##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132763_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2112141
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.815560608879807	31.0	25.0	33.0	16.0	34.0
2	28.09292277362165	31.0	25.0	33.0	16.0	34.0
3	29.14805308925872	31.0	28.0	34.0	16.0	34.0
4	34.69590903258826	35.0	35.0	37.0	32.0	37.0
5	33.76858599875671	35.0	35.0	37.0	28.0	37.0
6	35.21922163340421	37.0	35.0	37.0	32.0	37.0
7	35.63786177153893	37.0	35.0	37.0	33.0	37.0
8	35.91212802554375	37.0	35.0	37.0	35.0	37.0
9	37.87386069395935	39.0	38.0	39.0	35.0	39.0
10-11	37.86737272748363	39.0	38.0	39.0	35.0	39.0
12-13	37.85673020882602	39.0	38.0	39.0	35.0	39.0
14-15	39.284928184245274	41.0	39.0	41.0	36.0	41.0
16-17	39.303268342407065	41.0	39.0	41.0	36.0	41.0
18-19	39.29234269871188	41.0	39.0	41.0	36.0	41.0
20-21	39.28465263445953	40.5	39.0	41.0	36.0	41.0
22-23	39.1935386889417	40.0	39.0	41.0	36.0	41.0
24-25	39.0815501900678	40.0	39.0	41.0	36.0	41.0
26-27	38.953149434625814	40.0	39.0	41.0	35.5	41.0
28-29	38.792905397887736	40.0	39.0	41.0	35.0	41.0
30-31	38.65982337353425	40.0	38.5	41.0	35.0	41.0
32-33	38.504317893549725	40.0	38.0	41.0	34.5	41.0
34-35	38.395008666561566	40.0	38.0	41.0	34.0	41.0
36-37	38.21491983726465	40.0	38.0	41.0	34.0	41.0
38-39	37.79903709080028	40.0	36.5	41.0	33.5	41.0
40-41	37.58494579670581	40.0	36.0	41.0	33.0	41.0
42-43	37.445363259365735	40.0	35.5	41.0	33.0	41.0
44-45	37.292669618174166	40.0	35.0	41.0	33.0	41.0
46-47	37.14178788253247	40.0	35.0	41.0	33.0	41.0
48-49	36.97714830591329	40.0	35.0	41.0	32.5	41.0
50-51	36.69578451438611	39.0	35.0	41.0	31.5	41.0
52-53	36.31876020587641	39.0	35.0	41.0	31.0	41.0
54-55	35.56539478188246	39.0	35.0	41.0	28.5	41.0
56-57	34.980607592012085	39.0	35.0	41.0	25.0	41.0
58-59	34.652017786691324	38.5	35.0	40.5	23.0	41.0
60-61	34.384471254523255	38.0	35.0	40.0	23.0	41.0
62-63	34.10437039951405	37.5	34.5	40.0	22.5	41.0
64-65	33.79012977826764	37.0	34.0	40.0	22.0	41.0
66-67	33.423389347586166	36.0	34.0	39.5	20.5	41.0
68-69	33.0947640332724	36.0	34.0	39.0	20.0	41.0
70-71	32.757928566322036	35.0	34.0	39.0	19.0	40.5
72-73	32.428071089950905	35.0	33.0	37.5	17.5	40.0
74-75	32.08089587768998	35.0	33.0	37.0	14.0	39.5
76-77	31.733787422335915	35.0	33.0	37.0	9.0	39.0
78-79	31.383668514554664	35.0	33.0	36.0	6.0	39.0
80-81	31.06965420395703	35.0	33.0	36.0	2.0	37.5
82-83	30.758976081615764	35.0	33.0	35.0	2.0	37.0
84-85	30.487495389749075	35.0	32.0	35.0	2.0	37.0
86-87	30.303624379243622	35.0	32.0	35.0	2.0	36.0
88-89	30.086129666532678	35.0	32.0	35.0	2.0	36.0
90-91	29.88529790388047	35.0	32.0	35.0	2.0	36.0
92-93	29.733180218555482	35.0	32.0	35.0	2.0	35.5
94-95	29.540502030877676	35.0	32.0	35.0	2.0	35.0
96-97	29.322583814243462	35.0	31.5	35.0	2.0	35.0
98-99	29.15346087216715	34.5	31.0	35.0	2.0	35.0
100	29.06320269338079	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.0200449377628793
1101	2	2.168244205495519
1101	3	1.0647207421058518
1101	4	0.5733669641407246
1101	5	2.2255227639661044
1101	6	0.9543806739215768
1101	7	0.54899947188553
1101	8	0.3689881431005517
1101	9	0.26705959470707086
1101	10-11	0.4553276419679051
1101	12-13	0.4112465331715853
1101	14-15	0.5106212537365167
1101	16-17	0.45456427472507954
1101	18-19	0.39544717412471186
1101	20-21	0.45387898580849395
1101	22-23	0.4844737091476148
1101	24-25	0.4552408783201969
1101	26-27	0.5220868993536811
1101	28-29	0.5535576347468165
1101	30-31	0.6112333360303666
1101	32-33	0.5560306517247682
1101	34-35	0.5111790171581205
1101	36-37	0.5870306259328686
1101	38-39	0.5679206732864657
1101	40-41	0.5187271700442224
1101	42-43	0.41166574823296287
1101	44-45	0.5820956895836886
1101	46-47	0.5097161050234433
1101	48-49	0.537588058812112
1101	50-51	0.48380573772562485
1101	52-53	0.4917921992554781
1101	54-55	0.41358546861612666
1101	56-57	0.44656868731814114
1101	58-59	0.4083794931194902
1101	60-61	0.42586596064933957
1101	62-63	0.4453764396710156
1101	64-65	0.5294040334870758
1101	66-67	0.5854046900866194
1101	68-69	0.6480666028808599
1101	70-71	0.555185924771628
1101	72-73	0.5310675819600235
1101	74-75	0.469584929894026
1101	76-77	0.4342227029895973
1101	78-79	0.473137069179586
1101	80-81	0.5529713293364402
1101	82-83	0.4519086014662683
1101	84-85	0.46984598907764763
1101	86-87	0.5573117045631051
1101	88-89	0.570272293107994
1101	90-91	0.5631092804242748
1101	92-93	0.6072585401893491
1101	94-95	0.6273566703705598
1101	96-97	0.6507513582357518
1101	98-99	0.6579020983396262
1101	100	0.6556625948487813
1102	1	1.5025892617606758
1102	2	3.183601451507446
1102	3	3.2967851681410743
1102	4	1.455764741401616
1102	5	2.6885478294222267
1102	6	1.1997797453946362
1102	7	0.7185474007626453
1102	8	0.5332404522766794
1102	9	0.4495508381135309
1102	10-11	0.5436614992910265
1102	12-13	0.5005767379024917
1102	14-15	0.67870972195675
1102	16-17	0.5716585255773055
1102	18-19	0.498789547346874
1102	20-21	0.4244092464316438
1102	22-23	0.39948039321244266
1102	24-25	0.4510238255625225
1102	26-27	0.5068129804979691
1102	28-29	0.5930421821602252
1102	30-31	0.6237586515531603
1102	32-33	0.6348129420628226
1102	34-35	0.7013593633662722
1102	36-37	0.6180314805048326
1102	38-39	0.6569343115051751
1102	40-41	0.6300054324770485
1102	42-43	0.7461599958475347
1102	44-45	0.754525477082403
1102	46-47	0.7420562860086619
1102	48-49	0.7356372831794289
1102	50-51	0.8163857185893519
1102	52-53	0.9075037986853047
1102	54-55	0.8362905850568865
1102	56-57	0.6561216175286617
1102	58-59	0.654391845646181
1102	60-61	0.6371036245239523
1102	62-63	0.653782641665245
1102	64-65	0.7082143551486482
1102	66-67	0.7338103553108297
1102	68-69	0.6805959739994023
1102	70-71	0.5894648253643808
1102	72-73	0.5814289645092074
1102	74-75	0.7075200437702094
1102	76-77	0.6522109315377627
1102	78-79	0.675071741711534
1102	80-81	0.5461595047188048
1102	82-83	0.6393285013203176
1102	84-85	0.613622524048214
1102	86-87	0.6107275215569032
1102	88-89	0.6734529963108074
1102	90-91	0.7008768453713223
1102	92-93	0.728131652015584
1102	94-95	0.7346564019003452
1102	96-97	0.8079079510922789
1102	98-99	0.8200829012283002
1102	100	0.8661590048797372
1103	1	1.1735478158562884
1103	2	2.971441022367557
1103	3	3.225463786301514
1103	4	1.4197568562493004
1103	5	2.6220235742735483
1103	6	1.212222961957039
1103	7	0.6801584992529541
1103	8	0.42695586211488035
1103	9	0.37914971640908846
1103	10-11	0.45624948868303505
1103	12-13	0.45484659999375054
1103	14-15	0.550066482758325
1103	16-17	0.4234235245879958
1103	18-19	0.45903051512637205
1103	20-21	0.4131708280544544
1103	22-23	0.4365152453576897
1103	24-25	0.36220838160743796
1103	26-27	0.4730209013142357
1103	28-29	0.4880134840815984
1103	30-31	0.5894366614723054
1103	32-33	0.5955701821292294
1103	34-35	0.6649299433776861
1103	36-37	0.6243468491422135
1103	38-39	0.6465234107136482
1103	40-41	0.7039429440291585
1103	42-43	0.6549481055850137
1103	44-45	0.7956902225370825
1103	46-47	0.7413882383818233
1103	48-49	0.7663885629696097
1103	50-51	0.8004453180946101
1103	52-53	0.8901001992321582
1103	54-55	0.9510336377116886
1103	56-57	0.8682145050137962
1103	58-59	0.942915219781348
1103	60-61	1.0178743240665895
1103	62-63	0.9828102861103432
1103	64-65	0.9007055877614576
1103	66-67	0.9011171002753287
1103	68-69	0.8900338108425387
1103	70-71	0.9823894400838142
1103	72-73	0.961804103643388
1103	74-75	0.9445977252227564
1103	76-77	0.9322670900420214
1103	78-79	0.9309998145311198
1103	80-81	0.9236516859274886
1103	82-83	0.9789438192302136
1103	84-85	0.8968948704021464
1103	86-87	0.9749583324394564
1103	88-89	0.9900625029513854
1103	90-91	0.9971628278927298
1103	92-93	0.9884930466876511
1103	94-95	0.9749036556542237
1103	96-97	0.9850666173909097
1103	98-99	0.9898978476794547
1103	100	1.0269197869135738
1104	1	6.199531339288328
1104	2	5.216310781521994
1104	3	4.2584950357543825
1104	4	1.920296243650661
1104	5	2.8930546324829436
1104	6	1.336061191527321
1104	7	0.7616457270171964
1104	8	0.5825324275167816
1104	9	0.4603568128887261
1104	10-11	0.4879144247309526
1104	12-13	0.4405403760961306
1104	14-15	0.601174280778281
1104	16-17	0.5681066193383586
1104	18-19	0.5763496317970151
1104	20-21	0.5712214375420146
1104	22-23	0.553062654210656
1104	24-25	0.5064631987207591
1104	26-27	0.5829145187653353
1104	28-29	0.6267000185209142
1104	30-31	0.7022790647293675
1104	32-33	0.7522221553712996
1104	34-35	0.7626445838736302
1104	36-37	0.7084461664568877
1104	38-39	0.7727434295955717
1104	40-41	0.8302356893022775
1104	42-43	0.8811009198943367
1104	44-45	0.8657681609863133
1104	46-47	0.8393661753495962
1104	48-49	0.9589411111542105
1104	50-51	1.0016462462988542
1104	52-53	0.9483351835892506
1104	54-55	1.0618679845007506
1104	56-57	1.1170862358614997
1104	58-59	1.1576789465509947
1104	60-61	1.081052682847421
1104	62-63	1.188282543012285
1104	64-65	1.1836624273418508
1104	66-67	1.1557409499525733
1104	68-69	1.1283588681819055
1104	70-71	1.1248422588375249
1104	72-73	1.07789023862626
1104	74-75	1.048519697885716
1104	76-77	1.028274007129891
1104	78-79	1.0832195182653521
1104	80-81	1.1362868189696478
1104	82-83	1.0915343736881375
1104	84-85	1.1099313453809003
1104	86-87	1.098962216567461
1104	88-89	1.1076832994669843
1104	90-91	1.1602430060454516
1104	92-93	1.1328502194626608
1104	94-95	1.1464712730164344
1104	96-97	1.2154079331190708
1104	98-99	1.2019565567321386
1104	100	1.2665182294913748
1105	1	1.590458832794333
1105	2	3.094762298465845
1105	3	3.264931707676933
1105	4	1.4344800772827995
1105	5	2.6384229869272318
1105	6	1.2331493392580128
1105	7	0.7534169469639806
1105	8	0.4798307742397441
1105	9	0.31445868216334816
1105	10-11	0.4193090821849168
1105	12-13	0.4113195207085951
1105	14-15	0.5217103716023246
1105	16-17	0.40058866104162405
1105	18-19	0.35628574439695626
1105	20-21	0.37083251095274505
1105	22-23	0.3540448142628847
1105	24-25	0.3904634001127576
1105	26-27	0.39757535680697487
1105	28-29	0.49385628179231134
1105	30-31	0.5044303738669242
1105	32-33	0.5339502591947962
1105	34-35	0.5212963533524331
1105	36-37	0.6285630283675303
1105	38-39	0.47985634682634526
1105	40-41	0.4513804141531992
1105	42-43	0.5335729325259138
1105	44-45	0.605119890771256
1105	46-47	0.6324525457803816
1105	48-49	0.5318747058087183
1105	50-51	0.5873438376771531
1105	52-53	0.7037772682332744
1105	54-55	0.6982817527607637
1105	56-57	0.6747306790831828
1105	58-59	0.6866650524521631
1105	60-61	0.6610891641022576
1105	62-63	0.6223442414435212
1105	64-65	0.6733051973889062
1105	66-67	0.6476368318526937
1105	68-69	0.6808853907041765
1105	70-71	0.6749009890750628
1105	72-73	0.6917505590967252
1105	74-75	0.6490434105844543
1105	76-77	0.6059578885770343
1105	78-79	0.7323355562925897
1105	80-81	0.6609721849033328
1105	82-83	0.6870832989649323
1105	84-85	0.7462773075143758
1105	86-87	0.807452338578738
1105	88-89	0.7089426696696499
1105	90-91	0.6749867468190445
1105	92-93	0.8036517089216524
1105	94-95	0.8816483666416524
1105	96-97	0.8665504001039146
1105	98-99	0.8612448776568193
1105	100	0.9274258698311577
1106	1	-0.7046798260423905
1106	2	1.8954693419135218
1106	3	2.776438804775019
1106	4	1.1952525785392183
1106	5	2.456820121693724
1106	6	1.0815971671974864
1106	7	0.5581219154982335
1106	8	0.42169387627840393
1106	9	0.2824424232882734
1106	10-11	0.3802199648688216
1106	12-13	0.32950426233824004
1106	14-15	0.34849469210857364
1106	16-17	0.28084374826932645
1106	18-19	0.2897201839737278
1106	20-21	0.3139242935522333
1106	22-23	0.32853178222291746
1106	24-25	0.3407279623475006
1106	26-27	0.27540217048437654
1106	28-29	0.32376509225607464
1106	30-31	0.32926555776232647
1106	32-33	0.2758085671707704
1106	34-35	0.4158215876285851
1106	36-37	0.3428290816096293
1106	38-39	0.4417284660121297
1106	40-41	0.26812736790341773
1106	42-43	0.30339516975875824
1106	44-45	0.39815427928316893
1106	46-47	0.3643863360040456
1106	48-49	0.3753011570765352
1106	50-51	0.30357707259753397
1106	52-53	0.3175355278915575
1106	54-55	0.30233607541626384
1106	56-57	0.4145832912817582
1106	58-59	0.48857815279404804
1106	60-61	0.5047421213656165
1106	62-63	0.5365792679428338
1106	64-65	0.526772786023848
1106	66-67	0.5126489543673927
1106	68-69	0.47375333366883865
1106	70-71	0.5695501148588775
1106	72-73	0.559178296681587
1106	74-75	0.45880396061965456
1106	76-77	0.49402514335130476
1106	78-79	0.48806809715119925
1106	80-81	0.4943165607406108
1106	82-83	0.456774911455998
1106	84-85	0.4866823142395269
1106	86-87	0.473077777922267
1106	88-89	0.5003775110858264
1106	90-91	0.5068996370840289
1106	92-93	0.4960756893618772
1106	94-95	0.4968838978642012
1106	96-97	0.5189784312577821
1106	98-99	0.5355481091244023
1106	100	0.4886193867501589
1107	1	-1.1993840312057529
1107	2	1.5844789326790298
1107	3	-0.7090694195051555
1107	4	-0.13823927588185114
1107	5	1.8395257426805074
1107	6	0.744002301235291
1107	7	0.2910019775963164
1107	8	0.2437323823609745
1107	9	0.24274807176056612
1107	10-11	0.304460547785375
1107	12-13	0.25655587797094626
1107	14-15	0.3511765668921001
1107	16-17	0.21242979409901608
1107	18-19	0.21344888919441019
1107	20-21	0.23002117518516485
1107	22-23	0.247839794529078
1107	24-25	0.15380015935469515
1107	26-27	0.2575426206475129
1107	28-29	0.24200188872386974
1107	30-31	0.3125235656140717
1107	32-33	0.2987615657481584
1107	34-35	0.24479343375606533
1107	36-37	0.3277099730670372
1107	38-39	0.32277804500041896
1107	40-41	0.34662247224649434
1107	42-43	0.32785619606295313
1107	44-45	0.35821755188870696
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2216	96-97	-0.26721894316225203
2216	98-99	-0.44766550238473357
2216	100	-0.493527778120022
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	974.0
3	238.0
4	220.0
5	328.0
6	669.0
7	1013.0
8	1446.0
9	1997.0
10	2256.0
11	3961.0
12	3604.0
13	5193.0
14	5244.0
15	5746.0
16	6663.0
17	7717.0
18	10611.0
19	18306.0
20	35352.0
21	42274.0
22	20367.0
23	14221.0
24	14988.0
25	15920.0
26	16915.0
27	18574.0
28	21634.0
29	25605.0
30	30214.0
31	37159.0
32	48686.0
33	65976.0
34	98355.0
35	168273.0
36	295235.0
37	487188.0
38	474434.0
39	104428.0
40	157.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.82428093810388	38.91216701844614	24.20475082445351	22.05880121899647
2	16.19374770658491	38.105183414022086	24.89705377540512	20.80401510398788
3	19.64531411423696	23.32763275390068	18.437113234150825	38.58993989771153
4	33.15242685029077	24.894644817746542	21.061378004593443	20.89155032736924
5	18.99017178310312	36.84989503248454	21.084740081114276	23.075193103298073
6	37.42111324085368	21.375565370890882	22.83923200472752	18.364089383527915
7	20.578147711508507	21.249994081691597	38.20784152190485	19.964016684895057
8	36.407891329224704	21.33214591260716	22.434913199450225	19.825049558717907
9	35.95465454247609	22.765951704928792	22.751700762401754	18.527692990193362
10-11	18.34967457191542	39.77004849581539	22.53287067482711	19.347406257442092
12-13	18.294635632753682	22.73093983782333	30.773561045403692	28.200863484019294
14-15	18.633628152665942	31.055242050601734	22.191723942672386	28.11940585405993
16-17	27.439503328613	22.345525227719172	30.839773481031806	19.37519796263602
18-19	18.93746676950071	22.56980002755498	30.927172949154436	27.565560253789872
20-21	27.53897585435821	31.20561079965779	22.40889694390668	18.846516402077324
22-23	26.9689381532767	22.792109996444367	31.030101683552374	19.20885016672656
24-25	18.653607879398205	31.17452859444516	22.246313101256025	27.925550424900607
26-27	18.956428571766754	26.69317531357992	35.219831441177455	19.130564673475874
28-29	27.653433175152607	22.48602247671912	30.974636636474557	18.885907711653722
30-31	25.276579546535956	24.146351971767036	31.08622956516634	19.490838916530667
32-33	20.855118100543475	25.521567925626176	31.804529148385452	21.818784825444894
34-35	21.296589574275583	25.228950150581802	32.35046807954583	21.12399219559679
36-37	21.626042011399807	24.46983889806599	33.16279547624898	20.74132361428522
38-39	22.903868633770188	24.350741735518604	32.13599376178011	20.6093958689311
40-41	20.500856713637962	26.635934816851716	31.93806663475592	20.925141834754406
42-43	20.60590221308134	24.731348505745167	32.948672970276824	21.714076310896665
44-45	20.68394108158499	25.68102697689217	31.635814086275488	21.999217855247352
46-47	21.725348828510977	24.51519571846766	32.70612615350964	21.053329299511727
48-49	20.764144060458083	24.857833828328697	32.55578107711559	21.82224103409763
50-51	21.88291406681656	25.787695991886906	31.394187225189985	20.93520271610655
52-53	21.81730894195199	25.190097529069455	31.787625769983467	21.204967758995085
54-55	21.330630862238838	26.660720093970998	29.590614452349534	22.418034591440627
56-57	21.757709357471875	27.24789680234416	29.256238101528258	21.738155738655703
58-59	23.8008968151274	26.817362098458393	27.800227352245898	21.581513734168315
60-61	22.708024701002444	28.02928402980672	27.34786645399147	21.914824815199363
62-63	21.418253524861044	28.20099519918187	27.13125076936188	23.249500506595204
64-65	22.14480472657839	28.005421986505635	27.746230010212386	22.10354327670359
66-67	22.398457299962455	27.025231743524696	28.742162573426675	21.834148383086166
68-69	23.115525904757305	26.831044897097307	28.40968003556581	21.64374916257958
70-71	21.066491299586534	28.61906473100044	28.504110284303934	21.81033368510909
72-73	20.993825902329423	27.02138906663201	29.220969064174774	22.763815966863792
74-75	21.15179299788413	27.89883496919938	28.04449341021612	22.90487862270037
76-77	22.21333702629221	27.010537952846125	28.590177439572184	22.18594758128948
78-79	21.613538342373555	27.367788367644202	28.196689178648427	22.82198411133382
80-81	22.46220982395438	28.295321816011665	27.272109626191277	21.97035873384268
82-83	22.215201904623473	27.923438189102107	27.682756240342954	22.17860366593147
84-85	21.508266482838163	28.70174827252418	26.667790343653408	23.12219490098425
86-87	21.66068024357281	28.724580585429806	27.219046270832838	22.395692900164548
88-89	23.372658359456118	27.737210725988465	26.680060658829124	22.2100702557263
90-91	22.500520798810687	28.7574924010719	26.407529803895574	22.334456996221842
92-93	21.56792562617742	28.87423235475283	26.243181681525996	23.314660337543753
94-95	22.07651432279245	29.05455389923161	26.070448438444316	22.79848333953163
96-97	22.365283140359306	28.2237236135343	26.71561444334234	22.695378802764054
98-99	23.238092938452148	28.23378685676869	25.898844246729247	22.62927595804991
100	21.791869008745156	29.677658830542093	25.665900145870946	22.864572014841812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9965.0
1	5742.5
2	1286.0
3	1003.0
4	959.5
5	944.0
6	956.0
7	1027.5
8	1038.5
9	1026.5
10	1043.5
11	1585.5
12	11509.5
13	13520.5
14	5675.0
15	5571.5
16	6310.0
17	7064.5
18	7861.0
19	8786.5
20	9666.5
21	10683.0
22	11801.0
23	13025.0
24	14598.0
25	16198.0
26	17828.0
27	19536.0
28	21236.0
29	22949.0
30	24899.5
31	27132.5
32	29261.5
33	31605.0
34	34554.0
35	37103.0
36	39566.0
37	42286.5
38	44648.0
39	46644.0
40	48452.0
41	50213.5
42	51801.0
43	53569.0
44	55923.5
45	58069.5
46	60498.5
47	64396.0
48	69796.5
49	85113.5
50	121839.5
51	125035.0
52	88666.0
53	69043.0
54	63168.5
55	60954.0
56	57305.5
57	53097.5
58	48842.0
59	42685.0
60	36185.5
61	30284.5
62	24950.0
63	20252.5
64	16360.0
65	12854.0
66	9797.0
67	7654.0
68	6072.0
69	4645.0
70	3289.0
71	2331.0
72	1732.0
73	1269.0
74	914.5
75	665.5
76	497.5
77	319.5
78	198.0
79	125.0
80	67.5
81	39.5
82	22.5
83	12.5
84	6.5
85	4.0
86	3.0
87	3.5
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13564435328891394
2	0.005492057585170687
3	0.0035508993007569096
4	0.0
5	0.0027933741165954355
6	0.011978366974553309
7	0.0024146115245146987
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.522158321816584E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	7.101798601513819E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	4.734532401009213E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.971259021059674E-4
74-75	3.78762592080737E-4
76-77	6.391618741362438E-4
78-79	3.0774460606559884E-4
80-81	8.522158321816584E-4
82-83	8.285431701766122E-4
84-85	0.0013019964102775337
86-87	2.3672662005046064E-5
88-89	0.0
90-91	4.734532401009213E-5
92-93	0.0
94-95	2.3672662005046064E-5
96-97	0.0012309784242623954
98-99	5.681438881211055E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2112141.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.03314585831629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.11834530683306	64.72422712548702
2	5.559681631272699	7.898433524799908
3	1.3433236780549334	2.8626152027461793
4	0.6529558433393847	1.8552603063586575
5	0.37696824874236484	1.3388620298435228
6	0.23578808514514638	1.004926166626097
7	0.1726916586560564	0.8586782244481155
8	0.12162277185205315	0.6911398474127692
9	0.09512728169209916	0.6081471067986018
>10	0.292824855359763	3.330082437879233
>50	0.012223591004682394	0.6058824993739808
>100	0.013569642824446103	1.822555929996375
>500	0.002004413105490051	0.9762980724851542
>1k	0.0026057370371370316	3.1219611835178305
>5k	0.0	0.0
>10k+	2.6725508073200326E-4	8.300930342226517
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	126529	5.990556501672947	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	28229	1.3365111514808907	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10137	0.47993954949030393	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10009	0.47387934801701215	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4293	0.2032534759753255	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3499	0.16566128871131236	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2902	0.13739613027728736	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2720	0.1287792813074506	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	2325	0.1100778783234642	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	2274	0.1076632667989495	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	2254	0.10671636031874766	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	2190	0.10368625958210177	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.995611561917505E-4	0.0	0.0	0.0	0.0
2	8.995611561917505E-4	0.0	0.0	0.0	0.0
3	9.942518042119347E-4	0.0	0.0	0.0	0.0
4	9.942518042119347E-4	0.0	0.0	0.0	0.0
5	9.942518042119347E-4	0.0	0.0	0.0	0.0
6	9.942518042119347E-4	0.0	0.0	0.0	0.0
7	9.942518042119347E-4	0.0	0.0	4.734532401009213E-5	0.0
8	9.942518042119347E-4	0.0	0.0	9.469064802018426E-5	0.0
9	0.0010415971282220268	0.0	0.0	9.469064802018426E-5	0.0
10-11	0.0010415971282220268	0.0	0.0	9.469064802018426E-5	0.0
12-13	0.0010415971282220268	0.0	0.0	1.4203597203027638E-4	0.0
14-15	0.0010652697902270728	0.0	0.0	2.3672662005046064E-4	2.3672662005046064E-5
16-17	0.001112615114237165	0.0	0.0	2.3672662005046064E-4	4.734532401009213E-5
18-19	0.001136287776242211	0.0	0.0	2.3672662005046064E-4	4.734532401009213E-5
20-21	0.0011599604382472573	0.0	0.0	2.8407194406055277E-4	7.101798601513819E-5
22-23	0.0012073057622573494	0.0	0.0	2.8407194406055277E-4	9.469064802018426E-5
24-25	0.0012309784242623954	0.0	0.0	2.8407194406055277E-4	9.469064802018426E-5
26-27	0.0013019964102775334	0.0	0.0	3.314172680706449E-4	9.469064802018426E-5
28-29	0.00144403238230781	0.0	0.0	3.314172680706449E-4	1.4203597203027638E-4
30-31	0.0017281043263683627	0.0	0.0	3.78762592080737E-4	1.4203597203027638E-4
32-33	0.00222523022847433	0.0	0.0	3.78762592080737E-4	1.4203597203027638E-4
34-35	0.002935410088625712	0.0	0.0	3.78762592080737E-4	1.6570863403532243E-4
36-37	0.0048528957110344426	0.0	0.0	4.497805780958752E-4	1.893812960403685E-4
38-39	0.007598924503619787	0.0	0.0	4.734532401009213E-4	1.893812960403685E-4
40-41	0.013067309426785429	0.0	0.0	4.734532401009213E-4	1.893812960403685E-4
42-43	0.019458928168147864	0.0	0.0	4.734532401009213E-4	1.893812960403685E-4
44-45	0.02544811165542452	0.0	0.0	4.734532401009213E-4	1.893812960403685E-4
46-47	0.030845478592575023	0.0	0.0	4.734532401009213E-4	1.893812960403685E-4
48-49	0.03692935272787186	0.0	0.0	5.207985641110134E-4	1.893812960403685E-4
50-51	0.04530947507765817	0.0	0.0	5.207985641110134E-4	1.893812960403685E-4
52-53	0.05539402909180779	0.0	0.0	5.681438881211055E-4	1.893812960403685E-4
54-55	0.07085227738110288	0.0	0.0	5.681438881211055E-4	1.893812960403685E-4
56-57	0.08687866955851906	0.0	0.0	5.681438881211055E-4	2.3672662005046064E-4
58-59	0.10321280634200083	0.0	0.0	5.681438881211055E-4	2.3672662005046064E-4
60-61	0.12679077769902672	0.0	0.0	6.154892121311977E-4	2.3672662005046064E-4
62-63	0.1556714253451829	0.0	0.0	6.154892121311977E-4	2.3672662005046064E-4
64-65	0.18857642553219695	0.0	0.0	6.628345361412898E-4	2.3672662005046064E-4
66-67	0.222002224283322	0.0	0.0	6.628345361412898E-4	2.3672662005046064E-4
68-69	0.2648497425124554	0.0	0.0	6.628345361412898E-4	2.3672662005046064E-4
70-71	0.328363494671994	0.0	0.0	6.628345361412898E-4	2.3672662005046064E-4
72-73	0.3965644339085317	0.0	0.0	6.628345361412898E-4	2.3672662005046064E-4
74-75	0.46637511416141253	0.0	0.0	6.628345361412898E-4	2.3672662005046064E-4
76-77	0.5382689886707375	0.0	0.0	6.628345361412898E-4	2.3672662005046064E-4
78-79	0.6185429855298487	0.0	0.0	6.865071981463359E-4	2.8407194406055277E-4
80-81	0.7231998242541573	0.0	0.0	7.33852522156428E-4	2.8407194406055277E-4
82-83	0.8495644940370932	0.0	0.0	7.57525184161474E-4	2.8407194406055277E-4
84-85	1.0055436639883417	0.0	0.0	8.048705081715662E-4	3.0774460606559884E-4
86-87	1.1813841973618238	0.0	0.0	8.522158321816584E-4	3.314172680706449E-4
88	1.3184252377090355	0.0	0.0	8.522158321816584E-4	3.314172680706449E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2930	0.0	88.375046	1
ATCGAGT	935	0.0	66.969124	1
AACGTGT	1340	0.0	65.70092	1
ACGTGTG	1540	0.0	60.4423	2
TCGAGTG	1125	0.0	58.92005	2
GTGGAAT	59680	0.0	55.14779	6
TGGAATC	59725	0.0	55.08528	7
CGTGTGG	1770	0.0	54.97859	3
GGAATCA	60710	0.0	54.228962	8
AGTGGAA	59160	0.0	54.015533	5
GAATCAC	60995	0.0	53.99099	9
CGAGTGG	59405	0.0	52.4671	3
GAGTGGA	61135	0.0	52.262844	4
ACGAGTG	58045	0.0	52.165695	2
AACGAGT	54075	0.0	51.03685	1
ATGAGTG	1800	0.0	44.137806	2
TGAGTGG	2040	0.0	43.78446	3
AGTACTT	20475	0.0	40.81232	20-21
AGCGAGT	670	0.0	40.75565	1
GTACTTT	19500	0.0	40.04508	22-23
>>END_MODULE