##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132761_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2110702 Sequences flagged as poor quality 0 Sequence length 100 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.013452870182526 31.0 25.0 33.0 16.0 34.0 2 28.304048605629784 31.0 26.0 34.0 16.0 34.0 3 29.364789534477154 31.0 28.0 34.0 16.0 34.0 4 34.80915685871336 35.0 35.0 37.0 32.0 37.0 5 33.946359552414314 35.0 35.0 37.0 28.0 37.0 6 35.29599630833722 37.0 35.0 37.0 32.0 37.0 7 35.6689854844502 37.0 35.0 37.0 33.0 37.0 8 35.9252111382848 37.0 35.0 37.0 35.0 37.0 9 37.884660174671744 39.0 38.0 39.0 35.0 39.0 10-11 37.875429596409155 39.0 38.0 39.0 35.0 39.0 12-13 37.8294510546728 39.0 38.0 39.0 35.0 39.0 14-15 39.240176964820236 41.0 39.0 41.0 36.0 41.0 16-17 39.071441397222344 40.5 39.0 41.0 36.0 41.0 18-19 38.615570317363606 40.0 38.0 41.0 35.0 41.0 20-21 38.396940686084534 40.0 38.0 41.0 35.0 41.0 22-23 38.17690986221646 40.0 38.0 41.0 34.5 41.0 24-25 37.91487524055978 40.0 37.0 41.0 34.0 41.0 26-27 37.62470116577328 40.0 37.0 41.0 33.5 41.0 28-29 37.43878766400942 40.0 37.0 41.0 33.0 41.0 30-31 37.305947263043294 40.0 36.5 41.0 33.0 41.0 32-33 37.152600414459265 40.0 36.0 41.0 33.0 41.0 34-35 37.04002128201897 40.0 36.0 41.0 33.0 41.0 36-37 36.84972393071121 39.5 35.0 41.0 32.5 41.0 38-39 36.36070842781217 39.0 35.0 41.0 32.0 41.0 40-41 36.12535213402934 39.0 35.0 41.0 31.0 41.0 42-43 35.97458902298856 39.0 35.0 41.0 31.0 41.0 44-45 35.818704866911574 39.0 35.0 41.0 31.0 41.0 46-47 35.66361144301754 38.0 35.0 41.0 30.0 41.0 48-49 35.487268453813 38.0 35.0 41.0 30.0 41.0 50-51 35.204917842499796 38.0 35.0 40.5 28.5 41.0 52-53 34.75142417072614 38.0 35.0 40.0 26.0 41.0 54-55 33.92150099824608 37.0 34.5 40.0 19.5 41.0 56-57 33.23028167879691 37.0 34.0 40.0 4.5 41.0 58-59 32.88941593839395 37.0 34.0 40.0 2.0 41.0 60-61 32.644952248114606 36.0 34.0 40.0 2.0 41.0 62-63 32.40036182274902 35.5 33.0 40.0 2.0 41.0 64-65 32.1402613917076 35.0 33.0 39.5 2.0 41.0 66-67 31.83812281411587 35.0 33.0 39.0 2.0 41.0 68-69 31.549422182761944 35.0 33.0 39.0 2.0 41.0 70-71 31.259805031690878 35.0 33.0 38.0 2.0 40.0 72-73 30.98660682559641 35.0 33.0 37.0 2.0 40.0 74-75 30.69611318888218 35.0 33.0 37.0 2.0 39.5 76-77 30.394674615364934 35.0 32.5 36.0 2.0 39.0 78-79 30.094038855319226 35.0 32.0 36.0 2.0 39.0 80-81 29.80957425538991 35.0 32.0 35.5 2.0 37.5 82-83 29.536238180472658 35.0 31.5 35.0 2.0 37.0 84-85 29.29401260812753 35.0 31.0 35.0 2.0 37.0 86-87 29.105765996336764 35.0 31.0 35.0 2.0 36.0 88-89 28.889361454151274 35.0 31.0 35.0 2.0 36.0 90-91 28.69796399491733 35.0 31.0 35.0 2.0 36.0 92-93 28.539810925464607 35.0 31.0 35.0 2.0 35.0 94-95 28.34513991079745 35.0 31.0 35.0 2.0 35.0 96-97 28.137661782667568 34.5 30.0 35.0 2.0 35.0 98-99 27.97531413719227 34.0 30.0 35.0 2.0 35.0 100 27.876208484191515 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14531080015795084 1101 2 2.176396792616657 1101 3 1.128758208193215 1101 4 0.6663263648659736 1101 5 2.2370248495381517 1101 6 0.9991830100496983 1101 7 0.5833662951544909 1101 8 0.4343253481469347 1101 9 0.3955645646691792 1101 10-11 0.48757297390734067 1101 12-13 0.39318158475715137 1101 14-15 0.49405899539128484 1101 16-17 0.4236388976979413 1101 18-19 0.356900147065744 1101 20-21 0.35066970926106933 1101 22-23 0.27669568135522127 1101 24-25 0.17508349773829934 1101 26-27 0.07885863442619012 1101 28-29 0.14242482421209246 1101 30-31 0.18134107080937412 1101 32-33 0.1402863123750251 1101 34-35 0.1364160913027277 1101 36-37 0.10436696887621366 1101 38-39 0.14481184522231416 1101 40-41 0.10973033487746875 1101 42-43 0.16241326785779364 1101 44-45 0.18707736503817785 1101 46-47 0.13603074662695036 1101 48-49 0.13246907764207094 1101 50-51 0.14762706116917457 1101 52-53 0.2029167430851473 1101 54-55 0.29727983041115635 1101 56-57 0.24486361188144912 1101 58-59 0.14735561830519117 1101 60-61 0.19548097638051587 1101 62-63 0.11806651350794084 1101 64-65 0.14859777493897042 1101 66-67 0.20248157766102182 1101 68-69 0.3373117867566009 1101 70-71 0.22404647901240082 1101 72-73 0.2265744430804979 1101 74-75 0.2605168877560722 1101 76-77 0.15329950190076147 1101 78-79 0.17095458875328262 1101 80-81 0.1971897218432126 1101 82-83 0.1185989663067204 1101 84-85 0.16241616969310613 1101 86-87 0.11705612837696222 1101 88-89 0.11084594237460976 1101 90-91 0.12237128519471696 1101 92-93 0.13548828778750988 1101 94-95 0.13286138385689128 1101 96-97 0.1828904514297669 1101 98-99 0.20432850591376805 1101 100 0.1595966952079486 1102 1 1.7800394162420652 1102 2 3.13026436863257 1102 3 3.2422776739505323 1102 4 1.4421646712030025 1102 5 2.6011410757387807 1102 6 1.2410144962673755 1102 7 0.7593637376097391 1102 8 0.5637243251290371 1102 9 0.5115961077211821 1102 10-11 0.5583750483603112 1102 12-13 0.48947811786315043 1102 14-15 0.6209188987729277 1102 16-17 0.4517306851375622 1102 18-19 0.3367424318057317 1102 20-21 0.25614995933210594 1102 22-23 0.2738411772347362 1102 24-25 0.2679543417280712 1102 26-27 0.41875491177770385 1102 28-29 0.41021823143005065 1102 30-31 0.4725281978340945 1102 32-33 0.483584834682496 1102 34-35 0.47004354228312195 1102 36-37 0.5084121521669189 1102 38-39 0.5122180685812197 1102 40-41 0.5775052709388504 1102 42-43 0.5306997128705788 1102 44-45 0.6016795247426359 1102 46-47 0.6392092064166661 1102 48-49 0.6536171310663406 1102 50-51 0.7133602239995156 1102 52-53 0.8451787493369167 1102 54-55 0.7633902310931688 1102 56-57 0.5783781046350711 1102 58-59 0.698726036037506 1102 60-61 0.6482232327033373 1102 62-63 0.6729066669606283 1102 64-65 0.6268423046349056 1102 66-67 0.6498402022702834 1102 68-69 0.5734527358898802 1102 70-71 0.6316949586941298 1102 72-73 0.6184783930662903 1102 74-75 0.6512180812769621 1102 76-77 0.6883954098291554 1102 78-79 0.678291900489576 1102 80-81 0.6733235666855002 1102 82-83 0.6460932828739274 1102 84-85 0.6162588806905482 1102 86-87 0.5972046080586892 1102 88-89 0.6770359111157305 1102 90-91 0.706133007274854 1102 92-93 0.7385964150964028 1102 94-95 0.7954366893414004 1102 96-97 0.7748427105942994 1102 98-99 0.8100829816170894 1102 100 0.6838280109226424 1103 1 1.5263428121769032 1103 2 3.1395464847135344 1103 3 3.2547553850600366 1103 4 1.4237527735433915 1103 5 2.5908150296573567 1103 6 1.2294672219662885 1103 7 0.7299630989054648 1103 8 0.5529909637055397 1103 9 0.42842410141118137 1103 10-11 0.49577198828716007 1103 12-13 0.49456768536635565 1103 14-15 0.5554708921735738 1103 16-17 0.41138377560981354 1103 18-19 0.2374549505195418 1103 20-21 0.15868782023827066 1103 22-23 0.09314406424472566 1103 24-25 0.1658804550280948 1103 26-27 0.3160324469767559 1103 28-29 0.36955967422282754 1103 30-31 0.2838643492759658 1103 32-33 0.3045622447518994 1103 34-35 0.354512457550598 1103 36-37 0.3307131576464144 1103 38-39 0.31779681327633114 1103 40-41 0.3970774151634089 1103 42-43 0.4544754758451077 1103 44-45 0.5157885469569123 1103 46-47 0.5450163172795754 1103 48-49 0.579139596537253 1103 50-51 0.6133835287856897 1103 52-53 0.6465627122783175 1103 54-55 0.7348973784126684 1103 56-57 0.7347430508869124 1103 58-59 0.5998609839092026 1103 60-61 0.664555285868559 1103 62-63 0.6485364165968832 1103 64-65 0.6970984188114571 1103 66-67 0.6864721837266359 1103 68-69 0.6251610875771263 1103 70-71 0.6032978534325046 1103 72-73 0.6978724239624405 1103 74-75 0.6944233363866026 1103 76-77 0.6953785909037506 1103 78-79 0.6362742962040429 1103 80-81 0.6181772406228312 1103 82-83 0.6126063708402434 1103 84-85 0.644427404442407 1103 86-87 0.5844585569318781 1103 88-89 0.6477786742099809 1103 90-91 0.6375116576501831 1103 92-93 0.5972734242389492 1103 94-95 0.6071506064221417 1103 96-97 0.601799170123531 1103 98-99 0.6134326976887081 1103 100 0.6442003834415928 1104 1 5.982594508835305 1104 2 5.023146559699896 1104 3 4.044978049488556 1104 4 1.8194980486132053 1104 5 2.7747967196187915 1104 6 1.228394466341662 1104 7 0.7292703334492217 1104 8 0.5611192890985208 1104 9 0.4755881776097084 1104 10-11 0.45042781054434045 1104 12-13 0.3392694666603191 1104 14-15 0.4438649516350637 1104 16-17 0.2657443045794281 1104 18-19 0.09869649750105935 1104 20-21 0.10105378096730533 1104 22-23 0.03655898331172125 1104 24-25 0.049326518267100994 1104 26-27 0.06099263384790987 1104 28-29 0.07131437347800329 1104 30-31 0.14366276901350972 1104 32-33 0.17141611929505984 1104 34-35 0.23719371201785577 1104 36-37 0.21882724452114388 1104 38-39 0.26184954561104234 1104 40-41 0.24304064425517424 1104 42-43 0.2911359202174779 1104 44-45 0.37773194145093214 1104 46-47 0.2788924101345174 1104 48-49 0.41674350177546415 1104 50-51 0.4682536875706873 1104 52-53 0.3578291985096982 1104 54-55 0.4497118829700213 1104 56-57 0.47933167819044087 1104 58-59 0.536326367172741 1104 60-61 0.48403592867273915 1104 62-63 0.45678121457132903 1104 64-65 0.4277156629461629 1104 66-67 0.5120573478926502 1104 68-69 0.46999691542303523 1104 70-71 0.5746342324097746 1104 72-73 0.5184956047729194 1104 74-75 0.6056981612485401 1104 76-77 0.5724720048724592 1104 78-79 0.618405676233607 1104 80-81 0.6183110955702027 1104 82-83 0.5769657379197888 1104 84-85 0.5077961452770019 1104 86-87 0.5134468020686107 1104 88-89 0.5221911571399289 1104 90-91 0.5579576732179738 1104 92-93 0.6161606140637339 1104 94-95 0.6391543931414319 1104 96-97 0.5564372114773128 1104 98-99 0.5624966557515272 1104 100 0.6129301356294476 1105 1 1.6740040068426616 1105 2 3.0856736959930515 1105 3 3.1827971591276807 1105 4 1.3294603508935623 1105 5 2.4911991480809377 1105 6 1.1705109564941907 1105 7 0.7267414754828962 1105 8 0.49204964425833 1105 9 0.4098174782898454 1105 10-11 0.4446007971612005 1105 12-13 0.4170987633673704 1105 14-15 0.4506300812519157 1105 16-17 0.32011313302447775 1105 18-19 0.2298525889959535 1105 20-21 0.13672477223417445 1105 22-23 0.11772116236603125 1105 24-25 0.012932808032822152 1105 26-27 0.07616732422892625 1105 28-29 0.10581864522906415 1105 30-31 0.1326898867204207 1105 32-33 0.17402470246790358 1105 34-35 0.17027965767168496 1105 36-37 0.12818239364077044 1105 38-39 0.1616235985816914 1105 40-41 0.13326136757162033 1105 42-43 0.18949899881591392 1105 44-45 0.3389146075130114 1105 46-47 0.3088087765493057 1105 48-49 0.21963844855935832 1105 50-51 0.13791453300551382 1105 52-53 0.3077931472152713 1105 54-55 0.2974767328149923 1105 56-57 0.2824483054447384 1105 58-59 0.28114806639492684 1105 60-61 0.3466877583492973 1105 62-63 0.39457158701709716 1105 64-65 0.4086847783267089 1105 66-67 0.3917248467515151 1105 68-69 0.47958173634570045 1105 70-71 0.46479024248637657 1105 72-73 0.42839912335216823 1105 74-75 0.45263742817394714 1105 76-77 0.3429704127010176 1105 78-79 0.4081701561596347 1105 80-81 0.4731400727744379 1105 82-83 0.4698643853980293 1105 84-85 0.458821880227525 1105 86-87 0.5246541946081358 1105 88-89 0.5098799899975219 1105 90-91 0.4721168397957243 1105 92-93 0.5944562825356989 1105 94-95 0.6792453739368014 1105 96-97 0.6825623753109191 1105 98-99 0.5902807209138494 1105 100 0.5781561752137847 1106 1 -0.7211954387012298 1106 2 1.8682684503706675 1106 3 2.7047548051791175 1106 4 1.1922662449502397 1106 5 2.361384758791111 1106 6 1.1073251264003687 1106 7 0.6208667002752222 1106 8 0.48946957112538314 1106 9 0.4116778364241611 1106 10-11 0.4432888222301443 1106 12-13 0.38357333649917535 1106 14-15 0.40086988503639986 1106 16-17 0.3454984018301772 1106 18-19 0.317236397268303 1106 20-21 0.34168656228327166 1106 22-23 0.2413440365650814 1106 24-25 0.22715163904440772 1106 26-27 0.15601063572257345 1106 28-29 0.25925921085931236 1106 30-31 0.324589733910976 1106 32-33 0.27890363533726514 1106 34-35 0.3559048289465494 1106 36-37 0.3371517687465726 1106 38-39 0.38008530171234867 1106 40-41 0.28829944847332456 1106 42-43 0.3243157147869695 1106 44-45 0.4344789207017641 1106 46-47 0.41875072718115547 1106 48-49 0.4464587065514891 1106 50-51 0.4875614316115602 1106 52-53 0.5408571093142811 1106 54-55 0.40349964891706236 1106 56-57 0.2985805945422797 1106 58-59 0.3024767494022669 1106 60-61 0.3562166756188958 1106 62-63 0.31379532569397384 1106 64-65 0.2468448175576654 1106 66-67 0.20145986319010944 1106 68-69 0.27918020354480433 1106 70-71 0.29629327855866805 1106 72-73 0.4121910368254369 1106 74-75 0.37355326759726637 1106 76-77 0.36813056655802967 1106 78-79 0.3667299898228009 1106 80-81 0.35200822775797747 1106 82-83 0.3442629591765929 1106 84-85 0.4045005158282535 1106 86-87 0.3649952792439173 1106 88-89 0.40951755151413494 1106 90-91 0.4678205640798261 1106 92-93 0.49540361755578033 1106 94-95 0.5265046345455922 1106 96-97 0.5155545253237825 1106 98-99 0.5347773796781468 1106 100 0.5780588569321452 1107 1 -0.9113238234501431 1107 2 1.7714538372431718 1107 3 -0.6477316229820573 1107 4 0.05605961495577105 1107 5 1.8916016759069691 1107 6 0.7774510599273938 1107 7 0.312037334957445 1107 8 0.2807895220689076 1107 9 0.2943510621120069 1107 10-11 0.33770027664582614 1107 12-13 0.3217183943632236 1107 14-15 0.36866070929580985 1107 16-17 0.3074001655083549 1107 18-19 0.2897209910183278 1107 20-21 0.2250799345317489 1107 22-23 0.21042359473634065 1107 24-25 0.09486716433085007 1107 26-27 0.08854535936333008 1107 28-29 0.06599366262270934 1107 30-31 0.08461643300426402 1107 32-33 0.15119829944377017 1107 34-35 0.11119834674633466 1107 36-37 0.10341414401262483 1107 38-39 0.0958961635090887 1107 40-41 0.22143688431899733 1107 42-43 0.18409364690094066 1107 44-45 0.26005249460730795 1107 46-47 0.23539692694613024 1107 48-49 0.13329592539192703 1107 50-51 0.1696196889850512 1107 52-53 0.1941802772862644 1107 54-55 0.05656215427410416 1107 56-57 0.005495763840805523 1107 58-59 0.034159793595549104 1107 60-61 0.0451192091089041 1107 62-63 0.03513205068530567 1107 64-65 0.003776648756598888 1107 66-67 -0.005699066507084183 1107 68-69 -0.08600791972441613 1107 70-71 -0.028127460060172638 1107 72-73 -0.06426814111805257 1107 74-75 -0.07924813456573787 1107 76-77 -0.11688946376983012 1107 78-79 -0.06194245724161718 1107 80-81 -0.05793480135949736 1107 82-83 -0.019433763796079973 1107 84-85 -0.09309872861864577 1107 86-87 -0.10554239547573374 1107 88-89 -0.1506393987813297 1107 90-91 -0.05372167103400827 1107 92-93 -0.10978243899695173 1107 94-95 -0.18400325702708997 1107 96-97 -0.13546201081131315 1107 98-99 -0.12296311442730357 1107 100 -0.06850843111126181 1108 1 -1.8503583896183642 1108 2 0.06360496613418576 1108 3 1.8241359957767997 1108 4 0.7559983040397995 1108 5 0.6181108615540367 1108 6 0.35268747357775254 1108 7 0.14798952586627934 1108 8 0.10589946307854348 1108 9 0.10175189707472754 1108 10-11 0.21689809864852805 1108 12-13 0.2029209859994765 1108 14-15 0.3502580381936369 1108 16-17 0.22440923814802716 1108 18-19 0.1783160867826581 1108 20-21 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-0.13397590243320678 2215 36-37 -0.12346850141839383 2215 38-39 -0.06865027673129731 2215 40-41 -0.061200466727697744 2215 42-43 -0.052943490486150324 2215 44-45 -0.08592302646618322 2215 46-47 -0.15681891570054063 2215 48-49 -0.24229259408152615 2215 50-51 -0.21006648879657064 2215 52-53 -0.13308599744534177 2215 54-55 -0.02253249218843223 2215 56-57 -0.047858077459849824 2215 58-59 0.03438385555683254 2215 60-61 0.005709027667585076 2215 62-63 -0.1538726565028199 2215 64-65 -0.014133212630767389 2215 66-67 -0.07798578066334017 2215 68-69 -0.07981261375235249 2215 70-71 -0.09948831593719021 2215 72-73 -0.059201224923807416 2215 74-75 -0.008555980992092316 2215 76-77 0.040718264746999466 2215 78-79 -0.024958105775439776 2215 80-81 -0.05376016559928232 2215 82-83 0.0122279669918548 2215 84-85 0.040987912866246035 2215 86-87 -0.03903886036728821 2215 88-89 -0.17182827706357884 2215 90-91 -0.1551443749795034 2215 92-93 -0.28734987409954016 2215 94-95 -0.19409785075209385 2215 96-97 -0.2404588683314195 2215 98-99 -0.3078130229107394 2215 100 -0.40539204904182924 2216 1 2.878338965589588 2216 2 -1.791245687840501 2216 3 -1.9573010901297465 2216 4 -0.8163059414436944 2216 5 -2.4124626277819736 2216 6 -0.9715097646098911 2216 7 -0.5776489999805676 2216 8 -0.39682136305988536 2216 9 -0.3069585391240821 2216 10-11 -0.189589159611387 2216 12-13 -0.25468909334977496 2216 14-15 -0.29692772734623674 2216 16-17 -0.33836600284976726 2216 18-19 -0.18684266744259048 2216 20-21 -0.08143828931454777 2216 22-23 -0.07748360408048427 2216 24-25 0.11402907452767863 2216 26-27 0.16308132955786192 2216 28-29 0.11236793377070597 2216 30-31 0.09437012647891407 2216 32-33 0.1269839960607797 2216 34-35 0.11245334563945164 2216 36-37 0.04259200406180952 2216 38-39 0.07022631455573247 2216 40-41 0.09733822323315167 2216 42-43 0.08239359783930666 2216 44-45 0.021314982252832237 2216 46-47 -0.01168269850895598 2216 48-49 0.1140732015631869 2216 50-51 0.05477278887506998 2216 52-53 -0.013495174232950546 2216 54-55 0.08383889133733646 2216 56-57 0.36485432797186235 2216 58-59 0.391785399730594 2216 60-61 0.3699235178825617 2216 62-63 0.35618951047266023 2216 64-65 0.2843403605289865 2216 66-67 0.22017755717707388 2216 68-69 0.4205475047970637 2216 70-71 0.23923147892761776 2216 72-73 0.22334194685341302 2216 74-75 0.118558576325416 2216 76-77 0.09058720135644904 2216 78-79 0.09163761318819752 2216 80-81 0.09835644220677153 2216 82-83 0.07747705458440635 2216 84-85 0.17915870441238368 2216 86-87 0.15492171941014377 2216 88-89 0.13196251266949233 2216 90-91 0.07597297579889073 2216 92-93 0.051443258706406425 2216 94-95 0.004503788007937715 2216 96-97 -0.05895988686063802 2216 98-99 -0.09151789837294189 2216 100 -0.143515067802241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1083.0 3 320.0 4 487.0 5 1711.0 6 5330.0 7 9498.0 8 11542.0 9 15338.0 10 10094.0 11 5700.0 12 5002.0 13 6760.0 14 6697.0 15 7284.0 16 8328.0 17 9520.0 18 13388.0 19 21941.0 20 43140.0 21 51348.0 22 22719.0 23 14661.0 24 14813.0 25 15818.0 26 16984.0 27 18570.0 28 22642.0 29 26224.0 30 30721.0 31 37932.0 32 49839.0 33 73380.0 34 107033.0 35 268823.0 36 267081.0 37 396149.0 38 387610.0 39 104695.0 40 497.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.929312577986742 44.77618100990165 24.100240543143574 18.19426586896804 2 13.849277326213597 44.58114296408782 23.73321835622215 17.836361353476438 3 17.112529013862694 28.448296692597324 18.72579216906429 35.71338212447569 4 31.715893574744324 30.16678811125398 20.608025197304023 17.509293116697666 5 15.805911646997343 43.281467720561515 21.21051033166607 19.702110300775068 6 35.39081020199967 27.002689948916082 22.615329723674915 14.991170125409331 7 17.675530798463594 26.586567777277143 38.92324653979526 16.81465488446401 8 34.6916807772959 27.005991371591065 22.024331241454263 16.277996609658775 9 33.97296254990046 28.691260064187173 22.12173959185143 15.214037794060934 10-11 15.347287300623206 46.521015283066966 22.18065364035283 15.951043775957006 12-13 15.21877555429426 28.32299396125081 31.015084081030857 25.443146403424073 14-15 15.466631480900666 37.10118718795927 22.07947877057017 25.352702560569895 16-17 24.898706686211508 27.838178956574637 31.134641460518825 16.128472896695033 18-19 15.80763177369425 28.069097390346908 31.33701488888531 24.78625594707353 20-21 24.93786427453994 37.369960325995805 22.162389574653364 15.529785824810894 22-23 24.44189184451429 28.347914580078097 31.40523863624519 15.804954939162421 24-25 15.628212793658225 37.33478245626337 21.850123797674897 25.186880952403513 26-27 16.368878483707185 31.977156399024874 35.436173914773796 16.217791202494148 28-29 24.1055819343517 28.296059794324353 31.501557301788697 16.096800969535256 30-31 21.824918913233606 29.570493608287666 31.776205262514555 16.828382215964165 32-33 18.116555534604124 31.050569905178467 32.55793570101322 18.27493885920419 34-35 18.13043717208777 30.975381650275597 32.719422258566105 18.174758919070527 36-37 18.45762689380121 30.694764111655743 33.118768068633095 17.728840925909957 38-39 19.208017048356425 30.200307764904753 32.65458600977305 17.937089176965767 40-41 17.57751686405755 31.94385090837077 32.680691068658675 17.797941158913005 42-43 17.490629224784662 30.710013741987492 33.50614457063573 18.293212462592113 44-45 17.833332227855944 31.359400805987768 32.32732522165611 18.479941744500174 46-47 18.60414212901679 30.345472738453843 33.01934617013676 18.031038962392607 48-49 17.73260744529545 30.773174043517276 32.9409599270764 18.55325858411088 50-51 18.374029114484188 31.618887933966995 31.97249066898122 18.0345922825676 52-53 18.669114511773348 31.007580423556163 32.014000094755296 18.309304969915193 54-55 18.698565690466964 32.110501624577985 30.006770259373422 19.18416242558163 56-57 19.01265550513526 32.80759197650829 28.924500000473774 19.255252517882678 58-59 20.15215790765347 32.95481787575887 27.660441881421445 19.232582335166214 60-61 19.682906445343775 33.48582604270996 27.280402444305256 19.550865067641002 62-63 18.572104379564983 33.61433798873881 27.035976918563293 20.777580713132913 64-65 19.35140536181801 33.586598202872786 27.84393533525813 19.218061100051074 66-67 19.526470228026085 32.74477703265952 28.293152774089563 19.43559996522483 68-69 19.895371303007245 32.93956228780756 27.955935987173934 19.209130422011256 70-71 18.09393272949 34.191586495867256 28.244773539798608 19.46970723484414 72-73 18.149394535349288 33.306098992131965 28.519424662895076 20.025081809623664 74-75 18.526743835670956 33.75658314903359 27.556199578147826 20.160473437147626 76-77 19.529480546276726 32.820096380872876 27.8906622175655 19.7597608552849 78-79 19.242622798589277 33.28491319434574 27.2385075605536 20.23395644651138 80-81 19.671116015262356 33.95724655070265 26.895766229280703 19.475871204754288 82-83 19.352739554849727 34.00438863200754 26.900471198743723 19.742400614399006 84-85 19.272023835046017 34.342971568080046 26.124978590956864 20.26002600591707 86-87 19.100952716656693 34.50723243131073 26.536705104133652 19.855109747898922 88-89 20.65748741413994 33.56814462676398 26.282037919137803 19.49233003995827 90-91 19.609262607715966 34.39548415538625 26.237888209204378 19.7573650276934 92-93 18.808150084663776 34.53945654099916 26.380062178365304 20.272331195971766 94-95 19.25361799060218 34.840635959031644 26.079332847554987 19.826413202811196 96-97 19.298168312820955 33.76644081496127 26.60966126034443 20.325729611873346 98-99 20.195855569368852 33.79999791537167 25.543045679892057 20.461100835367425 100 19.156801860234175 35.009868754566014 25.30636726548797 20.52696211971183 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 171973.0 1 92335.0 2 8914.0 3 4209.5 4 3122.5 5 2728.5 6 2688.0 7 3053.5 8 2906.0 9 2496.0 10 2381.5 11 3592.5 12 24979.5 13 27038.5 14 7354.0 15 5847.0 16 6257.5 17 6878.0 18 7500.5 19 8341.0 20 9283.5 21 10277.0 22 11572.0 23 13230.5 24 15079.5 25 16942.0 26 19207.0 27 21583.0 28 23433.5 29 25273.0 30 27375.5 31 29335.0 32 31521.5 33 34293.5 34 37531.0 35 40585.5 36 43557.0 37 46139.0 38 47796.5 39 49049.5 40 49646.0 41 49709.5 42 49529.5 43 50076.5 44 51707.0 45 53310.5 46 55281.5 47 58493.5 48 61203.0 49 66068.0 50 84907.0 51 87009.5 52 66012.0 53 57111.5 54 56688.0 55 58227.0 56 53462.5 57 44983.5 58 38724.0 59 31196.5 60 25337.0 61 21172.5 62 17113.5 63 13195.5 64 10118.0 65 8011.0 66 6706.0 67 5616.0 68 4611.5 69 3595.0 70 2550.5 71 1921.5 72 1610.5 73 1177.5 74 827.0 75 659.0 76 497.5 77 370.0 78 239.5 79 141.0 80 82.5 81 49.5 82 35.0 83 20.5 84 9.0 85 3.5 86 3.0 87 3.5 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14080623413442542 2 0.006490731519655546 3 0.0034585649703274077 4 0.0 5 0.0026057681283288684 6 0.011986533390312797 7 0.0028900337423283817 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.106640349987824E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.291080408319128E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.475520466650432E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 7.106640349987824E-5 64-65 0.0 66-67 2.368880116662608E-5 68-69 0.0 70-71 0.0 72-73 4.2639842099926944E-4 74-75 4.7377602333252154E-4 76-77 6.395976314989042E-4 78-79 4.7377602333252154E-4 80-81 8.76485643165165E-4 82-83 0.0010186184501649214 84-85 0.0013502616664976864 86-87 4.737760233325216E-5 88-89 0.0 90-91 2.368880116662608E-5 92-93 0.0 94-95 0.0 96-97 0.0011844400583313041 98-99 6.632864326655302E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2110702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.64332386467726 #Duplication Level Percentage of deduplicated Percentage of total 1 88.59575570993002 51.06953839423325 2 7.824962898336893 9.02113741155834 3 1.6499333739259694 2.853229314850628 4 0.5570143072681689 1.2843262444447163 5 0.2910302597408457 0.8387975758331353 6 0.18008586624291736 0.622844874677486 7 0.12748115576861654 0.5143906284029612 8 0.08667434369713191 0.39969578115936977 9 0.06512869409859887 0.3378810966128125 >10 0.5364185365930146 6.545263692139097 >50 0.04961609258557951 1.980919233894974 >100 0.02964190168103196 3.2237683182492574 >500 0.0031284300656680333 1.1529929418232205 >1k 0.002634467423720449 3.0100917561287894 >5k 8.232710699126403E-5 0.3021054110819321 >10k+ 4.116355349563201E-4 16.84301732491002 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 158705 7.519062378298784 No Hit AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 154043 7.298187996221163 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 17320 0.8205800724119274 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12568 0.5954417061243131 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 12282 0.5818917118570031 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 6366 0.3016058164534833 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4856 0.2300656369302725 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4267 0.20216022915598694 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4263 0.20197071874665395 No Hit ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3642 0.17254922769770437 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3573 0.16928017313670998 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3462 0.164021259277719 No Hit AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3303 0.1564882205067319 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA 3041 0.14407528869541983 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2839 0.1345050130241029 No Hit AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2267 0.10740502448948264 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012791952629978083 0.0 0.0 0.0 0.0 2 0.0012791952629978083 0.0 0.0 0.0 0.0 3 0.0013265728653310605 0.0 0.0 0.0 0.0 4 0.0013265728653310605 4.737760233325216E-5 0.0 0.0 0.0 5 0.0013265728653310605 1.421328069997565E-4 0.0 0.0 0.0 6 0.0013265728653310605 1.421328069997565E-4 0.0 0.0 0.0 7 0.0013265728653310605 1.8951040933300864E-4 0.0 0.0 0.0 8 0.0013265728653310605 2.368880116662608E-4 0.0 0.0 0.0 9 0.0013265728653310605 3.3164321633276513E-4 0.0 0.0 0.0 10-11 0.0013739504676643125 4.974648244991476E-4 0.0 0.0 0.0 12-13 0.0013739504676643125 5.92220029165652E-4 0.0 0.0 0.0 14-15 0.0015397720758306953 7.343528361654085E-4 0.0 0.0 0.0 16-17 0.0016108384793305734 7.580416373320346E-4 0.0 0.0 0.0 18-19 0.00175297128633033 7.580416373320346E-4 0.0 0.0 0.0 20-21 0.0018714152921634603 7.580416373320346E-4 0.0 0.0 0.0 22-23 0.0019187928944967125 7.580416373320346E-4 0.0 0.0 0.0 24-25 0.002037236900329843 8.054192396652867E-4 0.0 0.0 0.0 26-27 0.002060925701496469 8.291080408319128E-4 0.0 0.0 0.0 28-29 0.002131992104996347 9.00174444331791E-4 0.0 0.0 0.0 30-31 0.002274124911996104 9.00174444331791E-4 0.0 0.0 0.0 32-33 0.0025347017248289905 9.475520466650432E-4 0.0 0.0 4.737760233325216E-5 34-35 0.0028900337423283817 0.0010659960524981736 0.0 0.0 4.737760233325216E-5 36-37 0.0036717641808270422 0.0010896848536647997 0.0 0.0 4.737760233325216E-5 38-39 0.004761449034491843 0.001137062455998052 0.0 0.0 4.737760233325216E-5 40-41 0.007319839560487458 0.001137062455998052 0.0 0.0 4.737760233325216E-5 42-43 0.010802093331981493 0.00120812885949793 0.0 0.0 4.737760233325216E-5 44-45 0.013573683068476745 0.0013502616664976866 0.0 0.0 4.737760233325216E-5 46-47 0.016392650407305247 0.0013739504676643125 0.0 0.0 4.737760233325216E-5 48-49 0.019543260962466516 0.0013739504676643125 0.0 0.0 4.737760233325216E-5 50-51 0.024209954792291852 0.0014213280699975647 0.0 0.0 4.737760233325216E-5 52-53 0.0307006863119474 0.0014213280699975647 0.0 0.0 4.737760233325216E-5 54-55 0.03847061309460076 0.001492394473497443 0.0 0.0 4.737760233325216E-5 56-57 0.04735391353208553 0.0015397720758306953 0.0 0.0 4.737760233325216E-5 58-59 0.05630828037307019 0.0016108384793305734 0.0 4.737760233325216E-5 4.737760233325216E-5 60-61 0.06836588016688286 0.00175297128633033 0.0 4.737760233325216E-5 4.737760233325216E-5 62-63 0.08459270896602172 0.00175297128633033 0.0 7.106640349987824E-5 4.737760233325216E-5 64-65 0.10375694910982222 0.001776660087496956 0.0 9.475520466650432E-5 4.737760233325216E-5 66-67 0.12723255106594866 0.001824037689830208 0.0 1.1844400583313041E-4 4.737760233325216E-5 68-69 0.15610919968806586 0.0018477264909968342 0.0 1.421328069997565E-4 4.737760233325216E-5 70-71 0.19761197933199476 0.0019661704968299645 0.0 1.421328069997565E-4 4.737760233325216E-5 72-73 0.24451580564191439 0.002084614502663095 0.0 1.421328069997565E-4 4.737760233325216E-5 74-75 0.2922724287938326 0.0021793697073295994 0.0 1.421328069997565E-4 4.737760233325216E-5 76-77 0.3452642770035751 0.0021793697073295994 0.0 1.421328069997565E-4 4.737760233325216E-5 78-79 0.40761320167413495 0.0021793697073295994 0.0 1.421328069997565E-4 4.737760233325216E-5 80-81 0.4915426242074912 0.0022267473096628516 0.0 1.421328069997565E-4 7.106640349987824E-5 82-83 0.5943520212706483 0.0022267473096628516 0.0 1.421328069997565E-4 9.475520466650432E-5 84-85 0.7162309032729395 0.0022267473096628516 0.0 1.421328069997565E-4 9.475520466650432E-5 86-87 0.8581505110621963 0.0022267473096628516 0.0 1.421328069997565E-4 9.475520466650432E-5 88 0.9792002850236556 0.002274124911996104 0.0 1.421328069997565E-4 9.475520466650432E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3020 0.0 85.88076 1 AACGTGT 1365 0.0 75.17528 1 CGTGTGG 1535 0.0 73.50181 3 ATCGAGT 1080 0.0 71.041985 1 ACGTGTG 1590 0.0 67.12362 2 TCGAGTG 1185 0.0 66.25898 2 GTGGAAT 54415 0.0 66.20658 6 TGGAATC 54890 0.0 65.571396 7 GGAATCA 55405 0.0 64.96884 8 GAATCAC 55500 0.0 64.857635 9 ACGAGTG 51720 0.0 63.81536 2 AACGAGT 47765 0.0 63.582237 1 AGTGGAA 54905 0.0 63.54533 5 CGAGTGG 53595 0.0 63.540314 3 GAGTGGA 55350 0.0 63.03444 4 AGCGAGT 665 0.0 51.67166 1 ATGAGTG 1830 0.0 50.099125 2 TGAGTGG 1945 0.0 49.79007 3 GCGAGTG 735 0.0 49.254967 2 AATGAGT 1685 0.0 49.165897 1 >>END_MODULE