FastQCFastQC Report
Thu 26 May 2016
SRR2132763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112141
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1265295.990556501672947No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG282291.3365111514808907No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101370.47993954949030393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100090.47387934801701215No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42930.2032534759753255No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34990.16566128871131236No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29020.13739613027728736No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27200.1287792813074506No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA23250.1100778783234642No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG22740.1076632667989495No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT22540.10671636031874766No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT21900.10368625958210177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT29300.088.3750461
ATCGAGT9350.066.9691241
AACGTGT13400.065.700921
ACGTGTG15400.060.44232
TCGAGTG11250.058.920052
GTGGAAT596800.055.147796
TGGAATC597250.055.085287
CGTGTGG17700.054.978593
GGAATCA607100.054.2289628
AGTGGAA591600.054.0155335
GAATCAC609950.053.990999
CGAGTGG594050.052.46713
GAGTGGA611350.052.2628444
ACGAGTG580450.052.1656952
AACGAGT540750.051.036851
ATGAGTG18000.044.1378062
TGAGTGG20400.043.784463
AGTACTT204750.040.8123220-21
AGCGAGT6700.040.755651
GTACTTT195000.040.0450822-23
Produced by FastQC (version 0.11.5)