FastQCFastQC Report
Thu 26 May 2016
SRR2132762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266047
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1398166.17003972115318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA478942.1135483950685927No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107620.47492395347492794No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG51890.22898907215958011No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46920.20705660562203698No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40680.17951966574391442No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39560.17457713807348213No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38920.17175283654752085No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33010.14567217714372208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT25900.085.6253361
AACGAGT326900.079.5213851
ACGAGTG362800.077.926752
CGAGTGG379100.077.159343
GTGGAAT405300.076.886616
GGAATCA410500.075.987768
GAATCAC410700.075.927879
TGGAATC411250.075.73497
GAGTGGA398050.075.513794
AGTGGAA403950.074.45745
AACGTGT13200.070.983961
ATCGAGT10200.066.472711
TCGAGTG11100.066.068142
CGTGTGG16500.063.5277443
ACGTGTG18950.056.5608672
ATGAGTG19450.045.6806872
TGAGTGG21800.043.986173
AATGAGT20000.042.3763581
AGTACTT238150.041.01894420-21
GAGTACT235050.040.54017320-21
Produced by FastQC (version 0.11.5)