
1106	72-73	0.3520842227733745
1106	74-75	0.3481433206939535
1106	76-77	0.3499004577012883
1106	78-79	0.3839271714305639
1106	80-81	0.3391216261518615
1106	82-83	0.33344937596839586
1106	84-85	0.3340340958917807
1106	86-87	0.3170216004325823
1106	88-89	0.29914451555569954
1106	90-91	0.31179809802414127
1106	92-93	0.2972574553957763
1106	94-95	0.2966256377387708
1106	96-97	0.35519835321266413
1106	98-99	0.3349166228228988
1106	100	0.3634174929437357
1107	1	-1.0221998562488537
1107	2	1.6791968964627983
1107	3	-0.7071676631053343
1107	4	-0.10757910178152486
1107	5	1.7959923901448818
1107	6	0.7544738861718159
1107	7	0.32998829051177125
1107	8	0.2477482347514126
1107	9	0.23513717777917975
1107	10-11	0.2893670049850101
1107	12-13	0.2537459808374578
1107	14-15	0.26514097796348324
1107	16-17	0.19557283188023433
1107	18-19	0.17898941543415958
1107	20-21	0.20696878054408785
1107	22-23	0.17511717790485193
1107	24-25	0.051302759851829194
1107	26-27	0.09475805930035364
1107	28-29	0.02939185371367614
1107	30-31	0.06103191039316869
1107	32-33	0.05395732090135397
1107	34-35	0.05763156408779224
1107	36-37	0.0772169205586195
1107	38-39	0.08693538454061667
1107	40-41	0.09822587575564512
1107	42-43	0.09987397503059015
1107	44-45	0.10627763414078828
1107	46-47	0.10149236443773901
1107	48-49	0.08324636479308367
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2216	88-89	0.07467589501829153
2216	90-91	0.12736979144820282
2216	92-93	0.09263220406255002
2216	94-95	0.011297939317426398
2216	96-97	0.024923576156101745
2216	98-99	0.004463370241360565
2216	100	-0.011355452634816743
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2126.0
3	555.0
4	718.0
5	1922.0
6	5707.0
7	9721.0
8	11792.0
9	16345.0
10	11652.0
11	9580.0
12	8615.0
13	12310.0
14	12526.0
15	13820.0
16	16683.0
17	19634.0
18	28091.0
19	48194.0
20	95763.0
21	112191.0
22	52046.0
23	32898.0
24	31189.0
25	32995.0
26	35143.0
27	38887.0
28	45868.0
29	53714.0
30	63058.0
31	77434.0
32	99635.0
33	140569.0
34	205256.0
35	418627.0
36	556419.0
37	912478.0
38	1054893.0
39	319261.0
40	1127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.160866989449069	42.279889144296426	25.1298418175052	19.42940204874931
2	14.594470517991907	41.8404646028451	25.007918972208532	18.557145906954464
3	18.104702868479013	26.355680463205474	19.006710821144857	36.53290584717066
4	32.196131332165585	27.808702224694443	21.518136034687064	18.47703040845291
5	16.90911453717446	40.36092627373269	21.830570192005002	20.899388997087847
6	36.18323770898186	24.34358111912495	23.580242547299935	15.892938624593253
7	18.575132563139018	23.73724674371213	39.72437975030119	17.963240942847666
8	35.280539379820816	24.153747893996712	23.128135683234543	17.437577042947932
9	34.70695585279086	25.92411402508156	23.106245831924994	16.26268429020259
10-11	16.325067112244824	43.68315080220122	23.126150627342746	16.86563145821121
12-13	16.183130192331305	25.694530921530202	31.887297855141682	26.235041030996808
14-15	16.422117904943807	34.3885767518064	23.065091175895045	26.124214167354747
16-17	25.695420400126523	25.26235279671596	32.031534402645704	17.010692400511818
18-19	16.691130943832245	25.438838366986722	32.20062211434703	25.669408574834
20-21	25.763270261346165	34.66802489325172	23.056738754929558	16.51196609047256
22-23	25.309625329920628	25.735262532861896	32.23017015942494	16.72494197779254
24-25	16.515860271156466	34.586800311187346	22.959980839329358	25.937358578326837
26-27	16.997692019237476	29.189074031870028	36.98409817850995	16.829135770382546
28-29	24.7062442699138	25.540911459564953	33.00404908012727	16.748795190393977
30-31	22.519786559848242	26.733062266538987	33.36712990422702	17.38002126938575
32-33	18.910813933660517	28.293435517791526	34.15123782878708	18.644512719760872
34-35	18.77002682754225	27.99919165920734	34.425620281153336	18.805161232097074
36-37	18.788445542866143	27.986641333159195	34.733238860582254	18.4916742633924
38-39	19.330409190526748	27.750235277936024	34.336477170121675	18.58287836141555
40-41	18.478982922444843	28.74234017913665	34.23831995282726	18.540356945591245
42-43	18.464600931878135	28.07929924083411	34.62465079519009	18.831449032097662
44-45	18.534065511617243	28.549171461534822	33.96137753767159	18.955385489176347
46-47	19.0726773435917	27.922078203825972	34.38312055992895	18.622123892653384
48-49	18.56321220659681	28.34396224098275	34.20518145146419	18.887644100956255
50-51	19.000195685291192	28.808606334562835	33.49391314610315	18.69728483404282
52-53	19.099204230105833	28.62584800242893	33.30161578353792	18.973331983927316
54-55	19.375056677142265	29.57130168901138	31.4650775516863	19.588564082160055
56-57	19.783121254156143	30.220762079227807	30.190064220354657	19.80605244626139
58-59	20.573726711389362	30.709801750407102	29.04667202667915	19.66979951152439
60-61	20.17489318663734	31.094501243317517	28.826818951187583	19.903786618857552
62-63	19.444702670530187	31.204318615241917	28.689534011774313	20.661444702453586
64-65	19.890064784414253	31.115371448431283	29.165341488188805	19.829222278965652
66-67	19.979622260093766	30.633353303214715	29.47929808849944	19.907726348192075
68-69	20.237319397879396	30.681978426022066	29.240177010579586	19.840525165518947
70-71	19.061678181437145	31.500418054940273	29.431404061489435	20.006499702133144
72-73	19.08256196057511	30.914628577155966	29.57819232472264	20.42461713754628
74-75	19.38587541217198	31.111746524456528	28.95718184249959	20.545196220871897
76-77	20.032522551116553	30.702863290520256	29.19087164385431	20.073742514508886
78-79	19.8555740514915	30.95950662783894	28.681975458522878	20.502943862146676
80-81	19.957254707856006	31.445776035973473	28.432930428094004	20.164038828076517
82-83	19.884323213784022	31.500609082102965	28.343166511006324	20.271901193106686
84-85	19.93955157565523	31.82263617003921	27.792867767146323	20.44494448715924
86-87	19.92932761000365	31.914074991354695	27.89223979148868	20.264357607152977
88-89	20.703926852751376	31.414648454194673	27.610945099211577	20.270479593842378
90-91	20.17931032388106	32.01679206515153	27.528827260757758	20.275070350209646
92-93	19.770831263306928	32.0761385000614	27.520402686485696	20.632627550145983
94-95	19.992066283091596	32.24614438063097	27.29636847504936	20.465420861228075
96-97	19.888811522502863	31.590394848071234	27.68776628442803	20.83302734499788
98-99	20.533059198644168	31.749498254983877	26.938224021149765	20.77921852522219
100	20.001943836151966	32.247026863555284	26.851753422648557	20.89927587764419
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156625.0
1	84534.0
2	9012.0
3	4755.0
4	3716.0
5	3382.5
6	3461.0
7	3921.0
8	3859.0
9	3386.0
10	3322.5
11	5116.0
12	36070.0
13	40455.5
14	13868.5
15	12665.0
16	14152.5
17	16331.5
18	18621.5
19	21117.5
20	24078.0
21	27451.5
22	31201.5
23	35796.5
24	41139.5
25	46495.5
26	53786.5
27	61817.0
28	67397.5
29	72310.5
30	78170.0
31	84463.0
32	91121.0
33	98572.5
34	107238.0
35	114353.5
36	119838.5
37	125360.0
38	129022.0
39	130700.0
40	131573.5
41	132001.0
42	130800.5
43	130137.5
44	132115.5
45	133248.0
46	133514.0
47	135589.5
48	136245.5
49	140381.0
50	161843.5
51	160596.5
52	131084.0
53	116511.5
54	111826.0
55	108396.5
56	99032.5
57	86287.5
58	78459.0
59	66340.5
60	51572.5
61	41895.0
62	33755.0
63	25871.5
64	19226.5
65	14468.5
66	11131.5
67	8683.0
68	6828.5
69	5325.0
70	4022.5
71	3060.0
72	2388.0
73	1653.5
74	1083.0
75	786.5
76	524.0
77	332.5
78	223.5
79	132.0
80	62.5
81	25.5
82	12.0
83	7.0
84	3.5
85	3.5
86	2.5
87	1.5
88	2.0
89	2.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13756545803157952
2	0.005597206776872342
3	0.0036880819847608455
4	0.0
5	0.002928770987898318
6	0.013212011345407969
7	0.002798603388436171
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.254189986553687E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.894785963246744E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.4101489941732644E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.254189986553687E-5
64-65	0.0
66-67	1.0847299955178957E-5
68-69	0.0
70-71	0.0
72-73	4.121973982968003E-4
74-75	4.121973982968003E-4
76-77	5.423649977589479E-4
78-79	4.5558659811751614E-4
80-81	9.87104295921285E-4
82-83	0.001019646195786822
84-85	0.001258286794800759
86-87	8.677839964143166E-5
88-89	0.0
90-91	6.508379973107374E-5
92-93	0.0
94-95	2.1694599910357914E-5
96-97	0.0013342178944870118
98-99	6.291433974003795E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4609442.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.372180164651446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31200769019074	55.331522818802
2	8.919101488892789	11.304458129218494
3	1.9148705265798176	3.640485600071916
4	0.6078796656228594	1.540906387531197
5	0.3034176811794152	0.9614119978421336
6	0.17376648070256462	0.6607176432996212
7	0.11726728348489418	0.5202038391496777
8	0.08818196601073514	0.4470626749844382
9	0.060663298054114724	0.3459928908300577
>10	0.4426137597029681	6.542652633165967
>50	0.04119668773671742	1.7393494239308742
>100	0.014959657657977278	1.7876947133571999
>500	0.0022936602685450507	0.9795574516647372
>1k	0.0014035828952880228	1.527000375089715
>5k	1.7116864576682331E-4	0.8032014162893867
>10k+	2.05402374920188E-4	11.867782004772634
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	329792	7.154705493636756	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145988	3.1671512517133307	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25986	0.5637558732705608	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	22501	0.48815019258296344	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11744	0.2547813813472433	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11026	0.23920465861160634	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9019	0.19566359659151802	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8429	0.18286378264440684	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7616	0.16522607291728586	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6074	0.13177299985551397	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5885	0.1276727204724563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001214897594980043	0.0	0.0	0.0	0.0
2	0.001214897594980043	0.0	0.0	0.0	0.0
3	0.001236592194890401	0.0	0.0	0.0	0.0
4	0.001236592194890401	2.1694599910357914E-5	0.0	0.0	0.0
5	0.001236592194890401	4.338919982071583E-5	0.0	0.0	0.0
6	0.001236592194890401	4.338919982071583E-5	0.0	0.0	0.0
7	0.001236592194890401	6.508379973107374E-5	0.0	0.0	0.0
8	0.001236592194890401	6.508379973107374E-5	0.0	0.0	0.0
9	0.001236592194890401	6.508379973107374E-5	0.0	0.0	0.0
10-11	0.001258286794800759	8.677839964143166E-5	0.0	0.0	0.0
12-13	0.0012691340947559378	9.762569959661061E-5	0.0	0.0	0.0
14-15	0.0013450651944421905	1.0847299955178956E-4	0.0	0.0	0.0
16-17	0.0014426908940388013	1.0847299955178956E-4	0.0	0.0	0.0
18-19	0.0014643854939491593	1.5186219937250538E-4	0.0	0.0	0.0
20-21	0.001496927393814696	1.5186219937250538E-4	0.0	0.0	0.0
22-23	0.0015620111935457698	1.9525139919322122E-4	0.0	0.0	0.0
24-25	0.0016270949932768435	1.9525139919322122E-4	0.0	0.0	0.0
26-27	0.0016704841930975593	1.9525139919322122E-4	0.0	0.0	0.0
28-29	0.0017898044926045278	1.9525139919322122E-4	0.0	0.0	0.0
30-31	0.0018548882923356018	2.060986991484002E-4	0.0	0.0	0.0
32-33	0.002039292391573644	2.49487898969116E-4	0.0	0.0	0.0
34-35	0.0024840316897359813	2.6033519892429494E-4	0.0	0.0	0.0
36-37	0.003026396687494929	2.6033519892429494E-4	0.0	0.0	0.0
38-39	0.0041436685828783616	2.820297988346529E-4	0.0	0.0	0.0
40-41	0.006681936772390237	3.0372439874501076E-4	0.0	0.0	0.0
42-43	0.00965409696010927	3.0372439874501076E-4	0.0	0.0	0.0
44-45	0.012658799047693844	3.0372439874501076E-4	0.0	0.0	0.0
46-47	0.01568519573518877	3.796554984312635E-4	0.0	0.0	0.0
48-49	0.01914548442089086	3.9050279838644244E-4	0.0	0.0	0.0
50-51	0.023723045001976376	3.9050279838644244E-4	0.0	0.0	0.0
52-53	0.02996024247620428	4.3389199820715826E-4	0.0	0.0	0.0
54-55	0.038095717442588495	4.555865981175162E-4	0.0	0.0	0.0
56-57	0.046404749208255575	5.09823097893411E-4	0.0	0.0	0.0
58-59	0.05573342716970948	5.423649977589479E-4	0.0	0.0	0.0
60-61	0.06969390221202479	5.423649977589479E-4	0.0	0.0	0.0
62-63	0.08704958214031114	5.640595976693058E-4	0.0	0.0	0.0
64-65	0.10606489896173985	5.966014975348425E-4	0.0	2.1694599910357914E-5	0.0
66-67	0.1299506534630439	6.074487974900215E-4	0.0	2.1694599910357914E-5	0.0
68-69	0.15999767433888962	6.291433974003795E-4	0.0	2.1694599910357914E-5	0.0
70-71	0.1983537269804024	6.399906973555585E-4	0.0	2.1694599910357914E-5	0.0
72-73	0.24057141840595891	6.616852972659163E-4	0.0	2.1694599910357914E-5	0.0
74-75	0.28736667041260094	7.2676909699699E-4	0.0	2.1694599910357914E-5	0.0
76-77	0.34234078658544786	7.37616396952169E-4	0.0	2.1694599910357914E-5	0.0
78-79	0.4052442790255306	7.59310996862527E-4	0.0	2.1694599910357914E-5	0.0
80-81	0.4824445128065393	7.810055967728849E-4	0.0	2.1694599910357914E-5	0.0
82-83	0.5796797096047634	7.810055967728849E-4	0.0	2.1694599910357914E-5	0.0
84-85	0.6956807353254473	7.810055967728849E-4	0.0	2.1694599910357914E-5	0.0
86-87	0.8300028506704282	7.918528967280638E-4	0.0	2.1694599910357914E-5	0.0
88	0.9429340905037963	8.243947965936006E-4	0.0	2.1694599910357914E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	6170	0.0	88.20511	1
ATCGAGT	2190	0.0	77.17407	1
AACGAGT	89355	0.0	74.404366	1
GTGGAAT	106160	0.0	74.18944	6
ACGAGTG	97745	0.0	73.714294	2
TGGAATC	106680	0.0	73.69524	7
CGAGTGG	101210	0.0	73.43102	3
GGAATCA	107330	0.0	73.29751	8
GAATCAC	107670	0.0	73.11842	9
TCGAGTG	2400	0.0	73.06595	2
AACGTGT	3320	0.0	72.74453	1
AGTGGAA	105515	0.0	72.02851	5
GAGTGGA	105745	0.0	71.991875	4
CGTGTGG	3765	0.0	71.296745	3
ACGTGTG	3745	0.0	68.29117	2
GCGAGTG	1215	0.0	54.94517	2
GTGTGGA	5900	0.0	47.806664	4
TGAGTGG	4725	0.0	44.075832	3
ATGAGTG	4325	0.0	43.80628	2
AGCGAGT	1475	0.0	43.088642	1
>>END_MODULE