FastQCFastQC Report
Thu 26 May 2016
SRR2132766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124744
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1435746.757237577797608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA784773.6934802498559827No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117840.5546079904214343No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG100530.4731393523172674No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63750.30003614553094393No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49430.23263979095834603No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA44100.2075544159672883No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41720.19635306653413304No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38480.1811041706671486No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33870.15940743920208741No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC29990.14114641575643938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT27200.086.360391
AACGAGT388750.073.96251
GTGGAAT461500.073.798026
TGGAATC465000.073.156527
GAATCAC466400.073.1485449
ACGAGTG426800.073.0668262
GGAATCA467400.072.961878
CGAGTGG442300.072.807413
GAGTGGA461000.071.748854
AGTGGAA461500.071.6813055
ATCGAGT10450.071.1743851
AACGTGT12750.069.780581
ACGTGTG13700.069.653782
CGTGTGG15050.067.773793
TCGAGTG11550.067.1538852
TGAGTGG19100.050.6958473
GCGAGTG6250.050.3922772
ATGAGTG18200.048.8157122
AGCGAGT6100.048.617621
AATGAGT19200.043.3965261