##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132765_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2750178
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.843340685584714	31.0	25.0	33.0	16.0	34.0
2	28.083371694486683	31.0	25.0	33.0	16.0	34.0
3	29.151022951968926	31.0	28.0	34.0	16.0	34.0
4	34.69771592965983	35.0	35.0	37.0	32.0	37.0
5	33.77478330493517	35.0	35.0	37.0	28.0	37.0
6	35.21469301259773	37.0	35.0	37.0	32.0	37.0
7	35.62136450804275	37.0	35.0	37.0	33.0	37.0
8	35.88587029639536	37.0	35.0	37.0	35.0	37.0
9	37.84833381693839	39.0	38.0	39.0	35.0	39.0
10-11	37.834447261231816	39.0	38.0	39.0	35.0	39.0
12-13	37.80226843498857	39.0	38.0	39.0	35.0	39.0
14-15	39.19743667500795	41.0	39.0	41.0	36.0	41.0
16-17	39.15388622118277	40.0	39.0	41.0	36.0	41.0
18-19	38.93829363044865	40.0	38.5	41.0	35.5	41.0
20-21	38.83240502978353	40.0	39.0	41.0	35.0	41.0
22-23	38.687771300621264	40.0	38.5	41.0	35.0	41.0
24-25	38.53194847751673	40.0	38.0	41.0	35.0	41.0
26-27	38.31923206425184	40.0	38.0	41.0	34.5	41.0
28-29	38.141065414674976	40.0	38.0	41.0	34.0	41.0
30-31	38.02386809144717	40.0	38.0	41.0	34.0	41.0
32-33	37.86567124018882	40.0	38.0	41.0	33.5	41.0
34-35	37.756445582795	40.0	37.5	41.0	33.0	41.0
36-37	37.5922887536734	40.0	37.0	41.0	33.0	41.0
38-39	37.142613678096474	40.0	36.0	41.0	33.0	41.0
40-41	36.89087142723126	40.0	35.0	41.0	33.0	41.0
42-43	36.72918861979116	39.5	35.0	41.0	32.0	41.0
44-45	36.57623851983399	39.0	35.0	41.0	32.0	41.0
46-47	36.42815174145092	39.0	35.0	41.0	31.5	41.0
48-49	36.250754496618036	39.0	35.0	41.0	31.0	41.0
50-51	35.97438002194767	39.0	35.0	41.0	30.5	41.0
52-53	35.59504130278113	39.0	35.0	41.0	29.5	41.0
54-55	34.87406287883911	38.0	35.0	40.5	26.0	41.0
56-57	34.24207178589895	38.0	35.0	40.0	22.0	41.0
58-59	33.90263121150704	37.0	34.0	40.0	20.0	41.0
60-61	33.62982141519567	37.0	34.0	40.0	19.5	41.0
62-63	33.33247029828615	36.0	34.0	40.0	18.0	41.0
64-65	33.039354543596815	36.0	34.0	39.5	17.5	41.0
66-67	32.72317846335764	35.0	33.5	39.0	14.0	41.0
68-69	32.39972576320514	35.0	33.0	39.0	11.0	40.5
70-71	32.06790505923617	35.0	33.0	37.5	7.5	40.0
72-73	31.74966056742509	35.0	33.0	37.0	3.5	39.5
74-75	31.427649046716247	35.0	33.0	36.5	2.0	39.0
76-77	31.12317620895811	35.0	33.0	36.0	2.0	39.0
78-79	30.818770639573145	35.0	33.0	35.5	2.0	37.5
80-81	30.542498340107443	35.0	33.0	35.0	2.0	37.0
82-83	30.291438226907495	35.0	32.5	35.0	2.0	37.0
84-85	30.062815025063834	35.0	32.0	35.0	2.0	36.0
86-87	29.872180818841542	35.0	32.0	35.0	2.0	36.0
88-89	29.682076214703194	35.0	32.0	35.0	2.0	36.0
90-91	29.509111410243264	35.0	32.0	35.0	2.0	35.0
92-93	29.347209526074312	35.0	32.0	35.0	2.0	35.0
94-95	29.17145035703144	35.0	32.0	35.0	2.0	35.0
96-97	28.997727601631603	35.0	31.0	35.0	2.0	35.0
98-99	28.828745266670012	35.0	31.0	35.0	2.0	35.0
100	28.72661551361403	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2371645038121173
1101	2	2.3379330978326074
1101	3	1.1649015313785434
1101	4	0.6694765590588503
1101	5	2.2699251523114015
1101	6	1.0154041219313115
1101	7	0.5783242234353452
1101	8	0.4090770658331948
1101	9	0.3748121126800257
1101	10-11	0.4828281746155412
1101	12-13	0.42707270362883065
1101	14-15	0.5339992308441452
1101	16-17	0.4574942657714516
1101	18-19	0.4093654951559458
1101	20-21	0.42353067435558245
1101	22-23	0.3856203924961079
1101	24-25	0.3378766157131423
1101	26-27	0.28559931317479936
1101	28-29	0.3938855560189438
1101	30-31	0.464674164177012
1101	32-33	0.47487194994601367
1101	34-35	0.44391311367416364
1101	36-37	0.4416921769543549
1101	38-39	0.3994124945274464
1101	40-41	0.47145284982240554
1101	42-43	0.5460402363973103
1101	44-45	0.6189910973546588
1101	46-47	0.580211268103497
1101	48-49	0.5349405235325975
1101	50-51	0.6017666451620087
1101	52-53	0.7292324564220607
1101	54-55	0.7246628461262148
1101	56-57	0.6906952746117128
1101	58-59	0.6070333335878146
1101	60-61	0.5941683681862244
1101	62-63	0.6190648638272975
1101	64-65	0.5888458293634713
1101	66-67	0.580452254860397
1101	68-69	0.7079477746715597
1101	70-71	0.7001801570448336
1101	72-73	0.6825921689584682
1101	74-75	0.6322458154297195
1101	76-77	0.6171041702930822
1101	78-79	0.6712620512582035
1101	80-81	0.6450722262628723
1101	82-83	0.6486949066876875
1101	84-85	0.6542634600510198
1101	86-87	0.6649802513136862
1101	88-89	0.6135950555895846
1101	90-91	0.5469016422554986
1101	92-93	0.5717344347997475
1101	94-95	0.6221132567395884
1101	96-97	0.5732673356015745
1101	98-99	0.6708433480135412
1101	100	0.7091987079641875
1102	1	1.522621853331966
1102	2	3.0622486049606152
1102	3	3.2741627328272465
1102	4	1.461623273080562
1102	5	2.667101019799766
1102	6	1.2662143758665607
1102	7	0.7626840640451888
1102	8	0.5497287236723594
1102	9	0.4743791934971071
1102	10-11	0.5583427946614137
1102	12-13	0.4682237963424214
1102	14-15	0.6064612962749578
1102	16-17	0.4675183609518072
1102	18-19	0.46289621509291834
1102	20-21	0.3816578090452154
1102	22-23	0.28846259768206295
1102	24-25	0.3616731973222329
1102	26-27	0.3992973690603563
1102	28-29	0.43974446437785986
1102	30-31	0.46829094201253696
1102	32-33	0.48596344150625015
1102	34-35	0.5014989704787709
1102	36-37	0.5193616991551195
1102	38-39	0.5247785112997221
1102	40-41	0.5335202915773536
1102	42-43	0.5671554106374828
1102	44-45	0.549726322621126
1102	46-47	0.5602192551427265
1102	48-49	0.5552640836999032
1102	50-51	0.6680795927249505
1102	52-53	0.7101315474774026
1102	54-55	0.6748083556467179
1102	56-57	0.6189601380328256
1102	58-59	0.5192269746877116
1102	60-61	0.5314196820000276
1102	62-63	0.5460359197046074
1102	64-65	0.5104614955103912
1102	66-67	0.4621895725352161
1102	68-69	0.44173764283642214
1102	70-71	0.5113751862086175
1102	72-73	0.4422874239662242
1102	74-75	0.4808819085970626
1102	76-77	0.4067875091951727
1102	78-79	0.4721677732576772
1102	80-81	0.4147946186415119
1102	82-83	0.42646794071072236
1102	84-85	0.4471067583630699
1102	86-87	0.4569458003104856
1102	88-89	0.5294504112886926
1102	90-91	0.5205286346324947
1102	92-93	0.5509492093270225
1102	94-95	0.48137729412862384
1102	96-97	0.5477054584771963
1102	98-99	0.625528115198307
1102	100	0.646741247069226
1103	1	1.400951011529262
1103	2	3.0760208966140468
1103	3	3.265321204052551
1103	4	1.4072866234585106
1103	5	2.640271933062806
1103	6	1.2344984942048711
1103	7	0.7559727479046785
1103	8	0.5067307444117333
1103	9	0.39557075482594684
1103	10-11	0.531159631906533
1103	12-13	0.4938541360171982
1103	14-15	0.5998746883381045
1103	16-17	0.4581405323136778
1103	18-19	0.3997242398499523
1103	20-21	0.3471423696435707
1103	22-23	0.271553346348135
1103	24-25	0.3115011962741434
1103	26-27	0.3114556497247776
1103	28-29	0.3356561394080231
1103	30-31	0.3605621597759878
1103	32-33	0.41723572323464
1103	34-35	0.4457504334305611
1103	36-37	0.4155063646413737
1103	38-39	0.4313819918148667
1103	40-41	0.5208905712254435
1103	42-43	0.4835720323106045
1103	44-45	0.5152065658244567
1103	46-47	0.4946944484826261
1103	48-49	0.5621408007328768
1103	50-51	0.5725621405239707
1103	52-53	0.5892803656029173
1103	54-55	0.661420858028734
1103	56-57	0.6532715681128565
1103	58-59	0.6731157566066557
1103	60-61	0.6329710659484533
1103	62-63	0.6666368911681175
1103	64-65	0.685628583660673
1103	66-67	0.7425499906575581
1103	68-69	0.7216852782934566
1103	70-71	0.7090015618199956
1103	72-73	0.7417192150797369
1103	74-75	0.7684481582794547
1103	76-77	0.6917459530273504
1103	78-79	0.6183139275008322
1103	80-81	0.6337952756795602
1103	82-83	0.6445422435754864
1103	84-85	0.6469158501022534
1103	86-87	0.6514730802920639
1103	88-89	0.6651748701751785
1103	90-91	0.6454369272069691
1103	92-93	0.6598296442822402
1103	94-95	0.6821472792013523
1103	96-97	0.6905690526142756
1103	98-99	0.5786779278134375
1103	100	0.6229782055587165
1104	1	6.101693394923284
1104	2	5.131076777052236
1104	3	4.122709485389027
1104	4	1.847633294177605
1104	5	2.8525431159420194
1104	6	1.2985738544681311
1104	7	0.6974150767034146
1104	8	0.5263194786452345
1104	9	0.41210900060982425
1104	10-11	0.4758396866143926
1104	12-13	0.3987344578855172
1104	14-15	0.5253794133635665
1104	16-17	0.40268968027066876
1104	18-19	0.3629974620790577
1104	20-21	0.2831449624508835
1104	22-23	0.27846457553016535
1104	24-25	0.26158937423603135
1104	26-27	0.2833323675534274
1104	28-29	0.30352535648153633
1104	30-31	0.3413493544597941
1104	32-33	0.4050247171240926
1104	34-35	0.43441271746490884
1104	36-37	0.3927356254661518
1104	38-39	0.4626434713660217
1104	40-41	0.46989856915420347
1104	42-43	0.4758861972559316
1104	44-45	0.4797759571901494
1104	46-47	0.4416778710257887
1104	48-49	0.5258944521447546
1104	50-51	0.4665236855519552
1104	52-53	0.4117133550422025
1104	54-55	0.39946364497764364
1104	56-57	0.3156142783281837
1104	58-59	0.3734798190216466
1104	60-61	0.2772859794683882
1104	62-63	0.31793493643154846
1104	64-65	0.3683893699829568
1104	66-67	0.284082467351368
1104	68-69	0.2184272902143647
1104	70-71	0.23250849317064137
1104	72-73	0.23978497520191056
1104	74-75	0.3032913985006154
1104	76-77	0.2148067414133763
1104	78-79	0.22935254194363353
1104	80-81	0.21745388250528563
1104	82-83	0.23800727163296642
1104	84-85	0.293142114752456
1104	86-87	0.2876509502502067
1104	88-89	0.32723569233148453
1104	90-91	0.37735880340886396
1104	92-93	0.42829908123781024
1104	94-95	0.45121416875188913
1104	96-97	0.49600733460739477
1104	98-99	0.5193120211657565
1104	100	0.540618394447165
1105	1	1.7759399387588708
1105	2	3.2464547578143588
1105	3	3.3797459869757525
1105	4	1.4639655021525897
1105	5	2.6757312479293063
1105	6	1.2370474194993335
1105	7	0.7717352753798181
1105	8	0.5201315136819389
1105	9	0.4554437851351878
1105	10-11	0.5163115156913562
1105	12-13	0.4547079613095022
1105	14-15	0.5277774397424082
1105	16-17	0.42699091761723196
1105	18-19	0.3396405263394868
1105	20-21	0.32167014063261945
1105	22-23	0.26823442468833747
1105	24-25	0.28336505828140446
1105	26-27	0.3740549379704703
1105	28-29	0.37259148702857914
1105	30-31	0.3799204642383671
1105	32-33	0.32162053550056413
1105	34-35	0.36472223208225074
1105	36-37	0.41920180858468115
1105	38-39	0.5077687667383586
1105	40-41	0.5477833609355329
1105	42-43	0.5427364534050128
1105	44-45	0.5995726123294176
1105	46-47	0.7194475942314469
1105	48-49	0.652005024763227
1105	50-51	0.6897146240898664
1105	52-53	0.7503811196862316
1105	54-55	0.7995707046161584
1105	56-57	0.9379039718528546
1105	58-59	0.9638326777330448
1105	60-61	1.023816056107755
1105	62-63	1.0749220837987323
1105	64-65	1.0773483587485444
1105	66-67	0.9999626153181893
1105	68-69	1.0610173120370021
1105	70-71	1.0887966725270246
1105	72-73	1.0102954525345673
1105	74-75	1.0088175651358
1105	76-77	0.9365152051486376
1105	78-79	1.0056267159278676
1105	80-81	0.9988164012270282
1105	82-83	1.0026864212891944
1105	84-85	0.9885061038329255
1105	86-87	1.0155978284075786
1105	88-89	1.009674557444555
1105	90-91	1.0521490650889085
1105	92-93	1.1585202921891096
1105	94-95	1.189167632035364
1105	96-97	1.1291023849473305
1105	98-99	1.1233746743281223
1105	100	1.1384647433301573
1106	1	-0.5636354760923403
1106	2	2.025658119885989
1106	3	2.824345968434919
1106	4	1.2672935822490032
1106	5	2.5041512596718363
1106	6	1.1471645357812719
1106	7	0.639829586433251
1106	8	0.45348338785146325
1106	9	0.353496997335057
1106	10-11	0.48133886342473886
1106	12-13	0.406033631097209
1106	14-15	0.47821469446259357
1106	16-17	0.38423111415009714
1106	18-19	0.3208802902528376
1106	20-21	0.35022457343753643
1106	22-23	0.2408063476820672
1106	24-25	0.22705499369749305
1106	26-27	0.24191130829277085
1106	28-29	0.3109864686422483
1106	30-31	0.3273710992620096
1106	32-33	0.2895665198644579
1106	34-35	0.3870716650416668
1106	36-37	0.3867074480890267
1106	38-39	0.3870802836877729
1106	40-41	0.3197109898715951
1106	42-43	0.3474659117035799
1106	44-45	0.4652597048172922
1106	46-47	0.44102449234955543
1106	48-49	0.4673892835932989
1106	50-51	0.43728268440938933
1106	52-53	0.46591679534520836
1106	54-55	0.47330915522028505
1106	56-57	0.4636046609539335
1106	58-59	0.4725468707431446
1106	60-61	0.42567941014502253
1106	62-63	0.44850293729522406
1106	64-65	0.4967837902865071
1106	66-67	0.5231803774392674
1106	68-69	0.5093749029643604
1106	70-71	0.4889388189378323
1106	72-73	0.5075359580813661
1106	74-75	0.47690569591049226
1106	76-77	0.45984421228685335
1106	78-79	0.5294022482043701
1106	80-81	0.556853853716138
1106	82-83	0.5801593053162648
1106	84-85	0.5564134614547527
1106	86-87	0.5711199074533404
1106	88-89	0.6106587668324011
1106	90-91	0.5731805536090384
1106	92-93	0.5074557391777681
1106	94-95	0.5341291598002655
1106	96-97	0.4819638539995843
1106	98-99	0.5125483710618468
1106	100	0.45704575133362013
1107	1	-0.8915135083194556
1107	2	1.7266146280831194
1107	3	-0.6765455548836918
1107	4	-0.15640253846136432
1107	5	1.8300581159610445
1107	6	0.7174586596486066
1107	7	0.3032893390677316
1107	8	0.2510589519990347
1107	9	0.27316107550148416
1107	10-11	0.2793361082966044
1107	12-13	0.2515427049473331
1107	14-15	0.31550308504863267
1107	16-17	0.23399948535602988
1107	18-19	0.21509404051479208
1107	20-21	0.13142010293932316
1107	22-23	0.2065637868164174
1107	24-25	0.10633356541445949
1107	26-27	0.09876298319149157
1107	28-29	0.11927718368740159
1107	30-31	0.10826713114689568
1107	32-33	0.11445808355829712
1107	34-35	0.1167843184434787
1107	36-37	0.27680355072667595
1107	38-39	0.2625714674700177
1107	40-41	0.23254310009210144
1107	42-43	0.2450453211102399
1107	44-45	0.32516057403810095
1107	46-47	0.3119368377461029
1107	48-49	0.22036546605853147
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2216	90-91	0.07600236074714317
2216	92-93	0.045363165195993815
2216	94-95	-0.019524093496492867
2216	96-97	-7.547894754900142E-4
2216	98-99	-0.057706643673395064
2216	100	-0.06979793627927222
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1375.0
3	343.0
4	469.0
5	1267.0
6	3599.0
7	6268.0
8	7850.0
9	10440.0
10	7474.0
11	6258.0
12	5535.0
13	7361.0
14	7622.0
15	8144.0
16	9271.0
17	10674.0
18	14480.0
19	24134.0
20	46199.0
21	53654.0
22	25860.0
23	17812.0
24	19093.0
25	20853.0
26	22588.0
27	25425.0
28	29937.0
29	35304.0
30	41916.0
31	51663.0
32	66813.0
33	94826.0
34	140242.0
35	289399.0
36	395446.0
37	608162.0
38	530306.0
39	101937.0
40	179.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.773127362744631	39.82631244172215	24.109743855999234	21.29081633953398
2	15.997773122490264	39.20649852602091	24.394272226684784	20.40145612480405
3	19.80964910462572	25.647990475909964	18.576557255264305	35.96580316420001
4	31.325536019850354	27.310995870085502	20.813125550418917	20.55034255964523
5	18.330929789615382	37.79359777404944	21.16900221300561	22.70647022332957
6	35.498977944623185	24.13472584514579	22.554167480347118	17.81212872988391
7	20.140213593009683	23.557810835281753	36.57603932962194	19.72593624208662
8	34.409918194385966	23.91728826279608	22.307210660546335	19.36558288227162
9	34.064086033704	25.36803072382951	22.337535970399006	18.230347272067483
10-11	18.13355353726195	40.85108309353067	22.24901442742979	18.766348941777586
12-13	18.030196590911572	25.441625960210573	29.7863083771305	26.741869071747352
14-15	18.31108750051815	32.832765733708875	22.191254529706804	26.664892236066173
16-17	26.17303316367159	25.077049558246777	29.9313898954904	18.818527382591235
18-19	18.48413084534892	25.215549684420424	30.07956212288805	26.22075734734261
20-21	26.290352842616006	33.089512751538265	22.254414077925137	18.36572032792059
22-23	25.838073026545917	25.502585650819693	30.12892256428493	18.53041875834946
24-25	18.365429437658218	33.140236741040034	22.081661623356744	26.412672197945003
26-27	18.833787788931403	28.374573709255806	34.039609086978764	18.752029414834027
28-29	25.199805248969337	25.421827241727623	30.791116065941914	18.587251443361122
30-31	23.89736227982334	26.20754002104591	30.920125897305557	18.97497180182519
32-33	20.63837322529669	27.473676249319134	31.574319916747207	20.313630608636966
34-35	20.355118832308307	27.29357881562575	32.009819000806495	20.341483351259445
36-37	20.480256187054074	27.301141962447524	32.06645169876277	20.152150151735633
38-39	21.087198719501068	26.98403521517516	31.754580976213177	20.174185089110598
40-41	20.187420596048693	27.823599054315757	31.73449136746785	20.2544889821677
42-43	20.309164673595372	27.37115487232072	31.99868007319594	20.32100038088797
44-45	20.363427385427414	27.646628690942915	31.428947508124928	20.560996415504743
46-47	20.674625424245267	27.22918298379232	31.775106920352066	20.321084671610347
48-49	20.310339912543842	27.43024269701816	31.640606535286082	20.61881085515192
50-51	20.628846569203883	27.82378086072974	31.038336427678498	20.509036142387874
52-53	20.755672323946108	27.732304889127906	30.939157111360426	20.57286567556556
54-55	21.080035546790064	28.4946283476924	29.37353509481932	21.051801010698217
56-57	21.30323928123925	29.14816786404371	28.264734137208574	21.283858717508465
58-59	21.85638529578813	29.537288131895462	27.330012820988316	21.276313751328097
60-61	21.604183438308358	29.864030619109016	27.085101400709338	21.44668454187329
62-63	21.077119959391695	29.867919386264845	26.90532771260317	22.149632941740286
64-65	21.22153547879446	29.820378899111255	27.56654296558259	21.39154265651169
66-67	21.249228458890382	29.444372226883537	27.959486251341957	21.346913062884123
68-69	21.463447093242692	29.2814137848532	27.937246243697683	21.31789287820643
70-71	20.569014078361473	29.906191526512103	28.201047350389686	21.32374704473674
72-73	20.613167515222738	29.601492566619715	28.225537259259635	21.55980265889791
74-75	20.753234395081172	29.874191253511377	27.72233972015876	21.65023463124869
76-77	21.1718486094804	29.565217865585225	27.661200679669797	21.601732845264582
78-79	21.106144138208386	29.811400475462207	27.144143298986318	21.938312087343093
80-81	21.40579604748832	30.275639510572148	26.745886588004293	21.57267785393524
82-83	21.17297623274662	30.48783347959811	26.61562580506987	21.7235644825854
84-85	21.26100738416282	30.56943412932239	26.19818791208092	21.97137057443387
86-87	21.29402943883248	30.620974577272662	26.306197746545042	21.778798237349815
88-89	21.794043876432724	30.199881607663215	26.287643927047633	21.718430588856428
90-91	21.43796173156096	30.659457435020105	26.17111077375915	21.731470059659785
92-93	21.103597658042496	30.74593717206668	26.193559107810476	21.956906062080346
94-95	21.13620323346461	30.91271943587631	25.91351393019431	22.037563400464773
96-97	21.20910208207582	30.513428407281083	25.99880369726632	22.278665813376776
98-99	21.78232636409865	30.49098806486972	25.484058464266695	22.242627106764935
100	21.262405560658255	31.030282403538973	25.3633764796315	22.343935556171274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104741.0
1	56975.5
2	6745.5
3	3708.0
4	3060.5
5	2869.5
6	2920.5
7	3247.5
8	3195.5
9	2938.0
10	2885.5
11	4340.0
12	29081.0
13	32178.0
14	10198.5
15	8406.5
16	8807.5
17	9437.5
18	10142.0
19	11010.0
20	11763.5
21	12760.0
22	14000.0
23	15285.0
24	16704.5
25	17978.5
26	19136.0
27	20642.0
28	22633.5
29	24842.5
30	27168.0
31	29415.5
32	31930.5
33	34807.5
34	37867.5
35	41584.5
36	45643.5
37	49685.0
38	53510.5
39	56199.5
40	58245.0
41	60548.0
42	63046.0
43	66515.0
44	70644.5
45	74634.0
46	79839.5
47	86380.5
48	90698.5
49	94369.0
50	104808.5
51	106951.5
52	97466.0
53	94367.5
54	94556.5
55	94319.0
56	90172.0
57	82807.5
58	74057.0
59	62613.5
60	52024.5
61	43086.5
62	35441.0
63	28132.0
64	22422.5
65	17480.0
66	13224.0
67	10574.5
68	8499.5
69	6768.0
70	5079.0
71	3632.5
72	2589.5
73	1900.5
74	1368.5
75	983.0
76	717.0
77	489.0
78	308.0
79	167.5
80	76.5
81	28.5
82	21.5
83	17.0
84	10.0
85	8.5
86	5.0
87	2.0
88	3.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1322459855325728
2	0.005054218308778559
3	0.003599766996899837
4	0.0
5	0.0026180123613816996
6	0.011890139474608553
7	0.0031997928861331884
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.636128279696805E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.272127113226853E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.6362577258635622E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.454192419545207E-5
64-65	0.0
66-67	1.8180641398484026E-5
68-69	0.0
70-71	0.0
72-73	5.817805247514888E-4
74-75	4.1815475216513254E-4
76-77	6.90864373142393E-4
78-79	4.363353935636166E-4
80-81	0.0010181159183151054
82-83	0.0010362965597135894
84-85	0.0012908255392923658
86-87	3.636128279696805E-5
88-89	0.0
90-91	1.8180641398484026E-5
92-93	0.0
94-95	3.636128279696805E-5
96-97	0.001381728746284786
98-99	7.090450145408769E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2750178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.00623254435479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.90474656229121	57.14356396780874
2	7.982916457003689	10.378786471722774
3	1.8362140310649884	3.5809606891385326
4	0.7367933702848949	1.9158464466351512
5	0.37141219199810277	1.207205366141861
6	0.22160779550256088	0.8643532732848778
7	0.1557700760283119	0.7088218050023788
8	0.10569429849731969	0.5496630517383371
9	0.07653314302241009	0.44776181633986395
>10	0.5638173436053897	6.544458800462613
>50	0.026044014973337713	1.152563447040019
>100	0.01553878242857544	2.0449313784587
>500	0.0017919589533922768	0.7680232444245684
>1k	7.839820421068303E-4	1.2733285177107723
>5k	1.679961518800351E-4	0.8517129514531273
>10k+	1.679961518800351E-4	10.568018772637771
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	177213	6.4436920082991	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99381	3.6136206456454816	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13715	0.4986949935604168	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	9359	0.34030524569682397	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8191	0.2978352673899653	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5847	0.2126044205138722	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4770	0.1734433189415376	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4245	0.15435364547312938	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4179	0.15195380080852947	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3686	0.13402768838962423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.363095043302652E-4	3.636128279696805E-5	0.0	0.0	0.0
2	8.363095043302652E-4	3.636128279696805E-5	0.0	0.0	0.0
3	8.363095043302652E-4	3.636128279696805E-5	0.0	0.0	0.0
4	8.363095043302652E-4	3.636128279696805E-5	0.0	0.0	0.0
5	8.363095043302652E-4	7.27225655939361E-5	0.0	0.0	0.0
6	8.363095043302652E-4	7.27225655939361E-5	0.0	0.0	0.0
7	8.726707871272333E-4	7.27225655939361E-5	0.0	0.0	0.0
8	8.726707871272333E-4	7.27225655939361E-5	0.0	0.0	0.0
9	9.090320699242012E-4	7.27225655939361E-5	0.0	3.636128279696805E-5	0.0
10-11	9.090320699242012E-4	9.090320699242013E-5	0.0	3.636128279696805E-5	0.0
12-13	9.999352769166213E-4	1.0908384839090416E-4	0.0	3.636128279696805E-5	0.0
14-15	0.0010362965597135894	1.0908384839090416E-4	0.0	3.636128279696805E-5	0.0
16-17	0.0011090191253075256	1.0908384839090416E-4	0.0	5.454192419545208E-5	0.0
18-19	0.0011271997667060096	1.8180641398484026E-4	0.0	1.0908384839090416E-4	0.0
20-21	0.0011453804081044937	1.9998705538332428E-4	0.0	1.0908384839090416E-4	0.0
22-23	0.0011635610495029777	2.1816769678180832E-4	0.0	1.0908384839090416E-4	0.0
24-25	0.0012181029736984296	2.3634833818029233E-4	0.0	1.0908384839090416E-4	1.8180641398484026E-5
26-27	0.0012544642564953977	2.5452897957877634E-4	0.0	1.0908384839090416E-4	3.636128279696805E-5
28-29	0.0013453674634878179	3.0907090377422846E-4	0.0	1.0908384839090416E-4	3.636128279696805E-5
30-31	0.001436270670480238	3.454321865711965E-4	0.0	1.0908384839090416E-4	7.27225655939361E-5
32-33	0.0015635351602696261	3.6361282796968053E-4	0.0	1.0908384839090416E-4	7.27225655939361E-5
34-35	0.0019089673468408227	3.6361282796968053E-4	0.0	1.0908384839090416E-4	7.27225655939361E-5
36-37	0.0026543736441786677	3.817934693681645E-4	0.0	1.0908384839090416E-4	7.27225655939361E-5
38-39	0.004345173294237682	4.3633539356361664E-4	0.0	1.0908384839090416E-4	7.27225655939361E-5
40-41	0.006835921165829994	4.7269667636058466E-4	0.0	1.0908384839090416E-4	1.0908384839090416E-4
42-43	0.009781185072384405	5.454192419545208E-4	0.0	1.0908384839090416E-4	1.0908384839090416E-4
44-45	0.013417313352081212	5.817805247514888E-4	0.0	1.0908384839090416E-4	1.0908384839090416E-4
46-47	0.01716252548016892	6.181418075484568E-4	0.0	1.0908384839090416E-4	1.0908384839090416E-4
48-49	0.02163496326419599	6.363224489469409E-4	0.0	1.0908384839090416E-4	1.0908384839090416E-4
50-51	0.02728914273912452	6.726837317439089E-4	0.0	1.0908384839090416E-4	1.454451311878722E-4
52-53	0.03468866378830752	7.63586938736329E-4	0.0	1.0908384839090416E-4	2.1816769678180832E-4
54-55	0.04348809422517379	7.817675801348131E-4	0.0	1.0908384839090416E-4	2.1816769678180832E-4
56-57	0.05321473737336274	8.181288629317812E-4	0.0	1.0908384839090416E-4	2.727096209772604E-4
58-59	0.063741328743085	8.544901457287492E-4	0.0	1.0908384839090416E-4	3.0907090377422846E-4
60-61	0.07792222903390253	8.726707871272333E-4	0.0	1.0908384839090416E-4	3.2725154517271245E-4
62-63	0.09648466390175472	8.726707871272333E-4	0.0	1.0908384839090416E-4	3.2725154517271245E-4
64-65	0.11784691754497345	8.726707871272333E-4	0.0	1.0908384839090416E-4	3.2725154517271245E-4
66-67	0.14069998378286785	9.090320699242014E-4	0.0	1.0908384839090416E-4	3.2725154517271245E-4
68-69	0.17020716477260744	9.453933527211693E-4	0.0	1.0908384839090416E-4	3.2725154517271245E-4
70-71	0.20931372442074658	9.635739941196533E-4	0.0	1.0908384839090416E-4	3.454321865711965E-4
72-73	0.25385629584703245	9.817546355181373E-4	0.0	1.0908384839090416E-4	3.6361282796968053E-4
74-75	0.30161684080084994	9.999352769166213E-4	0.0	1.0908384839090416E-4	3.6361282796968053E-4
76-77	0.35921311275124734	0.0010181159183151054	0.0	1.0908384839090416E-4	3.6361282796968053E-4
78-79	0.42629967951165343	0.0010181159183151054	0.0	1.0908384839090416E-4	3.6361282796968053E-4
80-81	0.5101669782828602	0.0010181159183151054	0.0	1.0908384839090416E-4	3.9997411076664856E-4
82-83	0.6097059899395603	0.0010181159183151054	0.0	1.0908384839090416E-4	4.3633539356361664E-4
84-85	0.7326071257933123	0.0010544772011120735	0.0	1.0908384839090416E-4	4.7269667636058466E-4
86-87	0.8686346847367697	0.0010544772011120735	0.0	1.0908384839090416E-4	4.908773177590686E-4
88	0.9833545319612039	0.0010544772011120735	0.0	1.0908384839090416E-4	5.090579591575527E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3125	0.0	83.17189	1
AACGAGT	45815	0.0	74.89082	1
GTGGAAT	54350	0.0	74.07378	6
ACGAGTG	50085	0.0	73.986465	2
TGGAATC	54490	0.0	73.82499	7
CGAGTGG	51955	0.0	73.42655	3
GGAATCA	54815	0.0	73.41033	8
GAATCAC	54880	0.0	73.32339	9
GAGTGGA	54415	0.0	71.802475	4
AGTGGAA	54565	0.0	71.54478	5
ATCGAGT	1225	0.0	66.11194	1
AACGTGT	1550	0.0	65.91975	1
ACGTGTG	1695	0.0	63.239536	2
TCGAGTG	1435	0.0	58.971767	2
CGTGTGG	2060	0.0	54.99735	3
ATGAGTG	2110	0.0	45.453907	2
AATGAGT	2065	0.0	42.639202	1
AGTACTT	28750	0.0	41.407475	20-21
GAGTACT	28645	0.0	40.61584	20-21
TGAGTGG	2635	0.0	40.498333	3
>>END_MODULE