##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132764_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1755799
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.968338061475148	31.0	25.0	33.0	16.0	34.0
2	28.226034984642318	31.0	25.0	34.0	16.0	34.0
3	29.29511692397592	31.0	28.0	34.0	16.0	34.0
4	34.797993392182136	35.0	35.0	37.0	32.0	37.0
5	33.85745122306141	35.0	35.0	37.0	28.0	37.0
6	35.284720517553545	37.0	35.0	37.0	32.0	37.0
7	35.66874055629374	37.0	35.0	37.0	33.0	37.0
8	35.94344170374855	37.0	35.0	37.0	35.0	37.0
9	37.91117149514267	39.0	38.0	39.0	35.0	39.0
10-11	37.899983141578275	39.0	38.0	39.0	35.0	39.0
12-13	37.874774105692055	39.0	38.0	39.0	35.0	39.0
14-15	39.2904706062596	41.0	39.0	41.0	36.0	41.0
16-17	39.213777602105935	40.5	39.0	41.0	36.0	41.0
18-19	38.938321527691954	40.0	38.5	41.0	35.5	41.0
20-21	38.789883124435086	40.0	39.0	41.0	35.0	41.0
22-23	38.6064663438127	40.0	38.0	41.0	35.0	41.0
24-25	38.408673487113276	40.0	38.0	41.0	35.0	41.0
26-27	38.184339722257505	40.0	38.0	41.0	34.5	41.0
28-29	38.02762161272446	40.0	38.0	41.0	34.0	41.0
30-31	37.890935693664254	40.0	38.0	41.0	34.0	41.0
32-33	37.73480592026764	40.0	37.5	41.0	33.0	41.0
34-35	37.63901448856048	40.0	37.0	41.0	33.0	41.0
36-37	37.4579610194561	40.0	37.0	41.0	33.0	41.0
38-39	36.94819110843554	40.0	35.5	41.0	33.0	41.0
40-41	36.69174575221879	39.5	35.0	41.0	32.5	41.0
42-43	36.525577244320104	39.0	35.0	41.0	32.0	41.0
44-45	36.37692013721389	39.0	35.0	41.0	32.0	41.0
46-47	36.23801841782573	39.0	35.0	41.0	31.0	41.0
48-49	36.057702219901024	39.0	35.0	41.0	31.0	41.0
50-51	35.75641716392366	39.0	35.0	41.0	30.5	41.0
52-53	35.268361583529774	38.5	35.0	41.0	28.0	41.0
54-55	34.34992986099206	38.0	35.0	40.5	22.0	41.0
56-57	33.60986992246835	38.0	34.0	40.0	10.0	41.0
58-59	33.25297371737881	37.0	34.0	40.0	3.5	41.0
60-61	32.99354368011373	37.0	34.0	40.0	2.0	41.0
62-63	32.727682667549075	36.0	34.0	40.0	2.0	41.0
64-65	32.448756093379714	36.0	33.5	40.0	2.0	41.0
66-67	32.13699745813729	35.0	33.0	39.0	2.0	41.0
68-69	31.837829387076766	35.0	33.0	39.0	2.0	41.0
70-71	31.53201363026178	35.0	33.0	38.5	2.0	40.0
72-73	31.233715533497858	35.0	33.0	37.0	2.0	40.0
74-75	30.923625938959983	35.0	33.0	37.0	2.0	39.5
76-77	30.613601841668665	35.0	33.0	36.5	2.0	39.0
78-79	30.293590838131244	35.0	32.5	36.0	2.0	39.0
80-81	30.00492966450032	35.0	32.0	35.5	2.0	37.5
82-83	29.725910255103233	35.0	32.0	35.0	2.0	37.0
84-85	29.46087422307451	35.0	32.0	35.0	2.0	37.0
86-87	29.268450147197942	35.0	31.0	35.0	2.0	36.0
88-89	29.06203557468708	35.0	31.0	35.0	2.0	36.0
90-91	28.87020239788267	35.0	31.0	35.0	2.0	36.0
92-93	28.708639200728555	35.0	31.0	35.0	2.0	35.5
94-95	28.52090216476943	35.0	31.0	35.0	2.0	35.0
96-97	28.318541017508267	35.0	31.0	35.0	2.0	35.0
98-99	28.148459761054653	34.5	30.5	35.0	2.0	35.0
100	28.04835519327668	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.059382950883964725
1101	2	2.278563237463967
1101	3	1.181656312406556
1101	4	0.667149486004682
1101	5	2.1976871401816496
1101	6	0.9571019775148883
1101	7	0.554421873355885
1101	8	0.40109495573149445
1101	9	0.2998094850491384
1101	10-11	0.45222749769084203
1101	12-13	0.42123792191669196
1101	14-15	0.5406287116668409
1101	16-17	0.5208582235777897
1101	18-19	0.45586282408486056
1101	20-21	0.4345790175789972
1101	22-23	0.4028303022873203
1101	24-25	0.37205440507221965
1101	26-27	0.30211117742639004
1101	28-29	0.3482973509150753
1101	30-31	0.32466340147734485
1101	32-33	0.3299230983103527
1101	34-35	0.36170613705824906
1101	36-37	0.378858313495158
1101	38-39	0.3847656133657722
1101	40-41	0.3550905627528991
1101	42-43	0.37664971277651915
1101	44-45	0.42893601106385404
1101	46-47	0.37414608743717537
1101	48-49	0.40375391662779947
1101	50-51	0.42736539231034243
1101	52-53	0.6331876713498161
1101	54-55	0.5700002757136318
1101	56-57	0.2988611519039992
1101	58-59	0.33959579389883743
1101	60-61	0.2685861008273136
1101	62-63	0.3054520180263509
1101	64-65	0.2754752326317984
1101	66-67	0.19932660991345585
1101	68-69	0.2679333908054744
1101	70-71	0.15896232146365818
1101	72-73	0.2627506787556442
1101	74-75	0.26665430947621616
1101	76-77	0.1612885669051458
1101	78-79	0.2298259738131989
1101	80-81	0.30040340486704764
1101	82-83	0.2844621868934567
1101	84-85	0.24021988381646509
1101	86-87	0.2727664686428106
1101	88-89	0.26588889337268995
1101	90-91	0.2885976901960632
1101	92-93	0.25060974942687153
1101	94-95	0.23025603354355084
1101	96-97	0.26754318500634255
1101	98-99	0.33147460988076105
1101	100	0.34298929960543845
1102	1	1.5146609106490665
1102	2	3.002358884389487
1102	3	3.1870743275290074
1102	4	1.40552092169969
1102	5	2.5779095829293652
1102	6	1.1519146501604283
1102	7	0.6453335899246753
1102	8	0.4519687057098949
1102	9	0.34629930343652404
1102	10-11	0.4268703574785917
1102	12-13	0.38013908782180295
1102	14-15	0.46815276169979825
1102	16-17	0.3023386490603386
1102	18-19	0.2091056596806382
1102	20-21	0.17346033618978396
1102	22-23	0.10929949578115838
1102	24-25	0.17057462141760027
1102	26-27	0.15328053853605894
1102	28-29	0.2962607815347127
1102	30-31	0.3143906986658962
1102	32-33	0.284369484695695
1102	34-35	0.2987214644028313
1102	36-37	0.2536821811008707
1102	38-39	0.24629025091813617
1102	40-41	0.31613321221858115
1102	42-43	0.4075084291700293
1102	44-45	0.32415254158894413
1102	46-47	0.39167144581167435
1102	48-49	0.30912435008382033
1102	50-51	0.46796613090617
1102	52-53	0.5594780540520645
1102	54-55	0.5056630124185304
1102	56-57	0.5010021417507744
1102	58-59	0.4773766861877675
1102	60-61	0.3874291195909052
1102	62-63	0.33227616704891716
1102	64-65	0.3443376296616165
1102	66-67	0.4458827093770381
1102	68-69	0.4738204957418688
1102	70-71	0.5138228720039031
1102	72-73	0.45990571475972075
1102	74-75	0.47017387165082525
1102	76-77	0.48899868532239665
1102	78-79	0.5076288487085918
1102	80-81	0.46084915436160756
1102	82-83	0.5106488896999721
1102	84-85	0.48882736407408345
1102	86-87	0.42062868793566466
1102	88-89	0.496100104131699
1102	90-91	0.4336979511238006
1102	92-93	0.4886175649161544
1102	94-95	0.46709350049558296
1102	96-97	0.49390348992398003
1102	98-99	0.4877270013069328
1102	100	0.5051429285252169
1103	1	1.630130934970957
1103	2	3.1640023858176143
1103	3	3.308357639099146
1103	4	1.448178218874908
1103	5	2.578394758105837
1103	6	1.2172305691236645
1103	7	0.6814304801102224
1103	8	0.5005444729668511
1103	9	0.44041268600447125
1103	10-11	0.4542918080582652
1103	12-13	0.44499169010228457
1103	14-15	0.5980216369438978
1103	16-17	0.4725016622018714
1103	18-19	0.4885801409910897
1103	20-21	0.454914113039699
1103	22-23	0.47840190145431905
1103	24-25	0.5044440536661554
1103	26-27	0.607283239697928
1103	28-29	0.5683288070730583
1103	30-31	0.6011193410116178
1103	32-33	0.5900072726590224
1103	34-35	0.5615859920104072
1103	36-37	0.562304874434183
1103	38-39	0.5449611949406119
1103	40-41	0.647812685461048
1103	42-43	0.6635721063571296
1103	44-45	0.672634585342621
1103	46-47	0.7263327273078559
1103	48-49	0.7584843548295552
1103	50-51	0.7711102042287266
1103	52-53	0.7760801815803546
1103	54-55	0.6551914883776462
1103	56-57	0.6943327758281512
1103	58-59	0.5495427116322489
1103	60-61	0.6535348389402316
1103	62-63	0.6206904149273385
1103	64-65	0.6755265673340602
1103	66-67	0.6217871804932145
1103	68-69	0.5638917842113287
1103	70-71	0.4990270541591322
1103	72-73	0.6004328987448702
1103	74-75	0.5996621589526718
1103	76-77	0.6095996187932435
1103	78-79	0.6058253621656817
1103	80-81	0.637494729666443
1103	82-83	0.6513574932269179
1103	84-85	0.646661114684278
1103	86-87	0.661046865339916
1103	88-89	0.6433343912100753
1103	90-91	0.5906882140284715
1103	92-93	0.6042395861752397
1103	94-95	0.6307620990480878
1103	96-97	0.6162445262619656
1103	98-99	0.6350702234942922
1103	100	0.6541387600449511
1104	1	6.021116348432425
1104	2	5.074425647388779
1104	3	4.062107595333348
1104	4	1.8114398037664756
1104	5	2.8336379002633123
1104	6	1.2864179395854975
1104	7	0.6825886431688701
1104	8	0.5032909052822774
1104	9	0.38458366574725744
1104	10-11	0.39714380690216444
1104	12-13	0.3774672481933976
1104	14-15	0.4580085106382228
1104	16-17	0.3657706203425519
1104	18-19	0.2770931818247462
1104	20-21	0.22829242731174304
1104	22-23	0.16752883049606027
1104	24-25	0.19245212304883808
1104	26-27	0.31750665463815864
1104	28-29	0.23936879218925355
1104	30-31	0.32749387872468816
1104	32-33	0.39586169423998996
1104	34-35	0.34841911286510197
1104	36-37	0.4250730214488101
1104	38-39	0.33366834994430405
1104	40-41	0.3457885432191219
1104	42-43	0.4532970306165609
1104	44-45	0.5059510988805016
1104	46-47	0.42074759408478
1104	48-49	0.45176337052139814
1104	50-51	0.4339004229341228
1104	52-53	0.4148642614036646
1104	54-55	0.4197646397276529
1104	56-57	0.5041621613152145
1104	58-59	0.5294004916801285
1104	60-61	0.4046487213128813
1104	62-63	0.5099696489332501
1104	64-65	0.4599369511206035
1104	66-67	0.4604137721092627
1104	68-69	0.43501851141552805
1104	70-71	0.46893283584367396
1104	72-73	0.450886895917769
1104	74-75	0.4404284688337192
1104	76-77	0.3877030941748991
1104	78-79	0.26330584605403473
1104	80-81	0.31351045651845766
1104	82-83	0.3099738282003557
1104	84-85	0.23165411558979798
1104	86-87	0.2505690252487973
1104	88-89	0.2457899440424285
1104	90-91	0.3068965584319763
1104	92-93	0.3065271470096924
1104	94-95	0.30751120330816306
1104	96-97	0.24690876401286488
1104	98-99	0.2829620509273063
1104	100	0.3711638943115432
1105	1	1.6951453491828339
1105	2	3.0021154378300317
1105	3	3.17385175902697
1105	4	1.2873869697572502
1105	5	2.4975842900390077
1105	6	1.1015292601731232
1105	7	0.6290910769123528
1105	8	0.41760451368086393
1105	9	0.2829649708321824
1105	10-11	0.3827512014534875
1105	12-13	0.3805585804469871
1105	14-15	0.41286423728773514
1105	16-17	0.2793852600226714
1105	18-19	0.18712203297072705
1105	20-21	0.12739964355414202
1105	22-23	-0.009251936831418561
1105	24-25	0.08639542865419969
1105	26-27	0.08168210061794667
1105	28-29	0.027358966952462538
1105	30-31	0.05956854543137524
1105	32-33	0.004841529586435911
1105	34-35	0.033669525783551535
1105	36-37	0.08130290343296309
1105	38-39	0.10613746305263305
1105	40-41	0.10210344735576626
1105	42-43	0.14744714501961198
1105	44-45	0.09198097815904305
1105	46-47	0.1153032660877571
1105	48-49	0.05749215156019005
1105	50-51	0.11571203708065525
1105	52-53	0.16551915024564323
1105	54-55	0.25948622997727
1105	56-57	0.2813942315483544
1105	58-59	0.31125660063943883
1105	60-61	0.29079491917201494
1105	62-63	0.3860879164639499
1105	64-65	0.3905069678692499
1105	66-67	0.37958577298097396
1105	68-69	0.3419016338645946
1105	70-71	0.3307158500796312
1105	72-73	0.3575817054966848
1105	74-75	0.4059190696171413
1105	76-77	0.33826502591821495
1105	78-79	0.4459342594592748
1105	80-81	0.4817865644523671
1105	82-83	0.40926546941664554
1105	84-85	0.41521596514162695
1105	86-87	0.43677362112869744
1105	88-89	0.3994015211516313
1105	90-91	0.3683365738198603
1105	92-93	0.4317275242947858
1105	94-95	0.5141571527170861
1105	96-97	0.5007189876112363
1105	98-99	0.5008560138997566
1105	100	0.4845465448132025
1106	1	-0.3811906177868778
1106	2	2.004816184511135
1106	3	2.7757332900017637
1106	4	1.2415028230986636
1106	5	2.4305491870354814
1106	6	1.0990411707110042
1106	7	0.5922930566586047
1106	8	0.3947172804876615
1106	9	0.3321963883474197
1106	10-11	0.3923572400863691
1106	12-13	0.34717145485371503
1106	14-15	0.45053946856980076
1106	16-17	0.3237556108909061
1106	18-19	0.2254979929042591
1106	20-21	0.1393321406052621
1106	22-23	0.11937655586113749
1106	24-25	0.09864026599569797
1106	26-27	0.0974016875729049
1106	28-29	0.206815944432293
1106	30-31	0.2630210584964985
1106	32-33	0.20632719452510884
1106	34-35	0.2605010418607847
1106	36-37	0.22507391922484032
1106	38-39	0.2323988150763796
1106	40-41	0.19836695600109522
1106	42-43	0.19684753256428422
1106	44-45	0.2893105813614767
1106	46-47	0.28055196754961287
1106	48-49	0.27227981448277916
1106	50-51	0.25988403886236
1106	52-53	0.2578290838110604
1106	54-55	0.29174586779890177
1106	56-57	0.10094758897821521
1106	58-59	0.06379207567753298
1106	60-61	0.09220237479541993
1106	62-63	0.18126827174481264
1106	64-65	0.1670852484940255
1106	66-67	0.17938697315359775
1106	68-69	0.11836670919263526
1106	70-71	0.1365173932775896
1106	72-73	0.17350336303950797
1106	74-75	0.1632964837099813
1106	76-77	0.16834231525986
1106	78-79	0.27370624053000725
1106	80-81	0.21650910803611723
1106	82-83	0.2287421431156389
1106	84-85	0.16884252196005178
1106	86-87	0.1506931456271552
1106	88-89	0.13787567324453676
1106	90-91	0.2530424399923632
1106	92-93	0.24423279738478954
1106	94-95	0.2918554665533719
1106	96-97	0.394059256960098
1106	98-99	0.3492195703698435
1106	100	0.36710856039681516
1107	1	-1.0098757060181747
1107	2	1.7412018481179032
1107	3	-0.5310061292261636
1107	4	0.003962886958412071
1107	5	1.8550399900936512
1107	6	0.7493684525407645
1107	7	0.29791192019865065
1107	8	0.2818243822737472
1107	9	0.27510296603524154
1107	10-11	0.2711232297114847
1107	12-13	0.2641405103085219
1107	14-15	0.337573878074096
1107	16-17	0.24957914543948334
1107	18-19	0.20347687145389415
1107	20-21	0.14880176772359732
1107	22-23	0.0815068513630024
1107	24-25	-0.04960112805905226
1107	26-27	0.028216897606007763
1107	28-29	0.04246434649889608
1107	30-31	0.08413534670581413
1107	32-33	0.12988549004696637
1107	34-35	0.11630204061228255
1107	36-37	0.2096390635255716
1107	38-39	0.17746563843794405
1107	40-41	0.18368959908770677
1107	42-43	0.14789552684026575
1107	44-45	0.3222329276827338
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2216	90-91	-0.15327496227703108
2216	92-93	-0.17500912577934002
2216	94-95	-0.19960347622055608
2216	96-97	-0.27425323378901467
2216	98-99	-0.34131885822958097
2216	100	-0.3091715150222498
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	797.0
3	194.0
4	329.0
5	946.0
6	2928.0
7	5294.0
8	6361.0
9	8767.0
10	5912.0
11	3862.0
12	3533.0
13	4945.0
14	4984.0
15	5545.0
16	6684.0
17	7953.0
18	11113.0
19	19223.0
20	37992.0
21	44434.0
22	19783.0
23	12476.0
24	12704.0
25	13611.0
26	14074.0
27	15501.0
28	18382.0
29	21874.0
30	24789.0
31	30777.0
32	39935.0
33	56928.0
34	83774.0
35	187124.0
36	231305.0
37	356450.0
38	344562.0
39	89254.0
40	700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.061287575776067	43.72674817507216	24.35556478230047	18.856399466851304
2	14.31788667896931	43.235196956234475	23.899596746633858	18.54731961816235
3	18.11026581969188	25.95442716713589	18.234202236209367	37.701104776962865
4	33.60635243555783	27.84965705072164	20.35147531124007	18.192515202480468
5	16.51598168314576	41.6694764660318	21.12065430354946	20.693887547272976
6	37.73329543454399	24.117187006031717	22.67581781922847	15.473699740195823
7	18.59210104394074	23.68302794644571	40.13963255609708	17.58523845351647
8	36.90655934990281	24.19810012421695	21.98081898896172	16.91452153691852
9	36.02246042969611	26.21780739139275	22.042671171358453	15.717061007552688
10-11	15.904155316183685	45.4518996764436	22.09387293192444	16.55007207544827
12-13	15.774100566180982	25.825222590968554	31.566284067823254	26.83439277502721
14-15	16.07664089112706	35.17583732534305	22.01923454791807	26.72828723561182
16-17	26.25303351921262	25.1755183822294	31.746743220607826	16.824704877950154
18-19	16.449946719413784	25.476691808116986	32.02875158261282	26.044609889856414
20-21	26.315711536457194	35.60202506095516	22.05226794183161	16.029995460756044
22-23	25.689928061241634	25.854724828980995	32.12004904889455	16.33529806088282
24-25	16.083475386419515	35.600259483004606	21.827071321945166	26.489193808630713
26-27	16.794595274966298	29.930994300593976	36.7558151654206	16.518595259019122
28-29	25.822374884596698	25.649576061952423	32.16589712148144	16.362151931969436
30-31	23.113949831387306	27.09666653187523	32.53470357370063	17.25468006303683
32-33	18.777861247215654	28.79352932767361	33.690302819400166	18.738306605710562
34-35	18.617136699588052	28.671391201384665	33.83337158752226	18.878100511505018
36-37	19.000210160730244	28.457015865711284	34.115721674291876	18.427052299266602
38-39	19.70439099236302	28.17067899002107	33.583570784582975	18.54135923303294
40-41	18.17693824862641	29.569443882813466	33.75799279985921	18.495625068700917
42-43	18.241320254268324	28.699645685144464	34.3479518824365	18.71108217815072
44-45	18.374455162578403	29.25505709936046	33.408038163821715	18.96244957423942
46-47	19.135931846412944	28.323429959807473	33.94428975070609	18.596348443073495
48-49	18.46071218858195	28.679621072799332	33.844335257053906	19.015331481564804
50-51	18.81656157680919	29.39695830787009	32.96778275873263	18.818697356588082
52-53	19.179015305895796	28.975779618793553	32.969841564557115	18.87536351075354
54-55	19.416487878168287	30.00784827876084	30.865833731537606	19.709830111533268
56-57	19.861726769408115	30.680248707283692	29.600056726310925	19.857967796997265
58-59	20.850934531800053	31.02823842592461	28.30719803348789	19.813629008787455
60-61	20.365315164207292	31.600712837859003	27.92124838891012	20.112723609023583
62-63	19.41273443393419	31.570222892888903	27.695632499970813	21.321410173206093
64-65	19.976403905002794	31.462143445804447	28.694713916570176	19.866738732622583
66-67	20.160314523562924	30.806439349390036	29.079874484458212	19.953371642588827
68-69	20.379781512576326	30.92694551027766	28.905273325705277	19.787999651440742
70-71	18.7129050648736	31.890922594214942	29.414927335076506	19.98124500583495
72-73	18.80892463598326	31.165517090155635	29.614336326380435	20.411221947480666
74-75	19.178974736479397	31.618428715238277	28.567470244917892	20.635126303364434
76-77	20.201379321342916	30.854298717785888	28.696040701883085	20.24828125898811
78-79	19.774489297567907	31.247314246985336	28.206009537003947	20.772186918442813
80-81	20.202069677844126	32.00035539749986	27.756630171026504	20.04094475362951
82-83	20.03442348634053	32.18997504240286	27.600129401325223	20.17547206993139
84-85	20.112987238957643	32.406230074665096	26.88185577467709	20.598926911700172
86-87	19.977121520493384	32.52018953199926	27.174416654302867	20.32827229320449
88-89	21.20103155315614	31.65829346069795	27.05574499131165	20.084929994834262
90-91	20.278733737802906	32.47396126261713	26.952111504885956	20.295193494694004
92-93	19.639861965976742	32.600542545017966	27.06818946815666	20.69140602084863
94-95	19.87588553014483	32.818315996966625	26.787470202303965	20.51832827058458
96-97	20.024638699331835	31.91326676631879	27.130306312114683	20.93178822223469
98-99	20.838707233884776	32.03369659904356	26.208611473326325	20.918984693745337
100	19.886046181823776	32.99415251973603	25.95707139598553	21.162729902454664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87016.0
1	46895.0
2	4874.5
3	2510.0
4	1991.5
5	1829.5
6	1820.0
7	2011.0
8	1994.5
9	1792.5
10	1725.5
11	2671.0
12	18269.0
13	20323.0
14	6633.0
15	5672.5
16	6025.0
17	6536.5
18	7123.0
19	7796.0
20	8606.5
21	9625.5
22	10751.5
23	12070.0
24	13466.5
25	15118.5
26	16981.5
27	18842.0
28	20615.5
29	22434.0
30	24390.5
31	26208.0
32	28320.5
33	30604.5
34	33156.5
35	35860.0
36	38380.0
37	40800.0
38	42196.5
39	43223.5
40	44091.5
41	44194.5
42	44386.5
43	45106.0
44	46748.0
45	47795.0
46	48934.0
47	50540.0
48	51406.0
49	55501.0
50	70089.5
51	72206.5
52	56784.5
53	49628.5
54	48804.5
55	49414.5
56	45353.0
57	37853.5
58	32000.0
59	26226.0
60	21885.0
61	18618.5
62	15552.5
63	12184.0
64	9251.0
65	7018.0
66	5226.0
67	3986.5
68	3035.0
69	2232.0
70	1668.0
71	1297.0
72	1005.0
73	710.0
74	479.5
75	308.0
76	208.5
77	154.0
78	108.0
79	77.5
80	42.5
81	20.5
82	8.5
83	2.5
84	1.5
85	2.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13908197920149173
2	0.004499376067533926
3	0.0034172476462282983
4	0.0
5	0.002221210970048394
6	0.014010715349536023
7	0.0027337981169826384
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.1390825487427663E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.11266038994213E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	2.847706371856915E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.8477063718569157E-5
64-65	0.0
66-67	2.8477063718569157E-5
68-69	0.0
70-71	0.0
72-73	4.271559557785373E-4
74-75	3.7020182834139896E-4
76-77	4.556330194971065E-4
78-79	3.7020182834139896E-4
80-81	0.0010536513575870586
82-83	0.0011960366761799043
84-85	0.001452330249647027
86-87	2.8477063718569157E-5
88-89	0.0
90-91	8.543119115570745E-5
92-93	0.0
94-95	2.8477063718569157E-5
96-97	0.0013953761222098885
98-99	7.688807204013671E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1755799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.02484952428789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.17413649417895	55.31012397509758
2	7.470796167796902	9.26750016268459
3	1.55812558995256	2.8992751577024944
4	0.5471046855313948	1.3573634317647043
5	0.2734093293236542	0.8479086254918061
6	0.15491216497180266	0.5765042233114631
7	0.10479498285153249	0.4549925129586642
8	0.07469235267317396	0.37062255481349254
9	0.052892005031528994	0.2952556787806617
>10	0.519022093858894	6.780824875829487
>50	0.04340151159732702	1.8862643865688182
>100	0.021755801930557213	2.690554315671712
>500	0.003029167962148718	1.291444143345191
>1k	0.0014686874967993783	2.139027309680326
>5k	9.179296854996114E-5	0.3671133375783535
>10k+	3.671718741998446E-4	13.465225308720678
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	133899	7.626100709705382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79910	4.5512043235017225	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	12146	0.6917648318514819	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10549	0.600809090334372	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6448	0.3672402137146678	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	4886	0.27827786665785775	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4554	0.25936909634872785	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4189	0.23858083983417233	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3744	0.21323625312464584	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3108	0.17701342807462586	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	2690	0.15320660280590204	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1967	0.11202876866885104	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.10787111736593996	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1756	0.10001144777961486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011960366761799043	0.0	0.0	0.0	0.0
2	0.0011960366761799043	0.0	0.0	0.0	0.0
3	0.0011960366761799043	0.0	0.0	0.0	0.0
4	0.0011960366761799043	0.0	0.0	0.0	0.0
5	0.0011960366761799043	0.0	0.0	0.0	0.0
6	0.0011960366761799043	0.0	0.0	0.0	0.0
7	0.0011960366761799043	0.0	0.0	0.0	0.0
8	0.0011960366761799043	0.0	0.0	0.0	0.0
9	0.0012529908036170428	5.6954127437138306E-5	0.0	0.0	0.0
10-11	0.00133842199477275	8.543119115570746E-5	0.0	0.0	0.0
12-13	0.0014238531859284576	1.1390825487427661E-4	0.0	0.0	0.0
14-15	0.0015377614408027343	1.1390825487427661E-4	0.0	0.0	0.0
16-17	0.0015377614408027343	1.1390825487427661E-4	0.0	0.0	0.0
18-19	0.0015947155682398725	1.1390825487427661E-4	0.0	0.0	0.0
20-21	0.0015947155682398725	1.1390825487427661E-4	0.0	0.0	0.0
22-23	0.0016231926319584416	1.1390825487427661E-4	0.0	0.0	0.0
24-25	0.001651669695677011	1.1390825487427661E-4	0.0	0.0	0.0
26-27	0.001651669695677011	1.1390825487427661E-4	0.0	0.0	0.0
28-29	0.0017086238231141491	1.1390825487427661E-4	0.0	0.0	0.0
30-31	0.0017655779505512876	1.1390825487427661E-4	0.0	0.0	0.0
32-33	0.0017940550142698567	1.1390825487427661E-4	0.0	0.0	0.0
34-35	0.0020788256514555482	1.1390825487427661E-4	0.0	0.0	0.0
36-37	0.0025344586709526544	1.4238531859284576E-4	0.0	0.0	0.0
38-39	0.004186128366629666	1.7086238231141493E-4	0.0	0.0	0.0
40-41	0.006948403547330873	1.7086238231141493E-4	0.0	5.6954127437138306E-5	0.0
42-43	0.009710678728032081	1.7086238231141493E-4	0.0	5.6954127437138306E-5	0.0
44-45	0.0118179814432062	1.7086238231141493E-4	0.0	5.6954127437138306E-5	0.0
46-47	0.014323963050440284	1.7086238231141493E-4	0.0	5.6954127437138306E-5	0.0
48-49	0.017655779505512875	2.2781650974855323E-4	0.0	5.6954127437138306E-5	0.0
50-51	0.021927339063298246	3.132477009042607E-4	0.0	5.6954127437138306E-5	0.0
52-53	0.02759427474329351	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
54-55	0.03531155901102575	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
56-57	0.04445269646468645	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
58-59	0.0561282925892998	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
60-61	0.07002509968396155	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
62-63	0.08907625531168431	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
64-65	0.1086684751500599	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
66-67	0.13290245637456222	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
68-69	0.1637715934454912	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
70-71	0.2065156660870635	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
72-73	0.2531326193943612	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
74-75	0.30359397630366575	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
76-77	0.3618580486718582	3.4172476462282985E-4	0.0	5.6954127437138306E-5	0.0
78-79	0.4307725428707956	3.986788920599682E-4	0.0	5.6954127437138306E-5	0.0
80-81	0.5132705964634904	4.5563301949710645E-4	0.0	5.6954127437138306E-5	0.0
82-83	0.6162436588698365	4.5563301949710645E-4	0.0	5.6954127437138306E-5	0.0
84-85	0.7408023355748579	4.5563301949710645E-4	0.0	5.6954127437138306E-5	0.0
86-87	0.8831591771039852	4.5563301949710645E-4	0.0	5.6954127437138306E-5	0.0
88	1.0021648263838856	4.5563301949710645E-4	0.0	5.6954127437138306E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2585	0.0	87.06017	1
CGTGTGG	1470	0.0	71.62708	3
AACGTGT	1440	0.0	69.96864	1
GTGGAAT	46340	0.0	68.88297	6
ATCGAGT	920	0.0	68.57552	1
AACGAGT	39150	0.0	68.10311	1
TGGAATC	46915	0.0	68.030975	7
GAATCAC	47140	0.0	67.686325	9
GGAATCA	47170	0.0	67.62335	8
ACGAGTG	42915	0.0	67.61547	2
CGAGTGG	44495	0.0	67.166985	3
ACGTGTG	1530	0.0	66.97674	2
GAGTGGA	46385	0.0	66.32332	4
AGTGGAA	46595	0.0	66.02441	5
TCGAGTG	1025	0.0	63.28694	2
ATGAGTG	1670	0.0	55.732388	2
TGAGTGG	1835	0.0	55.074295	3
GCGAGTG	520	0.0	52.43045	2
AATGAGT	1700	0.0	50.68207	1
GTGTGGA	2470	0.0	46.24273	4
>>END_MODULE