FastQCFastQC Report
Thu 26 May 2016
SRR2132761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110702
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1587057.519062378298784No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1540437.298187996221163No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG173200.8205800724119274No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125680.5954417061243131No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122820.5818917118570031No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC63660.3016058164534833No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48560.2300656369302725No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42670.20216022915598694No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42630.20197071874665395No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36420.17254922769770437No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.16928017313670998No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34620.164021259277719No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33030.1564882205067319No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA30410.14407528869541983No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.1345050130241029No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22670.10740502448948264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT30200.085.880761
AACGTGT13650.075.175281
CGTGTGG15350.073.501813
ATCGAGT10800.071.0419851
ACGTGTG15900.067.123622
TCGAGTG11850.066.258982
GTGGAAT544150.066.206586
TGGAATC548900.065.5713967
GGAATCA554050.064.968848
GAATCAC555000.064.8576359
ACGAGTG517200.063.815362
AACGAGT477650.063.5822371
AGTGGAA549050.063.545335
CGAGTGG535950.063.5403143
GAGTGGA553500.063.034444
AGCGAGT6650.051.671661
ATGAGTG18300.050.0991252
TGAGTGG19450.049.790073
GCGAGTG7350.049.2549672
AATGAGT16850.049.1658971
Produced by FastQC (version 0.11.5)