##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132766_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2124744
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.86298584676554	31.0	25.0	33.0	16.0	34.0
2	28.144391041932582	31.0	25.0	34.0	16.0	34.0
3	29.21007942603909	31.0	28.0	34.0	16.0	34.0
4	34.72535656060212	35.0	35.0	37.0	32.0	37.0
5	33.82397926526678	35.0	35.0	37.0	28.0	37.0
6	35.23348695183984	37.0	35.0	37.0	32.0	37.0
7	35.63273034304368	37.0	35.0	37.0	33.0	37.0
8	35.90224139943447	37.0	35.0	37.0	35.0	37.0
9	37.86306585640435	39.0	38.0	39.0	35.0	39.0
10-11	37.85982546603262	39.0	38.0	39.0	35.0	39.0
12-13	37.83125472998159	39.0	38.0	39.0	35.0	39.0
14-15	39.239378249803266	41.0	39.0	41.0	36.0	41.0
16-17	39.18245751017534	40.5	39.0	41.0	36.0	41.0
18-19	38.96566927592218	40.0	38.5	41.0	35.5	41.0
20-21	38.85544658556513	40.0	39.0	41.0	35.0	41.0
22-23	38.699325424615864	40.0	39.0	41.0	35.0	41.0
24-25	38.53488655574507	40.0	38.0	41.0	35.0	41.0
26-27	38.32098055106874	40.0	38.0	41.0	34.5	41.0
28-29	38.15152484252221	40.0	38.0	41.0	34.0	41.0
30-31	38.02816598140764	40.0	38.0	41.0	34.0	41.0
32-33	37.87841335238504	40.0	38.0	41.0	34.0	41.0
34-35	37.789347563753566	40.0	38.0	41.0	33.0	41.0
36-37	37.63510945318589	40.0	37.5	41.0	33.0	41.0
38-39	37.1823005500898	40.0	36.0	41.0	33.0	41.0
40-41	36.934694014902504	40.0	35.0	41.0	33.0	41.0
42-43	36.78163157538037	40.0	35.0	41.0	32.5	41.0
44-45	36.643411629824584	40.0	35.0	41.0	32.0	41.0
46-47	36.517591295704335	39.0	35.0	41.0	31.5	41.0
48-49	36.365020915460875	39.0	35.0	41.0	31.0	41.0
50-51	36.104969586924355	39.0	35.0	41.0	30.5	41.0
52-53	35.69886560451518	39.0	35.0	41.0	29.5	41.0
54-55	34.91763243007158	39.0	35.0	41.0	24.5	41.0
56-57	34.26642644949227	39.0	35.0	41.0	19.0	41.0
58-59	33.94302301830244	38.0	35.0	40.5	14.0	41.0
60-61	33.701196002906705	38.0	34.0	40.0	9.0	41.0
62-63	33.435232903352116	37.0	34.0	40.0	7.5	41.0
64-65	33.163974106998296	37.0	34.0	40.0	6.0	41.0
66-67	32.863616275654856	36.0	34.0	40.0	2.0	41.0
68-69	32.55294073074215	36.0	34.0	39.0	2.0	41.0
70-71	32.23621480987828	35.0	33.0	39.0	2.0	41.0
72-73	31.92449090337471	35.0	33.0	38.5	2.0	40.0
74-75	31.597092167338747	35.0	33.0	37.5	2.0	40.0
76-77	31.27545130142737	35.0	33.0	37.0	2.0	39.0
78-79	30.948777829234956	35.0	33.0	36.5	2.0	39.0
80-81	30.645942993602993	35.0	33.0	36.0	2.0	39.0
82-83	30.355251267917453	35.0	32.5	36.0	2.0	37.0
84-85	30.076867142582824	35.0	32.0	35.0	2.0	37.0
86-87	29.874749381572556	35.0	32.0	35.0	2.0	36.5
88-89	29.660282368134702	35.0	32.0	35.0	2.0	36.0
90-91	29.47059951693004	35.0	32.0	35.0	2.0	36.0
92-93	29.302709173434543	35.0	32.0	35.0	2.0	36.0
94-95	29.12116283185174	35.0	31.5	35.0	2.0	35.5
96-97	28.939053598927682	35.0	31.0	35.0	2.0	35.0
98-99	28.779840771405873	35.0	31.0	35.0	2.0	35.0
100	28.67453632061086	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.254331121751882
1101	2	2.2600065259903452
1101	3	1.0228727387680898
1101	4	0.5778482222329515
1101	5	2.214057475917322
1101	6	1.019837260508929
1101	7	0.534943987123043
1101	8	0.3950606411818285
1101	9	0.3615745957742291
1101	10-11	0.4935082356359004
1101	12-13	0.4357633314752647
1101	14-15	0.5381241729475974
1101	16-17	0.5058817226192716
1101	18-19	0.4067286968329
1101	20-21	0.3558982463466265
1101	22-23	0.3037515602501202
1101	24-25	0.2547240225112333
1101	26-27	0.2038992308522296
1101	28-29	0.201119798367138
1101	30-31	0.19798973491865013
1101	32-33	0.3082304363996471
1101	34-35	0.2789547889676527
1101	36-37	0.36287348674655817
1101	38-39	0.26946923134188694
1101	40-41	0.3222469223094535
1101	42-43	0.3919863898235434
1101	44-45	0.43819335572217
1101	46-47	0.40658563009505144
1101	48-49	0.3723361803039893
1101	50-51	0.4791051483477915
1101	52-53	0.5561241350818804
1101	54-55	0.6882651814479388
1101	56-57	0.6382690126610768
1101	58-59	0.5826605352612546
1101	60-61	0.5414649829557519
1101	62-63	0.5882376294715073
1101	64-65	0.6450962459834102
1101	66-67	0.5166464654017986
1101	68-69	0.557479870244677
1101	70-71	0.6161067863199818
1101	72-73	0.6565724520600433
1101	74-75	0.6649209325291352
1101	76-77	0.5625856271124015
1101	78-79	0.6987123064146701
1101	80-81	0.6564843220743555
1101	82-83	0.6391196158203449
1101	84-85	0.6615504900950526
1101	86-87	0.65446127290992
1101	88-89	0.5479778396761255
1101	90-91	0.4371561218275417
1101	92-93	0.5099873248878453
1101	94-95	0.5813247801677264
1101	96-97	0.48684167597625816
1101	98-99	0.4748508544552337
1101	100	0.5159257640347334
1102	1	1.472982366853639
1102	2	3.112344096422028
1102	3	3.2460499752881518
1102	4	1.4526010083963428
1102	5	2.646218615046827
1102	6	1.170563797962508
1102	7	0.6915633459944246
1102	8	0.5165019812077247
1102	9	0.3837014160991643
1102	10-11	0.4965947383327389
1102	12-13	0.47275358823923597
1102	14-15	0.5910611529525127
1102	16-17	0.5111707550340228
1102	18-19	0.39686166727075545
1102	20-21	0.3213731606740424
1102	22-23	0.24900097375488883
1102	24-25	0.28988536966365785
1102	26-27	0.23509881142229005
1102	28-29	0.25445018056196744
1102	30-31	0.30527904701972375
1102	32-33	0.3044152314174511
1102	34-35	0.33565745653807966
1102	36-37	0.3888277047844326
1102	38-39	0.3263137327189227
1102	40-41	0.34323036308811794
1102	42-43	0.42396083087393066
1102	44-45	0.4039310358386601
1102	46-47	0.42698792551347253
1102	48-49	0.4169091958499678
1102	50-51	0.4677468731663268
1102	52-53	0.46706370193636104
1102	54-55	0.5375461089315507
1102	56-57	0.46718795538709657
1102	58-59	0.46917159282388354
1102	60-61	0.38559585027196164
1102	62-63	0.3647649058543365
1102	64-65	0.41107743235104266
1102	66-67	0.41399586527061416
1102	68-69	0.39182631997032047
1102	70-71	0.39331113250594285
1102	72-73	0.3413009075048201
1102	74-75	0.41095443815321886
1102	76-77	0.4421357551609546
1102	78-79	0.4368614697942732
1102	80-81	0.4359679624156705
1102	82-83	0.4764571414624079
1102	84-85	0.4292268392829186
1102	86-87	0.45255948062827045
1102	88-89	0.5023336056433791
1102	90-91	0.520839063052609
1102	92-93	0.4820592530413066
1102	94-95	0.4704796696518194
1102	96-97	0.48238958743613125
1102	98-99	0.527409362144617
1102	100	0.5299225212660197
1103	1	1.1878823200089599
1103	2	2.9307391107450655
1103	3	3.1965057200632856
1103	4	1.3390094900765703
1103	5	2.558372826941998
1103	6	1.2069804985769466
1103	7	0.7132236486721055
1103	8	0.5343213127907234
1103	9	0.4225586204010767
1103	10-11	0.4507386080148734
1103	12-13	0.460254148621118
1103	14-15	0.5509468253035692
1103	16-17	0.4711397537339437
1103	18-19	0.39763848649773337
1103	20-21	0.3168483612582591
1103	22-23	0.29911308261645786
1103	24-25	0.3800261245971299
1103	26-27	0.43407782812589346
1103	28-29	0.47622926795489917
1103	30-31	0.49108738407268504
1103	32-33	0.5261527368507117
1103	34-35	0.5480498226100607
1103	36-37	0.5195413259299002
1103	38-39	0.5918848771778968
1103	40-41	0.6415300832545228
1103	42-43	0.7149342483067755
1103	44-45	0.7892356464333545
1103	46-47	0.798603381397939
1103	48-49	0.7913134043341188
1103	50-51	0.8173279511656375
1103	52-53	0.8779288730818635
1103	54-55	0.9603617078839477
1103	56-57	0.91648923227811
1103	58-59	0.8237149741778751
1103	60-61	0.7862229257152862
1103	62-63	0.7350115680899236
1103	64-65	0.7657296414901111
1103	66-67	0.7414929431633723
1103	68-69	0.6743032111210994
1103	70-71	0.6894900915145783
1103	72-73	0.7522563082542071
1103	74-75	0.7656119262520065
1103	76-77	0.7037508472032741
1103	78-79	0.7369721167900778
1103	80-81	0.6287678349409767
1103	82-83	0.6757956959305496
1103	84-85	0.7138406533296902
1103	86-87	0.7053444161340003
1103	88-89	0.6753864329242667
1103	90-91	0.6528218273303281
1103	92-93	0.6633159898060619
1103	94-95	0.6636770884516743
1103	96-97	0.6750487147737374
1103	98-99	0.6316827044050797
1103	100	0.7287385399363195
1104	1	6.103749883579404
1104	2	5.136316999346629
1104	3	4.158810734353189
1104	4	1.8698168964649753
1104	5	2.8651847224810894
1104	6	1.281612628612244
1104	7	0.7266046402469968
1104	8	0.5879729626647574
1104	9	0.47798826790949533
1104	10-11	0.46910921738666644
1104	12-13	0.4340359014186177
1104	14-15	0.4956185372469477
1104	16-17	0.37702267793173405
1104	18-19	0.3404402979679162
1104	20-21	0.35620477737332124
1104	22-23	0.37232297665761394
1104	24-25	0.4009840246824936
1104	26-27	0.4755038143425594
1104	28-29	0.49773646216006995
1104	30-31	0.5603722047498252
1104	32-33	0.5773359405473926
1104	34-35	0.5622139375351622
1104	36-37	0.5305278644992129
1104	38-39	0.5910181496492442
1104	40-41	0.5189216992762056
1104	42-43	0.6279354545846161
1104	44-45	0.6447079915143448
1104	46-47	0.573905803881118
1104	48-49	0.6928345912386362
1104	50-51	0.7460532275264669
1104	52-53	0.6819476711301391
1104	54-55	0.6248739265226888
1104	56-57	0.6683154393394943
1104	58-59	0.6115691783718589
1104	60-61	0.5647123811672259
1104	62-63	0.6811825198760246
1104	64-65	0.6856392316381843
1104	66-67	0.7050640628371596
1104	68-69	0.624344079128548
1104	70-71	0.5877164786607452
1104	72-73	0.6224996126248321
1104	74-75	0.6158568100699924
1104	76-77	0.539204121576752
1104	78-79	0.6374135514840944
1104	80-81	0.6183361100655773
1104	82-83	0.5899086139109144
1104	84-85	0.6175824459545822
1104	86-87	0.6719282197118872
1104	88-89	0.6857582144528784
1104	90-91	0.665042781980425
1104	92-93	0.5890808445239237
1104	94-95	0.6344302161974689
1104	96-97	0.5852902883911923
1104	98-99	0.6840976608915348
1104	100	0.6966002600147085
1105	1	1.7292651314854446
1105	2	3.123888205312724
1105	3	3.2454992000855185
1105	4	1.4209952532230474
1105	5	2.629879372994587
1105	6	1.2007424150891808
1105	7	0.6411948111894858
1105	8	0.5047168487809728
1105	9	0.3971455252432392
1105	10-11	0.4240864022756128
1105	12-13	0.39849632712808614
1105	14-15	0.4663894327095761
1105	16-17	0.37877994724728126
1105	18-19	0.3204070062999733
1105	20-21	0.32897506200451687
1105	22-23	0.22505017042756492
1105	24-25	0.2572703878180178
1105	26-27	0.3259224024639451
1105	28-29	0.3925159682686896
1105	30-31	0.4022154826838573
1105	32-33	0.4366559141633317
1105	34-35	0.44783823495858144
1105	36-37	0.4405559163834667
1105	38-39	0.43086568865183494
1105	40-41	0.5410098110333692
1105	42-43	0.5245184788896182
1105	44-45	0.6107479162276377
1105	46-47	0.6572775211513573
1105	48-49	0.5649678003585379
1105	50-51	0.48521221138474147
1105	52-53	0.5067831771448326
1105	54-55	0.48486520509012365
1105	56-57	0.5245091165567786
1105	58-59	0.5709169389476187
1105	60-61	0.5790873913276826
1105	62-63	0.530313552701962
1105	64-65	0.5477821180259497
1105	66-67	0.529749081240368
1105	68-69	0.47411653764529404
1105	70-71	0.5323975487868395
1105	72-73	0.533541564913147
1105	74-75	0.43973268003063026
1105	76-77	0.41226023859145755
1105	78-79	0.456944811362316
1105	80-81	0.4539231073338428
1105	82-83	0.38278956771110373
1105	84-85	0.3713892669758536
1105	86-87	0.4829401647930567
1105	88-89	0.45297081359633395
1105	90-91	0.42655261940713984
1105	92-93	0.46474213954677523
1105	94-95	0.4946627867290836
1105	96-97	0.4969534583564652
1105	98-99	0.49263847069325806
1105	100	0.5265165198116435
1106	1	-0.7085603805552765
1106	2	1.8408020012527402
1106	3	2.678528435701601
1106	4	1.1960905280344036
1106	5	2.432075175009601
1106	6	1.1197781627877248
1106	7	0.6262835665517628
1106	8	0.43898643720851993
1106	9	0.36726717251526253
1106	10-11	0.42760503243009396
1106	12-13	0.39769413408854604
1106	14-15	0.4079969311810032
1106	16-17	0.37569901645166937
1106	18-19	0.3078971227660645
1106	20-21	0.32985353014269947
1106	22-23	0.2674847549385291
1106	24-25	0.27586017941664664
1106	26-27	0.24706239136492059
1106	28-29	0.3327680106610629
1106	30-31	0.346457004330631
1106	32-33	0.27073332617532486
1106	34-35	0.36421739924134044
1106	36-37	0.34553310445053853
1106	38-39	0.38027151023747763
1106	40-41	0.4025840124315465
1106	42-43	0.4026236581069398
1106	44-45	0.47747183781745406
1106	46-47	0.42004784501204995
1106	48-49	0.5232189243803163
1106	50-51	0.5307668618023058
1106	52-53	0.5955646294963728
1106	54-55	0.6188300614535223
1106	56-57	0.5541161325153041
1106	58-59	0.6534639379138341
1106	60-61	0.6518670339489105
1106	62-63	0.6201864909722374
1106	64-65	0.620301166849373
1106	66-67	0.6424528293710949
1106	68-69	0.5828757735314838
1106	70-71	0.5865995240203077
1106	72-73	0.5873520355657966
1106	74-75	0.5623920934520967
1106	76-77	0.6265601160601975
1106	78-79	0.6589122178002427
1106	80-81	0.6990657494418713
1106	82-83	0.8064822401450087
1106	84-85	0.7235375850562171
1106	86-87	0.7427957669765171
1106	88-89	0.7854791378646446
1106	90-91	0.8467406275496998
1106	92-93	0.8855629087189882
1106	94-95	0.9233134940463366
1106	96-97	0.9865135919867569
1106	98-99	1.0171221634761878
1106	100	0.9546662977083784
1107	1	-0.8309938871903348
1107	2	1.8545765199298643
1107	3	-0.4637182883924389
1107	4	0.012888808504420979
1107	5	1.9092739467001465
1107	6	0.8243661573760406
1107	7	0.41069005734831876
1107	8	0.33541010071858324
1107	9	0.306371992148847
1107	10-11	0.3507644638868541
1107	12-13	0.3303347848460447
1107	14-15	0.4059351617481255
1107	16-17	0.3475316378314375
1107	18-19	0.25942535455607185
1107	20-21	0.24096137699056897
1107	22-23	0.2087247899279916
1107	24-25	0.06132479969679139
1107	26-27	0.0013542387626017671
1107	28-29	-0.003945529104917966
1107	30-31	0.03214636671515336
1107	32-33	0.06775085704358474
1107	34-35	0.0712966666309569
1107	36-37	0.11323405588378677
1107	38-39	0.12926294969367547
1107	40-41	1.8196656668578726E-5
1107	42-43	0.053040263064517035
1107	44-45	0.16259230687756343
1107	46-47	0.15911166238023355
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2216	82-83	0.2359789210991856
2216	84-85	0.20621364541136344
2216	86-87	0.08749029235578831
2216	88-89	0.15840349012112753
2216	90-91	0.13484934334650234
2216	92-93	-1.2581851649429154E-7
2216	94-95	-0.07534135133274233
2216	96-97	-0.13289492323824703
2216	98-99	-0.19329977856150293
2216	100	-0.20340832890453697
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1040.0
3	284.0
4	345.0
5	1052.0
6	3015.0
7	5427.0
8	6470.0
9	8959.0
10	6105.0
11	4829.0
12	4184.0
13	5687.0
14	5721.0
15	6289.0
16	7690.0
17	8727.0
18	12173.0
19	21008.0
20	41015.0
21	48009.0
22	22488.0
23	14379.0
24	13969.0
25	14830.0
26	15803.0
27	17686.0
28	21081.0
29	24904.0
30	29328.0
31	36001.0
32	46443.0
33	66127.0
34	97351.0
35	204551.0
36	260251.0
37	429744.0
38	478179.0
39	132971.0
40	629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.359236259572205	41.988662285414804	25.161469114436642	19.49063234057635
2	14.74366728308453	41.491386074165	25.167922486669514	18.59702415608096
3	18.07283069787888	26.70339688640318	19.370296058561262	35.85347635715667
4	31.555425030027145	28.200715003783984	21.59624877161672	18.647611194572146
5	17.052926754996374	40.13811890764351	22.035062980922874	20.77389135643724
6	35.48269725096247	24.836796048367397	23.634530468267897	16.04597623240224
7	18.613097804293517	24.30822914568323	39.07479295822642	18.003880091796834
8	34.522088308050286	24.701940563192554	23.259790355920526	17.516180772836634
9	33.95877338634678	26.347126995063874	23.22548034021981	16.468619278369534
10-11	16.483656384016147	43.28253191913943	23.238211285689005	16.995600411155415
12-13	16.32363710639964	26.171623499113306	31.550012613284235	25.95472678120282
14-15	16.572725937807096	34.41169853874161	23.195194338706216	25.820381184745077
16-17	25.41496763845433	25.753361346119814	31.656519561886043	17.175151453539815
18-19	16.882339707748322	25.911050931312197	31.8577438034888	25.348865557450683
20-21	25.52060389392793	34.659304838606445	23.14307041224731	16.677020855218323
22-23	25.043534656410372	26.180965801056505	31.860402947366833	16.915096595166286
24-25	16.70474184184071	34.60814573426257	23.01157221764128	25.675540206255437
26-27	17.233539013062046	29.489536031055042	36.192956938096295	17.083968017786617
28-29	24.568277401889357	25.984518605535538	32.450827017278314	16.99637697529679
30-31	22.506076026100086	27.098723422680564	32.765264897794744	17.6299356534246
32-33	19.097241832427812	28.565935472696946	33.54037003987304	18.796452655002202
34-35	18.983322226112886	28.197749940698742	33.790659015862616	19.02826881732576
36-37	18.90408915144601	28.356216090032493	34.13213544784689	18.607559310674603
38-39	19.510350423392183	27.93661259897663	33.785952566520955	18.767084411110233
40-41	18.676179342076033	29.09962329579469	33.60703689479768	18.617160467331594
42-43	18.740870866666118	28.30663769033368	33.982508304025586	18.96998313897462
44-45	18.75272974061816	28.81257695044674	33.40044730094543	19.03424600798967
46-47	19.188899933356677	28.241731709796568	33.694059143125	18.875309213721746
48-49	18.799394185840743	28.570924308999107	33.63694638036394	18.992735124796212
50-51	19.130963541960817	29.06472497392627	32.927449142108415	18.876862342004497
52-53	19.285984066304504	28.832482053707686	32.75599278500806	19.125541094979745
54-55	19.57845274536603	29.675151453539815	31.035009394072887	19.71138640702127
56-57	19.820340709280742	30.31464025783812	29.866209764564577	19.99880926831656
58-59	20.579420391350673	30.714853177606336	28.933132650333405	19.77259378070958
60-61	20.300751525830876	30.956929399494715	28.61316469184052	20.129154382833885
62-63	19.533049298915046	31.129230953868525	28.551624490967733	20.7860952562487
64-65	20.01549363123275	31.07619082581243	28.94071003377348	19.96760550918134
66-67	20.090867417439465	30.424229930758717	29.31934388331018	20.16555876849164
68-69	20.32670759395014	30.647833338981073	29.12212012364784	19.90333894342095
70-71	19.13526994310844	31.487228579066468	29.237545793752094	20.139955684072998
72-73	19.253397429275182	30.865876464861557	29.370110999907517	20.51061510595574
74-75	19.571400265350988	30.969467693117203	28.880875855931077	20.578256185600733
76-77	20.09040675982201	30.59792759599394	29.142425357004097	20.169240287179953
78-79	19.954789613565563	30.87505583054436	28.626655678619382	20.543498877270697
80-81	20.065961632591588	31.259271809932603	28.442077073685002	20.232689483790807
82-83	19.982877675392636	31.337666313756007	28.305842934440744	20.37361307641061
84-85	19.999990586955118	31.566669537645353	27.879179803747427	20.5541600716521
86-87	19.964762805486874	31.80760407079725	27.872611446981715	20.355021676734157
88-89	20.81027173155919	31.186910046575022	27.66385032738062	20.338967894485172
90-91	20.357478612114175	31.824138689747112	27.538890006671394	20.27949269146732
92-93	19.840860828410385	31.941542134017087	27.516750253206972	20.700846784365552
94-95	20.082264066760075	32.08865731055474	27.312173754661153	20.51690486802404
96-97	19.928710472364305	31.423446710054424	27.74766133450306	20.90018148307821
98-99	20.47819342352849	31.532512898133692	26.96931274903211	21.01998092930571
100	20.05352174191338	32.119445919131905	26.851846622463693	20.975185716491023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85374.0
1	46146.5
2	4924.5
3	2438.0
4	1845.5
5	1646.5
6	1670.0
7	1917.0
8	1888.0
9	1625.0
10	1538.5
11	2460.5
12	17978.0
13	19854.0
14	6194.5
15	5577.5
16	6201.5
17	7001.5
18	7908.5
19	8893.5
20	9993.5
21	11263.0
22	12946.0
23	14987.5
24	16990.5
25	19296.0
26	23030.5
27	26533.5
28	28627.0
29	30359.0
30	32491.0
31	35299.0
32	38095.0
33	41512.0
34	45370.0
35	48528.0
36	51440.0
37	54171.5
38	56368.5
39	57397.5
40	58177.0
41	59243.0
42	59985.0
43	60741.0
44	61963.0
45	63223.0
46	64125.0
47	66398.5
48	67650.0
49	68628.0
50	78046.0
51	77846.0
52	64212.0
53	56944.0
54	53939.5
55	52260.0
56	48138.0
57	41864.5
58	38317.0
59	32261.5
60	24512.0
61	19673.0
62	15383.5
63	11662.5
64	8755.5
65	6580.0
66	4969.0
67	3808.0
68	2970.0
69	2187.0
70	1521.0
71	1087.0
72	826.0
73	588.0
74	385.0
75	287.0
76	200.0
77	116.5
78	75.5
79	44.5
80	19.0
81	11.5
82	6.5
83	4.0
84	2.5
85	2.5
86	4.0
87	3.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14006393240785714
2	0.004847642821911723
3	0.0029650630852469757
4	0.0
5	0.002494418151080789
6	0.012001445821237758
7	0.0024473536576641703
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.353224670830933E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0010118866084573013
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	7.059674012492799E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	7.059674012492799E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	5.883061677077333E-4
74-75	5.177094275828053E-4
76-77	6.35370661124352E-4
78-79	5.177094275828053E-4
80-81	0.0011295478419988479
82-83	0.0015295960360401065
84-85	0.0015531282827484158
86-87	2.353224670830933E-5
88-89	0.0
90-91	7.059674012492799E-5
92-93	0.0
94-95	4.706449341661866E-5
96-97	0.0013178058156653224
98-99	8.471608814991359E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2124744.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.3345135160323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.56852014218428	62.57290277299168
2	5.964683508161417	8.151874916146227
3	1.041542468940292	2.1351989376396605
4	0.3949403926291538	1.0795223839257606
5	0.192768184415201	0.6586360051690779
6	0.13186744340458134	0.5406658556192994
7	0.07608043258991362	0.3639243544384726
8	0.057776343603627295	0.3158494666311197
9	0.045964594271179174	0.2826871369634551
>10	0.4849202055422204	6.50953804991876
>50	0.024111924158177842	1.096080063680304
>100	0.013860208471186202	2.0042642215374995
>500	0.001585476452617159	0.7158630240611624
>1k	0.0010340063821416235	1.7842168692745026
>5k	6.893375880944158E-5	0.30029787315851186
>10k+	2.757350352377663E-4	11.488478068844415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	143574	6.757237577797608	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78477	3.6934802498559827	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11784	0.5546079904214343	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	10053	0.4731393523172674	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6375	0.30003614553094393	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4943	0.23263979095834603	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	4410	0.2075544159672883	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4172	0.19635306653413304	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3848	0.1811041706671486	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3387	0.15940743920208741	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	2999	0.14114641575643938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.294514539163306E-4	4.706449341661866E-5	0.0	0.0	0.0
2	3.765159473329493E-4	4.706449341661866E-5	0.0	0.0	0.0
3	4.235804407495679E-4	4.706449341661866E-5	0.0	0.0	0.0
4	4.235804407495679E-4	4.706449341661866E-5	0.0	0.0	0.0
5	4.235804407495679E-4	1.4119348024985598E-4	0.0	0.0	0.0
6	4.235804407495679E-4	1.4119348024985598E-4	0.0	0.0	0.0
7	4.235804407495679E-4	1.4119348024985598E-4	0.0	0.0	0.0
8	4.235804407495679E-4	1.8825797366647465E-4	0.0	0.0	0.0
9	4.706449341661866E-4	1.8825797366647465E-4	0.0	0.0	0.0
10-11	4.706449341661866E-4	3.059192072080213E-4	0.0	0.0	0.0
12-13	4.94177180874496E-4	3.294514539163306E-4	0.0	0.0	0.0
14-15	5.647739209994239E-4	3.294514539163306E-4	0.0	0.0	0.0
16-17	6.824351545409706E-4	3.765159473329493E-4	0.0	0.0	0.0
18-19	7.765641413742079E-4	4.706449341661866E-4	0.0	0.0	0.0
20-21	8.000963880825172E-4	5.647739209994239E-4	0.0	0.0	0.0
22-23	8.706931282074452E-4	6.118384144160426E-4	0.0	0.0	0.0
24-25	9.412898683323732E-4	6.118384144160426E-4	0.0	0.0	0.0
26-27	9.88354361748992E-4	6.118384144160426E-4	0.0	0.0	0.0
28-29	0.0011295478419988479	6.118384144160426E-4	0.0	0.0	4.706449341661866E-5
30-31	0.0012236768288320851	6.118384144160426E-4	0.0	0.0	7.059674012492799E-5
32-33	0.001294273568957013	6.589029078326612E-4	0.0	0.0	9.412898683323732E-5
34-35	0.0015060637893317972	7.0596740124928E-4	0.0	0.0	9.412898683323732E-5
36-37	0.0018825797366647467	7.530318946658986E-4	0.0	0.0	9.412898683323732E-5
38-39	0.0027062083714555728	7.530318946658986E-4	0.0	0.0	9.412898683323732E-5
40-41	0.004753513835078485	7.530318946658986E-4	0.0	0.0	9.412898683323732E-5
42-43	0.007271464232867583	7.765641413742079E-4	0.0	0.0	9.412898683323732E-5
44-45	0.009389366436615422	8.471608814991359E-4	0.0	0.0	9.412898683323732E-5
46-47	0.011483736393654954	8.471608814991359E-4	0.0	0.0	9.412898683323732E-5
48-49	0.014213477011818836	9.648221150406827E-4	0.0	0.0	9.412898683323732E-5
50-51	0.017790378511481852	0.0010354188551656106	0.0	0.0	9.412898683323732E-5
52-53	0.02212031190581077	0.0010354188551656106	0.0	0.0	9.412898683323732E-5
54-55	0.02819163155655458	0.0011766123354154665	0.0	0.0	9.412898683323732E-5
56-57	0.035063047595380904	0.0012236768288320851	0.0	0.0	9.412898683323732E-5
58-59	0.04304047922949777	0.0012707413222487038	0.0	0.0	9.412898683323732E-5
60-61	0.05339466778115387	0.0012707413222487038	0.0	0.0	9.412898683323732E-5
62-63	0.067325757832473	0.0013178058156653224	0.0	0.0	9.412898683323732E-5
64-65	0.08193928303833309	0.0013648703090819413	0.0	4.706449341661866E-5	1.4119348024985598E-4
66-67	0.09907075864198228	0.0014354670492068692	0.0	4.706449341661866E-5	1.6472572695816533E-4
68-69	0.12168524772866754	0.0014589992959151785	0.0	4.706449341661866E-5	1.8825797366647465E-4
70-71	0.15270074889021923	0.0014825315426234878	0.0	4.706449341661866E-5	2.1179022037478396E-4
72-73	0.1858812167489354	0.0016001927761650344	0.0	4.706449341661866E-5	2.353224670830933E-4
74-75	0.22480355280447903	0.0016943217629982717	0.0	4.706449341661866E-5	2.353224670830933E-4
76-77	0.27029138569164096	0.0016943217629982717	0.0	4.706449341661866E-5	2.353224670830933E-4
78-79	0.3199914907395903	0.0017413862564148906	0.0	4.706449341661866E-5	2.353224670830933E-4
80-81	0.38651715218398075	0.0017413862564148906	0.0	4.706449341661866E-5	2.353224670830933E-4
82-83	0.4668562424461488	0.0017413862564148906	0.0	4.706449341661866E-5	2.353224670830933E-4
84-85	0.5663505815288807	0.0018825797366647465	0.0	4.706449341661866E-5	2.353224670830933E-4
86-87	0.6783405436137248	0.001929644230081365	0.0	4.706449341661866E-5	2.353224670830933E-4
88	0.7729872398745449	0.001929644230081365	0.0	4.706449341661866E-5	2.353224670830933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2720	0.0	86.36039	1
AACGAGT	38875	0.0	73.9625	1
GTGGAAT	46150	0.0	73.79802	6
TGGAATC	46500	0.0	73.15652	7
GAATCAC	46640	0.0	73.148544	9
ACGAGTG	42680	0.0	73.066826	2
GGAATCA	46740	0.0	72.96187	8
CGAGTGG	44230	0.0	72.80741	3
GAGTGGA	46100	0.0	71.74885	4
AGTGGAA	46150	0.0	71.681305	5
ATCGAGT	1045	0.0	71.174385	1
AACGTGT	1275	0.0	69.78058	1
ACGTGTG	1370	0.0	69.65378	2
CGTGTGG	1505	0.0	67.77379	3
TCGAGTG	1155	0.0	67.153885	2
TGAGTGG	1910	0.0	50.695847	3
GCGAGTG	625	0.0	50.392277	2
ATGAGTG	1820	0.0	48.815712	2
AGCGAGT	610	0.0	48.61762	1
AATGAGT	1920	0.0	43.396526	1
>>END_MODULE