##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132762_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2266047 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.79910919764683 31.0 25.0 33.0 16.0 34.0 2 28.065076320129283 31.0 25.0 33.0 16.0 34.0 3 29.147851302289848 31.0 28.0 34.0 16.0 34.0 4 34.69884516958386 35.0 35.0 37.0 32.0 37.0 5 33.786073722213175 35.0 35.0 37.0 28.0 37.0 6 35.22626406248414 37.0 35.0 37.0 32.0 37.0 7 35.649783521701 37.0 35.0 37.0 33.0 37.0 8 35.91416065068377 37.0 35.0 37.0 35.0 37.0 9 37.87859916409501 39.0 38.0 39.0 35.0 39.0 10-11 37.8709303028578 39.0 38.0 39.0 35.0 39.0 12-13 37.845691197049305 39.0 38.0 39.0 35.0 39.0 14-15 39.2546449830917 41.0 39.0 41.0 36.0 41.0 16-17 39.2456440665176 40.5 39.0 41.0 36.0 41.0 18-19 39.12733319300085 40.0 39.0 41.0 35.5 41.0 20-21 39.074217348536905 40.0 39.0 41.0 35.0 41.0 22-23 38.956587175817624 40.0 39.0 41.0 35.0 41.0 24-25 38.840539715195675 40.0 39.0 41.0 35.0 41.0 26-27 38.66712693955597 40.0 39.0 41.0 35.0 41.0 28-29 38.511822129020274 40.0 38.0 41.0 35.0 41.0 30-31 38.39815568697384 40.0 38.0 41.0 34.5 41.0 32-33 38.24714293216337 40.0 38.0 41.0 34.0 41.0 34-35 38.14531362323906 40.0 38.0 41.0 34.0 41.0 36-37 37.991417433089424 40.0 38.0 41.0 34.0 41.0 38-39 37.55338547700026 40.0 36.0 41.0 33.0 41.0 40-41 37.317577923141044 40.0 35.0 41.0 33.0 41.0 42-43 37.16591910935651 40.0 35.0 41.0 33.0 41.0 44-45 37.01831272696462 40.0 35.0 41.0 33.0 41.0 46-47 36.87918057304195 40.0 35.0 41.0 33.0 41.0 48-49 36.71521618924939 39.0 35.0 41.0 32.0 41.0 50-51 36.46273731303896 39.0 35.0 41.0 31.5 41.0 52-53 36.10679147431629 39.0 35.0 41.0 30.5 41.0 54-55 35.383322146451505 39.0 35.0 41.0 27.5 41.0 56-57 34.75181847508017 39.0 35.0 41.0 23.5 41.0 58-59 34.42552250681473 38.5 35.0 40.5 22.0 41.0 60-61 34.179078368630485 38.0 35.0 40.0 22.0 41.0 62-63 33.90261058133393 37.0 34.0 40.0 20.5 41.0 64-65 33.62231079055289 37.0 34.0 40.0 20.0 41.0 66-67 33.314319164606914 36.0 34.0 39.5 18.5 41.0 68-69 32.985778979871114 36.0 34.0 39.0 17.0 41.0 70-71 32.644387119949414 35.0 34.0 39.0 12.5 40.5 72-73 32.302663845895516 35.0 33.0 38.5 8.5 40.0 74-75 31.955269462636917 35.0 33.0 37.0 4.5 39.5 76-77 31.614695105617844 35.0 33.0 37.0 2.0 39.0 78-79 31.277254178752692 35.0 33.0 36.0 2.0 39.0 80-81 30.968660182246882 35.0 33.0 36.0 2.0 38.0 82-83 30.68610867294456 35.0 33.0 35.5 2.0 37.0 84-85 30.41866850069747 35.0 33.0 35.0 2.0 37.0 86-87 30.204701182279095 35.0 33.0 35.0 2.0 36.0 88-89 29.997967385495535 35.0 32.0 35.0 2.0 36.0 90-91 29.806712967559807 35.0 32.0 35.0 2.0 36.0 92-93 29.629230549940047 35.0 32.0 35.0 2.0 35.5 94-95 29.4510074151154 35.0 32.0 35.0 2.0 35.0 96-97 29.278531733896074 35.0 32.0 35.0 2.0 35.0 98-99 29.11135845814319 35.0 32.0 35.0 2.0 35.0 100 29.003389161831155 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3248682863514958 1101 2 2.401105101861827 1101 3 1.1156749330450815 1101 4 0.6904266133296986 1101 5 2.2655640338003096 1101 6 0.9774456283754205 1101 7 0.5735483222945135 1101 8 0.3733181076357752 1101 9 0.3366184766417817 1101 10-11 0.4637060842632579 1101 12-13 0.36265812561913435 1101 14-15 0.5066277747796377 1101 16-17 0.43564086784959954 1101 18-19 0.37094321857695434 1101 20-21 0.44910268861667646 1101 22-23 0.41194628508285547 1101 24-25 0.3470308533301676 1101 26-27 0.29469703505258593 1101 28-29 0.3260802761974375 1101 30-31 0.359244790823567 1101 32-33 0.4115453270718348 1101 34-35 0.3872819735186397 1101 36-37 0.45081345762258707 1101 38-39 0.49454858454515005 1101 40-41 0.5473371677344971 1101 42-43 0.5982828812334589 1101 44-45 0.6640926092708312 1101 46-47 0.5977137507924084 1101 48-49 0.6195630006020849 1101 50-51 0.6476142126250863 1101 52-53 0.6933170918091136 1101 54-55 0.9130405955366783 1101 56-57 0.7935798026479617 1101 58-59 0.7862539657902943 1101 60-61 0.8332193069516123 1101 62-63 0.7393103735321489 1101 64-65 0.7805356570651156 1101 66-67 0.7440743728238459 1101 68-69 0.8391705162543346 1101 70-71 0.7175042461387875 1101 72-73 0.8243643845341921 1101 74-75 0.8004497346440296 1101 76-77 0.7388801352688468 1101 78-79 0.8373638308788216 1101 80-81 0.8571565938243033 1101 82-83 0.8162994282045943 1101 84-85 0.8385716599731374 1101 86-87 0.8298959884841359 1101 88-89 0.8244469578966012 1101 90-91 0.785360217587268 1101 92-93 0.8527781157203052 1101 94-95 0.8024984909872863 1101 96-97 0.8409619734769294 1101 98-99 0.8382769660724811 1101 100 0.8041698699580202 1102 1 1.4667576719352873 1102 2 3.0551132579184106 1102 3 3.3081925337135836 1102 4 1.4850494582820701 1102 5 2.6736154221117516 1102 6 1.240723986585209 1102 7 0.7610689655150367 1102 8 0.5476938054709493 1102 9 0.4626658971217239 1102 10-11 0.560975333120858 1102 12-13 0.4947801534342915 1102 14-15 0.6171884000100718 1102 16-17 0.578862626000145 1102 18-19 0.5766697908329874 1102 20-21 0.47654845031742354 1102 22-23 0.47152154556751924 1102 24-25 0.4976020649233206 1102 26-27 0.5649406894918982 1102 28-29 0.6050056445815741 1102 30-31 0.6327277826028848 1102 32-33 0.6474824424336774 1102 34-35 0.6717860263324198 1102 36-37 0.6727819532944679 1102 38-39 0.6764097074541624 1102 40-41 0.720033840052956 1102 42-43 0.773540638994028 1102 44-45 0.8501598502308596 1102 46-47 0.7900517120173305 1102 48-49 0.7817580683432226 1102 50-51 0.8461150880358446 1102 52-53 0.8045179790613659 1102 54-55 0.8510575538886442 1102 56-57 0.7275353556135826 1102 58-59 0.6242323365886762 1102 60-61 0.673960068445048 1102 62-63 0.662871418257474 1102 64-65 0.715663068833905 1102 66-67 0.7575821084516647 1102 68-69 0.7223259471443271 1102 70-71 0.6752746699929872 1102 72-73 0.6929368953288062 1102 74-75 0.7454652723912822 1102 76-77 0.6931379711010344 1102 78-79 0.6937779003565936 1102 80-81 0.6628686724009647 1102 82-83 0.7469807917609828 1102 84-85 0.7514956141028826 1102 86-87 0.7231985638391265 1102 88-89 0.7353257716860675 1102 90-91 0.7010466371217596 1102 92-93 0.7430617099585426 1102 94-95 0.6974592852110355 1102 96-97 0.7025183854545354 1102 98-99 0.7151781875385197 1102 100 0.7369455701839023 1103 1 1.374028625024767 1103 2 3.0090617007791955 1103 3 3.215120217228506 1103 4 1.370281730068946 1103 5 2.589734783240168 1103 6 1.1876818801178501 1103 7 0.661672771389135 1103 8 0.412017761290592 1103 9 0.34733829379388936 1103 10-11 0.43216295906317015 1103 12-13 0.40795522127594097 1103 14-15 0.5465527595767057 1103 16-17 0.3977148389807752 1103 18-19 0.3730014110460118 1103 20-21 0.3423765236147318 1103 22-23 0.2530622349541005 1103 24-25 0.3735591727229419 1103 26-27 0.47170846504265285 1103 28-29 0.4427635046909657 1103 30-31 0.5001116144337843 1103 32-33 0.5170869831169682 1103 34-35 0.5067194793008625 1103 36-37 0.4385255360863809 1103 38-39 0.4478695247954434 1103 40-41 0.5060921177018116 1103 42-43 0.5015064583528925 1103 44-45 0.6078423000538677 1103 46-47 0.6024464619867587 1103 48-49 0.6592793263480345 1103 50-51 0.6610335077191252 1103 52-53 0.5844677294449951 1103 54-55 0.6144317446212995 1103 56-57 0.6411344539033692 1103 58-59 0.5930357953918417 1103 60-61 0.6101520211188856 1103 62-63 0.6297061335269802 1103 64-65 0.6137637179691495 1103 66-67 0.6324522078857981 1103 68-69 0.6106565747015011 1103 70-71 0.5808458792436255 1103 72-73 0.54923694277403 1103 74-75 0.5975023506694406 1103 76-77 0.597186773333366 1103 78-79 0.5675980725723413 1103 80-81 0.4848188825334425 1103 82-83 0.4747540688772922 1103 84-85 0.41550029192186244 1103 86-87 0.4732476407731845 1103 88-89 0.43712162526789555 1103 90-91 0.46939217603383554 1103 92-93 0.46830600980803183 1103 94-95 0.5171003870446889 1103 96-97 0.5881054743298684 1103 98-99 0.6039720425397554 1103 100 0.6364403843818955 1104 1 6.18069651577018 1104 2 5.212978381819518 1104 3 4.16602847747259 1104 4 1.9183908066128197 1104 5 2.8692989122926917 1104 6 1.3157365902553835 1104 7 0.7067850187915283 1104 8 0.5456618127933908 1104 9 0.4296594438344101 1104 10-11 0.47737412191943207 1104 12-13 0.41615129396149086 1104 14-15 0.5573271253895413 1104 16-17 0.4360897075314085 1104 18-19 0.41245724004097895 1104 20-21 0.3579968193348435 1104 22-23 0.34475964268916215 1104 24-25 0.29554640892006745 1104 26-27 0.41246861794121514 1104 28-29 0.3040277802001796 1104 30-31 0.43854323572607257 1104 32-33 0.5042187007900054 1104 34-35 0.48906028878784724 1104 36-37 0.45942018927334516 1104 38-39 0.551217009220899 1104 40-41 0.6095236802630524 1104 42-43 0.6345882373226033 1104 44-45 0.6373234328583735 1104 46-47 0.5314084741249232 1104 48-49 0.6673180616397687 1104 50-51 0.6823776079996122 1104 52-53 0.7414579918367821 1104 54-55 0.7544354415863879 1104 56-57 0.7103845449364954 1104 58-59 0.8739899956820381 1104 60-61 0.8890905099256798 1104 62-63 0.8998222911809108 1104 64-65 0.9690773015956751 1104 66-67 0.9751676720187419 1104 68-69 0.9223394789581434 1104 70-71 0.8991239713870769 1104 72-73 0.9329392616613745 1104 74-75 0.8864674662799708 1104 76-77 0.8371306264488574 1104 78-79 0.8555146843718404 1104 80-81 0.9328499395130727 1104 82-83 0.8991743161288319 1104 84-85 0.8805546985126007 1104 86-87 0.8877092655955217 1104 88-89 0.8789448101847697 1104 90-91 0.910999910727746 1104 92-93 0.9325100526062755 1104 94-95 0.931727077030672 1104 96-97 0.961414251901779 1104 98-99 1.0164936880230613 1104 100 1.08495890908263 1105 1 1.7734927926859783 1105 2 3.2457887355159833 1105 3 3.3188485930640006 1105 4 1.4256493469024605 1105 5 2.6014603242642806 1105 6 1.187568730520141 1105 7 0.7325114569054207 1105 8 0.5011795062947257 1105 9 0.3832401781900501 1105 10-11 0.4656018733900211 1105 12-13 0.41168281058374134 1105 14-15 0.5000753204335879 1105 16-17 0.44293221246675785 1105 18-19 0.3495512259153344 1105 20-21 0.3324218427883636 1105 22-23 0.28429897523562175 1105 24-25 0.34285985853369283 1105 26-27 0.373037945218222 1105 28-29 0.45749189115149136 1105 30-31 0.48076706935519553 1105 32-33 0.481315462235969 1105 34-35 0.5073380419339202 1105 36-37 0.5241430079297231 1105 38-39 0.49880836978366716 1105 40-41 0.4838341429664581 1105 42-43 0.49679005195100245 1105 44-45 0.6111548925139871 1105 46-47 0.53300172431927 1105 48-49 0.6063415971252297 1105 50-51 0.685417453005499 1105 52-53 0.809660922034027 1105 54-55 0.816395738763461 1105 56-57 0.8668475481957572 1105 58-59 0.8645540201353725 1105 60-61 0.8749240805643765 1105 62-63 0.9623583959738866 1105 64-65 0.9190962860248391 1105 66-67 0.9036371932653537 1105 68-69 0.886840598999413 1105 70-71 0.9659884125499616 1105 72-73 0.9688270086809041 1105 74-75 0.9622038572011427 1105 76-77 0.8544130654914177 1105 78-79 0.9033415613304427 1105 80-81 0.8647005114957658 1105 82-83 0.7775857893459026 1105 84-85 0.7868734161050668 1105 86-87 0.8576245859631051 1105 88-89 0.7943111369073286 1105 90-91 0.6732611885400388 1105 92-93 0.6688858876975345 1105 94-95 0.7450935837181447 1105 96-97 0.7951103520434408 1105 98-99 0.8046230528897702 1105 100 0.7702606887460313 1106 1 -0.6367693997516604 1106 2 1.9305029964672684 1106 3 2.8255207394274393 1106 4 1.231420200864406 1106 5 2.4733420393752255 1106 6 1.1318339331067264 1106 7 0.645269166873824 1106 8 0.4201258678991806 1106 9 0.33369174096847587 1106 10-11 0.45224652781462993 1106 12-13 0.34331056358546164 1106 14-15 0.4266822495991178 1106 16-17 0.332570598308763 1106 18-19 0.3547071101688246 1106 20-21 0.302074494892409 1106 22-23 0.22029219903073027 1106 24-25 0.24798092631434798 1106 26-27 0.1864312209347645 1106 28-29 0.3012121613478058 1106 30-31 0.3110136476224312 1106 32-33 0.2083367249511241 1106 34-35 0.2831669314380534 1106 36-37 0.36510660335125067 1106 38-39 0.477268389878283 1106 40-41 0.5144785024687195 1106 42-43 0.49414309691641733 1106 44-45 0.5674941863879397 1106 46-47 0.5291680033724262 1106 48-49 0.5490176831552134 1106 50-51 0.61556001318489 1106 52-53 0.6911363074751549 1106 54-55 0.6953019434821641 1106 56-57 0.6806378503186323 1106 58-59 0.6983868433752463 1106 60-61 0.7539081943860708 1106 62-63 0.7732572998817702 1106 64-65 0.758647880047782 1106 66-67 0.7754436276945995 1106 68-69 0.7938512995046878 1106 70-71 0.7076646740776198 1106 72-73 0.7295930223039448 1106 74-75 0.6225083739611641 1106 76-77 0.6491243313903361 1106 78-79 0.7226744626142221 1106 80-81 0.685098395171817 1106 82-83 0.7278898909501557 1106 84-85 0.7112802218561036 1106 86-87 0.7601115038385551 1106 88-89 0.8118666323162209 1106 90-91 0.8193053740542808 1106 92-93 0.7654966799684146 1106 94-95 0.8408766254674482 1106 96-97 0.8060366993654569 1106 98-99 0.7493757265333905 1106 100 0.7370627842582813 1107 1 -1.0718373125249485 1107 2 1.657952903776831 1107 3 -0.8483689680188498 1107 4 -0.16383637107530546 1107 5 1.8401635157784852 1107 6 0.7024463760195516 1107 7 0.2794140271198131 1107 8 0.20421914286244203 1107 9 0.1512249171444111 1107 10-11 0.22396689101888256 1107 12-13 0.24784131042424207 1107 14-15 0.24676432495397904 1107 16-17 0.18294730653090596 1107 18-19 0.18185768366587496 1107 20-21 0.15577416269133693 1107 22-23 0.19571767042209132 1107 24-25 0.07392990503259256 1107 26-27 0.05260933980597571 1107 28-29 0.0646097849806253 1107 30-31 0.04925706717772016 1107 32-33 0.10419954169734069 1107 34-35 0.07708284834097867 1107 36-37 0.14617459372387742 1107 38-39 0.1502896033154002 1107 40-41 0.17438862641256492 1107 42-43 0.15896306575578478 1107 44-45 0.2907273359804776 1107 46-47 0.29084758194056093 1107 48-49 0.20594437975854163 1107 50-51 0.18170596066504174 1107 52-53 0.2754105913225615 1107 54-55 0.32312628867099846 1107 56-57 0.2473780934322889 1107 58-59 0.22546581321033443 1107 60-61 0.18560538603520627 1107 62-63 0.12546669494012974 1107 64-65 0.06640385628607959 1107 66-67 0.09461573838105153 1107 68-69 0.08318657122818252 1107 70-71 0.03204581586467725 1107 72-73 0.08027341524336151 1107 74-75 0.12187172905277421 1107 76-77 0.07121874588296961 1107 78-79 0.10922792469028764 1107 80-81 0.16445096226888367 1107 82-83 0.18472687737996907 1107 84-85 0.24310188874324723 1107 86-87 0.2433443188542519 1107 88-89 0.19691397142517175 1107 90-91 0.14892524415130737 1107 92-93 0.2048487227076521 1107 94-95 0.17699681053061767 1107 96-97 0.23443205335830442 1107 98-99 0.28803007111914525 1107 100 0.2809024724664013 1108 1 -1.531480708339835 1108 2 0.1086475577659769 1108 3 1.8863550305188674 1108 4 0.8222761247732322 1108 5 0.7900407161629417 1108 6 0.44867862469447317 1108 7 0.1920813703453419 1108 8 0.13136339317953372 1108 9 0.10449687011131914 1108 10-11 0.2721716367176299 1108 12-13 0.2477437001912577 1108 14-15 0.3060833584065179 1108 16-17 0.12822578474738577 1108 18-19 0.1829444916263796 1108 20-21 0.19088956322379147 1108 22-23 0.18024518297221448 1108 24-25 0.1498212926113851 1108 26-27 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-0.559350405876188 2215 66-67 -0.6828788605927514 2215 68-69 -0.6235390898441437 2215 70-71 -0.6529371177779666 2215 72-73 -0.6544764331294672 2215 74-75 -0.6428031800816001 2215 76-77 -0.7961550715357824 2215 78-79 -0.7160942553763832 2215 80-81 -0.6874214672632242 2215 82-83 -0.6899421990888222 2215 84-85 -0.6138929382246978 2215 86-87 -0.561019832055738 2215 88-89 -0.557314987814788 2215 90-91 -0.6337296590507933 2215 92-93 -0.7431503520345792 2215 94-95 -0.7211343569321862 2215 96-97 -0.7294264486095301 2215 98-99 -0.6583608702088881 2215 100 -0.7741431706320192 2216 1 2.930439265276231 2216 2 -1.9050663603861544 2216 3 -2.244629285412117 2216 4 -0.8747728456535242 2216 5 -2.442458003539233 2216 6 -1.169135928528931 2216 7 -0.6046011983428556 2216 8 -0.4106001867766693 2216 9 -0.4899915508560184 2216 10-11 -0.24038105576139657 2216 12-13 -0.28056914342791117 2216 14-15 -0.39458188836845665 2216 16-17 -0.4509543917750136 2216 18-19 -0.39451327933106484 2216 20-21 -0.3292055532871032 2216 22-23 -0.39449530803263855 2216 24-25 -0.4280736041257711 2216 26-27 -0.35643164613777145 2216 28-29 -0.3513031770296422 2216 30-31 -0.35741455797295885 2216 32-33 -0.26519293337571526 2216 34-35 -0.36647567358824773 2216 36-37 -0.3807385085923798 2216 38-39 -0.2925696516748957 2216 40-41 -0.2758827072637331 2216 42-43 -0.2744541997257741 2216 44-45 -0.2588678133548754 2216 46-47 -0.32106756461885055 2216 48-49 -0.24660579471873945 2216 50-51 -0.29597030061789553 2216 52-53 -0.311637959075199 2216 54-55 -0.08539194431840968 2216 56-57 0.04275237096960183 2216 58-59 0.15044963276174883 2216 60-61 0.16755796261134748 2216 62-63 0.19234060209980441 2216 64-65 0.20040437225003416 2216 66-67 0.1505404981636218 2216 68-69 0.10143917330078978 2216 70-71 0.14245003288937141 2216 72-73 0.19310960272233757 2216 74-75 0.1020576571165357 2216 76-77 0.18351709374224257 2216 78-79 0.14227601947075286 2216 80-81 0.09721799247389029 2216 82-83 0.08985890833177379 2216 84-85 0.18190509375443398 2216 86-87 0.20228746777321405 2216 88-89 0.15596046400177954 2216 90-91 0.2075889880280073 2216 92-93 0.1536981381748035 2216 94-95 0.24127279391717948 2216 96-97 0.17412235429201672 2216 98-99 0.1248059300407185 2216 100 0.0468494549070293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1029.0 3 237.0 4 308.0 5 637.0 6 1932.0 7 3346.0 8 4112.0 9 5729.0 10 4142.0 11 4252.0 12 3916.0 13 5394.0 14 5737.0 15 6115.0 16 7288.0 17 8520.0 18 11941.0 19 19927.0 20 38467.0 21 45452.0 22 22077.0 23 15466.0 24 15456.0 25 16560.0 26 17519.0 27 19661.0 28 23075.0 29 27370.0 30 32405.0 31 39746.0 32 51227.0 33 70984.0 34 105498.0 35 201761.0 36 301122.0 37 483675.0 38 515179.0 39 128479.0 40 306.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.733536363347133 38.967931745150786 25.11307758386035 21.18545430764173 2 16.043890185389827 38.19613862399864 25.384257575704083 20.375713614907447 3 19.924411861794802 25.78770006937445 19.140021642003138 35.14786642682761 4 30.463666464111295 27.30940708643731 21.87395054030212 20.352975909149283 5 18.380438447375898 36.634486556904776 21.96927973394481 23.015795261774514 6 34.76043947895951 23.829296302279044 23.78961891168316 17.620645307078288 7 20.311810456524118 23.194786212837467 36.6812857238684 19.812117606770016 8 33.608084916155754 23.486759100760047 23.492760741502714 19.412395241581486 9 33.194236483179736 25.307683379912245 23.333364224131273 18.164715912776742 10-11 18.12442548632045 39.848092294643486 23.381178766371573 18.646303452664487 12-13 18.046051118975026 25.164438336892392 30.513665427063074 26.275845117069508 14-15 18.258999041061372 32.225964421744116 23.341197247894684 26.173839289299828 16-17 25.77139838670601 24.852507472263376 30.664765558702005 18.71132858232861 18-19 18.44725638965123 24.936861415495795 30.854920484879617 25.76096170997336 20-21 25.837791537421772 32.533018953269725 23.34636042412183 18.282829085186673 22-23 25.39830374215539 25.286611442745894 30.938281509606817 18.376803305491897 24-25 18.253416632576464 32.468060018172615 23.323986660470855 25.954536688780067 26-27 18.6114409807034 27.874532169897627 35.02202293244579 18.492003916953177 28-29 24.469858745206963 25.144977134190068 31.970276874221938 18.41488724638103 30-31 23.155080190304965 25.853943011773367 32.191587376607814 18.799389421313855 32-33 20.3409284979526 27.03092654300639 32.842677137764575 19.785467821276434 34-35 19.921718305048394 26.937901111495037 33.169987206796684 19.970393376659885 36-37 20.05871899391319 26.91151154411184 33.20774017485074 19.82202928712423 38-39 20.410852025575814 26.79898078018682 32.96924114989672 19.820926044340652 40-41 19.940561691791917 27.43535328261064 32.814500317071975 19.809584708525463 42-43 19.974095607947838 27.243322558224204 32.935735483942146 19.846846349885812 44-45 19.974872542361215 27.341908618841533 32.65128657966935 20.0319322591279 46-47 20.125994738855812 27.078520436689974 32.82921757580491 19.9662672486493 48-49 19.88623801712851 27.347777870450173 32.66249552635051 20.10348858607081 50-51 20.16959489366284 27.651986035594145 32.19761108220615 19.980807988536867 52-53 20.36662587603148 27.605676503049587 31.90120399303985 20.126493627879082 54-55 20.66854747496411 28.227922898333528 30.557552424993833 20.545977201708528 56-57 20.866447165482445 28.90279857390425 29.484560558540927 20.746193702072375 58-59 21.29781509386169 29.478911955489007 28.539897892673892 20.683375057975407 60-61 21.129371985664903 29.6201049669314 28.340519856825562 20.91000319057813 62-63 20.731061785360538 29.81606952902335 28.08539012769597 21.36747855792015 64-65 20.799634782508925 29.636388830417022 28.687114609714627 20.87686177735943 66-67 20.827524760077793 29.388004750122125 28.90983726286348 20.8746332269366 68-69 20.954265291055304 29.36249777696579 28.83847951962161 20.84475741235729 70-71 20.390795071770356 29.821049607532412 28.964580169784654 20.82357515091258 72-73 20.31144639988703 29.523596229545817 29.082055038746006 21.082902331821153 74-75 20.447591491675233 29.710448560142044 28.68546642721806 21.156493520964663 76-77 20.71793258222679 29.551911058723952 28.569776850264194 21.16037950878506 78-79 20.787104549589714 29.89252835177851 28.07850436852491 21.241862730106867 80-81 20.963426083752292 30.15245843221232 27.782826995700628 21.101288488334763 82-83 20.82603805833263 30.267298876025666 27.695412422168104 21.211250643473594 84-85 20.83239280827653 30.397174078004113 27.303072808444227 21.467360305275125 86-87 20.937032638775808 30.44680891437821 27.241469395824534 21.37468905102145 88-89 21.333869068029042 30.158796353297173 27.171722387046692 21.335612191627092 90-91 21.045782178689702 30.539920756224888 27.13729711075969 21.276999954325717 92-93 20.822229194716616 30.642369730195355 27.00698617460273 21.528414900485295 94-95 20.805526098973235 30.80419338169067 26.813036093249607 21.577244426086484 96-97 20.763757432909365 30.466494014506928 26.926676082586194 21.843072469997512 98-99 21.150005571411707 30.492501762441627 26.402269164277207 21.955223501869455 100 20.879752273452404 30.921423959873735 26.21291614869418 21.98590761797968 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 50437.0 1 27806.0 2 3897.5 3 2315.5 4 1980.0 5 1852.5 6 1775.0 7 1937.5 8 1951.5 9 1800.0 10 1794.0 11 2668.0 12 17531.0 13 19755.0 14 7122.5 15 6508.0 16 7212.5 17 7954.5 18 8719.5 19 9753.0 20 10709.5 21 11902.0 22 13454.5 23 15312.5 24 17113.5 25 18930.5 26 21198.0 27 23671.5 28 26124.5 29 28904.0 30 31448.0 31 33991.0 32 37183.0 33 40541.0 34 44022.0 35 47605.0 36 50966.0 37 54044.0 38 56670.0 39 58570.0 40 60056.5 41 61239.5 42 62610.5 43 64203.0 44 66494.5 45 69493.0 46 71338.0 47 72717.0 48 73595.0 49 73995.5 50 78179.5 51 77671.0 52 70348.5 53 66693.0 54 65454.5 55 63418.0 56 58880.0 57 53076.5 58 46532.0 59 39033.0 60 32498.0 61 27403.5 62 23012.0 63 18541.0 64 14814.5 65 11635.0 66 9208.0 67 7695.5 68 6254.0 69 4798.0 70 3496.0 71 2479.0 72 1891.0 73 1448.5 74 1112.0 75 917.0 76 716.5 77 491.0 78 307.5 79 183.0 80 89.5 81 45.5 82 28.0 83 15.5 84 10.5 85 6.5 86 3.0 87 1.5 88 1.0 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13234500431809224 2 0.00525143564983427 3 0.0036627660414810456 4 0.0 5 0.0022506152785004016 6 0.01204741119667862 7 0.0024271341238729822 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 9.70853649549193E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.7651884537258052E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.2064855671572566E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.0890797940201595E-4 74-75 3.309728350735885E-4 76-77 5.516213917893142E-4 78-79 3.309728350735885E-4 80-81 8.605293711913301E-4 82-83 0.0011253076392502008 84-85 0.0011253076392502008 86-87 0.0 88-89 0.0 90-91 6.61945670147177E-5 92-93 0.0 94-95 0.0 96-97 0.0011694373505933462 98-99 6.398808144756044E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2266047.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.71946224171178 #Duplication Level Percentage of deduplicated Percentage of total 1 91.4679207741331 67.42965931836589 2 5.858576200450774 8.637821739986443 3 1.1820845094604615 2.614279030850486 4 0.46516208723189834 1.3716599570387107 5 0.24279438100182926 0.8949335601382067 6 0.16478295569155987 0.7288626528108967 7 0.11478340555351944 0.5923239652174437 8 0.08188469986430721 0.48291968318565576 9 0.06751670781481253 0.44795658531948823 >10 0.3277417505976502 3.786630429607654 >50 0.014112228530222147 0.717806805790865 >100 0.011023859328966783 1.7003097886089749 >500 6.585497685450542E-4 0.3324209498977865 >1k 7.184179293217637E-4 1.273941943547322 >5k 5.986816077681363E-5 0.22901384878112085 >10k+ 1.7960448233044092E-4 8.759459740853078 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 139816 6.17003972115318 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47894 2.1135483950685927 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10762 0.47492395347492794 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 5189 0.22898907215958011 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4692 0.20705660562203698 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4068 0.17951966574391442 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3956 0.17457713807348213 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3892 0.17175283654752085 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3301 0.14567217714372208 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.028066496414240305 0.0 0.0 0.0 0.0 2 0.031067316785574173 0.0 0.0 0.0 0.0 3 0.03411226686825119 4.412971134314513E-5 0.0 0.0 0.0 4 0.034774212538398366 4.412971134314513E-5 0.0 0.0 0.0 5 0.03481834224974151 4.412971134314513E-5 0.0 0.0 0.0 6 0.0349066016724278 8.825942268629026E-5 0.0 0.0 0.0 7 0.03499486109511409 8.825942268629026E-5 0.0 0.0 0.0 8 0.03552441763123183 1.323891340294354E-4 0.0 0.0 0.0 9 0.036009844456006426 1.323891340294354E-4 0.0 0.0 0.0 10-11 0.03620842815705058 1.7651884537258055E-4 0.0 0.0 0.0 12-13 0.03625255786839372 2.4271341238729822E-4 0.0 0.0 0.0 14-15 0.03634081729108002 2.647782680588708E-4 0.0 0.0 0.0 16-17 0.03636288214675159 2.647782680588708E-4 0.0 0.0 0.0 18-19 0.03636288214675159 2.647782680588708E-4 0.0 0.0 0.0 20-21 0.03638494700242316 2.647782680588708E-4 0.0 0.0 0.0 22-23 0.03645114156943788 2.868431237304434E-4 0.0 0.0 0.0 24-25 0.03645114156943788 3.5303769074516105E-4 0.0 0.0 0.0 26-27 0.036495271280781026 3.971674020883062E-4 0.0 0.0 0.0 28-29 0.036583530703467315 4.8542682477459645E-4 0.0 0.0 0.0 30-31 0.03678211440451147 5.295565361177416E-4 0.0 0.0 0.0 32-33 0.03691450353854091 5.736862474608868E-4 0.0 0.0 2.2064855671572566E-5 34-35 0.03722341151794292 5.736862474608868E-4 0.0 0.0 4.412971134314513E-5 36-37 0.03786329233241853 5.957511031324593E-4 0.0 0.0 4.412971134314513E-5 38-39 0.039010664827340294 6.178159588040319E-4 0.0 0.0 4.412971134314513E-5 40-41 0.04040075073464937 6.178159588040319E-4 0.0 0.0 4.412971134314513E-5 42-43 0.042739625435836065 6.398808144756044E-4 0.0 0.0 4.412971134314513E-5 44-45 0.045144694704037466 6.840105258187495E-4 0.0 0.0 4.412971134314513E-5 46-47 0.0477704125289546 7.060753814903221E-4 0.0 0.0 4.412971134314513E-5 48-49 0.050859492322974764 7.281402371618947E-4 0.0 0.0 4.412971134314513E-5 50-51 0.0548532311995294 7.502050928334672E-4 0.0 0.0 4.412971134314513E-5 52-53 0.061296169055628597 7.943348041766124E-4 0.0 4.412971134314513E-5 4.412971134314513E-5 54-55 0.06758465292202677 8.16399659848185E-4 0.0 4.412971134314513E-5 4.412971134314513E-5 56-57 0.07539561182976345 8.384645155197576E-4 0.0 4.412971134314513E-5 4.412971134314513E-5 58-59 0.08543512116032898 8.384645155197576E-4 0.0 4.412971134314513E-5 4.412971134314513E-5 60-61 0.09790176461476748 8.384645155197576E-4 0.0 4.412971134314513E-5 4.412971134314513E-5 62-63 0.11204533710024549 8.384645155197576E-4 0.0 4.412971134314513E-5 4.412971134314513E-5 64-65 0.12993993504989082 8.384645155197576E-4 0.0 8.825942268629026E-5 4.412971134314513E-5 66-67 0.1503278616904239 9.046590825344753E-4 0.0 8.825942268629026E-5 4.412971134314513E-5 68-69 0.176960142486012 9.267239382060477E-4 0.0 8.825942268629026E-5 4.412971134314513E-5 70-71 0.21091795536456215 9.267239382060477E-4 0.0 1.1032427835786282E-4 4.412971134314513E-5 72-73 0.25248814344980486 9.487887938776203E-4 0.0 1.323891340294354E-4 4.412971134314513E-5 74-75 0.30067778823651936 9.929185052207656E-4 0.0 1.323891340294354E-4 4.412971134314513E-5 76-77 0.3551117871782889 0.0010370482165639105 0.0 1.323891340294354E-4 4.412971134314513E-5 78-79 0.4212401596259919 0.0010591130722354832 0.0 1.323891340294354E-4 4.412971134314513E-5 80-81 0.5024608933530506 0.0010591130722354832 0.0 1.323891340294354E-4 4.412971134314513E-5 82-83 0.6083501357209272 0.0010591130722354832 0.0 1.323891340294354E-4 4.412971134314513E-5 84-85 0.7363262986160481 0.0010591130722354832 0.0 1.323891340294354E-4 4.412971134314513E-5 86-87 0.8836312750794666 0.0011032427835786284 0.0 1.323891340294354E-4 4.412971134314513E-5 88 1.0116736325416023 0.0011032427835786284 0.0 1.323891340294354E-4 4.412971134314513E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2590 0.0 85.625336 1 AACGAGT 32690 0.0 79.521385 1 ACGAGTG 36280 0.0 77.92675 2 CGAGTGG 37910 0.0 77.15934 3 GTGGAAT 40530 0.0 76.88661 6 GGAATCA 41050 0.0 75.98776 8 GAATCAC 41070 0.0 75.92787 9 TGGAATC 41125 0.0 75.7349 7 GAGTGGA 39805 0.0 75.51379 4 AGTGGAA 40395 0.0 74.4574 5 AACGTGT 1320 0.0 70.98396 1 ATCGAGT 1020 0.0 66.47271 1 TCGAGTG 1110 0.0 66.06814 2 CGTGTGG 1650 0.0 63.527744 3 ACGTGTG 1895 0.0 56.560867 2 ATGAGTG 1945 0.0 45.680687 2 TGAGTGG 2180 0.0 43.98617 3 AATGAGT 2000 0.0 42.376358 1 AGTACTT 23815 0.0 41.018944 20-21 GAGTACT 23505 0.0 40.540173 20-21 >>END_MODULE