FastQCFastQC Report
Thu 26 May 2016
SRR2132765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750178
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1772136.4436920082991No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA993813.6136206456454816No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137150.4986949935604168No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG93590.34030524569682397No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81910.2978352673899653No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58470.2126044205138722No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47700.1734433189415376No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42450.15435364547312938No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41790.15195380080852947No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC36860.13402768838962423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT31250.083.171891
AACGAGT458150.074.890821
GTGGAAT543500.074.073786
ACGAGTG500850.073.9864652
TGGAATC544900.073.824997
CGAGTGG519550.073.426553
GGAATCA548150.073.410338
GAATCAC548800.073.323399
GAGTGGA544150.071.8024754
AGTGGAA545650.071.544785
ATCGAGT12250.066.111941
AACGTGT15500.065.919751
ACGTGTG16950.063.2395362
TCGAGTG14350.058.9717672
CGTGTGG20600.054.997353
ATGAGTG21100.045.4539072
AATGAGT20650.042.6392021
AGTACTT287500.041.40747520-21
GAGTACT286450.040.6158420-21
TGAGTGG26350.040.4983333