FastQCFastQC Report
Thu 26 May 2016
SRR2132764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755799
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1338997.626100709705382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA799104.5512043235017225No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG121460.6917648318514819No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105490.600809090334372No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64480.3672402137146678No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC48860.27827786665785775No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45540.25936909634872785No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41890.23858083983417233No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37440.21323625312464584No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31080.17701342807462586No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA26900.15320660280590204No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19670.11202876866885104No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.10787111736593996No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.10001144777961486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT25850.087.060171
CGTGTGG14700.071.627083
AACGTGT14400.069.968641
GTGGAAT463400.068.882976
ATCGAGT9200.068.575521
AACGAGT391500.068.103111
TGGAATC469150.068.0309757
GAATCAC471400.067.6863259
GGAATCA471700.067.623358
ACGAGTG429150.067.615472
CGAGTGG444950.067.1669853
ACGTGTG15300.066.976742
GAGTGGA463850.066.323324
AGTGGAA465950.066.024415
TCGAGTG10250.063.286942
ATGAGTG16700.055.7323882
TGAGTGG18350.055.0742953
GCGAGTG5200.052.430452
AATGAGT17000.050.682071
GTGTGGA24700.046.242734
Produced by FastQC (version 0.11.5)